Quantitative autoradiographic localization of [125I-Tyr8]bradykinin receptor binding sites in the rat spinal cord: effects of neonatal capsaicin, noradrenergic deafferentation, dorsal rhizotomy and peripheral axotomy.


:In vitro receptor autoradiography was used to localize, quantify and characterize [125I-Tyr8]bradykinin binding sites in all major spinal cord segments of normal rats and animals subjected to various chemical treatments and surgical lesions. [125I-Tyr8]bradykinin specific binding sites were predominantly located to superficial laminae of the rat dorsal horn, with the substantia gelatinosa showing the highest density of labelling (values ranging from 3.1 fmol/mg tissue in cervical to 4.5 fmol/mg tissue in lumbar segments). A moderate density (1.8-3.0 fmol/mg tissue) of specific binding was observed in lamina III, whereas in other areas, i.e. laminae I and IV-X, lower amounts of labelling were detected. Within the superficial laminae of the dorsal horn, [125I-Tyr8]bradykinin binding was largely distributed over the neurophil with some perikarya showing concentrations of labelling. In contrast, the ventral horn showed a rather homogeneous distribution of [125I-Tyr8]bradykinin binding over the neuropil, with silver grain alignments surrounding motoneuron perikaryas and proximal processes. Bradykinin, [Tyr8]bradykinin and B2 receptor antagonists (D-Arg[Hyp3,Thi5,D-Tic7,Oic8]bradykinin (Hoe 140), D-Arg[Tyr3,D-Phe7,Leu8]bradykinin, D-Arg[Hyp3, Leu8]bradykinin, D-Arg[Hyp2, Thi5,8,-Phe7]bradykinin D-Arg[Hyp3, D-Phe7, Leu8]bradykinin, Tyr0, D-Arg[Hyp3, D-Phe7, Leu8]bradykinin inhibited [125I-Tyr8]-bradykinin binding with very high subnanomolar affinities, while the B1 receptor agonist (Tyr0,des-Arg10-kallidin) and antagonist ([Leu8]-des-Arg9-bradykinin) did not significantly affect [125I-Tyr8]bradykinin binding at up to micromolar concentrations. Two weeks after unilateral lumbar dorsal rhizotomy (L1-L6) or peripheral lesions of the sciatic nerve, significant decreases ( +/- 50%) in [125I-Tyr8]bradykinin binding sites were found in ipsilateral laminae I-III of lumbar spinal cord.(ABSTRACT TRUNCATED AT 250 WORDS)






Lopes P,Kar S,Chrétien L,Regoli D,Quirion R,Couture R




Has Abstract


1995-10-01 00:00:00














  • Expression of pannexin1 in the CNS of adult mouse: cellular localization and effect of 4-aminopyridine-induced seizures.

    abstract::The expression pattern of pannexin1, a gene coding for a protein that forms gap junction channels, was studied as both mRNA and protein in the CNS of adult mouse. Pannexin1 was widely expressed in the CNS by neuronal cell types but not glial cells, except for Bergmann glial cells of the cerebellar cortex. Cells positi...


    pub_type: 杂志文章


    authors: Zappalà A,Cicero D,Serapide MF,Paz C,Catania MV,Falchi M,Parenti R,Pantò MR,La Delia F,Cicirata F

    更新日期:2006-08-11 00:00:00

  • Intact nociception-induced neuroplasticity in transgenic mice deficient in neuronal nitric oxide synthase.

    abstract::Nitric oxide is thought to play an important role in multiple forms of use-dependent synaptic plasticity, including that which follows noxious peripheral stimulation. This suggests that development of nociception-induced neuroplasticity should be impaired in transgenic animals lacking the neuronal form of nitric oxide...


    pub_type: 杂志文章


    authors: Crosby G,Marota JJ,Huang PL

    更新日期:1995-12-01 00:00:00

  • Effect of desipramine and fluoxetine on energy metabolism of cerebral mitochondria.

    abstract::Brain bioenergetic abnormalities in mood disorders were detected by neuroimaging in vivo studies in humans. Because of the increasing importance of mitochondrial pathogenetic hypothesis of Depression, in this study the effects of sub-chronic treatment (21days) with desipramine (15mg/kg) and fluoxetine (10mg/kg) were e...


    pub_type: 杂志文章


    authors: Villa RF,Ferrari F,Gorini A,Brunello N,Tascedda F

    更新日期:2016-08-25 00:00:00

  • Mechanisms of Neurological Dysfunction in GOSR2 Progressive Myoclonus Epilepsy, a Golgi SNAREopathy.

    abstract::Successive fusion events between transport vesicles and their target membranes mediate trafficking of secreted, membrane- and organelle-localised proteins. During the initial steps of this process, termed the secretory pathway, COPII vesicles bud from the endoplasmic reticulum (ER) and fuse with the cis-Golgi membrane...


    pub_type: 杂志文章,评审


    authors: Jepson JEC,Praschberger R,Krishnakumar SS

    更新日期:2019-11-10 00:00:00

  • Sphingolipid profiles are altered in prefrontal cortex of rats under acute hyperglycemia.

    abstract::Diabetes type 1 is a common autoimmune disease manifesting by insulin deficiency and hyperglycemia, which can lead to dementia-like brain dysfunctions. The factors triggering the pathological processes in hyperglycemic brain remain unknown. We reported in this study that brain areas with different susceptibility to di...


    pub_type: 杂志文章


    authors: Fiedorowicz A,Prokopiuk S,Zendzian-Piotrowska M,Chabowski A,Car H

    更新日期:2014-01-03 00:00:00

  • Metallothionein-I and -III expression in animal models of Alzheimer disease.

    abstract::Previous studies have described altered expression of metallothioneins (MTs) in neurodegenerative diseases like multiple sclerosis (MS), Down syndrome, and Alzheimer's disease (AD). In order to gain insight into the possible role of MTs in neurodegenerative processes and especially in human diseases, the use of animal...


    pub_type: 杂志文章


    authors: Carrasco J,Adlard P,Cotman C,Quintana A,Penkowa M,Xu F,Van Nostrand WE,Hidalgo J

    更新日期:2006-12-28 00:00:00

  • Muscarinic facilitation of the occurrence of depolarization-induced suppression of inhibition in rat hippocampus.

    abstract::Depolarization-induced suppression of inhibition is a transient decrease in GABAergic input to a hippocampal pyramidal cell following a brief depolarization of that cell. When recorded under whole-cell voltage clamp, monosynaptic, bicuculline-sensitive, GABA(A)-mediated currents are suppressed for a period lasting up ...


    pub_type: 杂志文章


    authors: Martin LA,Alger BE

    更新日期:1999-01-01 00:00:00

  • Endoplasmic reticulum (ER) stress protein responses in relation to spatio-temporal dynamics of astroglial responses to status epilepticus in rats.

    abstract::In the present study, we investigated whether endoplasmic reticulum (ER) stress is associated with neuronal- and astroglial-death in the hippocampus using LiCl-pilocarpine-induced status epilepticus (SE) rat model. Glucose-related protein (GRP) 78 and protein disulfide isomerase (PDI) expressions were transiently incr...


    pub_type: 杂志文章


    authors: Ko AR,Kim JY,Hyun HW,Kim JE

    更新日期:2015-10-29 00:00:00

  • 5-HT(1B) receptor regulation of serotonin (5-HT) release by endogenous 5-HT in the substantia nigra.

    abstract::Axonal release of serotonin (5-hydroxytryptamine, 5-HT) in the CNS is typically regulated by presynaptic 5-HT autoreceptors. Release of 5-HT in substantia nigra pars reticulata (SNr), a principal output from the basal ganglia, has seemed an interesting exception to this rule. The SNr receives one of the highest densit...


    pub_type: 杂志文章


    authors: Threlfell S,Greenfield SA,Cragg SJ

    更新日期:2010-01-13 00:00:00

  • Pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal polypeptide promote the genesis of calcium currents in differentiating mouse embryonic stem cells.

    abstract::Identification of novel molecules that can induce neuronal differentiation of embryonic stem (ES) cells is essential for deciphering the molecular mechanisms of early development and for exploring cell therapy approaches. Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide ...


    pub_type: 杂志文章


    authors: Chafai M,Basille M,Galas L,Rostene W,Gressens P,Vaudry H,Gonzalez BJ,Louiset E

    更新日期:2011-12-29 00:00:00

  • Chronic treatment with a classical neuroleptic alters excitatory amino acid and GABAergic neurotransmission in specific regions of the rat brain.

    abstract::The purpose of the following experiments was to describe some of the neurochemical changes that occur in the basal ganglia of rats exposed chronically to a classical neuroleptic, fluphenazine, and to relate these changes to extrapyramidal motor dysfunction. For these studies a combination of behavioural, receptor auto...


    pub_type: 杂志文章


    authors: Johnson AE,Liminga U,Lidén A,Lindefors N,Gunne LM,Wiesel FA

    更新日期:1994-12-01 00:00:00

  • Autoradiographic distribution of the D1 agonist [3H]SKF 38393, in the rat brain and spinal cord. Comparison with the distribution of D2 dopamine receptors.

    abstract::The regional distribution of the specific D1 agonist [3H]SKF 38393 (SKF 38393, 2,3,4,5-tetra-hydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine) has been studied autoradiographically in the rat CNS. The binding of [3H]SKF 38393 to striatal sections was saturable, stereospecific, reversible, of high affinity (Kd = 9.9 nM) a...


    pub_type: 杂志文章


    authors: Dubois A,Savasta M,Curet O,Scatton B

    更新日期:1986-09-01 00:00:00

  • Dual chemoarchitectonic lamination of the visual sector of the thalamic reticular nucleus.

    abstract::The chemoanatomical organization of the visual sector of the cat's thalamic reticular nucleus (TRN)-that is at the dorsal lateral geniculate nucleus (dLGN) and at the pulvinar nucleus (Pul)-was investigated with two novel cytoarchitectonic markers. The Wisteria floribunda agglutinin (WFA) binding reaction visualized t...


    pub_type: 杂志文章


    authors: Baldauf ZB

    更新日期:2010-02-03 00:00:00

  • Effects of ketamine anesthesia on central nociceptive processing in the rat: a 2-deoxyglucose study.

    abstract::Ketamine is a dissociative anesthetic with complex actions on the CNS. We investigated here the effects of ketamine anesthesia on somatosensory processing in the rat spinal cord, thalamus, and cerebral cortex, using the quantitative 2-deoxyglucose mapping technique. Unanesthetized or ketamine-anesthetized male Sprague...


    pub_type: 杂志文章


    authors: Porro CA,Cavazzuti M,Giuliani D,Vellani V,Lui F,Baraldi P

    更新日期:2004-01-01 00:00:00

  • Behavioral analysis of opiate-mediated inhibition in the early chick embryo.

    abstract::Morphine (opiate agonist) produced a dose-dependent decrease in the spontaneous motility of 5- and 9-day chick embryos. Naloxone (opiate antagonist) appeared to reverse competitively the inhibition of motility caused by morphine. The effects of morphine on spontaneous motility in 5-day embryos were also reversed stere...


    pub_type: 杂志文章


    authors: Maderdrut JL,Reitzel JL,Okado N,Oppenheim RW

    更新日期:1985-10-01 00:00:00

  • Role of the axodendritic tree in the functioning of helix bursting neurons: generation of pacemaker activity and propagation of action potentials along the axon.

    abstract::The purpose of this study was to determine the role of the axodendritic tree in the generation of bursting pacemaker activity in the identified Helix RPa1 neuron, which is homologous to the Aplysia R15 cell, and propagation of action potentials along the axons. In doing so, I used recording of RPa1 neuron electrical a...


    pub_type: 杂志文章


    authors: Kononenko NI

    更新日期:2000-01-01 00:00:00

  • The ionic channel of neural nicotinic acetylcholine receptors is funnel-shaped.

    abstract::Membrane currents evoked by iontophoretically applied acetylcholine were recorded from non-dissociated neurons of rat superior cervical ganglion using the whole-cell patch-clamp recording method. Blocking effects produced by a series of specially synthesized organic compounds, the blockers of the open channel of gangl...


    pub_type: 杂志文章


    authors: Skok VI,Voitenko SV,Kurenniy DE,Brovtsyna NB,Gmiro VE,Kertcer SL

    更新日期:1995-08-01 00:00:00

  • Capsaicin causes prolonged inhibition of voltage-activated calcium currents in adult rat dorsal root ganglion neurons in culture.

    abstract::The effect of capsaicin on voltage-activated calcium currents was investigated in voltage-clamped somata of cultured adult rat dorsal root ganglion neurons. About half the neurons studied were sensitive to capsaicin, which induced an inward current at negative membrane potentials accompanied by an increase in membrane...


    pub_type: 杂志文章


    authors: Docherty RJ,Robertson B,Bevan S

    更新日期:1991-01-01 00:00:00

  • A stain for ischaemic or excessively stimulated neurons.

    abstract::Loyez' myelin stain sometimes impregnates the perikarya and processes of neurons. This is rare in normal adult mice, but can be provoked by various means including electrical stimulation and mild ischaemia shortly before fixation. Passing a direct current or a train of pulses across the dura of the cerebral cortex pro...


    pub_type: 杂志文章


    authors: Clarke PG,Nussbaumer JC

    更新日期:1987-12-01 00:00:00

  • Differential effect of protein kinase inhibitors on calcium-dependent and calcium-independent [14C]GABA release from rat brain synaptosomes.

    abstract::Rat brain synaptosomes were isolated to study the effects of protein kinase inhibitors (sphingosine, 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride, N-(6-aminohexyl)-5-chloro-1-naphtalenesulfonamide, staurosporine) on Ca2+-dependent and Ca2+-independent [14C]GABA release. The Ca2+-dependent [14C]GABA re...


    pub_type: 杂志文章


    authors: Storchak LG,Pozdnyakova NG,Himmelreich NH

    更新日期:1998-08-01 00:00:00

  • Stimulation-mediated translocation of calmodulin and neurogranin from soma to dendrites of mouse hippocampal CA1 pyramidal neurons.

    abstract::Calmodulin (CaM) and neurogranin (Ng) are two abundant neuronal proteins in the forebrain whose interactions are implicated in the enhancement of synaptic plasticity. To gain further insight into the actions of these two proteins we investigated whether they co-localize in principle neurons and whether they respond to...


    pub_type: 杂志文章


    authors: Huang KP,Huang FL,Shetty PK

    更新日期:2011-03-31 00:00:00

  • Evidence that integrins contribute to multiple stages in the consolidation of long term potentiation in rat hippocampus.

    abstract::Three structurally distinct groups of antagonists were used to test the hypothesis that integrin adhesion receptors play an essential role in consolidating (stabilizing) long term potentiation of the Schaffer collaterals in rat hippocampus. Comparisons were made of percent potentiation at antagonist-treated versus con...


    pub_type: 杂志文章


    authors: Chun D,Gall CM,Bi X,Lynch G

    更新日期:2001-01-01 00:00:00

  • Region and context-specific intracellular responses associated with cocaine-induced conditioned place preference expression.

    abstract::The development and maintenance of cocaine addiction depend heavily on learned reward-environment associations that can induce drug-seeking behavior and relapse. Understanding the mechanisms underlying these cue-induced conditioned responses is important for relapse prevention. To test whether intracellular responses ...


    pub_type: 杂志文章


    authors: Nygard SK,Klambatsen A,Balouch B,Quinones-Jenab V,Jenab S

    更新日期:2015-02-26 00:00:00

  • The distribution of small and intermediate conductance calcium-activated potassium channels in the rat sensory nervous system.

    abstract::Small (SK) and intermediate (IK) conductance calcium-activated potassium channels are candidate ion channels for the regulation of excitability in nociceptive neurones. We have used unique peptide-directed antisera to describe the immunocytochemical distribution of the known isoforms of these ion channels in dorsal ro...


    pub_type: 杂志文章


    authors: Mongan LC,Hill MJ,Chen MX,Tate SN,Collins SD,Buckby L,Grubb BD

    更新日期:2005-01-01 00:00:00

  • Gray matter damage in multiple sclerosis: Impact on clinical symptoms.

    abstract::Traditionally, multiple sclerosis (MS) is considered to be a disease primarily affecting the white matter (WM). However, the development of some clinical symptoms such as cognitive impairment cannot be fully explained by the severity of WM pathology alone. During the past decades it became clear that gray matter (GM) ...


    pub_type: 杂志文章,评审


    authors: van Munster CE,Jonkman LE,Weinstein HC,Uitdehaag BM,Geurts JJ

    更新日期:2015-09-10 00:00:00

  • Dual Function of Primary Somatosensory Cortex in Cognitive Control of Language: Evidence from Resting State fMRI.

    abstract::Resting state functional connectivity can be leveraged to investigate bilingual individual differences in cognitive control of language; however, thus far no report is provided on how the connectivity profiles of brain functional networks at rest point to different language control behavior in bilinguals. In order to ...


    pub_type: 杂志文章


    authors: Tabassi Mofrad F,Jahn A,Schiller NO

    更新日期:2020-10-15 00:00:00

  • Chronic progesterone treatment of male rats with unilateral 6-hydroxydopamine lesion of the dorsal striatum exacerbates [corrected] parkinsonian symptoms.

    abstract::Progesterone (PROG) shows neuroprotective effects in numerous lesion models, including a mouse model of Parkinson's disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). However, the possible beneficial effects of PROG on the behavioral and neurochemical impairments incurred in the hemiparkinson...


    pub_type: 杂志文章


    authors: Chao OY,Huston JP,von Bothmer A,Pum ME

    更新日期:2011-11-24 00:00:00

  • Genetic deletion of MT₁/MT₂ melatonin receptors enhances murine cognitive and motor performance.

    abstract::Melatonin, an indoleamine hormone secreted into circulation at night primarily by the brain's pineal gland, has been shown to have a wide variety of actions on the development and physiology of neurons in the CNS. Acting via two G-protein-coupled membrane receptors (MT1 and MT2), melatonin modulates neurogenesis, syna...


    pub_type: 杂志文章


    authors: O'Neal-Moffitt G,Pilli J,Kumar SS,Olcese J

    更新日期:2014-09-26 00:00:00

  • Metabolic myopathy produced by acute inhibition of glyceraldehyde-3-phosphate dehydrogenase with ortho-iodosobenzoic acid.

    abstract::A previously developed model of exercise-induced muscle contracture using iodoacetate to inhibit glyceraldehyde-3-phosphate dehydrogenase in rat hindlimb muscles produced selective type II myofiber damage. Utilizing a modification of the same model system, rats were given intra-aortic ortho-iodosobenzoic acid (700 nmo...


    pub_type: 杂志文章


    authors: Locklear I,Feeback DL,Brumback RA

    更新日期:1990-01-01 00:00:00

  • Muscle resistance to slow ramp weakly depends on activation level.

    abstract::The mechanical response of human m. flexor pollicis longus to slow (3.2 degrees/s) linear stretch by 5.5 degrees was measured during sustained (45-60 s, 9-13.5 p.p.s.) unfused tetanus evoked by electrical stimulation. The stiffness increased during unfused tetanus. At the late phase of unfused tetanus it was 1.8 +/- 0...


    pub_type: 杂志文章


    authors: Gurfinkel VS,Ivanenko YP,Levik YS

    更新日期:1997-09-01 00:00:00