Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice.


AIM:To investigate the effect of Clostridium difficile (C. difficile) infection in an interleukin 10-deficient (IL-10(-/-)) mouse model of inflammatory bowel disease. METHODS:Bone marrow-derived dendritic cells isolated from wild type (WT) and IL-10(-/-)mice were stimulated for 4 h with C. difficile toxin A (200 μg/mL), and gene expression of interferon (IFN)-γ, IL-12 and IL-23 was determined by real-time reverse transcription polymerase chain reaction. WT and IL-10(-/-) mice (n = 20 each) were exposed to an antibiotic cocktail for three days and then were injected with clindamycin (i.p.). Mice (n = 10 WT, 10 IL-10(-/-)) were then challenged with oral administration of C. difficile (1 × 10(5) colony forming units of strain VPI 10463). Animals were monitored daily for 7 d for signs of colitis. Colonic tissue samples were evaluated for cytokine gene expression and histopathologic analysis. RESULTS:C. difficile toxin A treatment induced IFN-γ gene expression to a level that was significantly higher in BDMCs from IL-10(-/-) compared to those from WT mice (P < 0.05). However, expression of IL-12 and IL-23 was not different among the groups. Following C. difficile administration, mice developed diarrhea and lost weight within 2-3 d. Weight loss was significantly greater in IL-10(-/-) compared to WT mice (P < 0.05). C. difficile infection induced histopathologic features typical of colitis in both IL-10(-/-) and WT mice. The histopathologic severity score was significantly higher in the IL-10(-/-) than in WT mice (mean ± standard error; 5.50 ± 0.53 vs 2.44 ± 0.46; P < 0.05). This was accompanied by a significantly greater increase in IFN-γ gene expression in colonic tissues from IL-10(-/-) than from WT mice challenged with C. difficile (P < 0.05). CONCLUSION:These results indicate that colitis is more severe after C. difficile infection in IL-10(-/-)mice, and that IFN-γ expression is involved in this process.


World J Gastroenterol


Kim MN,Koh SJ,Kim JM,Im JP,Jung HC,Kim JS




Has Abstract


2014-12-07 00:00:00












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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Li J,Zhou R,Bie BB,Huang N,Guo Y,Chen HY,Shi MJ,Yang J,Zhang J,Li ZF

    更新日期:2018-01-07 00:00:00

  • Effect of bombesin and neurotensin on gut barrier function in partially hepatectomized rats.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Assimakopoulos SF,Alexandris IH,Scopa CD,Mylonas PG,Thomopoulos KC,Georgiou CD,Nikolopoulou VN,Vagianos CE

    更新日期:2005-11-21 00:00:00

  • Current research and treatment for gastrointestinal stromal tumors.

    abstract::Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract and have gained considerable research and treatment interest, especially in the last two decades. GISTs are driven by mutations commonly found in the KIT gene and less commonly in the platelet-derived growth fa...

    journal_title:World journal of gastroenterology

    pub_type: 社论,评审


    authors: Lim KT,Tan KY

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  • p53-expressing conditionally replicative adenovirus CNHK500-p53 against hepatocellular carcinoma in vitro.

    abstract:AIM:To develop a conditionally replicative gene-viral vector system called CNHK500-p53, which contains dual promoters within the E1 region, and combines the advantages of oncolytic virus and gene therapies for hepatocellular carcinoma (HCC). METHODS:CNHK500-p53 was constructed by using human telomerase reverse transcr...

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    pub_type: 杂志文章


    authors: Zhao HC,Zhang Q,Yang Y,Lu MQ,Li H,Xu C,Chen GH

    更新日期:2007-02-07 00:00:00

  • Multimodality magnetic resonance imaging in hepatic encephalopathy: an update.

    abstract::Hepatic encephalopathy (HE) is a neuropsychiatric complication of cirrhosis or acute liver failure. Currently, HE is regarded as a continuous cognitive impairment ranging from the mildest stage, minimal HE to overt HE. Hyperammonaemia and neuroinflammation are two main underlying factors which contribute to the neurol...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Zhang XD,Zhang LJ,Wu SY,Lu GM

    更新日期:2014-08-28 00:00:00

  • Splenectomy with endoscopic variceal ligation is superior to splenectomy with pericardial devascularization in treatment of portal hypertension.

    abstract:AIM:To investigate the therapeutic efficacy and complications of splenectomy with endoscopic variceal ligation (EVL) and splenectomy with pericardial devascularization (i.e. Hassab's operation) in patients with portal hypertension. METHODS:A total of 103 patients with liver cirrhosis and portal hypertension were rando...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Lin N,Liu B,Xu RY,Fang HP,Deng MH

    更新日期:2006-12-07 00:00:00

  • Association of Fusobacterium nucleatum infection with colorectal cancer in Chinese patients.

    abstract:AIM:To investigate Fusobacterium nucleatum (F. nucleatum) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients. METHODS:The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent ...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Li YY,Ge QX,Cao J,Zhou YJ,Du YL,Shen B,Wan YJ,Nie YQ

    更新日期:2016-03-21 00:00:00

  • Current status of function-preserving surgery for gastric cancer.

    abstract::Recent advances in diagnostic techniques have allowed the diagnosis of gastric cancer (GC) at an early stage. Due to the low incidence of lymph node metastasis and favorable prognosis in early GC, function-preserving surgery which improves postoperative quality of life may be possible. Pylorus-preserving gastrectomy (...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Saito T,Kurokawa Y,Takiguchi S,Mori M,Doki Y

    更新日期:2014-12-14 00:00:00

  • Association of hepatitis C virus infection and diabetes.

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    journal_title:World journal of gastroenterology

    pub_type: 评论,信件


    authors: Rouabhia S,Malek R,Bounecer H

    更新日期:2009-10-28 00:00:00

  • Relationship between entero-hepatic bile acid circulation and interdigestive migrating myoelectrical activity in rats.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Fang P,Dong L,Zhang WJ,Luo JY

    更新日期:2005-09-14 00:00:00

  • Preparation of monoclonal antibody against apoptosis-associated antigens of hepatoma cells by subtractive immunization.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Yang LJ,Wang WL

    更新日期:2002-10-01 00:00:00

  • Impact of lymph node micrometastasis in hilar bile duct carcinoma patients.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Taniguchi K,Iida T,Hori T,Yagi S,Imai H,Shiraishi T,Uemoto S

    更新日期:2006-04-28 00:00:00

  • Analysis of the urinary peptidome associated with Helicobacter pylori infection.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Xiao D,Meng FL,He LH,Gu YX,Zhang JZ

    更新日期:2011-02-07 00:00:00

  • Relationship between RGS5 expression and differentiation and angiogenesis of gastric carcinoma.

    abstract:AIM:To explore the regulator of G-protein signaling 5 (RGS5) expression in gastric carcinoma and its association with differentiation and microvascular density (MVD). METHODS:Expression of RGS5 and CD34 were examined in 76 cases of gastric carcinoma, including 22 cases with lymph node metastasis and 54 cases without l...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Wang JH,Huang WS,Hu CR,Guan XX,Zhou HB,Chen LB

    更新日期:2010-11-28 00:00:00

  • Zebrafish as a disease model for studying human hepatocellular carcinoma.

    abstract::Liver cancer is one of the world's most common cancers and the second leading cause of cancer deaths. Hepatocellular carcinoma (HCC), a primary hepatic cancer, accounts for 90%-95% of liver cancer cases. The pathogenesis of HCC consists of a stepwise process of liver damage that extends over decades, due to hepatitis,...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Lu JW,Ho YJ,Yang YJ,Liao HA,Ciou SC,Lin LI,Ou DL

    更新日期:2015-11-14 00:00:00

  • Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas.

    abstract:AIM:To investigate GATA5, SFRP2, and ITGA4 methylation in plasma DNA as noninvasive biomarkers for colorectal cancer (CRC) or adenomas. METHODS:There were 57 CRC patients, 30 adenomas patients, and 47 control patients enrolled in this study. Methylation-specific polymerase chain reaction was used to determine the prom...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Zhang X,Song YF,Lu HN,Wang DP,Zhang XS,Huang SL,Sun BL,Huang ZG

    更新日期:2015-03-07 00:00:00

  • A case of a duodenal duplication cyst presenting as melena.

    abstract::Duodenal duplication cysts are benign rare congenital anomalies reported mainly in the pediatric population, but seldom in adults. Symptoms depend on the type and location and can present as abdominal pain, distension, dysphagia or dyspepsia. They have been reported to be responsible for duodenal obstruction, pancreat...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Ko SY,Ko SH,Ha S,Kim MS,Shin HM,Baeg MK

    更新日期:2013-10-14 00:00:00

  • Successful resection of metachronous para-aortic, Virchow lymph node and liver metastatic recurrence of rectal cancer.

    abstract::A 66-year-old female presented with the main complaint of defecation trouble and abdominal distention. With diagnosis of rectal cancer, cSS, cN0, cH0, cP0, cM0 cStage II, Hartmann's operation with D3 lymph node dissection was performed and a para-aortic lymph node and a disseminated node near the primary tumor were re...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Takeshita N,Fukunaga T,Kimura M,Sugamoto Y,Tasaki K,Hoshino I,Ota T,Maruyama T,Tamachi T,Hosokawa T,Asai Y,Matsubara H

    更新日期:2015-11-28 00:00:00

  • Reprogramming of glucose metabolism in hepatocellular carcinoma: Progress and prospects.

    abstract::Hepatocellular carcinoma (HCC) is one of the most lethal cancers, and its rate of incidence is rising annually. Despite the progress in diagnosis and treatment, the overall prognoses of HCC patients remain dismal due to the difficulties in early diagnosis and the high level of tumor invasion, metastasis and recurrence...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Shang RZ,Qu SB,Wang DS

    更新日期:2016-12-07 00:00:00

  • Ulcerative colitis patients in clinical remission demonstrate correlations between fecal immunochemical test results, mucosal healing, and risk of relapse.

    abstract:AIM:To assess the risk of relapse in ulcerative colitis (UC) patients in clinical remission using mucosal status and fecal immunochemical test (FIT) results. METHODS:The clinical outcomes of 194 UC patients in clinical remission who underwent colonoscopy were based on evaluations of Mayo endoscopic subscores (MESs) an...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Nakarai A,Kato J,Hiraoka S,Takashima S,Takei D,Inokuchi T,Sugihara Y,Takahara M,Harada K,Okada H

    更新日期:2016-06-07 00:00:00

  • Comparative study of mutations in SNP loci of K-RAS, hMLH1 and hMSH2 genes in neoplastic intestinal polyps and colorectal cancer.

    abstract:AIM:To clarify the molecular mechanism involved in pathogenesis of colorectal cancer as well as clinical significance of genetic analysis of histological samples. METHODS:A total of 480 blood and tissue specimens were collected in our hospital from January 2011 to October 2012. In the observation group, there were 120...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Yan ZH,Cui LH,Wang XH,Li C,He X

    更新日期:2014-12-28 00:00:00

  • Transnasal endoscopic retrograde chalangiopancreatography using an ultrathin endoscope: a prospective comparison with a routine oral procedure.

    abstract:AIM:To investigate if transnasal endoscopic retrograde cholangiopancreatography (n-ERCP) using an ultrathin forward-viewing scope may overcome the disadvantages of conventional oral ERCP (o-ERCP) related to the large-caliber side-viewing duodenoscope. METHODS:The study involved 50 patients in whom 25 cases each were a...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Mori A,Ohashi N,Maruyama T,Tatebe H,Sakai K,Shibuya T,Inoue H,Takegoshi S,Okuno M

    更新日期:2008-03-14 00:00:00

  • Study of the influence of hiatus hernia on gastroesophageal reflux.

    abstract:AIM:To explore whether the presence of a sliding hiatus hernia influences gastroesophageal reflux. METHODS:Endoscopy and 24 h pH monitoring were performed for 197 outpatients with gastroesophageal reflux symptoms. RESULTS:Of the 197 patients with symptoms of gastroesophageal reflux, patients with hiatus hernia accoun...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Zhu HM

    更新日期:1997-03-15 00:00:00

  • Multifactorial analysis of recurrence of cholecy_stolithiasis in Shanghai area.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


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  • Gene modulation associated with inhibition of liver regeneration in hepatitis B virus X transgenic mice.

    abstract:AIM:To analyze the modulation of gene expression profile associated with inhibition of liver regeneration in hepatitis B X (HBx)-expressing transgenic mice. METHODS:Microarray technology was performed on liver tissue obtained from 4 control (LacZ) and 4 transgenic mice (HBx-LacZ), 48 h after partial hepatectomy. The s...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Sidorkiewicz M,Jais JP,Tralhao G,Morosan S,Giannini C,Brezillon N,Soussan P,Delpuech O,Kremsdorf D

    更新日期:2008-01-28 00:00:00

  • Characterisation of colonic dysplasia-like epithelial atypia in murine colitis.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Randall-Demllo S,Fernando R,Brain T,Sohal SS,Cook AL,Guven N,Kunde D,Spring K,Eri R

    更新日期:2016-10-07 00:00:00

  • α-fetoprotein, vascular endothelial growth factor receptor-1 and early recurrence of hepatoma.

    abstract:AIM:To investigate whether α-fetoprotein (AFP) and vascular endothelial growth factor receptor (VEGFR)-1 correlate with early recurrence of hepatoma/hepatocellular carcinoma (HCC). METHODS:From 2000 to 2005, 114 consecutive patients with HCC underwent primary curative hepatectomy. The mean age was 60.7 (8.7) years and...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Kamiyama T,Takahashi M,Nakanishi K,Yokoo H,Kamachi H,Kobayashi N,Ozaki M,Todo S

    更新日期:2012-01-28 00:00:00

  • Paraneoplastic hyperinsulinism and secondary hypoglycaemia in a patient with advanced colon cancer: a rare association.

    abstract::We review the case of a 74-year-old patient with advanced colon cancer who suffered recurrent bouts of hypoglycemia. A state of inappropriate, non-suppressed hyperinsulinism in the presence of severe hypoglycemia was diagnosed. We finally discuss the known mechanisms behind fasting hypoglycemia in patients with advanc...

    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Díaz R,Aparicio J,Mendizábal A,Faus M,Fleitas T,Aparisi F,Martín M

    更新日期:2008-03-28 00:00:00

  • Endoscopic trans-esophageal submucosal tunneling surgery: A new therapeutic approach for diseases located around the aorta ventralis.

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章


    authors: Xiong Y,Chen QQ,Chai NL,Jiao SC,Ling Hu EQ

    更新日期:2019-01-07 00:00:00

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    journal_title:World journal of gastroenterology

    pub_type: 杂志文章,评审


    authors: Shi JH,Line PD

    更新日期:2014-11-21 00:00:00