Abstract:
:Bacillus anthracis, the causative agent of anthrax, relies on multiple virulence factors to subvert the host immune defense. Using Caenorhabditis elegans as an infection model, we screened approximately 5,000 transposon mutants of B. anthracis Sterne for decreased virulence. One of the attenuated mutants resulted in loss of expression of yceG and yceH, the last two genes in a six-gene cluster of tellurite resistance genes. We generated an analogous insertional mutant to confirm the phenotype and characterize the role of yceGH in resistance to host defenses. Loss of yceGH rendered the mutants more sensitive to tellurite toxicity as well as to host defenses such as reactive oxygen species and the cathelicidin family of antimicrobial peptides. Additionally, we see decreased survival in mammalian models of infection, including human whole blood and in mice. We identify a novel role for the yceGH genes in B. anthracis Sterne virulence and suggest that C. elegans is a useful infection model to study anthrax pathogenesis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Franks SE,Ebrahimi C,Hollands A,Okumura CY,Aroian RV,Nizet V,McGillivray SMdoi
10.1128/IAI.01614-13subject
Has Abstractpub_date
2014-03-01 00:00:00pages
1132-40issue
3eissn
0019-9567issn
1098-5522pii
IAI.01614-13journal_volume
82pub_type
杂志文章abstract::Nasopharyngeal colonization is a necessary first step in the pathogenesis of Streptococcus pneumoniae. Using isolates containing defined mutations in the S. pneumoniae capsule locus, we found that expression of the capsular polysaccharide is essential for colonization by the type 2 strain D39 and the type 3 strains A6...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3755-3761.2001
更新日期:2001-06-01 00:00:00
abstract::The smooth lipopolysaccharide complex of the outer surface of smooth Brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.994-1002.1978
更新日期:1978-09-01 00:00:00
abstract::The leukotrienes and tumor necrosis factor (TNF) play an important role in the pathophysiology of septic shock, in which hypotension, leukopenia, thrombocytopenia, and hemoconcentration are observed. This study was performed to examine the effects of a 5-lipoxygenase inhibitor (AA-861), a selective leukotriene recepto...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.6.2432-2437.1992
更新日期:1992-06-01 00:00:00
abstract::In the enteric pathogen Vibrio cholerae, expression of the major virulence factors is controlled by the hierarchical expression of several regulatory proteins comprising the ToxR regulon. In this study, we demonstrate that disruption of the fadD gene encoding a long-chain fatty acyl coenzyme A ligase has marked effect...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00663-10
更新日期:2011-01-01 00:00:00
abstract::Our previous studies identified two iron-regulated cytoplasmic membrane proteins of 32 and 36 kDa expressed by both Staphylococcus epidermidis and Staphylococcus aureus. In this study we show by Triton X-114 phase partitioning and tritiated palmitic acid labelling that these proteins are lipoproteins which are anchore...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3767-3774.1998
更新日期:1998-08-01 00:00:00
abstract::Staphylococcal enterotoxins A through D (SEA through SED) and toxic shock syndrome toxin-1 display superantigen properties, i.e., they stimulate a great fraction of T cells expressing certain T-cell receptor V beta sequences. Using a newly established rat model of septic Staphylococcus aureus arthritis, we have recent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.4185-4187.1995
更新日期:1995-10-01 00:00:00
abstract::Various pathogenic bacteria with the capacity to live within eukaryotic cells activate an apoptotic program in infected host cells. Induction of apoptosis by Listeria monocytogenes in murine dendritic cells and hepatocytes has been described. Here we address the questions of whether and how the pathogen kills macropha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4075-4081.1997
更新日期:1997-10-01 00:00:00
abstract::Chronic pulmonary infection with Pseudomonas aeruginosa is a feature of cystic fibrosis (CF) and other chronic lung diseases. Cytokines of the interleukin-17 (IL-17) family have been proposed as important in the host response to P. aeruginosa infection through their role in augmenting antibacterial immune responses, a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00717-16
更新日期:2016-11-18 00:00:00
abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2262-2272.2005
更新日期:2005-04-01 00:00:00
abstract::The level of type-specific antigen (that covalently associated with the cell wall peptidoglycan and that released extracellularly) synthesized by virulent and avirulent strains of type III group B streptococci was quantitated and compared. Additionally, the effect of the physiological age of the cells and the influenc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.217-221.1984
更新日期:1984-05-01 00:00:00
abstract::Using a PCR-based strategy and degenerate oligonucleotides, we isolated a Legionella pneumophila gene that showed high sequence similarity to members of the fliI gene family. An insertion mutation that disrupted the fliI open reading frame was recombined onto the L. pneumophila chromosome and analyzed for its effects ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.6.2497-2501.1997
更新日期:1997-06-01 00:00:00
abstract::Individual strains of Staphylococcus aureus have different capacities to become internalized by osteoblasts. Here we report that the levels of sigma(B) expressed by S. aureus correlate with the capacity of this bacterium to be internalized by osteoblasts. However, sigma(B) is not essential for internalization and does...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.4167-4170.2003
更新日期:2003-07-01 00:00:00
abstract::Pseudomonas aeruginosa utilizes a number of distinct pathways to secrete proteins that play various roles during infection. These include the type II secretion system, which is responsible for the secretion of the majority of exoproducts into the surrounding environment, including toxins and degradative enzymes. In co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1695-1705.2005
更新日期:2005-03-01 00:00:00
abstract::Peptidoglycans of organisms belonging to the strictly anaerobic family Bacteroidaceae were investigated for the presence of lanthionine. Different procedures for the quantitation of lanthionine were compared. Performic acid and peroxide oxidation procedures on 35S-labeled peptidoglycan from Fusobacterium nucleatum Fev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.114-122.1982
更新日期:1982-04-01 00:00:00
abstract::Trypanosoma cruzi is a protozoan parasite that can initiate mucosal infection after conjunctival exposure. The anatomical route of T. cruzi invasion and spread after conjunctival parasite contamination remains poorly characterized. In the present work we have identified the sites of initial invasion and replication af...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00319-06
更新日期:2006-10-01 00:00:00
abstract::An inactivated oral enterotoxigenic Escherichia coli (ETEC) vaccine against ETEC diarrhea was given to 25 adult Swedish volunteers. The vaccine consisted of formalin-killed E. coli bacteria expressing the most common colonization factor antigens (CFAs), i.e., CFA/I, -II, and -IV, and recombinantly produced cholera B s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3311-3316.1998
更新日期:1998-07-01 00:00:00
abstract::We investigated the mechanisms that lead to the production of proinflammatory mediators by human monocytes when these cells are exposed in vitro to live Borrelia burgdorferi spirochetes. We first focused on myeloid differentiation primary response protein 88 (MyD88), an adapter molecule that is essential in the Toll-l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01078-08
更新日期:2009-03-01 00:00:00
abstract::Cytomegalovirus (CMV) infections are common in renal transplant recipients. We studied 23 recipients prospectively to determine whether infections by other herpes-group and non-herpes-group viruses were also present. Sera, obtained at the time of surgery and periodically thereafter, were tested for antibody to CMV, he...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.4.970-975.1976
更新日期:1976-10-01 00:00:00
abstract::In previous work (Jones, Infect, Immun. 30:78-89, 1980) a major cytoplasmic antigen of Candida albicans was identified. In both humans and experimental animals, this antigen is released from C. albicans during the course of an invasive C. albicans infection and elicits a specific antibody response. In this study, we u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.469-477.1981
更新日期:1981-11-01 00:00:00
abstract::Efficient utilization of heme as an iron (Fe) source by Bordetella avium requires bhuR, an Fe-regulated gene which encodes an outer membrane heme receptor. Upstream of bhuR is a 507-bp open reading frame, hereby designated rhuI (for regulator of heme uptake), which codes for a 19-kDa polypeptide. Whereas the 19-kDa po...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.6951-6961.2001
更新日期:2001-11-01 00:00:00
abstract::In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (CPEK). Invasion was examined by the gentamicin protection ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00542-15
更新日期:2015-10-01 00:00:00
abstract::The question of whether Campylobacter jejuni produces a cholera toxin-like toxin (CTLT) has been controversial. The objective of this study was to identify the factor that cross-reacts with CT from C. jejuni. Filtrates of C. jejuni grown in four different liquid media reported to promote CTLT production were tested by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00139-07
更新日期:2007-06-01 00:00:00
abstract::A murine immunoglobulin G monoclonal antibody (MAb) raised against outer membrane vesicles of Moraxella catarrhalis 035E was shown to bind to a surface-exposed epitope of a major outer membrane protein of this organism. This outer membrane protein, which had an apparent molecular weight of approximately 80,000 in sodi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.2003-2010.1993
更新日期:1993-05-01 00:00:00
abstract::Henle 407 and HeLa cells were compared as hosts for Shigella dysenteriae at a low multiplicity of infection. Efficiency of attachment and invasion without centrifugation, as well as selectivity for pathogenic over nonpathogenic S. dysenteriae without Congo red, were much greater for Henle 407 cells than for HeLa cells...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.7.2401-2403.1990
更新日期:1990-07-01 00:00:00
abstract::Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.2.945-952.2002
更新日期:2002-02-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi) is a commensal microorganism of the human nasopharynx, and yet is also an opportunistic pathogen of the upper and lower respiratory tracts. Host microenvironments influence gene expression patterns, likely critical for NTHi persistence. The host sequesters iron as a mechanism ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01227-12
更新日期:2013-04-01 00:00:00
abstract::Administration of a standard bacterial vaccine (SBV) affords some protection against influenza infection in mice if given 4 to 5 h before inoculation of the virus. This effect was enhanced by repeated injections of SBV 7 and 14 days earlier, as measured by the length of survival, mortality rate, development of gross p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.5.771-776.1973
更新日期:1973-05-01 00:00:00
abstract::Binding of the complement-downregulating protein factor H (fH) to the surface of the meningococcus is important for survival of the organism in human serum. The meningococcal vaccine candidate factor H binding protein (fHbp) is an important ligand for human fH. While some fHbp-specific monoclonal antibodies (MAbs) blo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05182-11
更新日期:2011-09-01 00:00:00
abstract::Cell wall lytic enzyme (Kyowa lytic no. 2 enzyme) liberated arthritogenic hydrosoluble peptidoglycans from both arthritogenic and non-arthritogenic bacterial cell walls. From these cell walls, mutanolysin (peptidoglycan-degrading enzyme) also liberated hydrosoluble peptidoglycans which, however, lacked arthritogenicit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.861-866.1977
更新日期:1977-06-01 00:00:00
abstract::A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.439-444.1977
更新日期:1977-05-01 00:00:00