Single-color multitarget flow cytometry using monoclonal antibodies labeled with different intensities of the same fluorochrome.

Abstract:

BACKGROUND:We developed a single-color multitarget flow cytometry (SM-FC) assay, a single-tube assay with graded mean fluorescence intensities (MFIs). We evaluated the repeatability of SM-FC, and its correlation with multicolor flow cytometry (MFC), to assess its application as a routine FC assay. METHODS:We selected CD19, CD3, CD4, and CD8 as antigen targets to analyze a lymphocyte subset. MFIs were graded by adjusting monoclonal antibody (mAb) volumes to detect several cell populations. Dimly labeled mAb was prepared by decreasing mAb volume and the optimum diluted volume was determined by serial dilution. SM-FC repeatability was analyzed 10 times in 2 normal controls. The correlation between SM-FC and MFC was evaluated in 20 normal and 23 patient samples. RESULTS:CV values (0.8-5.0% and 1.3-4.1% in samples 1 and 2, respectively) acquired by SM-FC with CD3-fluorescein α-isothyocyanate (FITC)(dim)+CD4-FITC(bright) and with CD19-FITC(dim)+CD3-FITC(bright) showed good repeatability, comparable to that acquired by MFC (1.6-3.7% and 1.0-4.8% in samples 1 and 2, respectively). Excellent correlation was observed between the 2 methods in the 20 normal samples (B cells, T cells, non-T(helper) cells, and T(helper) cells; r(2)=0.87, 0.97, 0.97, and 0.98, respectively; P<0.05). There were also linear relationships between SM-FC with CD19-FITC(dim)+CD3-FITC(bright) and CD8-PE(dim)+CD4-PE(bright), and MFC, in the 23 patient samples (B cells, T cells, T(cytotoxic) cells, and T(helper) cells; r(2)≥0.98, 0.99, 0.99, and 0.99, respectively; P<0.05). CONCLUSIONS:The multicolor, single-tube SM-FC technique is a potential alternative tool for identifying a lymphocyte subset.

journal_name

Ann Lab Med

authors

Park J,Han K

doi

10.3343/alm.2012.32.3.171

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

171-6

issue

3

eissn

2234-3806

issn

2234-3814

journal_volume

32

pub_type

杂志文章
  • Erratum: COVID-19 Molecular Testing in Korea: Practical Essentials and Answers From Experts Based on Experiences of Emergency Use Authorization Assays.

    abstract::[This corrects the article on p. 439 in vol. 40.]. ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章,已发布勘误

    doi:10.3343/alm.2021.41.1.126

    authors: Sung H,Roh KH,Hong KH,Seong MW,Ryoo N,Kim HS,Lee J,Kim SY,Ryu SW,Kim MN,Han MG,Lee SW,Lee H,Yoo CK

    更新日期:2021-01-01 00:00:00

  • The 2016 WHO versus 2008 WHO Criteria for the Diagnosis of Chronic Myelomonocytic Leukemia.

    abstract::The 2016 WHO diagnostic criteria for chronic myelomonocytic leukemia (CMML) require both absolute and relative monocytosis (≥1×10⁹/L and ≥10% of white blood cell counts) in peripheral blood. Moreover, myeloproliferative neoplasm (MPN) features in bone marrow and/or MPN-associated mutations tend to support MPN with mon...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2018.38.5.481

    authors: Moon Y,Kim MH,Kim HR,Ahn JY,Huh J,Huh JY,Han JH,Park JS,Cho SR

    更新日期:2018-09-01 00:00:00

  • Effectiveness of Plasma and Urine Neutrophil Gelatinase-Associated Lipocalin for Predicting Acute Kidney Injury in High-Risk Patients.

    abstract:BACKGROUND:Neutrophil gelatinase-associated lipocalin (NGAL) is a useful biomarker for acute kidney injury (AKI) prediction. However, studies on whether using both plasma NGAL (PNGAL) and urine NGAL (UNGAL) can improve AKI prediction are limited. We investigated the best approach to predict AKI in high-risk patients wh...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2021.41.1.60

    authors: Yi A,Lee CH,Yun YM,Kim H,Moon HW,Hur M

    更新日期:2021-01-01 00:00:00

  • Duffy blood group genotyping in Thai blood donors.

    abstract:BACKGROUND:Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequen...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.6.618

    authors: Nathalang O,Intharanut K,Siriphanthong K,Nathalang S,Kupatawintu P

    更新日期:2015-11-01 00:00:00

  • Stability of pH, Blood Gas Partial Pressure, Hemoglobin Oxygen Saturation Fraction, and Lactate Concentration.

    abstract:BACKGROUND:The storage temperature and time of blood gas samples collected in syringes constitute preanalytical variables that could affect blood gas or lactate concentration measurement results. We analyzed the effect of storage temperature and time delay on arterial or venous blood gas stability related to pH, partia...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2020.40.6.448

    authors: Arbiol-Roca A,Imperiali CE,Dot-Bach D,Valero-Politi J,Dastis-Arias M

    更新日期:2020-11-01 00:00:00

  • The first Korean case of candidemia due to Candida dubliniensis.

    abstract::Candidemia due to uncommon Candida spp. appears to be increasing in incidence. C. dubliniensis has been increasingly recovered from individuals not infected with HIV. Identification of C. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to C. albicans. We report the first ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.3.225

    authors: Yu N,Kim HR,Lee MK

    更新日期:2012-05-01 00:00:00

  • Neopterin Levels and Indoleamine 2,3-Dioxygenase Activity as Biomarkers of Immune System Activation and Childhood Allergic Diseases.

    abstract:BACKGROUND:Although Th2 immune activation is predominant in allergic diseases, neopterinlevels and indoleamine 2,3-dioxygenase (IDO)-1 activity (kynurenine:tryptophan ratio), which reflect Th1 immune activity, increase with interferon-gamma (IFN-γ) stimulation. We investigated neopterin, tryptophan, and kynurenine leve...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2019.39.3.284

    authors: Ünüvar S,Erge D,Kılıçarslan B,Gözükara Bağ HG,Çatal F,Girgin G,Baydar T

    更新日期:2019-05-01 00:00:00

  • Evaluation of PCR-reverse blot hybridization assay, REBA Sepsis-ID test, for simultaneous identification of bacterial pathogens and mecA and van genes from blood culture bottles.

    abstract:BACKGROUND:The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. METHODS:One thousand four hundred consecutive blood cult...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2014.34.6.446

    authors: Park SD,Lee G,Wang HY,Park M,Kim S,Kim H,Kim J,Kim YK,Kim HY,Lee H,Uh Y,Kim JB

    更新日期:2014-11-01 00:00:00

  • Establishing reference intervals for sex hormones on the analytical platforms Advia Centaur and Immulite 2000XP.

    abstract::Reliable reference intervals for sex hormones are indispensable in evaluations of the hypothalamo-pituitary-gonadal axis. This study established reference intervals for estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, and prolactin with the immunoassay platforms Advia Centaur and Immulite 20...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2016.36.1.55

    authors: Schüring AN,Kelsch R,Pierściński G,Nofer JR

    更新日期:2016-01-01 00:00:00

  • Antibiotic resistance in Helicobacter pylori strains and its effect on H. pylori eradication rates in a single center in Korea.

    abstract:BACKGROUND:Clarithromycin, amoxicillin, metronidazole, tetracycline, and levofloxacin have been commonly used for the eradication of Helicobacter pylori. We compared the change in antibiotic resistance of H. pylori strains during two separate periods and investigated the effect of antibiotic resistance on H. pylori era...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2013.33.6.415

    authors: An B,Moon BS,Kim H,Lim HC,Lee YC,Lee G,Kim SH,Park M,Kim JB

    更新日期:2013-11-01 00:00:00

  • Comparison Between Soluble ST2 and High-Sensitivity Troponin I in Predicting Short-Term Mortality for Patients Presenting to the Emergency Department With Chest Pain.

    abstract:BACKGROUND:High-sensitivity cardiac troponin I (hs-cTnI) and the soluble isoform of suppression of tumorigenicity 2 (sST2) are useful prognostic biomarkers in acute coronary syndrome (ACS). The aim of this study was to test the short term prognostic value of sST2 compared with hs-cTnI in patients with chest pain. METH...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2017.37.2.137

    authors: Marino R,Magrini L,Orsini F,Russo V,Cardelli P,Salerno G,Hur M,Di Somma S,GREAT NETWORK.

    更新日期:2017-03-01 00:00:00

  • Characterization of Extended-Spectrum β-Lactamase Genes of Shigella flexneri Isolates With Fosfomycin Resistance From Patients in China.

    abstract:BACKGROUND:The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flex...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2017.37.5.415

    authors: Liu Y,Cheng Y,Yang H,Hu L,Cheng J,Ye Y,Li J

    更新日期:2017-09-01 00:00:00

  • Plasma Levels and Diagnostic Utility of Macrophage Colony-Stimulating Factor, Matrix Metalloproteinase-9, and Tissue Inhibitor of Metalloproteinases-1 as New Biomarkers of Breast Cancer.

    abstract:BACKGROUND:Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-9 (MMP-9), and its specific tissue inhibitor - tissue inhibitor of metalloproteinases-1 (TIMP-1) may play an important role in the pathogenesis and spread of cancer. We investigated the plasma levels of M-CSF, MMP-9, and TIMP-1 in compari...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2016.36.3.223

    authors: Ławicki S,Głażewska EK,Sobolewska M,Będkowska GE,Szmitkowski M

    更新日期:2016-05-01 00:00:00

  • The impact of HLA and KIR ligand mismatching on unrelated allogeneic hematopoietic stem cell transplantation in Korean adult patients.

    abstract:BACKGROUND:The impact of HLA and KIR ligand mismatching on the outcome of hematopoietic stem cell transplantation (HSCT) remains unclear. Previous reports have identified considerable ethnic differences in the impact of HLA and KIR ligand mismatches, as well as KIR ligand status, on HSCT; however, to date, no data has ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.1.111

    authors: Park H,Rho EY,In JW,Kim I,Yoon SS,Park S,Shin S,Park KU,Song EY

    更新日期:2015-01-01 00:00:00

  • Biochemical and Genetic Analysis of Seven Korean Individuals With Suspected Metachromatic Leukodystrophy.

    abstract::Metachromatic leukodystrophy (MLD) is an autosomal recessive disease caused by a deficiency in arylsulfatase A (ARSA). However, decreased ARSA activity is also observed in pseudodeficiency (PD). To distinguish between MLD and PD, we performed gene mutation and sulfatide analyses by using dried blood spots (DBSs) from ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.4.458

    authors: Han M,Jun SH,Lee YJ,Eun BL,Lee SJ,Seong MW,Park SS,Song SH,Park HD,Song J

    更新日期:2015-07-01 00:00:00

  • New decision criteria for selecting delta check methods based on the ratio of the delta difference to the width of the reference range can be generally applicable for each clinical chemistry test item.

    abstract:BACKGROUND:Many laboratories use 4 delta check methods: delta difference, delta percent change, rate difference, and rate percent change. However, guidelines regarding decision criteria for selecting delta check methods have not yet been provided. We present new decision criteria for selecting delta check methods for e...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.5.345

    authors: Park SH,Kim SY,Lee W,Chun S,Min WK

    更新日期:2012-09-01 00:00:00

  • Galectin-3 Reflects the Echocardiographic Grades of Left Ventricular Diastolic Dysfunction.

    abstract:BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2018.38.4.306

    authors: Ansari U,Behnes M,Hoffmann J,Natale M,Fastner C,El-Battrawy I,Rusnak J,Kim SH,Lang S,Hoffmann U,Bertsch T,Borggrefe M,Akin I

    更新日期:2018-07-01 00:00:00

  • Use of PCR with sequence-specific primers for high-resolution human leukocyte antigen typing of patients with narcolepsy.

    abstract:BACKGROUND:Narcolepsy is a neurologic disorder characterized by excessive daytime sleepiness, symptoms of abnormal rapid eye movement (REM) sleep, and a strong association with HLA-DRB1*1501, -DQA1*0102, and -DQB1*0602. Here, we investigated the clinico-physical characteristics of Korean patients with narcolepsy, their...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.1.57

    authors: Woo HI,Joo EY,Hong SB,Lee KW,Kang ES

    更新日期:2012-01-01 00:00:00

  • A case of IgM deficiency with B cell deficiency detected by ABO discrepancy in a patient with acute osteomyelitis.

    abstract::ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2013.33.3.208

    authors: Jung CL,Cha MK,Jun BH,Hong KS

    更新日期:2013-05-01 00:00:00

  • Highly sensitive and novel point-of-care system, aQcare Chlamydia TRF kit for detecting Chlamydia trachomatis by using europium (Eu) (III) chelated nanoparticles.

    abstract:BACKGROUND:The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2015.35.1.50

    authors: Ham JY,Jung J,Hwang BG,Kim WJ,Kim YS,Kim EJ,Cho MY,Hwang MS,Won DI,Suh JS

    更新日期:2015-01-01 00:00:00

  • Evaluation of Xpert Carba-R Assay v.2 to Detect Carbapenemase Genes in Two Hospitals in Korea.

    abstract:BACKGROUND:As the spread of carbapenemase-producing Enterobacteriaceae poses a critical threat to public health, rapid detection of carbapenemase genes is urgently required for prompt initiation of appropriate antimicrobial therapy and infection control. We evaluated the performance of Xpert Carba-R v.2 (Cepheid, USA) ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2020.40.3.209

    authors: Byun JH,Kim YA,Kim M,Kim B,Choi JY,Park YS,Yong D

    更新日期:2020-05-01 00:00:00

  • Evaluation of Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile Assays for Direct Detection of Toxigenic Clostridium difficile in Stool Specimens.

    abstract:BACKGROUND:We evaluated the performance of four commercial nucleic acid amplification tests (NAATs: Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile) for direct and rapid detection of Clostridium difficile toxin genes. METHODS:We compared four NAATs on the same set of 3...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2016.36.2.131

    authors: Shin BM,Yoo SM,Shin WC

    更新日期:2016-03-01 00:00:00

  • Prevalence of blaZ Gene and Performance of Phenotypic Tests to Detect Penicillinase in Staphylococcus aureus Isolates from Japan.

    abstract::Guidelines recommend that clinical laboratories perform phenotypic tests (nitrocefin-based test and penicillin 10-U [P10] or 1-U [P1] zone edge tests) to detect penicillinase in Staphylococcus aureus isolates. This study aimed to assess the prevalence of blaZ encoding penicillinase and perform various phenotypic tests...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2018.38.2.155

    authors: Takayama Y,Tanaka T,Oikawa K,Fukano N,Goto M,Takahashi T

    更新日期:2018-03-01 00:00:00

  • Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana benthamiana.

    abstract:BACKGROUND:Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2017.37.4.313

    authors: Mazalovska M,Varadinov N,Koynarski T,Minkov I,Teoharov P,Lomonossoff GP,Zahmanova G

    更新日期:2017-07-01 00:00:00

  • Clonal spread of carbapenem non-susceptible Acinetobacter baumannii in an intensive care unit in a teaching hospital in China.

    abstract:BACKGROUND:This study was aimed to investigate the genetic diversity and antibiotic resistance profile of the nosocomial infection agent Acinetobacter baumannii from a medical intensive care unit (ICU) in a teaching hospital in Suzhou, China. METHODS:The genetic relationship among A. baumannii isolates in an ICU was i...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.6.413

    authors: Zhong Q,Xu W,Wu Y,Xu H

    更新日期:2012-11-01 00:00:00

  • A Unique Mutational Spectrum of MLC1 in Korean Patients With Megalencephalic Leukoencephalopathy With Subcortical Cysts: p.Ala275Asp Founder Mutation and Maternal Uniparental Disomy of Chromosome 22.

    abstract:BACKGROUND:Megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a rare inherited disorder characterized by infantile-onset macrocephaly, slow neurologic deterioration, and seizures. Mutations in the causative gene, MLC1, are found in approximately 75% of patients and are inherited in an autosomal recessi...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2017.37.6.516

    authors: Choi SA,Kim SY,Yoon J,Choi J,Park SS,Seong MW,Kim H,Hwang H,Choi JE,Chae JH,Kim KJ,Kim S,Lee YJ,Nam SO,Lim BC

    更新日期:2017-11-01 00:00:00

  • Update on procalcitonin measurements.

    abstract::Procalcitonin (PCT) is used as a biomarker for the diagnosis of sepsis, severe sepsis and septic shock. At the same time, PCT has also been used to guide antibiotic therapy. This review outlines the main indications for PCT measurement and points out possible pitfalls. The classic indications for PCT measurement are: ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.3343/alm.2014.34.4.263

    authors: Meisner M

    更新日期:2014-07-01 00:00:00

  • Budget Impact of the Accreditation Program for Clinical Laboratories on Colorectal Cancer Screening via Fecal Immunochemical Testing: Results from the National Cancer Screening Program in Korea.

    abstract:BACKGROUND:False-positive fecal immunochemical test (FIT) results in the National Cancer Screening Program (NCSP) for colorectal cancer may lead to unnecessary procedures, such as colonoscopies, increasing the medical costs. We estimated reductions in the cost of National Health Insurance according to the accreditation...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2018.38.3.249

    authors: Jun JK,Sung NY,Song SH,Hong S,Jang MA,Song J,Kim JH,Min WK,Lee YK

    更新日期:2018-05-01 00:00:00

  • Role of plasma exchange in ABO-incompatible kidney transplantation.

    abstract:BACKGROUND:In the past, ABO incompatibility was an absolute contraindication for solid organ transplantation. However, multiple recent trials have suggested strategies for overcoming the reactions between graft antigens and recipient antibodies that cause graft rejection. In this study, we determined the usefulness of ...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2012.32.4.283

    authors: Yoo S,Lee EY,Huh KH,Kim MS,Kim YS,Kim HO

    更新日期:2012-07-01 00:00:00

  • Comparison of total and IgG ABO antibody titers in healthy individuals by using tube and column agglutination techniques.

    abstract:BACKGROUND:Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reactions. Therefore, a method to measure antibodies, including IgG, is necessary. We investigated ABO antibody titers of healthy individuals using a column ag...

    journal_title:Annals of laboratory medicine

    pub_type: 杂志文章

    doi:10.3343/alm.2014.34.3.223

    authors: Park ES,Jo KI,Shin JW,Park R,Choi TY,Bang HI,Chai GR,Yun SG

    更新日期:2014-05-01 00:00:00