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Comparison of total and IgG ABO antibody titers in healthy individuals by using tube and column agglutination techniques.

Abstract：

BACKGROUND:Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reactions. Therefore, a method to measure antibodies, including IgG, is necessary. We investigated ABO antibody titers of healthy individuals using a column agglutination technique (CAT) with or without dithiothreitol (DTT) and compared them with titers obtained using a conventional tube method. METHODS:Among healthy adults who underwent a medical examination, 180 individuals (60 with blood group A, 60 with group B, and 60 with group O) were selected. Antibody titrations were performed using the immediate spin (IS) tube, anti-human globulin (AHG) tube, and CAT with or without DTT methods. RESULTS:Higher median values of anti-B and anti-A titers in groups A and B individuals, respectively, were obtained using the IS method than using the AHG method. Higher values for group O individuals were obtained using the AHG method. Higher median titers of anti-B and anti-A in group O individuals were obtained using CAT without DTT than using the AHG method. Median titers of anti-B and anti-A in all blood groups were higher in CAT without DTT than in CAT with DTT, especially for group O individuals. CONCLUSIONS:We recommend CAT with and without DTT for titration of anti-A and anti-B, especially in group O individuals, to provide more sensitive results that include IgG data. Adjustment of insurance coverage of fees associated with antibody titration might be necessary, considering the actual cost of reagents and personnel.

journal_name

Ann Lab Med

journal_title

Annals of laboratory medicine

authors

Park ES,Jo KI,Shin JW,Park R,Choi TY,Bang HI,Chai GR,Yun SG

doi

10.3343/alm.2014.34.3.223

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

223-9

issue

3

eissn

2234-3806

issn

2234-3814

journal_volume

34

pub_type

杂志文章

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