Abstract:
:Physiological studies suggest visceral spinal afferents are generally small diameter, unmyelinated C-fibers or myelinated Adelta-fibers, but little is known about the size and chemical phenotypes of visceral sensory neurons supplying the small intestine. This study examines the size and expression patterns of transient receptor potential vanilloid 1 (TRPV1), calcitonin gene-related peptide (CGRP), substance P (SP), neuronal nitric oxide synthase (NOS) and isolectin B4-binding (IB4) in dorsal root ganglion (DRG) neurons projecting to the gastrointestinal tract. The spinal afferent innervation of mouse jejunum and distal colon was investigated with retrograde neuronal tracing and multi-label immunohistochemistry. Expression of histochemical markers and soma sizes of retrogradely labeled DRG profiles were determined with confocal microscopy. Most (>75%) jejunal and colonic afferent neurons were medium- and large-sized cells. The majority (82%) of jejunal afferents expressed TRPV1, but few bound IB4. All retrogradely labeled jejunal afferents expressing NOS-immunoreactivity (64%) also expressed TRPV1 and CGRP and most expressed SP. Most labeled colonic afferents expressed TRPV1 (62%) and half expressed NOS. Taken together these data demonstrate that the spinal afferent supply of the jejunum and colon is largely from medium and large sensory neurons, suggesting most intestinal afferent axons are A fibers. The various chemically-defined subpopulations of afferents may play multiple roles in sensory innervation of the jejunum apart from nociceptive transduction. Additionally, we have identified a unique chemical code, TRPV1/NOS/CGRP/SP, that distinguishes many spinal afferent terminals from those of enteric neurons.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Tan LL,Bornstein JC,Anderson CRdoi
10.1016/j.neuroscience.2008.06.071subject
Has Abstractpub_date
2008-10-02 00:00:00pages
334-43issue
2eissn
0306-4522issn
1873-7544pii
S0306-4522(08)00989-5journal_volume
156pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::Localized distension of the intestine evokes an ascending excitatory reflex and a descending inhibitory reflex in the circular muscle layer. The sequential activation of these two reflexes is believed to underlie the motor pattern of peristalsis, which is responsible for the co-ordinated propulsion of intestinal conte...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(96)00040-1
更新日期:1996-07-01 00:00:00
abstract::Paired helical filaments isolated from brains of two different patients with Alzheimer's disease were extensively treated with the ionic detergent, sodium dodecyl sulphate. Filaments were solubilized at different extents, depending on the brain examined, thus suggesting the existence of two types of paired helical fil...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90440-d
更新日期:1992-09-01 00:00:00
abstract::The functional changes of astrocytes are deeply involved in neurodegenerating processes of various CNS diseases. ATP is released during various neuronal damages such as brain ischemia and may control astrocyte functions. We examined the effect of ATP on the production of nitric oxide in the cultured astrocytes from ra...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00804-7
更新日期:2003-01-01 00:00:00
abstract::Turning in circles is among the behaviors elicited by unilateral cholinergic stimulation of the substantia nigra. Recent studies have shown that microinjection of cholinergic agonists into the substantia nigra pars compacta increases dopamine release and turnover in the striatum of anesthetized rats [Hernández-López e...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)90378-6
更新日期:1994-09-01 00:00:00
abstract::Our recent data suggest that noradrenaline (NA) regulates expression of Per1 mRNA in rat C6 cells, as a model of brain astrocytes, by two distinct NA-mediating pathways. Although C6 cells possess potential astrocyte-type characteristics, we hypothesize that astrocytes located in a distinct tissue or organ play specifi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.04.024
更新日期:2011-06-30 00:00:00
abstract::Environmental factors during early life stages affect behavioral and physiological phenotypes in adulthood. We examined the effect of photoperiods during development on neurogenesis and affective behaviors during adolescence/adulthood using C57BL/6J mice. Mice were born and raised until weaning under long-day conditio...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2018.01.038
更新日期:2018-03-15 00:00:00
abstract::Experiments in a variety of preparations have indicated that excessive activation of receptors for the excitatory amino acids glutamate and aspartate may mediate irreversible anoxic neuronal injury. We investigated this hypothesis in the in vitro hippocampal slice. Rat hippocampal slices perfused for 40 min with buffe...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90027-3
更新日期:1987-06-01 00:00:00
abstract::Retinitis pigmentosa (RP) is a heterogeneous group of retinal degenerative diseases in which the final pathological feature is photoreceptor cell apoptosis. Currently, the pathogenesis of RP remains poorly understood and therapeutics are ineffective. 17β-Oestradiol (βE2) is universally acknowledged as a neuroprotectiv...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2020.11.010
更新日期:2021-01-01 00:00:00
abstract::It has been proposed that glutamatergic and dopaminergic systems are functionally opposed in their regulation of striatal output. The present study tested the effects of drugs that enhance AMPA-receptor-mediated glutamatergic transmission (ampakines) for their effects on dopamine-related alterations in cortical activi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00423-8
更新日期:2003-01-01 00:00:00
abstract::This study has investigated the role of hypothalamic and amygdalar type-1 cannabinoid (CB1) receptors in the emotional and neuroendocrine responses to stress. To do so, we used the Cre/loxP system to generate conditional mutant mice lacking the CB1 gene in neurons expressing the transcription factor single-minded 1 (S...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.08.049
更新日期:2012-03-01 00:00:00
abstract::High frequency stimulation (HFS) is applied to many brain regions to treat a variety of neurological disorders/diseases, yet the mechanism(s) underlying its effects remains unclear. While some studies showed that HFS inhibits the stimulated nucleus, others report excitation. In this in vitro study, we stimulated the r...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.06.055
更新日期:2007-10-12 00:00:00
abstract::In adult rats the mystacial vibrissae were clipped on one side of the snout, and the influence of this sensory deprivation on crossed and uncrossed striatal afferents from the substantia nigra, ventral tegmental area, and retrorubral area was examined with the horseradish peroxidase tract tracing technique. Unilateral...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90030-6
更新日期:1992-01-01 00:00:00
abstract::Sensory experience has a profound effect on neocortical neurons. Passive stimulation of whiskers or sensory deprivation from whiskers can induce long-lasting changes in neuronal responses or modify the receptive field in adult animals. We recorded barrel cortical neurons in urethane-anesthetized rats in layers 2/3 or ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.09.057
更新日期:2014-12-05 00:00:00
abstract::Acute stress is known to evoke a discrete pattern of c-fos expression in the brain. The work reported here shows that this pattern is modified in regionally specific ways following repeated stress, and that this can be correlated with changes in telemetered heart rate, core temperature and corticosterone output that o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00532-a
更新日期:1995-02-01 00:00:00
abstract::The glycine transporter GLYT2 is present in axonal boutons throughout the spinal cord, and its laminar distribution matches that of glycine-enriched axons, which are presumed to be glycinergic. In order to determine whether boutons which possess GLYT2 are glycine-enriched, we have carried out pre-embedding immunocytoc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00501-5
更新日期:1997-03-01 00:00:00
abstract::The existence of a sex difference in several chronic pain syndromes and the fluctuation of symptoms during the menstrual cycle strongly suggest sex hormones are involved in pain processing. The mechanisms underlying these changes are not well understood. Using the colorectal distention model in the rat, we previously ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2008.04.070
更新日期:2008-07-17 00:00:00
abstract::In rats anaesthetized with ketamine, rhythmic jaw-opening and jaw-closing movements were induced by palatal stimulation. The two masseter muscles (jaw-closing) and the four digastric muscles (jaw-opening) were fitted with electrodes, which could be used either for electrical stimulation or for recording electromyograp...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(86)90273-3
更新日期:1986-10-01 00:00:00
abstract::The uptake and transport of basic and acidic horseradish peroxidase isoenzymes was compared in the neuromuscular, visual and olfactory systems of Xenopus larvae and postmetamorphic frogs. The concentration (w/v) of the two preparations was corrected to compensate for their difference in enzymatic activity (unit/w), wh...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)92988-5
更新日期:1987-09-01 00:00:00
abstract::Repeated administration of amphetamine results in the well known phenomenon of reverse tolerance or sensitization. However, little is known about cellular and molecular mechanisms underlying acute versus chronic response to amphetamine. In this paper, we investigated the effects of acute (1.5 or 5 mg/kg) and chronic (...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(94)00537-f
更新日期:1995-04-01 00:00:00
abstract::Neurotrophic factors are known to promote neuronal survival during development and after acute brain injury. Recent data suggest that some neuropeptides also exhibit neurotrophic activities, as shown for the pituitary adenylate cyclase activating polypeptide, which increases the survival of various neuronal population...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00414-x
更新日期:1999-04-01 00:00:00
abstract::Altered gene expression mediated by calcium/calmodulin-dependent protein kinase II (CaMKII) and other intracellular signaling molecules plays an important role in activity-dependent neuroplasticity. We discovered that sustained depolarization induced by KCl, a commonly used paradigm for studying activity-dependent gen...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.06.041
更新日期:2009-09-29 00:00:00
abstract::High densities of mRNAs for three rhythm-related genes, rPeriod1 (rPer1), rPer2, and rClock, which share high homology in Drosophila and mice, were found in the hypothalamic suprachiasmatic nucleus (SCN). The SCN, however, is not the only brain region that expresses these genes. To understand the distributions and pos...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00004-6
更新日期:2003-01-01 00:00:00
abstract::An inflammatory response in the CNS mediated by activation of microglia is a key event in the early stages of the development of neurodegenerative diseases. Using mouse cortical mixed glia cultures, we have previously demonstrated that the bacterial endotoxin lipopolysaccharide induces the activation of microglia and ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(00)00057-9
更新日期:2000-01-01 00:00:00
abstract::The genetically epilepsy-prone rat is an animal model of inherited generalised tonic-clonic epilepsy that shows abnormal susceptibility to audiogenic seizures and a lowered threshold to a variety of seizure-inducing stimuli. Recent studies suggest a crucial role for glutamate and GABA transporters in epileptogenesis a...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00684-2
更新日期:1998-08-01 00:00:00
abstract::The present study was designed to investigate involvement of angiotensin II (Ang II) type 2 receptors (AT2 receptors) in restoration of perivascular nerve innervation injured by topical phenol treatment. Male Wistar rats underwent in vivo topical application of 10% phenol around the superior mesenteric artery. After p...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.09.026
更新日期:2007-12-12 00:00:00
abstract::Necker cube is one of the ambiguous figures that is physically a static image but can be alternately perceived in two different perspectives. A great deal of debate exists regarding ambiguous figures that induce spontaneous switching between rival percepts. To investigate the time course of neural processes underlying...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.04.015
更新日期:2014-06-20 00:00:00
abstract::Alterations in the phosphorylation state of the microtubule-associated protein tau have been associated with the pathogenesis of neurofibrillary degeneration as well as with a neuroprotective action against apoptotic cell death. Mitogen-activated protein kinases (MAPK) phosphorylate tau protein in vitro but the pathop...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00245-7
更新日期:2001-01-01 00:00:00
abstract::The hypocretin/orexin system regulates, among other things, sleep and energy homeostasis. The system is likely regulated by both homeostatic and circadian mechanisms. Little is known about local differences in the regulation of hypocretin activity. The aim of this study was to establish an optimized peptide quantifica...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.09.050
更新日期:2015-12-03 00:00:00
abstract::The ultrastructural features and synaptic contacts of two types of neurotensin-containing amacrine cells in turtle retina were examined by electron immunocytochemistry, and the retinal peptides were characterized using radioimmunoassay and high-pressure liquid chromatography. The two types of cell were distinguished o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(87)90146-1
更新日期:1987-05-01 00:00:00
abstract::Sensory-evoked propagating waves are frequently observed in sensory cortex. However, it is largely unknown how an evoked propagating wave affects the activity evoked by subsequent sensory inputs, or how two propagating waves interact when evoked by simultaneous sensory inputs. Using voltage-sensitive dye imaging, we i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2012.04.062
更新日期:2012-08-02 00:00:00