Abstract:
:The ciliary kinase NEK8 plays a critical role in situs determination and cystic kidney disease, yet its exact function remains unknown. In this study, we identify ANKS6 as a target and activator of NEK8. ANKS6 requires NEK8 for localizing to the ciliary inversin compartment (IC) and activates NEK8 by binding to its kinase domain. Here we demonstrate the functional importance of this interaction through the analysis of two novel mouse mutations, Anks6(Streaker) and Nek8(Roc). Both display heterotaxy, cardiopulmonary malformations and cystic kidneys, a syndrome also characteristic of mutations in Invs and Nphp3, the other known components of the IC. The Anks6(Strkr) mutation decreases ANKS6 interaction with NEK8, precluding NEK8 activation. The Nek8(Roc) mutation inactivates NEK8 kinase function while preserving ANKS6 localization to the IC. Together, these data reveal the crucial role of NEK8 kinase activation within the IC, promoting proper left-right patterning, cardiopulmonary development and renal morphogenesis.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Czarnecki PG,Gabriel GC,Manning DK,Sergeev M,Lemke K,Klena NT,Liu X,Chen Y,Li Y,San Agustin JT,Garnaas MK,Francis RJ,Tobita K,Goessling W,Pazour GJ,Lo CW,Beier DR,Shah JVdoi
10.1038/ncomms7023subject
Has Abstractpub_date
2015-01-20 00:00:00pages
6023issn
2041-1723pii
ncomms7023journal_volume
6pub_type
杂志文章abstract::The majority of mammalian genes contain one or more alternative polyadenylation sites. Choice of polyadenylation sites was suggested as one of the underlying mechanisms for generating longer/shorter transcript isoforms. Here, we demonstrate that mature mRNA transcripts can undergo additional cleavage and polyadenylati...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02099-7
更新日期:2017-12-11 00:00:00
abstract::Quasicrystals, materials with aperiodic long-range order, have been found in intermetallics, soft materials such as colloids and supermolecules, and also in two-dimensional monolayer films. Here we present the first example of three-dimensional growth of a single-element quasicrystalline film. Using a hitherto unexplo...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3715
更新日期:2013-01-01 00:00:00
abstract::MCR-1 is a lipid A modifying enzyme that confers resistance to the antibiotic colistin. Here, we analyse the impact of MCR-1 expression on E. coli morphology, fitness, competitiveness, immune stimulation and virulence. Increased expression of mcr-1 results in decreased growth rate, cell viability, competitive ability ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02149-0
更新日期:2017-12-12 00:00:00
abstract::The continued convergence of electronics and photonics on the chip scale can benefit from the voltage control of optical polarization for applications in communications, signal processing and sensing. It is challenging, however, to electrically manipulate the polarization state of light in planar optical waveguides. H...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms6337
更新日期:2014-11-12 00:00:00
abstract::Transition metal-catalyzed C-H activation and radical reactions are two versatile strategies to construct diverse organic skeletons. Here we show the construction of a class of flavylium fluorophores via the merge of radical chemistry and C-H activation starting from (hetero)aryl ketones and alkynes. This protocol is ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13611-6
更新日期:2019-12-11 00:00:00
abstract::Generation and recombination of electrons and holes in organic solar cells occurs via charge transfer states located at the donor/acceptor interface. The energy of these charge transfer states is a crucial factor for the attainable open-circuit voltage and its correct determination is thus of utmost importance for a d...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-05987-8
更新日期:2018-09-07 00:00:00
abstract::Chronic bacterial infections on medical devices, including catheter-associated urinary tract infections (CAUTI), are associated with bacterial biofilm communities that are refractory to antibiotic therapy and resistant to host immunity. Previously, we have shown that Pseudomonas aeruginosa can cause CAUTI by forming a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06882-y
更新日期:2018-10-25 00:00:00
abstract::The negative phase of the Interdecadal Pacific Oscillation (IPO), a dominant mode of multi-decadal variability of sea surface temperatures (SSTs) in the Pacific, contributed to the reduced rate of global surface temperature warming in the early 2000s. A proposed mechanism for IPO multidecadal variability indicates tha...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11718
更新日期:2016-06-02 00:00:00
abstract::It is well known that a topologically protected gapless state appears at an interface between a topological insulator and an ordinary insulator; however, the physics of the interface between a topological insulator and a metal has largely been left unexplored. Here we report a novel phenomenon termed topological proxi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7547
更新日期:2015-03-12 00:00:00
abstract::Compartmentalization of the gut microbiota is thought to be important to system function, but the extent of spatial organization in the gut ecosystem remains poorly understood. Here, we profile the murine colonic microbiota along longitudinal and lateral axes using laser capture microdissection. We found fine-scale sp...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-12413-0
更新日期:2019-09-25 00:00:00
abstract::Diverse entry inhibitors targeting the gp120 subunit of the HIV-1 envelope (Env) trimer have been developed including BMS-626529, also called temsavir, a prodrug version of which is currently in phase III clinical trials. Here we report the characterization of a panel of small-molecule inhibitors including BMS-818251,...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-07851-1
更新日期:2019-01-03 00:00:00
abstract::A key element enabling the microelectronic technology advances of the past decades has been the conceptualization of complex circuits with versatile functionalities as being composed of the proper combination of basic 'lumped' circuit elements (for example, inductors and capacitors). In contrast, modern nanophotonic s...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms4896
更新日期:2014-05-29 00:00:00
abstract::Thanks to the nature of strong programmability, field-programmable gate arrays (FPGAs) have been playing a significant role in signal processing and control. With the explosive growth in digital data, big data analytics becomes an important emerging field, in which FPGAs are a major player. However, the computational ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-14249-0
更新日期:2020-01-21 00:00:00
abstract::Normal epithelial cells are stably connected to each other via the apical junctional complex (AJC). AJCs, however, tend to be disrupted during tumor progression, and this process is implicated in cancer dissemination. Here, using colon carcinoma cells that fail to form AJCs, we investigated molecular defects behind th...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-01945-y
更新日期:2017-11-28 00:00:00
abstract::Hox genes have been shown to be essential in vertebrate neural circuit formation and their depletion has resulted in homeotic transformations with neuron loss and miswiring. Here we quantifiy four eye movements in the zebrafish mutant valentino and hox3 knockdowns, and find that contrary to the classical model, oculom...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms5221
更新日期:2014-06-25 00:00:00
abstract::The adhesive wear process remains one of the least understood areas of mechanics. While it has long been established that adhesive wear is a direct result of contacting surface asperities, an agreed upon understanding of how contacting asperities lead to wear debris particle has remained elusive. This has restricted a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms11816
更新日期:2016-06-06 00:00:00
abstract::Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sen...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14791
更新日期:2017-03-24 00:00:00
abstract::Tunable metasurfaces enable dynamical control of the key constitutive properties of light at a subwavelength scale. To date, electrically tunable metasurfaces at near-infrared wavelengths have been realized using free carrier modulation, and switching of thermo-optical, liquid crystal and phase change media. However, ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11598-8
更新日期:2019-08-13 00:00:00
abstract::Brain oscillations reflect pattern formation of cell assemblies' activity, which is often disturbed in neurological and psychiatric diseases like depression, schizophrenia and stroke. In the neurobiological analysis and treatment of these conditions, transcranial electric currents applied to the brain proved beneficia...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3032
更新日期:2013-01-01 00:00:00
abstract::Increased vascular permeability contributes to many diseases, including acute respiratory distress syndrome, cancer and inflammation. Most past work on vascular barrier function has focused on soluble regulators, such as tumour-necrosis factor-α. Here we show that lung vascular permeability is controlled mechanically ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2774
更新日期:2013-01-01 00:00:00
abstract::An effective way to improve polymer solar cell efficiency is to use a tandem structure, as a broader part of the spectrum of solar radiation is used and the thermalization loss of photon energy is minimized. In the past, the lack of high-performance low-bandgap polymers was the major limiting factor for achieving high...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2411
更新日期:2013-01-01 00:00:00
abstract::The unusual electronic properties of diamondoids, the nanoscale relatives of diamond, make them attractive for applications ranging from drug delivery to field emission displays. Identifying the fundamental origin of these properties has proven highly challenging, with even the most advanced quantum many-body calculat...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3006
更新日期:2013-01-01 00:00:00
abstract::As European heatwaves become more severe, summers in the United Kingdom (UK) are also getting warmer. The UK record temperature of 38.7 °C set in Cambridge in July 2019 prompts the question of whether exceeding 40 °C is now within reach. Here, we show how human influence is increasing the likelihood of exceeding 30, 3...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-16834-0
更新日期:2020-06-30 00:00:00
abstract::Overcoming the restricted axonal regenerative ability that limits functional repair following a central nervous system injury remains a challenge. Here we report a regenerative paradigm that we call enriched conditioning, which combines environmental enrichment (EE) followed by a conditioning sciatic nerve axotomy tha...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20179-z
更新日期:2020-12-21 00:00:00
abstract::Despite the pursuit of quantum advantages in various applications, the power of quantum computers in executing neural network has mostly remained unknown, primarily due to a missing tool that effectively designs a neural network suitable for quantum circuit. Here, we present a neural network and quantum circuit co-des...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-20729-5
更新日期:2021-01-25 00:00:00
abstract::Chemical cross-linking of proteins coupled with mass spectrometry analysis (CXMS) is widely used to study protein-protein interactions (PPI), protein structures, and even protein dynamics. However, structural information provided by CXMS is still limited, partly because most CXMS experiments use lysine-lysine (K-K) cr...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11917-z
更新日期:2019-09-02 00:00:00
abstract::Moonlighting proteins are a subclass of multifunctional proteins whose functions are unrelated. Although they may play important roles in cells, there has been no large-scale method to identify them, nor any effort to characterize them as a group. Here, we propose the first method for the identification of 'extreme mu...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms8412
更新日期:2015-06-09 00:00:00
abstract::In eukaryotic cells, organellar proteome biogenesis is pivotal for cellular function. Chloroplasts contain a complex proteome, the biogenesis of which includes post-translational import of nuclear-encoded proteins. However, the mechanisms determining when and how nascent chloroplast-targeted proteins are sorted in the...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms7843
更新日期:2015-04-16 00:00:00
abstract::In vitro fabrication of functional vascularized three-dimensional tissues has been a long-standing objective in the field of tissue engineering. Here we report a technique to engineer cardiac tissues with perfusable blood vessels in vitro. Using resected tissue with a connectable artery and vein as a vascular bed, we ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2406
更新日期:2013-01-01 00:00:00
abstract::Insulin regulates glycaemia, lipogenesis and increases mRNA translation. Cells with reduced eukaryotic initiation factor 6 (eIF6) do not increase translation in response to insulin. The role of insulin-regulated translation is unknown. Here we show that reduction of insulin-regulated translation in mice heterozygous f...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9261
更新日期:2015-09-18 00:00:00