Reassignment of scattered emission photons in multifocal multiphoton microscopy.

Abstract:

:Multifocal multiphoton microscopy (MMM) achieves fast imaging by simultaneously scanning multiple foci across different regions of specimen. The use of imaging detectors in MMM, such as CCD or CMOS, results in degradation of image signal-to-noise-ratio (SNR) due to the scattering of emitted photons. SNR can be partly recovered using multianode photomultiplier tubes (MAPMT). In this design, however, emission photons scattered to neighbor anodes are encoded by the foci scan location resulting in ghost images. The crosstalk between different anodes is currently measured a priori, which is cumbersome as it depends specimen properties. Here, we present the photon reassignment method for MMM, established based on the maximum likelihood (ML) estimation, for quantification of crosstalk between the anodes of MAPMT without a priori measurement. The method provides the reassignment of the photons generated by the ghost images to the original spatial location thus increases the SNR of the final reconstructed image.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Cha JW,Singh VR,Kim KH,Subramanian J,Peng Q,Yu H,Nedivi E,So PT

doi

10.1038/srep05153

subject

Has Abstract

pub_date

2014-06-05 00:00:00

pages

5153

issn

2045-2322

pii

srep05153

journal_volume

4

pub_type

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