Abstract:
:Apoptosis contributes greatly to the morphological and biochemical features of cell death after neonatal cerebral hypoxia-ischemia (HI), making this mode of cell death a promising therapeutic target. We previously showed that 10 mg/kg of the caspase inhibitor Q-VD-OPh at the onset of and immediately after HI on postnatal day 9 reduced brain infarct volume. In this study, delayed administration of Q-VD-OPh, 12 and 36 h after HI, decreased HI-induced caspase-3 activity (DEVD cleavage) by 23% and diminished the levels of the proinflammatory chemokines CCL2 (MCP-1) and CCL3 (MIP-1α) by 29.3 and 29.1%, respectively, but not the levels of the anti-inflammatory cytokines IL-4 and IL-10. Long-term administration of Q-VD-OPh initiated at 12 h after HI, and continued at 24-hour intervals for 2 weeks, reduced total brain tissue loss by 31.3% from 41.5±3.1 mm3 in the vehicle group to 28.5±3.0 mm3 in the Q-VD-OPh group when evaluated 16 weeks after HI (p=0.004). Q-VD-OPh treatment also ameliorated the loss of sensorimotor function, as evaluated by a cylinder rearing test (Q-VD-OPh: 30.8±4.3% vs. vehicle: 59.7±6.3% in nonimpaired forepaw preference) 3 weeks after HI, and reduced HI-induced hyperactivity, as measured in an open field test (Q-VD-OPh: 4,062±198 cm vs. vehicle: 4,792±205 cm in distance moved) 7 weeks after the insult. However, the functional protection was no longer observed when analyzed again at later time points. The mechanisms underlying the discrepancy between sustained morphological protection and transient functional protection remain to be elucidated.
journal_name
Dev Neuroscijournal_title
Developmental neuroscienceauthors
Han W,Sun Y,Wang X,Zhu C,Blomgren Kdoi
10.1159/000357939subject
Has Abstractpub_date
2014-01-01 00:00:00pages
64-72issue
1eissn
0378-5866issn
1421-9859pii
000357939journal_volume
36pub_type
杂志文章abstract::The metabolism of [1, 2-13C2] acetate via the tricarboxylic acid (TCA) cycle leads to the formation of a number of key mass isotopomers of glutamate. The distribution of these isotopomers which is a function of pyruvate carboxylase, pyruvate dehydrogenase and pyruvate recycling was used to determine the relative anapl...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111442
更新日期:1996-01-01 00:00:00
abstract::The spontaneously immortalized S16 Schwann cell line expresses higher levels of myelin-associated glycoprotein (MAG) and PO glycoprotein and their messenger RNAs when grown at high density than at low density [Sasagasako et al: J Neurochem 1996;66:1432-1439]. This up-regulation of myelin protein expression at high den...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017409
更新日期:1999-01-01 00:00:00
abstract::Insulin binding was measured on crude mitochondrial or plasma membranes prepared from different rat brain regions during postnatal development. In the cerebral cortex and brainstem, insulin binding decreased 60-70% between birth and the adult period. In the cerebellum, insulin binding doubled in the first 10 postnatal...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111863
更新日期:1990-01-01 00:00:00
abstract::A study was made on the development of cortical synapses in the gracile nucleus of rats using degeneration methods. A total of 46 animals, 1 adult and 45 neonates whose ages varied from 1 to 7 days, had the right somatosensory motor cortex destroyed. The survival period varied from 1 to 30 days. Identification of axon...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112118
更新日期:1994-01-01 00:00:00
abstract::Although cortical malformations (CMs) are often associated with epilepsy, the underlying mechanisms are unknown. The reeler mouse is a model of CM with enhanced susceptibility to epileptiform activity, including the in vitro dentate gyrus, a region normally resistant to seizures. In this study, field potential recordi...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000096212
更新日期:2007-01-01 00:00:00
abstract::Extracts prepared from embryonic, neonatal and adult rat brain were examined for the presence of oligodendroglial mitogens. Brain-derived mitogenic activities were found in all developmental stages with specific activity increasing during neonatal development. Extracts from postnatal day 7 contained the highest specif...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112301
更新日期:1985-01-01 00:00:00
abstract::Neuron-specific enolase (NSE) is a unique form of the glycolytic enzyme, enolase, which, in the brain, is strictly localized in differentiated neurons and neuroendocrine cells. In this report evidence is presented that NSE is first seen in the cell bodies of developing chick auditory and vestibular neurons shortly aft...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112689
更新日期:1982-01-01 00:00:00
abstract::Preadolescent animals display protracted hormonal stress responses mediated by the hypothalamic-pituitary-adrenal (HPA) axis compared to adults. Though the mechanisms that underlie this shift in stress reactivity are unknown, reduced glucocorticoid-dependent negative feedback on the HPA axis has been posited to contri...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000362873
更新日期:2014-01-01 00:00:00
abstract::The development of the chick bulbar cardiac ganglia has been studied ultrastructurally from the 3rd day of incubation to hatching. For descriptive purposes, their development has been divided into three phases. The first phase includes migration and aggregation of undifferentiated neuroblasts, stages 21-26 (day 3.5-5)...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112390
更新日期:1980-01-01 00:00:00
abstract::Traumatic brain injury (TBI) is the leading cause of death and disability in children. Evidence-based guidelines for the management of this population are available; however, the data highlight significant deficiencies with few treatment standards or guidelines. Considering the limited availability of resources, it is...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000094153
更新日期:2006-01-01 00:00:00
abstract::Recent studies have shown a protection from cerebral hypoxic-ischemic (HI) brain damage in the immature rat following a prior systemic hypoxic exposure when compared with those not exposed previously. To investigate the mechanism(s) of hypoxic preconditioning, brain glycogen and high-energy phosphate reserves were mea...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000069051
更新日期:2002-01-01 00:00:00
abstract::Central nervous system (CNS) stem cells require epidermal growth factor (EGF) to survive and express glial-specific proteins (GSPs) under its influence. EGF and its receptor (EGF-R) therefore are involved in gliogenesis. We hypothesize that EGF selectively modulates GSP expression in pluripotential CNS cells and this ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000048699
更新日期:2001-01-01 00:00:00
abstract::A total of 216 male and female albino Wistar rats (Rattus norvegicus) between 10 and 60 days old from 42 litters were studied to determine mast cell characteristics within the thalamus. Whereas the mean numbers of mast cells within both the parenchyma and leptomeninges (combined) did not change appreciably after 15 da...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000112759
更新日期:1981-01-01 00:00:00
abstract::The L-type calcium channel activator +/-Bay K 8644 has recently been shown to provoke self-injurious biting in young mice. Since the serotonergic systems have been implicated in the expression of self-injurious behavior in both humans and animals, the present studies tested whether drugs influencing serotonin systems ...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000066747
更新日期:2002-01-01 00:00:00
abstract::Proper eye formation depends on specific interactions between neural and ectodermal tissues coupled with temporally distinct gene expression and a regulated sequence of signaling events. The homeobox gene Pax6 is vitally important to the entire process of eye development in both vertebrates and invertebrates. Pax6 exp...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000082273
更新日期:2004-01-01 00:00:00
abstract::In the era of therapeutic hypothermia, reliable preclinical studies are integral to successfully identify neuroprotective strategies to further improve outcomes of encephalopathy at term. We reviewed preclinical neuroprotection studies reported between January 2014 and June 2016 to assess the use of effective temperat...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000452859
更新日期:2017-01-01 00:00:00
abstract::Nogo is a member of the reticulon family. Our understanding of the physiological functions of the Nogo-A protein has grown over the last few years, and this molecule is now recognized as one of the most important axonal regrowth inhibitors present in central nervous system (CNS) myelin. Nogo-A plays other important ro...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000343143
更新日期:2012-01-01 00:00:00
abstract::Prenatal cocaine exposure results in behavioral abnormalities throughout development in rats, but little is known regarding the biological mechanisms underlying these abnormalities. Pregnant rats received subcutaneous twice-daily injections (1 ml/kg) of normal saline or 15 mg/kg of cocaine hydrochloride throughout ges...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000069363
更新日期:2002-01-01 00:00:00
abstract::Cortical dysplasia (CD) is now recognized as one of the major causes of pediatric focal neocortical epilepsy, and surgical procedures have been considered early in life. However, the mechanisms involved in seizure generation and intractability in these patients are still unknown. We analyzed with immunocytochemistry f...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017406
更新日期:1999-11-01 00:00:00
abstract::Pediatric traumatic brain injury is a significant problem that affects many children each year. Progress is being made in developing neuroprotective strategies to combat these injuries. However, investigators are a long way from therapies to fully preserve injured neurons and glia. To restore neurological function, re...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000367784
更新日期:2015-01-01 00:00:00
abstract::Neuroprotective cold-shock proteins (CSPs) are abundant in the normothermic neonatal rodent brain but decrease with advancing neurodevelopmental age and are low or absent in the adult brain. It has not been established if neurodevelopmental age alters the baseline expression of CSPs in the human brain. Here, we tested...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000493637
更新日期:2018-01-01 00:00:00
abstract::Primary cultures of cerebellum from 5-day-old rats indicated that proliferating neuroblasts synthesize and release nerve growth factor (NGF). Since NGF promotes DNA synthesis in these cells, our findings demonstrate that the early developing cerebellum is a suitable physiological model for studying the autocrine mitog...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111673
更新日期:1992-01-01 00:00:00
abstract::The cytoskeleton of a newly isolated mouse embryo brain-derived cell line and of dissected mesencephalon-rhombencephalon samples of the 10- to 11-day-old mouse embryo, constituted of a ventricular zone only, has been examined by immunocytochemistry, electrophoretic separation and Western blotting techniques. In accord...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111612
更新日期:1987-01-01 00:00:00
abstract::Autism spectrum disorder (ASD) comprises a group of neurodevelopmental disorders characterized by social deficits and stereotyped behaviors. While several theories have emerged, the pathogenesis of ASD remains unknown. Although studies report dopamine signaling abnormalities in autistic patients, a coherent dopamine h...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000478725
更新日期:2017-01-01 00:00:00
abstract::The medial frontal cortex (MFC) is involved in the temporal organization of behaviour. It receives timing information from the master circadian clock in the suprachiasmatic nucleus (SCN), and exhibits daily oscillations in gene expression itself. In this study, we evaluate various properties of circadian rhythms of lo...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000232559
更新日期:2009-01-01 00:00:00
abstract::Glutamine synthesis, the major pathway of ammonia detoxification, and the intracellular concentration of organic osmolytes in primary astrocytes and F98 glioma cells were investigated with multinuclear magnetic resonance spectroscopy. Acute exposure to ammonia (3 h incubation with NH4Cl) raised the concentration of gl...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017339
更新日期:1998-01-01 00:00:00
abstract::There is an important unmet need to further improve the outcome of neonatal encephalopathy in term infants. Meta-analyses of large controlled trials now suggest that maternal magnesium sulfate (MgSO4) therapy is associated with a reduced risk of cerebral palsy and gross motor dysfunction after premature birth, but tha...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000362206
更新日期:2014-01-01 00:00:00
abstract::We have used whole-cell and single-channel recording techniques to investigate the electrophysiological properties of cultured ovine oligodendrocytes (OLGs). Our studies have led to the following conclusions. (1) Cultured mature OLGs express a variety of voltage-dependent K+ conductances including an outward current t...
journal_title:Developmental neuroscience
pub_type: 杂志文章,评审
doi:10.1159/000111893
更新日期:1989-01-01 00:00:00
abstract::A glia-derived neurite-promoting factor has been purified from medium conditioned by C6 rat glioma cells. It induces neurite outgrowth in cultured mouse neuroblastoma cells and inhibits granule cell migration in explants of mouse cerebellum. This factor is a potent serine protease inhibitor which has recently been sho...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000111951
更新日期:1988-01-01 00:00:00
abstract::This study investigated the time of expression of two endothelial cell adhesion molecules, ICAM-1/CD54 and PECAM-1/CD31 in the developing postpartum mouse blood-brain barrier (BBB). Immunoultrastructural studies demonstrated that both adhesion molecules are initially expressed primarily on the luminal endothelial cell...
journal_title:Developmental neuroscience
pub_type: 杂志文章
doi:10.1159/000017352
更新日期:1998-01-01 00:00:00