Abstract:
:The establishment of clinically relevant models for tumor metastasis and drug testing is a major challenge in cancer research. Here we report a physiologically relevant assay enabling quantitative analysis of metastatic capacity of tumor cells following implantation into the chorioallantoic membrane (CAM). Engraftment of as few as 103 non-small cell lung cancer (NSCLC) and prostate cancer (PCa) cell lines was sufficient for both primary tumor and metastasis formation. Standard 2D-imaging as well as 3D optical tomography imaging were used for the detection of fluorescent metastatic foci in the chick embryo. H2228- and H1975-initiated metastases were confirmed by genomic analysis. We quantified the inhibitory effect of docetaxel on LNCaP, and that of cisplatin on A549- and H1299-initiated metastatic growths. The CAM assay also mimicked the sensitivity of ALK-rearranged H2228 and EGFR-mutated H1975 NSCLC cells to tyrosine kinase inhibitors crizotinib and gefitinib respectively, as well as sensitivity of LNCaP cells to androgen-dependent enzalutamide therapy. The assay was suggested to reconstitute the bone metastatic tropism of PCa cells. We show that the CAM chick embryo model may be a powerful preclinical platform for testing and targeting of the metastatic capacity of cancer cells.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Pawlikowska P,Tayoun T,Oulhen M,Faugeroux V,Rouffiac V,Aberlenc A,Pommier AL,Honore A,Marty V,Bawa O,Lacroix L,Scoazec JY,Chauchereau A,Laplace-Builhe C,Farace Fdoi
10.1038/s41598-020-73632-wsubject
Has Abstractpub_date
2020-10-09 00:00:00pages
16876issue
1issn
2045-2322pii
10.1038/s41598-020-73632-wjournal_volume
10pub_type
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