Abstract：
:Heat shock protein 90 (HSP90) inhibition is an attractive strategy for cancer treatment. Several HSP90 inhibitors have shown promising effects in clinical oncology trials. However, little is known about HSP90 inhibition-mediated bladder cancer therapy. Here, we report a quantitative proteomic study that evaluates alterations in protein expression and histone post-translational modifications (PTMs) in bladder carcinoma in response to HSP90 inhibition. We show that 5 HSP90 inhibitors (AUY922, ganetespib, SNX2112, AT13387, and CUDC305) potently inhibited the proliferation of bladder cancer 5637 cells in a dose- and time-dependent manner. Our proteomic study quantified 518 twofold up-regulated and 811 twofold down-regulated proteins common to both AUY922 and ganetespib treatment. Bioinformatic analyses revealed that those differentially expressed proteins were involved in multiple cellular processes and enzyme-regulated signaling pathways, including chromatin modifications and cell death-associated pathways. Furthermore, quantitative proteome studies identified 14 types of PTMs with 93 marks on the core histones, including 34 novel histone marks of butyrylation, citrullination, 2-hydroxyisobutyrylation, methylation, O-GlcNAcylation, propionylation, and succinylation in AUY922- and ganetespib-treated 5637 cells. Together, this study outlines the association between proteomic changes and histone PTMs in response to HSP90 inhibitor treatment in bladder carcinoma cells, and thus intensifies the understanding of HSP90 inhibition-mediated bladder cancer therapeutics.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Li QQ,Hao JJ,Zhang Z,Krane LS,Hammerich KH,Sanford T,Trepel JB,Neckers L,Agarwal PKdoi
10.1038/s41598-017-00143-6subject
Has Abstractpub_date
2017-03-15 00:00:00pages
201issue
1issn
2045-2322pii
10.1038/s41598-017-00143-6journal_volume
7pub_type
杂志文章abstract：:A child with Down syndrome, like any other child, may benefit from interacting with memory stimuli, but needs additional support and help. The use of special teaching methods, which add playfulness and use of the computer, can enhance the memory processes of these children. In this work, we present the virtual environ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-60014-5
更新日期：2020-02-21 00:00:00
abstract：:On-surface synthesis provides a route for the production of 1D and 2D covalently bonded polymeric structures. Such reactions are confined to the surface of a substrate and the catalytic properties of the substrate are often utilised to initiate the reaction. Recent studies have focused on the properties of various cry...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45359-w
更新日期：2019-06-27 00:00:00
abstract：:Zika virus is a teratogenic mosquito-transmitted flavivirus that is associated with birth defects in newborns and Guillain-Barré syndrome in adults. The virus can also be sexually transmitted, but currently, very little is known about the cell types supporting virus replication and persistence in human testes. Using p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23899-x
更新日期：2018-04-03 00:00:00
abstract：:Contest behaviour, and in particular the propensity to attack an unfamiliar conspecific, is influenced by an individual's aggressiveness, as well as by experience of winning and losing (so called 'winner-loser effects'). Individuals vary in aggressiveness and susceptibility to winner-loser effects but the relationship...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69664-x
更新日期：2020-08-07 00:00:00
abstract：:The role of marginal populations for the long-term maintenance of species' genetic diversity and evolutionary potential is particularly timely in view of the range shifts caused by climate change. The Centre-Periphery hypothesis predicts that marginal populations should bear reduced genetic diversity and have low evol...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42416
更新日期：2017-02-10 00:00:00
abstract：:Large amounts of anthropogenic CO2 in the atmosphere are taken up by the ocean, which leads to 'ocean acidification' (OA). In addition, the increasing application of nanoparticles inevitably leads to their increased release into the aquatic environment. However, the impact of OA on the bioaccumulation of nanoparticles...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40047-1
更新日期：2019-03-05 00:00:00
abstract：:The Leading Edge Vortex (LEV) is a universal mechanism enhancing lift in flying organisms. LEVs, generally illustrated as a single vortex attached to the wing throughout the downstroke, have not been studied quantitatively in freely flying insects. Previous findings are either qualitative or from flappers and tethered...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03264
更新日期：2013-11-20 00:00:00
abstract：:Thymocyte and T cell trafficking relies on signals initiated by G-protein coupled receptors. To address the importance of the G-proteins Gαi2 and Gαi3 in thymocyte and T cell function, we developed several mouse models. Gαi2 deficiency in hematopoietic progenitors led to a small thymus, a double negative (DN)1/DN2 thy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04537-4
更新日期：2017-06-23 00:00:00
abstract：:The aesthetic value of landscapes contributes to human well-being. However, studies which have investigated the link between biodiversity and ecosystem services have not taken aesthetic value into account. In this study we evaluated how the aesthetics of coralligenous reefs, a key marine ecosystem in the Mediterranean...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34229
更新日期：2016-09-28 00:00:00
abstract：:This corrects the article DOI: 10.1038/srep18803. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/srep46987
更新日期：2018-05-22 00:00:00
abstract：:Pathogenic meningococci have acquired a 24 kb capsule synthesis island (cps) by horizontal gene transfer which consists of a synthetic locus and associated capsule transport genes flanked by repetitive Regions D and D'. Regions D and D' contain an intact gene encoding a UDP-galactose epimerase (galE1) and a truncated ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44442
更新日期：2017-03-14 00:00:00
abstract：:DNA focused panel sequencing has been rapidly adopted to assess therapeutic targets in advanced/refractory cancer. Integrated Genomic Profiling (IGP) utilising DNA/RNA with tumour/normal comparisons in a Clinical Laboratory Improvement Amendments (CLIA) compliant setting enables a single assay to provide: therapeutic ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-016-0021-4
更新日期：2016-12-23 00:00:00
abstract：:Fluorescence-based western blots are quantitative in principal, but require determining linear range for each antibody. Here, we use microwestern array to rapidly evaluate suitable conditions for quantitative western blotting, with up to 192 antibody/dilution/replicate combinations on a single standard size gel with a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29436-0
更新日期：2018-07-27 00:00:00
abstract：:The behavior of diatomic molecular solids under pressure have attracted great interest and been extensively studied. Under ambient pressure, the structure of bromine is known to be a molecular phase (phase I). With increasing pressure, it transforms into an incommensurate phase (phase V) before eventually to a monoato...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25649
更新日期：2016-05-09 00:00:00
abstract：:Whole-room indirect calorimeters (WRICs) have traditionally been used for real-time resting metabolic rate (RMR) measurements, while metabolic rate (MR) during short-interval exercises has commonly been measured by metabolic carts (MCs). This study aims to investigate the feasibility of incorporating short-interval ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71001-1
更新日期：2020-08-31 00:00:00
abstract：:In this study, a design methodology for a multi-optical probe confocal imaging system was developed. To develop an imaging system that has the required resolving power and imaging area, this study focused on a design methodology to create a scalable and easy-to-implement confocal imaging system. This system overcomes ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33278
更新日期：2016-09-12 00:00:00
abstract：:High-Q microresonator is perceived as a promising platform for optical frequency comb generation, via dissipative soliton formation. In order to achieve a higher quality factor and obtain the necessary anomalous dispersion, multi-mode waveguides were previously implemented in Si3N4 microresonators. However, coupling b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26255
更新日期：2016-05-16 00:00:00
abstract：:Interfacial Dzyaloshinskii-Moriya interaction (IDMI) is important for its roles in stabilizing the skyrmionic lattice as well as soliton-like domain wall motion leading towards new generation spintronic devices. However, achievement and detection of IDMI is often hindered by various spurious effects. Here, we demonstr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32592
更新日期：2016-09-02 00:00:00
abstract：:Electroceutical wound dressings, especially those involving current flow with silver based electrodes, show promise for treating biofilm infections. However, their mechanism of action is poorly understood. We have developed an in vitro agar based model using a bioluminescent strain of Pseudomonas aeruginosa to measure...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37891-y
更新日期：2019-02-14 00:00:00
abstract：:Wolbachia, an endosymbiotic alpha-proteobacterium commonly found in insects, can inhibit the transmission of human pathogens by mosquitoes. Biocontrol programs are underway using Aedes aegypti mosquitoes trans-infected with a non-natural Wolbachia strain to reduce dengue virus transmission. Less is known about the imp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70745-0
更新日期：2020-08-14 00:00:00
abstract：:Obesity is a progressive, chronic disease, which can be caused by long-term miscommunication between organs. It remains challenging to understand how chronic dysfunction in a particular tissue remotely impairs other organs to eventually imbalance organismal energy homeostasis. Here we introduce RNAi Pulse Induction (R...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43327-y
更新日期：2019-05-06 00:00:00
abstract：:Monocytes/macrophages have been found to be an important component of colitis. However, the key chemokine that initiates the CCR2+ monocytes migration from circulation to colitis tissue remains to be undiscovered. PC3-secreted microprotein (PSMP) is a novel chemokine whose receptor is CCR2. The physiological and patho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-05255-7
更新日期：2017-07-11 00:00:00
abstract：:We have analyzed the compositions of boron-carbon system, in which the [Formula: see text] compound is identified as structural stability at high pressure. The first-principles calculation is used to identify the phase diagram, electronic structure, and superconductivity of [Formula: see text]. Our results have demons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75049-x
更新日期：2020-10-22 00:00:00
abstract：:Although it has been reported that uterine signal transducer and activator of transcription 3 (STAT3) is essential for embryo implantation, the exact roles of uterine epithelial and stromal STAT3 on embryo implantation have not been elucidated. To address this issue, we generated Stat3-floxed/Ltf-iCre (Stat3-eKO), Sta...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72640-0
更新日期：2020-09-23 00:00:00
abstract：:Repeated cycles of post-remission high-dose cytarabine (Ara-C) have been suggested to improve survival in core binding factor (CBF) acute myeloid leukaemia (AML). High-dose Ara-C used for induction regimens has also been reported to be associated with increased treatment-related mortality (TRM). Few data are available...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57414-y
更新日期：2020-01-20 00:00:00
abstract：:Dementia is a neurocognitive disorder characterized by a progressive memory loss and impairment in cognitive and functional abilities. Autophagy and mitophagy are two important cellular processes by which the damaged intracellular components are degraded by lysosomes. To investigate the contribution of autophagy and m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56614-5
更新日期：2019-12-27 00:00:00
abstract：:Pre-clinical monitoring of tumor growth and identification of distal metastasis requires a balance between accuracy and expediency. Bioluminescence imaging (BLI) is often used to track tumor growth but is primarily limited to planar 2-dimensional (2D) imaging. Consistent subject placement within a standard top-mounted...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21668-4
更新日期：2018-02-20 00:00:00
abstract：:Hydrogen in the Earth's deep interior has been thought to exist as a hydroxyl group in high-pressure minerals. We present Muon Spin Rotation experiments on SiO2 stishovite, which is an archetypal high-pressure mineral. Positive muon (which can be considered as a light isotope of proton) implanted in stishovite was fou...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08437
更新日期：2015-02-13 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-46785-6
更新日期：2019-07-17 00:00:00
abstract：:Previous studies have evaluated gene expression in Alzheimer's disease (AD) brains to identify mechanistic processes, but have been limited by the size of the datasets studied. Here we have implemented a novel meta-analysis approach to identify differentially expressed genes (DEGs) in published datasets comprising 450...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep12393
更新日期：2015-07-23 00:00:00
