Quantitative autoradiographic localization of [125I-Tyr8]bradykinin receptor binding sites in the rat spinal cord: effects of neonatal capsaicin, noradrenergic deafferentation, dorsal rhizotomy and peripheral axotomy.


:In vitro receptor autoradiography was used to localize, quantify and characterize [125I-Tyr8]bradykinin binding sites in all major spinal cord segments of normal rats and animals subjected to various chemical treatments and surgical lesions. [125I-Tyr8]bradykinin specific binding sites were predominantly located to superficial laminae of the rat dorsal horn, with the substantia gelatinosa showing the highest density of labelling (values ranging from 3.1 fmol/mg tissue in cervical to 4.5 fmol/mg tissue in lumbar segments). A moderate density (1.8-3.0 fmol/mg tissue) of specific binding was observed in lamina III, whereas in other areas, i.e. laminae I and IV-X, lower amounts of labelling were detected. Within the superficial laminae of the dorsal horn, [125I-Tyr8]bradykinin binding was largely distributed over the neurophil with some perikarya showing concentrations of labelling. In contrast, the ventral horn showed a rather homogeneous distribution of [125I-Tyr8]bradykinin binding over the neuropil, with silver grain alignments surrounding motoneuron perikaryas and proximal processes. Bradykinin, [Tyr8]bradykinin and B2 receptor antagonists (D-Arg[Hyp3,Thi5,D-Tic7,Oic8]bradykinin (Hoe 140), D-Arg[Tyr3,D-Phe7,Leu8]bradykinin, D-Arg[Hyp3, Leu8]bradykinin, D-Arg[Hyp2, Thi5,8,-Phe7]bradykinin D-Arg[Hyp3, D-Phe7, Leu8]bradykinin, Tyr0, D-Arg[Hyp3, D-Phe7, Leu8]bradykinin inhibited [125I-Tyr8]-bradykinin binding with very high subnanomolar affinities, while the B1 receptor agonist (Tyr0,des-Arg10-kallidin) and antagonist ([Leu8]-des-Arg9-bradykinin) did not significantly affect [125I-Tyr8]bradykinin binding at up to micromolar concentrations. Two weeks after unilateral lumbar dorsal rhizotomy (L1-L6) or peripheral lesions of the sciatic nerve, significant decreases ( +/- 50%) in [125I-Tyr8]bradykinin binding sites were found in ipsilateral laminae I-III of lumbar spinal cord.(ABSTRACT TRUNCATED AT 250 WORDS)






Lopes P,Kar S,Chrétien L,Regoli D,Quirion R,Couture R




Has Abstract


1995-10-01 00:00:00














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    pub_type: 杂志文章


    authors: Herzog E,Gilchrist J,Gras C,Muzerelle A,Ravassard P,Giros B,Gaspar P,El Mestikawy S

    更新日期:2004-01-01 00:00:00

  • Amphetamine up-regulates activator of G-protein signaling 1 mRNA and protein levels in rat frontal cortex: the role of dopamine and glucocorticoid receptors.

    abstract::Acute and chronic exposure to psychostimulants results in altered function of G-protein-coupled receptors in the forebrain. It is believed that neuroadaptations in G-protein signaling contribute to behavioral sensitivity to psychostimulants that persists over a prolonged drug-free period. Proteins termed activators of...


    pub_type: 杂志文章


    authors: Schwendt M,McGinty JF

    更新日期:2010-06-16 00:00:00

  • HMGB1 binding heptamer peptide improves survival and ameliorates brain injury in rats after cardiac arrest and cardiopulmonary resuscitation.

    abstract::Excessive inflammatory response produced after cardiac arrest and cardiopulmonary resuscitation (CA/CPR) is one of major causes of cerebral injury. High mobility group box 1 (HMGB1) is a pro-inflammatory cytokine and its role in brain injury after CA/CPR is unclear. Herein we investigated whether blocking HMGB1 signal...


    pub_type: 杂志文章


    authors: Shi X,Li M,Huang K,Zhou S,Hu Y,Pan S,Gu Y

    更新日期:2017-09-30 00:00:00

  • Strand-specific RNA-sequencing analysis of multiple system atrophy brain transcriptome.

    abstract::Multiple system atrophy (MSA) is a sporadic neurodegenerative disease. The major pathological hallmark of MSA is the accumulation of α-synuclein in oligodendrocytes. In contrast to Parkinson's disease no definitive familial etiology for MSA has been determined. Yet, there is a growing body of evidence that perturbatio...


    pub_type: 杂志文章


    authors: Mills JD,Ward M,Kim WS,Halliday GM,Janitz M

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  • Midbrain pathways for prepulse inhibition and startle activation in rat.

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    pub_type: 杂志文章


    authors: Yeomans JS,Lee J,Yeomans MH,Steidl S,Li L

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  • The relationship of the medullary catecholamine containing neurones to the vagal motor nuclei.

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    pub_type: 杂志文章


    authors: Ritchie TC,Westlund KN,Bowker RM,Coulter JD,Leonard RB

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  • Neurotensin-containing projections from the retrosplenial cortex to the anterior ventral thalamic nucleus in the rat.

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    pub_type: 杂志文章


    authors: Fukami K,Kiyama H,Shiotani Y,Tohyama M

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  • Stereological estimates of dopaminergic, GABAergic and glutamatergic neurons in the ventral tegmental area, substantia nigra and retrorubral field in the rat.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Fernandez HL,Chen M,Nadelhaft I,Durr JA

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    pub_type: 杂志文章


    authors: Shi R,Luo J,Peasley M

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  • Inhibition of protein phosphatase 2A- and protein phosphatase 1-induced tau hyperphosphorylation and impairment of spatial memory retention in rats.

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 临床试验,杂志文章,随机对照试验


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  • Screening for Efficacious Anticonvulsants and Neuroprotectants in Delayed Treatment Models of Organophosphate-induced Status Epilepticus.

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    pub_type: 杂志文章


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  • The effect of neocortical lesions on the number of cells in neonatal or adult feline caudate nucleus: comparison to fetal lesions.

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    pub_type: 杂志文章


    authors: Loopuijt LD,Villablanca JR,Hovda DA,Huang E,Mancuso S

    更新日期:1997-03-01 00:00:00

  • Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain.

    abstract::Combining gene expression data with morphological information has revolutionized developmental neuroanatomy in the last decade. Visualization and interpretation of complex images have been crucial to these advances in our understanding of mechanisms underlying early brain development, as most developmental processes a...


    pub_type: 杂志文章


    authors: Lindsay S,Sarma S,Martínez-de-la-Torre M,Kerwin J,Scott M,Luis Ferran J,Baldock R,Puelles L

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  • Short-term plasticity in turtle dorsal horn neurons mediated by L-type Ca2+ channels.

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    pub_type: 杂志文章


    authors: Russo RE,Hounsgaard J

    更新日期:1994-07-01 00:00:00

  • Riluzole rescues cochlear sensory cells from acoustic trauma in the guinea-pig.

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    pub_type: 杂志文章


    authors: Wang J,Dib M,Lenoir M,Vago P,Eybalin M,Hameg A,Pujol R,Puel JL

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  • Electrophysiology and immunohistochemistry in the hippocampal ca1 and the dentate gyrus of rats chronically exposed to 1-bromopropane, a substitute for specific chlorofluorocarbons.

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    pub_type: 杂志文章


    authors: Fueta Y,Fukuda T,Ishidao T,Hori H

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    pub_type: 杂志文章


    authors: Legendre A,Courville J

    更新日期:1987-06-01 00:00:00

  • Depolarization by K+ and glutamate activates different neurotransmitter release mechanisms in GABAergic neurons: vesicular versus non-vesicular release of GABA.

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    pub_type: 杂志文章


    authors: Belhage B,Hansen GH,Schousboe A

    更新日期:1993-06-01 00:00:00

  • Occurrence and developmental pattern of neuromedin U-immunoreactive nerves in the gastrointestinal tract and brain of the rat.

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    pub_type: 杂志文章


    authors: Ballesta J,Carlei F,Bishop AE,Steel JH,Gibson SJ,Fahey M,Hennessey R,Domin J,Bloom SR,Polak JM

    更新日期:1988-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Amori L,Wu HQ,Marinozzi M,Pellicciari R,Guidetti P,Schwarcz R

    更新日期:2009-03-03 00:00:00

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    pub_type: 杂志文章


    authors: Corvaja N,Doucet G,Bolam JP

    更新日期:1993-07-01 00:00:00

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    pub_type: 杂志文章


    authors: Loughlin SE,Foote SL,Bloom FE

    更新日期:1986-06-01 00:00:00

  • Evidence of GABA-immunopositive neurons in the dorsal part of the lateral geniculate nucleus of reptiles: morphological correlates with interneurons.

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    pub_type: 杂志文章


    authors: Rio JP,Reperant J,Ward R,Miceli D,Medina M

    更新日期:1992-01-01 00:00:00