Abstract：
:The glycine transporter GLYT2 is present in axonal boutons throughout the spinal cord, and its laminar distribution matches that of glycine-enriched axons, which are presumed to be glycinergic. In order to determine whether boutons which possess GLYT2 are glycine-enriched, we have carried out pre-embedding immunocytochemistry with antibody raised against GLYT2, and combined this with post-embedding detection of glycine, in the rat. GLYT2 immunoreactivity was present in boutons which formed symmetrical axodendritic, axosomatic or axoaxonic synapses, and was often seen in peripheral axons of type II synaptic glomeruli. One hundred and fifty GLYT2-immunoreactive boutons were analysed quantitatively, and in 142 (94.6%) of these the density of gold particles representing glycine-like immunoreactivity exceeded the background level (over presumed glutamatergic boutons) by at least a factor of two. Within immunoreactive boutons, the GLYT2 reaction product was associated with the plasma membrane, but often appeared as discrete clumps and was generally excluded from the region of the active sites of synapses. These results confirm that GLYT2 is associated with glycine-enriched axonal boutons in the superficial dorsal horn. They also suggest that GLYT2 is unevenly distributed on the plasma membrane of these boutons, and raise the possibility that it may be excluded from synaptic clefts.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Spike RC,Watt C,Zafra F,Todd AJdoi
10.1016/s0306-4522(96)00501-5subject
Has Abstractpub_date
1997-03-01 00:00:00pages
543-51issue
2eissn
0306-4522issn
1873-7544pii
S0306452296005015journal_volume
77pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:To determine the role of the frontal cortex in the generalization of limbic seizures, we first produced unilateral cortical spreading depression to reversibly suppress neuronal activity in the motor cortex and then triggered an amygdala-kindled seizure. Three minutes following induction of unilateral spreading depress...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00656-3
更新日期：1999-01-01 00:00:00
abstract：:The development of mechanisms of neurotransmitter release is an important component in the formation of functional synaptic connections. Synaptic neurotransmitter release can be modulated by nitric oxide, a compound shown to have a variety of physiologic functions in the nervous system. The goal of this study was to d...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00152-8
更新日期：1997-10-01 00:00:00
abstract：:Six human fetuses of gestational ages 16-28 weeks were employed. The immunocytochemical avidin-biotin-peroxidase complex method combined with the silver Bodian technique was used to evaluate the presence of tyrosine hydroxylase and dopamine-beta-hydroxylase neurons and afferent and efferent fibres in the cerebellum du...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)00521-6
更新日期：1995-03-01 00:00:00
abstract：:Recent advances in multiple areas of research have contributed to the identification of several pathophysiological factors underlying obsessive-compulsive disorder (OCD). In particular, the glutamate transporter gene SLC1A1 has been associated with the diagnosis of OCD. Immunological and infectious studies have report...
journal_title：Neuroscience
pub_type： 杂志文章,评审
doi：10.1016/j.neuroscience.2009.10.043
更新日期：2010-01-20 00:00:00
abstract：:Inflammatory processes may play a critical role in the degeneration of basal forebrain cholinergic cells that underlies some of the cognitive impairments associated with Alzheimer's disease. In the present study, the proinflammagen lipopolysaccharide, from the cell wall of Gram-negative bacteria, was used to produce i...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00216-4
更新日期：1999-01-01 00:00:00
abstract：:Drinking alcohol during pregnancy is particularly detrimental for the developing brain and may cause a broad spectrum of cognitive and behavioral impairments, collectively known as fetal alcohol spectrum disorder (FASD). While behavioral abnormalities and brain damage have been widely investigated in animal models of ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.11.045
更新日期：2020-02-10 00:00:00
abstract：:In the present study, in vivo voltammetry was used to monitor changes in dopamine levels in the left and right medial prefrontal cortex of rats exposed to mild physical and psychological stress. These were 2 min of tail-pinch and 15 min exposure to cat odour, respectively. Fourteen male Long Evans rats with bilateral ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(97)00370-9
更新日期：1998-03-01 00:00:00
abstract：:The anatomical localization and pharmacology of alpha5 subunit-containing GABA type-A receptors in the human hippocampal formation of Alzheimer's disease patients were studied with an alpha5 receptor selective ligand, [3H]L-655,708 and compared to age-matched human controls. Autoradiographic analyses revealed a hetero...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00163-9
更新日期：2000-01-01 00:00:00
abstract：:The distribution of atrial natriuretic factor-like immunoreactivity in the central nervous system of the frog Rana ridibunda was investigated by indirect immunofluorescence and the immunogold technique, using an antiserum generated in rabbits against synthetic atrial natriuretic factor (Arg 101-Tyr 126). A stereotaxic...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(87)90225-9
更新日期：1987-07-01 00:00:00
abstract：:Previous studies have shown that when peripheral nerves in axolotl limbs are cut and surgically misdirected, regenerating axons grow back to the original pathways and innervate their correct muscles. In the present study however, we demonstrate that when given a choice between their correct nerve stump and an incorrec...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)00601-z
更新日期：1995-05-01 00:00:00
abstract：:The entorhinal cortex and other hippocampal and parahippocampal cortices are interconnected by a small number of GABAergic nonpyramidal neurons in addition to glutamatergic pyramidal cells. Since the cortical and basolateral amygdalar nuclei have cortex-like cell types and have robust projections to the entorhinal cor...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.01.028
更新日期：2015-04-02 00:00:00
abstract：:The possible irreversible blockade of 5-hydroxytryptamine1 receptor subtypes 5-hydroxytryptamine1A, 5-hydroxytryptamine1B/5-hydroxytryptamine1D and 5-hydroxytryptamine1C by the chloramine 8-methoxy-2-(N-2'-chloropropyl,N-propyl)aminotetralin (8-MeO-2'-chloro-PAT) was investigated in rat brain sections by quantitative ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(89)90436-3
更新日期：1989-01-01 00:00:00
abstract：:The effects of two benzodiazepines, diazepam and triazolam, on long-term potentiation were tested in slices of hippocampus and piriform cortex. The drugs had little influence on baseline synaptic responses but both were very effective in blocking LTP elicited by theta pattern stimulation. The effects were fully revers...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(92)90071-9
更新日期：1992-07-01 00:00:00
abstract：:Glutamic acid decarboxylase and GABA immunoreactivities were qualitatively and quantitatively evaluated in the cerebellar cortex of adult rats prenatally exposed to a low concentration of carbon monoxide (75 parts per million). Carbon monoxide-exposed and control rats were perfused with modified Bouin's fluid and thei...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2005.06.058
更新日期：2005-01-01 00:00:00
abstract：:Aged rats may be behaviorally classified as either cognitively impaired or unimpaired based upon their performance in the Morris water maze task. In aged Long-Evans rats, emergence of functional deficits has been related to the increase in the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor s...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00459-0
更新日期：2000-01-01 00:00:00
abstract：:Mice provide a highly valuable resource for investigating learning and memory processes; however, many of the established tasks for evaluating learning and memory were developed for rats. Behaviors of mice in these tasks appear to be driven by different motivational factors, and as a result, they often do not perform ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.12.029
更新日期：2010-03-10 00:00:00
abstract：:The importance and diversity of calcium signaling in the brain is mirrored by the expression of a multitude of voltage-activated calcium channel (Ca(V)) isoforms. Whereas the overall distributions of alpha(1) subunits are well established, the expression patterns of distinct channel isoforms in specific brain regions ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.02.037
更新日期：2010-05-19 00:00:00
abstract：:Brainstem projections to hypoglossal motoneurons innervating the intrinsic and extrinsic muscles of the tongue were determined using the transneuronal transfer of Herpes simplex virus-1. Injections of Herpes simplex virus-1 into the intrinsic muscles of the anterior tongue, the geniohyoid and styloglossus muscles each...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00203-u
更新日期：1995-10-01 00:00:00
abstract：:The postnatal development of GABAB binding sites in rat brain was studied by quantitative receptor autoradiography using [3H]GABA under selective conditions. Binding levels peak at regionally specific times during the first three weeks of life and then decline to adult levels. GABAB binding peaked in the globus pallid...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(94)90392-1
更新日期：1994-09-01 00:00:00
abstract：:Basic fibroblast growth factor is expressed in different isoforms which display tissue and species specificity and are differentially regulated during development and after experimental interventions. The differential regulation of the fibroblast growth factor-2 isoforms may indicate specific activities and functions ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00247-5
更新日期：2000-01-01 00:00:00
abstract：:The fenestrated microvasculature of the area postrema shows a less restrictive blood-brain barrier than is found in other areas of the CNS. We have studied the expression and relationship of vascular endothelial tight junctional proteins, astrocytes, macrophages, and the extracellular matrix with the extravasation of ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2007.09.023
更新日期：2007-12-05 00:00:00
abstract：:Several lines of evidence suggest that integrin receptors play a pivotal role in consolidation of long-term potentiation (LTP), but which of the many integrin dimers are involved remains to be discovered. The present study used an LTP reversal paradigm to test if alpha3 integrins make an important contribution. Functi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(01)00540-1
更新日期：2002-01-01 00:00:00
abstract：:The caudal anterior cingulate cortex (cAC) is an essential component of the circuitry involved in acquisition of forebrain-dependent trace eyeblink conditioning. Lesions of the cAC prevent trace eyeblink conditioning [Weible AP, McEchron MD, Disterhoft JF (2000) Cortical involvement in acquisition and extinction of tr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.11.046
更新日期：2007-03-02 00:00:00
abstract：:The modulation of the firing discharge of medial septal neurons and of the hippocampal electroencephalogram (EEG) mediated by actions on alpha2-adrenoreceptors (ARs) was investigated in awake rabbits. Bilateral i.c.v. infusion of a relatively low dose (0.5 microg) of the alpha2-AR agonist clonidine produced a reductio...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(03)00331-2
更新日期：2003-01-01 00:00:00
abstract：:The present study was designed to assess the possibility that sub-total ventral hippocampal lesions might leave intact a mechanism for only highly accurate navigation, whereas sub-total dorsal hippocampal lesions might leave intact a mechanism only for less precise navigation. Animals with selective dorsal, ventral or...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.05.007
更新日期：2004-01-01 00:00:00
abstract：:Physiological and anatomical investigations are commonly combined in experimental models. When studying the lower urinary tract (LUT), it is often of interest to perform both urodynamic studies and retrogradely labeled neurons innervating the peripheral target organs. However, it is not known whether the use of anatom...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2009.11.037
更新日期：2010-03-10 00:00:00
abstract：:The localization of glycine receptors in the rat brain was examined by means of in situ hybridization histochemistry using an oligonucleotide probe to the sequence of the alpha 1 subunit. Strongly- or moderately-labeled neurons were found in the cranial nuclei, sensory nuclei such as the spinal trigeminal nucleus, pri...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90302-5
更新日期：1991-01-01 00:00:00
abstract：:Postoperative cognitive dysfunction (POCD) is an important complication following major surgery and general anesthesia in older patients. However, the etiology of POCD remains largely to be determined. It is unknown how surgical stress and psychological stress affect the postoperative learning and memory function in g...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.02.015
更新日期：2016-04-21 00:00:00
abstract：:A new mammalian neuromuscular preparation is introduced for physiology and microscopy of all sorts: the intrinsic muscle of the mouse ear. The great utility of this preparation is demonstrated by illustrating how it has permitted us to develop a wholly new technique for staining muscle T-tubules, the critical conducti...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2019.07.028
更新日期：2020-07-15 00:00:00
abstract：:In this study, the nonlinear prediction method combined with Gaussian-scaled surrogate data was used to quantify, as a first goal, the chaotic behavior of the interspike interval of ventral tegmental area dopaminergic neurons, extracellularly recorded in vivo, in anesthetized rats. The second goal was to determine the...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00702-7
更新日期：1999-01-01 00:00:00
