当前位置： SCI文献检索 > Annals of Laboratory Medicine期刊下所有文献 > Antimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections in Turkey.

Antimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections in Turkey.

Abstract：

:We aimed to observe antimicrobial resistance patterns and integron carriage of Escherichia coli isolates causing community-acquired infections. Two hundred sixty-eight E. coli strains were obtained from outpatients with various infections at different polyclinics at the 82nd Year of State Hospital in Rize, Turkey. Susceptibility to antimicrobials was tested using a disk diffusion method. The presence of integrons was examined using PCR with specific primers. Positive PCR results were confirmed by sequencing. A broth mating method was used for conjugation assays. Extragenic palindromic-PCR was performed using the oligonucleotide primer BOXA1R. Resistance frequency for ampicillin, trimethoprim/sulfamethoxazole, and tetracycline was determined as 50.6%, 33.5%, and 36.8% respectively. No strains were resistant to amikacin. Seventy isolates were positive for the intI1 gene, of which 49 carried gene cassettes. Eleven isolates were positive for the intI2 gene, eight of which carried gene cassettes. Seven gene cassettes (dfrA1, dfrA5, dfrA7, dfrA17, aadA1, aadA5, and sat2) were predominantly harbored in integrons. We detected conjugative plasmids harboring integrons in two E. coli strains. Four strain clusters were yielded by BOX-PCR fingerprints showing that they were clonally related. No apparent relationship occurred among class 1 and 2 integron-carrying strains. We conclude that integrons are widespread in genetically variable E. coli strains and will continue to mediate dissemination of resistance genes in the community.

journal_name

Ann Lab Med

journal_title

Annals of laboratory medicine

authors

Copur-Cicek A,Ozgumus OB,Saral A,Sandalli C

doi

10.3343/alm.2014.34.2.139

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

139-44

issue

2

eissn

2234-3806

issn

2234-3814

journal_volume

34

pub_type

杂志文章

相关文献

Annals of Laboratory Medicine文献大全
  • Single-color multitarget flow cytometry using monoclonal antibodies labeled with different intensities of the same fluorochrome.

    abstract：BACKGROUND:We developed a single-color multitarget flow cytometry (SM-FC) assay, a single-tube assay with graded mean fluorescence intensities (MFIs). We evaluated the repeatability of SM-FC, and its correlation with multicolor flow cytometry (MFC), to assess its application as a routine FC assay. METHODS:We selected ...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2012.32.3.171

    authors： Park J,Han K

    更新日期：2012-05-01 00:00:00

  • Stability of pH, Blood Gas Partial Pressure, Hemoglobin Oxygen Saturation Fraction, and Lactate Concentration.

    abstract：BACKGROUND:The storage temperature and time of blood gas samples collected in syringes constitute preanalytical variables that could affect blood gas or lactate concentration measurement results. We analyzed the effect of storage temperature and time delay on arterial or venous blood gas stability related to pH, partia...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2020.40.6.448

    authors： Arbiol-Roca A,Imperiali CE,Dot-Bach D,Valero-Politi J,Dastis-Arias M

    更新日期：2020-11-01 00:00:00

  • Clinical and microbiological characteristics of six Staphylococcus pettenkoferi isolates from blood samples.

    abstract：:Coagulase-negative staphylococci (CoNS) are reported to be the leading cause of nosocomial bloodstream infections. Staphylococcus pettenkoferi is a novel member of CoNS that was first isolated from the human blood and bursitis wound in 2002. We have reported cases of 6 S. pettenkoferi strains isolated from blood speci...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2015.35.2.250

    authors： Park S,Chung HS,Lee M

    更新日期：2015-03-01 00:00:00

  • Prevalence of blaZ Gene and Performance of Phenotypic Tests to Detect Penicillinase in Staphylococcus aureus Isolates from Japan.

    abstract：:Guidelines recommend that clinical laboratories perform phenotypic tests (nitrocefin-based test and penicillin 10-U [P10] or 1-U [P1] zone edge tests) to detect penicillinase in Staphylococcus aureus isolates. This study aimed to assess the prevalence of blaZ encoding penicillinase and perform various phenotypic tests...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2018.38.2.155

    authors： Takayama Y,Tanaka T,Oikawa K,Fukano N,Goto M,Takahashi T

    更新日期：2018-03-01 00:00:00

  • A Population-Based Genomic Study of Inherited Metabolic Diseases Detected Through Newborn Screening.

    abstract：BACKGROUND:A newborn screening (NBS) program has been utilized to detect asymptomatic newborns with inherited metabolic diseases (IMDs). There have been some bottlenecks such as false-positives and imprecision in the current NBS tests. To overcome these issues, we developed a multigene panel for IMD testing and investi...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2016.36.6.561

    authors： Park KJ,Park S,Lee E,Park JH,Park JH,Park HD,Lee SY,Kim JW

    更新日期：2016-11-01 00:00:00

  • Interlaboratory Comparison of the Results of Lifecodes LSA Class I and Class II Single Antigen Kits for Human Leukocyte Antigen Antibody Detection.

    abstract：BACKGROUND:Although single antigen bead assays (SAB) are approved qualitative tests, the median fluorescence intensity (MFI) values obtained from SAB are frequently used in combination with quantitative significances for diagnostic purposes. To gauge the reproducibility of SAB results, we assessed the interlaboratory v...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2015.35.3.321

    authors： Oh EJ,Park H,Park KU,Kang ES,Kim HS,Song EY

    更新日期：2015-05-01 00:00:00

  • Clonal Distribution of Clindamycin-Resistant Erythromycin-Susceptible (CRES) Streptococcus agalactiae in Korea Based on Whole Genome Sequences.

    abstract：BACKGROUND:The clindamycin-resistant erythromycin-susceptible (CRES) phenotype is rare in Streptococcus agalactiae (group B streptococci). We aimed to determine the molecular characteristics of CRES S. agalactiae using whole genome sequencing (WGS). METHODS:Sixty-six S. agalactiae isolates obtained from blood (N=26), ...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2020.40.5.370

    authors： Takahashi T,Maeda T,Lee S,Lee DH,Kim S

    更新日期：2020-09-01 00:00:00

  • Point of care D-dimer testing in the emergency department: a bioequivalence study.

    abstract：BACKGROUND:D-dimer is used widely as a diagnostic aid in low- and moderate-risk patients with suspected venous thromboembolism (VTE). While our laboratory utilizes VIDAS D-dimer analyzer (bioMérieux SA, France), our emergency department (ED) recently procured a D-dimer analyzer AQT90 FLEX (Radiometer Medical ApS, Denma...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2013.33.1.34

    authors： Perveen S,Unwin D,Shetty AL

    更新日期：2013-01-01 00:00:00

  • Comparison Between Soluble ST2 and High-Sensitivity Troponin I in Predicting Short-Term Mortality for Patients Presenting to the Emergency Department With Chest Pain.

    abstract：BACKGROUND:High-sensitivity cardiac troponin I (hs-cTnI) and the soluble isoform of suppression of tumorigenicity 2 (sST2) are useful prognostic biomarkers in acute coronary syndrome (ACS). The aim of this study was to test the short term prognostic value of sST2 compared with hs-cTnI in patients with chest pain. METH...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2017.37.2.137

    authors： Marino R,Magrini L,Orsini F,Russo V,Cardelli P,Salerno G,Hur M,Di Somma S,GREAT NETWORK.

    更新日期：2017-03-01 00:00:00

  • Screening PCR Versus Sanger Sequencing: Detection of CALR Mutations in Patients With Thrombocytosis.

    abstract：BACKGROUND:Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequ...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2016.36.4.291

    authors： Jeong JH,Lee HT,Seo JY,Seo YH,Kim KH,Kim MJ,Lee JH,Park J,Hong JS,Park PW,Ahn JY

    更新日期：2016-07-01 00:00:00

  • Immune Checkpoint Programmed Cell Death Protein-1 (PD-1) Expression on Bone Marrow T Cell Subsets in Patients With Plasma Cell Myeloma.

    abstract：Background:Plasma cell myeloma (PCM) is caused by immune dysregulation. We evaluated the expression of immune checkpoint programmed cell death protein-1 (PD-1) on T cell subsets in PCM patients according to disease course and cytogenetic abnormalities. This study aimed to find a target group suitable for therapeutic us...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2021.41.3.259

    authors： Lee MY,Park CJ,Cho YU,You E,Jang S,Seo EJ,Lee JH,Yoon DH,Suh C

    更新日期：2021-05-01 00:00:00

  • A Unique Mutational Spectrum of MLC1 in Korean Patients With Megalencephalic Leukoencephalopathy With Subcortical Cysts: p.Ala275Asp Founder Mutation and Maternal Uniparental Disomy of Chromosome 22.

    abstract：BACKGROUND:Megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a rare inherited disorder characterized by infantile-onset macrocephaly, slow neurologic deterioration, and seizures. Mutations in the causative gene, MLC1, are found in approximately 75% of patients and are inherited in an autosomal recessi...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2017.37.6.516

    authors： Choi SA,Kim SY,Yoon J,Choi J,Park SS,Seong MW,Kim H,Hwang H,Choi JE,Chae JH,Kim KJ,Kim S,Lee YJ,Nam SO,Lim BC

    更新日期：2017-11-01 00:00:00

  • Characterization of Extended-Spectrum β-Lactamase Genes of Shigella flexneri Isolates With Fosfomycin Resistance From Patients in China.

    abstract：BACKGROUND:The emergence of fosfomycin resistance and extended-spectrum β-lactamase (ESBL) genes is a serious threat to public health and a new challenge in shigellosis treatment. The purpose of this study was to identify fosfomycin resistance and characterize β-lactamase genes in fos-carrying isolates of Shigella flex...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2017.37.5.415

    authors： Liu Y,Cheng Y,Yang H,Hu L,Cheng J,Ye Y,Li J

    更新日期：2017-09-01 00:00:00

  • The first Korean case of candidemia due to Candida dubliniensis.

    abstract：:Candidemia due to uncommon Candida spp. appears to be increasing in incidence. C. dubliniensis has been increasingly recovered from individuals not infected with HIV. Identification of C. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to C. albicans. We report the first ...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2012.32.3.225

    authors： Yu N,Kim HR,Lee MK

    更新日期：2012-05-01 00:00:00

  • Platelet Function Analyzer-200 P2Y Results Are Predictive of the Risk of Major Adverse Cardiac Events in Korean Patients Receiving Clopidogrel Therapy Following Acute Coronary Syndrome.

    abstract：BACKGROUND:Clopidogrel is one of the most commonly used anti-platelet agents in cardiovascular diseases. We analyzed the relationship between the platelet function analyzer (PFA)-200 P2Y (INNOVANCE PFA-200 System, Siemens Healthcare, Germany) results and occurrence of major adverse cardiac events (MACEs) in Korean pati...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2018.38.5.413

    authors： Lim HH,Li S,An GD,Woo KS,Kim KH,Kim JM,Kim MH,Han JY

    更新日期：2018-09-01 00:00:00

  • Performance of an Automated Fluorescence Antinuclear Antibody Image Analyzer.

    abstract：BACKGROUND:The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2017.37.3.240

    authors： Yoo IY,Oh JW,Cha HS,Koh EM,Kang ES

    更新日期：2017-05-01 00:00:00

  • Highly sensitive and novel point-of-care system, aQcare Chlamydia TRF kit for detecting Chlamydia trachomatis by using europium (Eu) (III) chelated nanoparticles.

    abstract：BACKGROUND:The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2015.35.1.50

    authors： Ham JY,Jung J,Hwang BG,Kim WJ,Kim YS,Kim EJ,Cho MY,Hwang MS,Won DI,Suh JS

    更新日期：2015-01-01 00:00:00

  • Impact of genetic abnormalities on the prognoses and clinical parameters of patients with multiple myeloma.

    abstract：BACKGROUND:We reviewed patients with multiple myeloma (MM) in order to assess the incidence of genetic abnormalities and their associations with clinical parameters, risk groups, and prognosis. METHODS:A total of 130 patients with MM were enrolled. The incidences of genetic abnormalities were determined in all patient...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2013.33.4.248

    authors： Jekarl DW,Min CK,Kwon A,Kim H,Chae H,Kim M,Lim J,Kim Y,Han K

    更新日期：2013-07-01 00:00:00

  • Comparison of ChromID agar and Clostridium difficile selective agar for effective isolation of C. difficile from stool specimens.

    abstract：BACKGROUND:ChromID Clostridium difficile agar (IDCd; bioMérieux SA, France) is a recently developed chromogenic medium for rapid and specific isolation of C. difficile. We compared the performance of IDCd with that of Clostridium difficile Selective Agar (CDSA). METHODS:A total of 530 fresh stool specimens were collec...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2014.34.1.15

    authors： Shin BM,Lee EJ

    更新日期：2014-01-01 00:00:00

  • Challenges and Considerations in Sequence Variant Interpretation for Mendelian Disorders.

    abstract：:In 2015, the American College of Medical Genetics and Genomics (ACMG), together with the Association for Molecular Pathology (AMP), published the latest guidelines for the interpretation of sequence variants, which have been widely adopted into clinical practice. Despite these standardized efforts, the degrees of subj...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章,评审

    doi：10.3343/alm.2019.39.5.421

    authors： Kim YE,Ki CS,Jang MA

    更新日期：2019-09-01 00:00:00

  • Application of Multigene Panel Sequencing in Patients with Prolonged Rate-corrected QT Interval and No Pathogenic Variants Detected in KCNQ1, KCNH2, and SCN5A.

    abstract：:Long QT syndrome (LQTS) is an inherited cardiac disease characterized by a prolonged heart rate-corrected QT (QTc) interval. We investigated the genetic causes in patients with prolonged QTc intervals who were negative for pathogenic variants in three major LQTS-related genes (KCNQ1, KCNH2, and SCN5A). Molecular genet...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2018.38.1.54

    authors： Seo SH,Kim SY,Cho SI,Park H,Lee S,Choi JM,Kim MJ,Lee JS,Ahn KJ,Song MK,Bae EJ,Park SS,Seong MW

    更新日期：2018-01-01 00:00:00

  • Evaluation of the QuantaMatrix Multiplexed Assay Platform for Molecular Diagnosis of Multidrug- and Extensively Drug-Resistant Tuberculosis Using Clinical Strains Isolated in Myanmar.

    abstract：BACKGROUND:Although the incidence of tuberculosis (TB) is decreasing, cases of multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB continue to increase. As conventional phenotype drug susceptibility testing (pDST) takes six to eight weeks, molecular assays are widely used to determine drug resistance. ...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2020.40.2.142

    authors： Chang Y,Kim S,Kim Y,Ei PW,Hwang D,Lee J,Chang CL,Lee H

    更新日期：2020-03-01 00:00:00

  • The role of cardiac biomarkers in the diagnosis and management of patients presenting with suspected acute coronary syndrome.

    abstract：:Myocardial infarction (MI) is the leading cause of death in the developed world. Biomarkers have an essential role in diagnosis, risk stratification, guiding management and clinical decision making in the setting of patients presenting with signs and symptoms of MI. Cardiac troponin (cTn) rose to prominence during the...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章,评审

    doi：10.3343/alm.2013.33.5.309

    authors： Christenson E,Christenson RH

    更新日期：2013-09-01 00:00:00

  • A case of IgM deficiency with B cell deficiency detected by ABO discrepancy in a patient with acute osteomyelitis.

    abstract：:ABO discrepancy refers to an inconsistency between red cell and serum typings and has various causes, including hypogammaglobulinemia. IgM deficiency is a rare disorder that may accompany several conditions such as infection and autoimmune disorders. Here, we describe a case of IgM deficiency discovered during the eva...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2013.33.3.208

    authors： Jung CL,Cha MK,Jun BH,Hong KS

    更新日期：2013-05-01 00:00:00

  • Galectin-3 Reflects the Echocardiographic Grades of Left Ventricular Diastolic Dysfunction.

    abstract：BACKGROUND:The level of Galectin-3 (Gal-3) protein purportedly reflects an ongoing cardiac fibrotic process and has been associated with ventricular remodeling, which is instrumental in the development of heart failure with preserved ejection fraction (HFpEF) syndrome. The aim of this study was to investigate the poten...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2018.38.4.306

    authors： Ansari U,Behnes M,Hoffmann J,Natale M,Fastner C,El-Battrawy I,Rusnak J,Kim SH,Lang S,Hoffmann U,Bertsch T,Borggrefe M,Akin I

    更新日期：2018-07-01 00:00:00

  • New decision criteria for selecting delta check methods based on the ratio of the delta difference to the width of the reference range can be generally applicable for each clinical chemistry test item.

    abstract：BACKGROUND:Many laboratories use 4 delta check methods: delta difference, delta percent change, rate difference, and rate percent change. However, guidelines regarding decision criteria for selecting delta check methods have not yet been provided. We present new decision criteria for selecting delta check methods for e...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2012.32.5.345

    authors： Park SH,Kim SY,Lee W,Chun S,Min WK

    更新日期：2012-09-01 00:00:00

  • Accessory Gene Regulator Polymorphism and Vancomycin Minimum Inhibitory Concentration in Methicillin-Resistant Staphylococcus aureus.

    abstract：BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) bacteremia with a vancomycin minimum inhibitory concentration (MIC) of 2 µg/mL presents a high rate of therapeutic failure in response to vancomycin. In addition, polymorphism in accessory gene regulator (agr) is associated with vancomycin therapeutic effect...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2015.35.4.399

    authors： Park MJ,Kim HS,Kim HS,Kim JS,Song W,Kim MY,Lee YK,Kang HJ

    更新日期：2015-07-01 00:00:00

  • Linezolid Resistance in Methicillin-Resistant Staphylococcus aureus in Korea: High Rate of False Resistance to Linezolid by the VITEK 2 System.

    abstract：:As various linezolid resistance mechanisms have been identified in methicillin-resistant Staphylococcus aureus (MRSA), we investigated the molecular characteristics of MRSA with elevated linezolid minimum inhibitory concentrations (MICs), using the VITEK 2 system (bioMérieux, Marcy-l'Étoile, France). Twenty-seven MRSA...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2020.40.1.57

    authors： Yoo IY,Kang OK,Shim HJ,Huh HJ,Lee NY

    更新日期：2020-01-01 00:00:00

  • Comparison of red blood cell hemolysis using plasma and serum separation tubes for outpatient specimens.

    abstract：BACKGROUND:To rapidly obtain outpatient results, we use plasma separation tubes (PST) for chemistry analysis. If lactate dehydrogenase measurement is required, serum separation tubes (SST) are used. There has been no evaluation of hemolysis with these tubes. We compared the hemolytic index (HI) obtained by using PST an...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2015.35.2.194

    authors： Ko DH,Won D,Jeong TD,Lee W,Chun S,Min WK

    更新日期：2015-03-01 00:00:00

  • Use of PCR with sequence-specific primers for high-resolution human leukocyte antigen typing of patients with narcolepsy.

    abstract：BACKGROUND:Narcolepsy is a neurologic disorder characterized by excessive daytime sleepiness, symptoms of abnormal rapid eye movement (REM) sleep, and a strong association with HLA-DRB1*1501, -DQA1*0102, and -DQB1*0602. Here, we investigated the clinico-physical characteristics of Korean patients with narcolepsy, their...

    journal_title：Annals of laboratory medicine

    pub_type： 杂志文章

    doi：10.3343/alm.2012.32.1.57

    authors： Woo HI,Joo EY,Hong SB,Lee KW,Kang ES

    更新日期：2012-01-01 00:00:00