Abstract：
:Homer is a postsynaptic density (PSD) scaffold protein that is involved in synaptic plasticity, calcium signaling and neurological disorders. Here, we use pre-embedding immunogold electron microscopy to illustrate the differential localization of three Homer gene products (Homer 1, 2, and 3) in different regions of the mouse brain. In cross-sectioned PSDs, Homer occupies a layer ∼30-100nm from the postsynaptic membrane lying just beyond the dense material that defines the PSD core (∼30-nm-thick). Homer is evenly distributed within the PSD area along the lateral axis, but not at the peri-PSD locations within 60nm from the edge of the PSD, where type I-metabotropic glutamate receptors (mGluR1 and 5) are concentrated. This distribution of Homer matches that of Shank, another major PSD scaffold protein, but differs from those of other two major binding partners of Homer, type I mGluR and IP3 receptors. Many PSD proteins rapidly redistribute upon acute (2min) stimulation. To determine whether Homer distribution is affected by acute stimulation, we examined its distribution in dissociated hippocampal cultures under different conditions. Both the pattern and density of label for Homer 1, the isoform that is ubiquitous in hippocampus, remained unchanged under high K(+) depolarization (90mM for 2-5min), N-methyl-d-asparic acid (NMDA) treatment (50μM for 2min), and calcium-free conditions (EGTA at 1mM for 2min). In contrast, Shank and calcium/calmodulin-dependent kinase II (CaMKII) accumulate at the PSD upon NMDA treatment, and CaMKII is excluded from the PSD complex under low calcium conditions.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Tao-Cheng JH,Thein S,Yang Y,Reese TS,Gallant PEdoi
10.1016/j.neuroscience.2014.01.066subject
Has Abstractpub_date
2014-04-25 00:00:00pages
80-90eissn
0306-4522issn
1873-7544pii
S0306-4522(14)00096-7journal_volume
266pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:Previous investigations of cortical organization in the brain of the turtle have revealed many features typical of mammalian neocortex. Recent evidence suggests that many neocortical neurons contain neuroactive peptides. The possibility that one such peptide, somatostatin, is found in the turtle brain was tested using...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(83)90295-6
更新日期：1983-06-01 00:00:00
abstract：:In anaesthetized albino rabbits, the cerebellar flocculus was systematically mapped with a glass microelectrode to identify the location of Purkinje cells that inhibit specific vestibulo-ocular reflex pathways. The effects of microstimulation of the flocculus Purkinje cell layer on vestibular nerve-evoked reflexes to ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(82)90024-0
更新日期：1982-07-01 00:00:00
abstract：:The identification and characterization of excitatory and inhibitory neurons are significant steps in understanding neural network functions. In this study, we investigated the intrinsic electrophysiological properties of neurons in the prepositus hypoglossi nucleus (PHN), a brainstem structure that is involved in gaz...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.09.017
更新日期：2011-12-01 00:00:00
abstract：:Exercise induces neuroplasticity in descending motor pathways facilitating motor learning, and as such it could be utilized as an intervention in neurorehabilitation, for example when re-learning motor skills after stroke. To date, however, the neurophysiological and molecular mechanisms underlying exercise-induced ne...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.03.042
更新日期：2020-06-15 00:00:00
abstract：:It has previously been reported that the avian H5N1 type of influenza A virus can be detected in neurons and astrocytes of human brains in autopsy cases. However, the underlying neuropathogenicity remains unexplored. In this study, we used differentiated human astrocytic and neuronal cell lines as models to examine th...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.04.013
更新日期：2010-07-14 00:00:00
abstract：:Intracerebroventricular (i.c.v.) injection of kynurenic acid (KYNA) had sedative and hypnotic effects during stress in neonatal chicks. However, its mechanism has not been clarified. KYNA is an endogenous antagonist of the α7 nicotinic acetylcholine (α7nACh) receptor and N-methyl-d-aspartate (NMDA) receptor. Therefore...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2013.06.005
更新日期：2013-09-17 00:00:00
abstract：:Effective information transmission for open skill performance requires fine-scale coordination of distributed networks of brain regions linked by white matter tracts. However, how patterns of connectivity in these anatomical pathways may improve global efficiency remains unclear. In this study, we hypothesized that th...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.02.006
更新日期：2019-04-15 00:00:00
abstract：:Histamine H(3) autoreceptors induce a negative feedback on histamine synthesis and release. While it is known that cAMP/cAMP dependent protein kinase (PKA) and Ca(2+)/CaMKII transduction pathways mediate H(3) effects on histamine synthesis, the pathways regulating neuronal histamine release are poorly known. Given the...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.08.068
更新日期：2009-12-15 00:00:00
abstract：:X-linked forms of non-specific mental retardation are complex disorders, for which mutations in several genes have recently been identified. These include OPHN1, GDI1, PAK3, IL1RAPL, TM4SF2, FMR2 and RSK2. To investigate the mechanisms through which alterations of these gene products could result in cognitive impairme...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(02)00218-x
更新日期：2002-01-01 00:00:00
abstract：:It is well established that the supramammillary nucleus plays a critical role in hippocampal theta rhythm generation/regulation by its direct and indirect (via the septal complex) connections to the hippocampus. Previous morphological and electrophysiological studies indicate that both the supramammillo-hippocampal an...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00127-5
更新日期：2000-01-01 00:00:00
abstract：:We employed simultaneous field potential recordings from CA3, subiculum and entorhinal cortex in an in vitro brain slice preparation to understand the involvement of these limbic areas in the generation of the field potential oscillations that are induced by bath application of the muscarinic receptor agonist carbacho...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.02.021
更新日期：2017-04-21 00:00:00
abstract：:Pathological changes occur in areas of CNS tissue remote from inflammatory lesions in multiple sclerosis (MS) and its animal model experimental allergic encephalomyelitis (EAE). To determine if oxidative stress is a significant contributor to this non-inflammatory pathology, cortex tissues from mice with clinical sign...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.04.041
更新日期：2011-06-30 00:00:00
abstract：:Secretion of neurotrophins is critical for the delivery of neurotrophic support. Brain-derived neurotrophic factor is targeted to a regulated secretory pathway in neurons as well as the neurosecretory AtT-20 cells. Here, we show that pertussis toxin, which inactivates Gi and Go G proteins, inhibits up to 50% of the re...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00309-2
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:depression is characterized by a stable negative bias toward emotional stimuli. This bias is associated with abnormal activities in emotion-processing regions (such as the amygdala) and cognitive-control regions (such as the dorsolateral prefrontal cortex [DLPFC]). However, it remains unclear whether the emo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.10.053
更新日期：2012-01-10 00:00:00
abstract：:The basal forebrain cholinergic system is a critical component of the neurobiological substrates underlying attentional function. Orexin neurons are important for arousal and maintenance of wakefulness and are found in the area of the hypothalamus previously shown to project to the basal forebrain. We used dual-probe ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.09.050
更新日期：2005-01-01 00:00:00
abstract：:In recent years, there has been a great deal of progress toward understanding the role of the striatum and dopamine in action selection. The advent of new animal models and the development of optical techniques for imaging and stimulating select neuronal populations have provided the means by which identified synapses...
journal_title：Neuroscience
pub_type： 杂志文章,评审
doi：10.1016/j.neuroscience.2011.08.051
更新日期：2011-12-15 00:00:00
abstract：:Patients with dysphagia can have higher risks of aspiration after repetitive swallowing activity due to the "fatigue effect". However, it is still unknown how consecutive swallows affect brain activity. Therefore, we sought to investigate differences in swallowing brain networks formed during consecutive swallows usin...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.11.047
更新日期：2017-03-06 00:00:00
abstract：:While B2 receptors mediate pain and hyperalgesia induced by bradykinin, in normal rats, recent reports indicate that, in the setting of inflammation, B1 receptors also mediate pain and hyperalgesia. Since bradykinin-induced hyperalgesia in normal rats is mediated by prostaglandins released from the postganglionic symp...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00280-v
更新日期：1995-11-01 00:00:00
abstract：:The effects of treatment with the antidepressant drug imipramine on neuropeptide Y immunoreactivity were studied immunocytochemically in the rat brain cortex and hypothalamus. It was found that the level of neuropeptide Y immunoreactivity in the cortex was significantly lowered three and 24 h after the last dose of ch...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90366-v
更新日期：1991-01-01 00:00:00
abstract：:Maternal diabetes during pregnancy may increase the risk of neurodevelopmental disorders in the offspring by increasing inflammation. A major source of inflammatory signaling observed in diabetes is activation of the receptor for advanced glycation end-products (RAGE), and increased RAGE expression has been reported i...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.06.063
更新日期：2015-09-10 00:00:00
abstract：:Solid pieces of cerebellar primordia taken from 12-day-old C57BL embryos were implanted into the cerebellar parenchyma of 3- to 4-month-old "Purkinje cell degeneration" mutant mice and analysed 2-3 months later. Purkinje cell replacement was followed by means of immunocytochemistry with antisera against either cyclic ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(87)90002-9
更新日期：1987-01-01 00:00:00
abstract：:The aim of the present study was to determine whether thoracic spinal manipulation (SM) decreases temporal summation of back pain. The study comprised two controlled experiments including 16 and 15 healthy participants, respectively. Each study included six sessions during which painful or non-painful electrical stimu...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.03.006
更新日期：2017-05-04 00:00:00
abstract：:Locomotor stepping in the Nembutal-anesthetized rat was elicited by electrical stimulation of either of two sites in the right or left posterolateral hypothalamus. Essential midbrain loci were identified by reversibly blocking the elicited locomotion through local injections of the anesthetic procaine (15%, 0.5 microl...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(90)90251-x
更新日期：1990-01-01 00:00:00
abstract：:Perceiving the sensory consequences of our actions with a delay alters the interpretation of these afferent signals and impacts motor learning. For reaching movements, delayed visual feedback of hand position reduces the rate and extent of visuomotor adaptation, but substantial adaptation still occurs. Moreover, the d...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.07.041
更新日期：2020-10-15 00:00:00
abstract：:In vitro receptor autoradiography was used to localize, quantify and characterize [125I-Tyr8]bradykinin binding sites in all major spinal cord segments of normal rats and animals subjected to various chemical treatments and surgical lesions. [125I-Tyr8]bradykinin specific binding sites were predominantly located to su...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00161-b
更新日期：1995-10-01 00:00:00
abstract：:Experimental animal studies have demonstrated a number of receptor localizations on specific cortical afferents and neurons. The present study of human posterior cingulate cortex evaluates the laminar distributions of particular receptors and their likely association with components of the neuropil. Coverslip autoradi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(90)90359-c
更新日期：1990-01-01 00:00:00
abstract：:Chronic intermittent ethanol (CIE) exposure dysregulates glutamatergic and GABAergic neurotransmission, facilitating basolateral amygdala (BLA) pyramidal neuron hyperexcitability and the expression of anxiety during withdrawal. It is unknown whether ethanol-induced alterations in nucleus basalis magnocellularis (NBM) ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.12.014
更新日期：2020-12-29 00:00:00
abstract：:The postural, ocular motor, perceptive and neurovegetative syndromes resulting from unilateral vestibular neurectomy (UVN) symptoms could generate a stress and thereby activate the hypothalamo-pituitary-adrenal (HPA) axis. This study was aimed at determining whether UVN causes changes in the activity of the HPA axis, ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.02.070
更新日期：2009-06-02 00:00:00
abstract：:This study examined the localization and functional expression of ryanodine receptors (RyR) within the cochlea using a combination of reverse transcription-polymerase chain reaction, immunolabeling techniques, and confocal Ca2+ imaging. All three RyR isoform mRNA transcripts were detected in the adult rat cochlea. Imm...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2005.09.011
更新日期：2006-01-01 00:00:00
abstract：:Perceptual integration of sensory input from our two nostrils has received little attention in comparison to lateralized inputs for vision and hearing. Here, we investigated whether a binary odor mixture of eugenol and l-carvone (smells of cloves and caraway) would be perceived differently if presented as a mixture in...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2013.10.079
更新日期：2014-01-31 00:00:00
