Abstract:
:Synthesis of alpha- and beta-globin RNA in DMSO-induced Friend's erythroleukemia cells and synthesis of immunoglobulin gamma- and kappa-chain RNA, total RNA, 5S RNA, and tRNA in mouse myeloma cells (MPC-11) was inhibited by gamma-irradiation. For all RNA species, synthesis decreased nearly exponentially as a function of radiation dose, whereas RNA size distributions, turnover rates, and specific activities of radioactively labeled RNA were affected only insignificantly. D37 values for the loss of synthesis of various RNA species correspond to target sizes ranging from 21,000 to 53,000 kd, or 30-80 kbp of DNA. These target sizes are several-fold larger than the structural genes in question; however, they correspond well with the size of DNA loops, or "domains" constrained by the nuclear matrix. The data suggest that the eukaryotic transcription unit is the torsionally constrained chromatin loop, transcription of which may be inactivated, or significantly reduced by a DNA single-strand break.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Rodi CP,Sauerbier Wdoi
10.1002/jcp.1041410216subject
Has Abstractpub_date
1989-11-01 00:00:00pages
346-52issue
2eissn
0021-9541issn
1097-4652journal_volume
141pub_type
杂志文章abstract::Selective spermatozoa movement from storage of the oviduct to fertilization site is suggested to be a result of chemotaxis. In the present study, Natriuretic peptide precursor A (NPPA) induced sperm chemotaxis in capillaries and enhanced intracellular Ca(2+) level, both of which could be blocked by the Natriuretic Pep...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.22962
更新日期:2012-05-01 00:00:00
abstract::Although the role of several protooncogenes, including sis, myc, and myb in the regulation of growth and differentiation of vascular cells has been examined in some detail, limited information is available on the contribution of ras genes to these processes. In the present studies the influence of oncogenic ras transf...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041610312
更新日期:1994-12-01 00:00:00
abstract::Arterial smooth muscle cells undergo marked biochemical and morphological changes upon culturing. We have studied the time course of these changes in smooth muscle cells isolated from normal rabbit aortas by enzymic digestion and then maintained in Dulbecco's modified Eagle's medium with or without 10% rabbit serum. S...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041080321
更新日期:1981-09-01 00:00:00
abstract::Silibinin is the primary active compound in silymarin. It has been demonstrated to exert anti-carcinogenic effects and hepato-protective effects. However, the effects of silibinin on the maturation and immunostimulatory activities exhibited by dendritic cells (DCs) remain, for the most part, unknown. In this study, we...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20852
更新日期:2007-02-01 00:00:00
abstract::The carrier of uridine transport in hamster cells in culture is highly susceptible to the inhibitory effect of probes like S-benzylated derivatives of mercaptopurine nucleosides. The interaction between the probes and the carrier is competitive and reversible and it takes place at a site different from the substrate b...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040890451
更新日期:1976-12-01 00:00:00
abstract::Glioblastoma, the most common brain tumor, is characterized by high proliferation rate, invasion, angiogenesis, and chemo- and radio-resistance. One of most remarkable feature of glioblastoma is the switch toward a glycolytic energetic metabolism that leads to high glucose uptake and consumption and a strong productio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25421
更新日期:2017-02-01 00:00:00
abstract::Regulation of cellular Mg(2+) levels by insulin has been shown in various tissues. However, the mechanisms for hormonal regulation of cellular Mg(2+) have not been well described. We studied the effect of insulin on Na(+)/Mg(2+) exchange in normal human cells, measuring Na(+)/Mg(2+) exchange activity as net total Mg(2...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10463
更新日期:2004-06-01 00:00:00
abstract::Mice of the C3H/HeJ strain exhibit low inflammatory and immunological responses to certain bacterial lipopolysaccharide (LPS) preparations. Lymphocytes from C3H/HeJ mice also show defective LPS-induced mitogenesis. We tested the colony stimulating activity (CSA) response of C3H/HeJ mice. As controls we used mice of th...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040920219
更新日期:1977-08-01 00:00:00
abstract::This study examined the role of agonist-induced Rho kinase (ROCK) involvement in the morphological outcome of pulmonary-derived fibroblasts. Normal human lung fibroblasts (NHLF) spontaneously differentiate into network-like structures in a two-dimensional growth factor reduced Matrigel matrix-based assay. Sphingosine ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20939
更新日期:2007-05-01 00:00:00
abstract::Although it is well established that epidermal growth factor receptors (EGFRs) are asymmetrically expressed at the basolateral plasma membrane in polarized epithelial cells, how this process is regulated is not known. The purpose of this study was to address the mechanism of directed EGFR basolateral sorting using the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041620316
更新日期:1995-03-01 00:00:00
abstract::Multicolor immunostaining analysis is often a desirable tool in cell biology for most researchers. Nonetheless, this is not an easy task and often not affordable by many laboratories as it might require expensive instrumentation and sophisticated analysis software. Here, we describe a simple protocol for performing se...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24506
更新日期:2014-06-01 00:00:00
abstract::The secosteroid hormone 1alpha, 25-dihydroxyvitamin D3 (1,25D) has been shown to regulate the growth and differentiation of human prostate cancer (PCa) cells, although the precise molecular mechanisms mediating these effects have not been defined. Previous studies in our laboratory demonstrated that the antiproliferat...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20692
更新日期:2006-09-01 00:00:00
abstract::Repetitive mechanical stimulation of cultured avian skeletal muscle increases the synthesis of prostaglandins (PG) E2 and F2 alpha which regulate protein turnover rates and muscle cell growth. These stretch-induced PG increases are reduced in low extracellular calcium medium and by specific phospholipase inhibitors. M...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041550109
更新日期:1993-04-01 00:00:00
abstract::Retinoic acid (RA) reduced growth, fibronectin, and retinoic acid receptor (RAR alpha) in NIH 3T3 cells but not in cells transformed by the Ha-ras oncogene. RA lowered RAR alpha transcript and protein, increased RAR beta transcripts, and had no effect on RAR gamma. H-ras transformation downregulated RAR expression and...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199711)173:2<297::AID-JCP3
更新日期:1997-11-01 00:00:00
abstract::Podocyte injury is a critical factor for the initiation and progression of diabetic kidney disease (DKD). However, the underlying mechanisms of podocyte injury in DKD have not been completely elucidated. Studies suggested that intracellular cholesterol accumulation was correlated with podocyte injury, but the cause of...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28924
更新日期:2019-12-01 00:00:00
abstract::CHFR acts as a tumor suppressor gene, which is frequently inactivated caused by its promoter hypermethylation in various solid tumors. Although a recent study showed that CHFR hypermethylation was a frequent event in acute myeloid leukemia (AML) and correlated with adverse clinical outcome, herein, we found that CHFR ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26253
更新日期:2018-06-01 00:00:00
abstract::The SV40 transformed murine macrophage cell line, BAC1, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 132:1158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BAC1 were established. Clones r...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041300316
更新日期:1987-03-01 00:00:00
abstract::Tissue expansion, a technique to enlarge the skin surface area with an expandable balloon, has been widely used in reconstructive surgery. Although the effect of tissue expansion on in vivo skin physiology and histology has been well documented, it remains unclear whether keratinocytes or other cell types are responsi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199710)173:1<64::AID-JCP8>
更新日期:1997-10-01 00:00:00
abstract::Rat pheochromocytoma PC12 cells respond to the binding of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) by extending neurites in a manner resembling sympathetic neurons. This response requires cell attachment to an appropriate substratum (Fujii et al., J. Neurosci., 2:1157, 1982); attachment fact...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041380117
更新日期:1989-01-01 00:00:00
abstract::Peroxisome proliferator activated receptors (PPARs, alpha, beta/delta, gamma) control lipid homeostasis and differentiation in various tissues and tumor cells. PPARbeta and PPARgamma increase oligodendrocyte maturation in glial mixed populations and spinal cord oligodendrocytes, respectively, and PPARbeta is known to ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21509
更新日期:2008-11-01 00:00:00
abstract::The molecular weight of rat basic fibroblast growth factor is predicted to be 18 kDa when the amino acid sequence is read from the single AUG initiation codon found in the cDNA. DNA sequencing upstream of this AUG codon indicated, however, that there was an extended open reading frame. In vitro translation of the rat ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041480204
更新日期:1991-08-01 00:00:00
abstract::The poor prognosis of pancreatic cancer patients using currently available therapies mandates novel therapeutics that combine anti-neoplastic potency with toxicity-minimizing cancer specificity. Employing an overlapping pathway screen to identify genes exhibiting coordinated expression as a consequence of terminal cel...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21320
更新日期:2008-05-01 00:00:00
abstract::Biochemical events elicited by interleukin 1 (IL-1) were studied in Swiss 3T3 fibroblasts. One hour after its addition, IL-1 stimulated synthesis of prostaglandin E2 (PGE2), which continued to accumulate for 4 days. IL-1 also stimulated cAMP accumulation. Indomethacin blocked cAMP accumulation in response to IL-1, sug...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041390106
更新日期:1989-04-01 00:00:00
abstract::Gicerin/CD146 is a cell adhesion molecule, which belongs to the immunoglobulin (Ig) superfamily. We have reported that it has a homophilic binding activity, which participates in the neurite extension from embryonic neurons. To elucidate how gicerin is involved in the neurite extension mechanism, we employed PC12 cell...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20365
更新日期:2005-08-01 00:00:00
abstract::Mesenchymal stromal cells (MSCs) have been widely exploited for the treatment of several conditions due to their intrinsic regenerative and immunomodulatory properties. MSC have demonstrated to be particularly relevant for the treatment of ischemic diseases, where MSC-based therapies can stimulate angiogenesis and ind...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29622
更新日期:2020-10-01 00:00:00
abstract::Obese women offspring have a higher risk of developing chronic diseases associated with an altered immune function. We aim to determine, in neonatal monocyte-derived macrophages, whether maternal obesity is associated with an altered expression and DNA methylation of pro- and anti-inflammatory genes, along with a high...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25845
更新日期:2017-12-01 00:00:00
abstract::Chrysophanol is an anthraquinone compound, mainly isolated from rhubarb, with anti-cancer effects on some types of cancer cells. However, effects of chrysophanol on human choriocarcinoma cells are not known. Therefore, the objective of this study was to determine effects of chrysophanol on choriocarcinoma cells (JAR a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.25423
更新日期:2017-02-01 00:00:00
abstract::Transient receptor potential vanilloid 4 (TRPV4) channels are mechanosensitive ion channels that regulate systemic endothelial cell (EC) functions such as vasodilation, permeability, and angiogenesis. TRPV4 is expressed in retinal ganglion cells, Müller glia, pigment epithelium, microvascular ECs, and modulates cell v...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30116
更新日期:2020-10-20 00:00:00
abstract::In order to promote cell attachment, fibronectin must first undergo activation by a suitable substrate. In this study, 52 materials have been surveyed for their ability a) to bind fibronectin, b) to activate the cell-adhesive property of fibronectin, and c) to support the growth of cells. Many plastics, polysaccharide...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090314
更新日期:1981-12-01 00:00:00
abstract::We report the isolation of a bovine pituitary growth factor (MGF) for a rat mammary carcinoma cell line, 64-24, which was isolated from a highly hormone-dependent mammary tumor. The MGF has been partially purified by a series of Diaflo ultrafiltration membrane sievings, isoelectric focusing and Sephadex columns. The M...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1040930313
更新日期:1977-12-01 00:00:00