Two proto-oncogenes implicated in mammary carcinogenesis, int-1 and int-2, are independently regulated during mouse development.

abstract：:We induced sublimation of suspended few-layer graphene by in situ Joule-heating inside a transmission electron microscope. The graphene sublimation fronts consisted of mostly {1100} zigzag edges. Under appropriate conditions, a fractal-like "coastline" morphology was observed. Extensive multiple-layer reconstructions ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang JY,Ding F,Yakobson BI,Lu P,Qi L,Li J

更新日期：2009-06-23 00:00:00

abstract：:Frontotemporal dementia with parkinsonism, chromosome 17 type (FTDP-17) is caused by mutations in the tau gene, and the signature lesions of FTDP-17 are filamentous tau inclusions. Tau mutations may be pathogenic either by altering protein function or gene regulation. Here we show that missense, silent, and intronic t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： D'Souza I,Poorkaj P,Hong M,Nochlin D,Lee VM,Bird TD,Schellenberg GD

更新日期：1999-05-11 00:00:00

abstract：:Thiostrepton (TSR), an archetypal bimacrocyclic thiopeptide antibiotic that arises from complex posttranslational modifications of a genetically encoded precursor peptide, possesses a quinaldic acid (QA) moiety within the side-ring system of a thiopeptide-characteristic framework. Focusing on selective engineering of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng Q,Wang S,Duan P,Liao R,Chen D,Liu W

更新日期：2016-12-13 00:00:00

abstract：:Profilin1, a ubiquitously expressed actin-binding protein, plays a critical role in cell migration through actin cytoskeletal regulation. Given the traditional view of profilin1 as a promigratory molecule, it is difficult to reconcile observations that profilin1 is down-regulated in various invasive adenocarcinomas an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bae YH,Ding Z,Das T,Wells A,Gertler F,Roy P

更新日期：2010-12-14 00:00:00

abstract：:The detailed molecular dynamics of an actual bond-breaking event in a fluctuating enzyme substrate complex is simulated. The method developed allows one to explore what type of fluctuations are involved in enzymatic reactions and to evaluate entropic contributions to enzyme catalysis. The fluctuations of the enzyme el...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warshel A

更新日期：1984-01-01 00:00:00

abstract：:The VX2 carcinoma-bearing rabbit is an animal model for tumor-induced hypercalcemia, thought to be due to increased bone destruction effected by prostaglandin E2. The present experiments suggest that the pathophysiology of the hypercalcemia differs from that previously proposed. Tumor was transplanted intramuscularly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doppelt SH,Slovik DM,Neer RM,Nolan J,Zusman RM,Potts JT Jr

更新日期：1982-01-01 00:00:00

abstract：:Weber's law can be explained either by a compressive scaling of sensory response with stimulus magnitude or by a proportional scaling of response variability. These two mechanisms can be distinguished by asking how quantities are added or subtracted. We trained Rhesus monkeys to associate 26 distinct symbols with 0-25...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Livingstone MS,Pettine WW,Srihasam K,Moore B,Morocz IA,Lee D

更新日期：2014-05-06 00:00:00

abstract：:The sloppy paired (slp) locus consists of two genes, slp1 and slp2, both of which encode proteins containing a forkhead domain (a DNA-binding motif). Previous work has shown that a severe segmentation phenotype is obtained only when both slp genes are deleted. Here we examine the functional redundancy of the locus in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cadigan KM,Grossniklaus U,Gehring WJ

更新日期：1994-07-05 00:00:00

abstract：:Sympathetic activation of β-adrenoreceptors (β-AR) represents a hallmark in the development of heart failure (HF). However, little is known about the underlying mechanisms of gene regulation. In human ventricular myocardium from patients with end-stage HF, we found high levels of phosphorylated histone 3 at serine-28 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saadatmand AR,Sramek V,Weber S,Finke D,Dewenter M,Sticht C,Gretz N,Wüstemann T,Hagenmueller M,Kuenzel SR,Meyer-Roxlau S,Kramer M,Sossalla S,Lehmann LH,Kämmerer S,Backs J,El-Armouche A

更新日期：2019-10-29 00:00:00

abstract：:The rat W3/25 antigen was the first marker antigen of helper T lymphocytes to be identified. Subsequently, the human OKT4 antigen (now called CD4) was described, and cell distribution and functional data suggested that W3/25 and OKT4 antigens were homologous. This is now confirmed by the matching of peptide sequences ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark SJ,Jefferies WA,Barclay AN,Gagnon J,Williams AF

更新日期：1987-03-01 00:00:00

abstract：:The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF and might play an important role in neoplastic development. Using RNA blot hybridization, we have found that exposure o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark AJ,Ishii S,Richert N,Merlino GT,Pastan I

更新日期：1985-12-01 00:00:00

abstract：:Exposure of cholera toxin to membrane particles prepared from sarcoma 180 cells gives rise to a variety of fragments which are capable of activating adenylate cyclase [ATP:pyrophosphate-lyase (cyclizing), EC 4.6.1.1]. A major component of these fragments has an apparent molecular weight in the 8,000-10,000 range. The ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matuo Y,Wheeler MA,Bitensky MW

更新日期：1976-08-01 00:00:00

abstract：:A central question in protein-DNA recognition is the origin of the specificity that permits binding to the correct site in the presence of excess, nonspecific DNA. In the P22 Arc repressor, the Phe-10 side chain is part of the hydrophobic core of the free protein but rotates out to pack against the sugar-phosphate bac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schildbach JF,Karzai AW,Raumann BE,Sauer RT

更新日期：1999-02-02 00:00:00

abstract：:In vivo electroporation is a powerful technique for the introduction of genes into organisms. Temporal and spatial regulation of expression of introduced genes, or of RNAi, would further enhance the utility of this method. Here we demonstrate conditional regulation of gene expression from electroporated plasmids in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsuda T,Cepko CL

更新日期：2007-01-16 00:00:00

abstract：:We describe the use of the matrix eigenvalue decomposition (EVD) and pseudoinverse projection and a tensor higher-order EVD (HOEVD) in reconstructing the pathways that compose a cellular system from genome-scale nondirectional networks of correlations among the genes of the system. The EVD formulates a genes x genes n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alter O,Golub GH

更新日期：2005-12-06 00:00:00

abstract：:The lateral mobility of individual murine polyoma virus-like particles (VLPs) bound to live cells and artificial lipid bilayers was studied by single fluorescent particle tracking using total internal reflection fluorescence microscopy. The particle trajectories were analyzed in terms of diffusion rates and modes of m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ewers H,Smith AE,Sbalzarini IF,Lilie H,Koumoutsakos P,Helenius A

更新日期：2005-10-18 00:00:00

abstract：:Poly(A)+ mRNA was isolated from the brains of C57BL/6J (B6), DBA/2J (D2), and F1 hybrid mice (B6 X D2) of 16-17 days of age. The yield of polysomal RNA, both poly(A)+ and poly(A)-, from the three strains of mice was comparable. When translated in vitro in a reticulocyte lysate system, the mRNA preparations had the sam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miskimins R,Ebato H,Seyfried TN,Yu RK

更新日期：1986-03-01 00:00:00

abstract：:We have established empirical limits for the circular dichroism spectra appropriate for aqueous solutions of the B, C, and A forms of calf-thymus DNA and have analyzed DNA conformations in biological structures. The circular dichroism spectrum above 250 nm of purified calf-thymus chromatin can be satisfactorily accoun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hanlon S,Johnson RS,Wolf B,Chan A

更新日期：1972-11-01 00:00:00

abstract：:Whether Golgi enzymes remain localized within the Golgi or constitutively cycle through the endoplasmic reticulum (ER) is unclear, yet is important for understanding Golgi dependence on the ER. Here, we demonstrate that the previously reported inefficient ER trapping of Golgi enzymes in a rapamycin-based assay results...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sengupta P,Satpute-Krishnan P,Seo AY,Burnette DT,Patterson GH,Lippincott-Schwartz J

更新日期：2015-12-08 00:00:00

abstract：:The specific ornithine decarboxylase inhibitor alpha-difluoromethylornithine, when given to adult rats in vivo for 5 wk, resulted in a decrease in peripheral blood cell elements in normal rats and a marked suppression of marrow recovery in rats with chemotherapy-induced marrow hypoplasia. In normal rats, alpha-difluor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luk GD,Sharkis SJ,Abeloff MD,McCann PP,Sjoerdsma A,Baylin SB

更新日期：1983-08-01 00:00:00

abstract：:Vertebrates can modulate the sound levels entering their inner ears in the face of intense external sound or during their own vocalizations. Middle ear muscle contractions restrain the motion of the middle ear ossicles, attenuating the transmission of low-frequency sound and thereby protecting the hair cells in the in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gridi-Papp M,Feng AS,Shen JX,Yu ZL,Rosowski JJ,Narins PM

更新日期：2008-08-05 00:00:00

abstract：:Keratins 14 and 5 are the structural hallmarks of the basal keratinocytes of the epidermis and outer root sheath (ORS) of the hair follicle. Their genes are controlled in a tissue-specific manner and thus serve as useful tools to elucidate the regulatory mechanisms involved in keratinocyte-specific transcription. Prev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sinha S,Fuchs E

更新日期：2001-02-27 00:00:00

abstract：:Three lines of evidence for abrupt tropical climate change, both past and present, are presented. First, annually and decadally averaged delta(18)O and net mass-balance histories for the last 400 and 2,000 yr, respectively, demonstrate that the current warming at high elevations in the mid- to low latitudes is unprece...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson LG,Mosley-Thompson E,Brecher H,Davis M,León B,Les D,Lin PN,Mashiotta T,Mountain K

更新日期：2006-07-11 00:00:00

abstract：:Columnar stromatolites were abundant and widespread in the Proterozoic but are exceedingly rare in modern seas. Consequently, the stromatolites at Hamelin Pool in Shark Bay, Western Australia, have been widely interpreted as unique modern analogs of ancient stromatolites constructed by complex communities of cyanobact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Awramik SM,Riding R

更新日期：1988-03-01 00:00:00

abstract：:Biotin plays an essential role in growth of mycobacteria. Synthesis of the cofactor is essential for Mycobacterium tuberculosis to establish and maintain chronic infections in a murine model of tuberculosis. Although the late steps of mycobacterial biotin synthesis, assembly of the heterocyclic rings, are thought to f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu Z,Cronan JE

更新日期：2020-09-22 00:00:00

abstract：:The four-helical bundle soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor (SNARE) complex that mediates intracellular membrane fusion events contains a highly conserved ionic layer at the center of an otherwise hydrophobic core. This layer has an undetermined function; it consists of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scales SJ,Yoo BY,Scheller RH

更新日期：2001-12-04 00:00:00

abstract：:Eukaryotic DNA replication fidelity relies on the concerted action of DNA polymerase nucleotide selectivity, proofreading activity, and DNA mismatch repair (MMR). Nucleotide selectivity and proofreading are affected by the balance and concentration of deoxyribonucleotide (dNTP) pools, which are strictly regulated by r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt TT,Reyes G,Gries K,Ceylan CÜ,Sharma S,Meurer M,Knop M,Chabes A,Hombauer H

更新日期：2017-05-30 00:00:00

abstract：:Coincidence cloning allows the isolation of sequences held in common by two genomic DNA populations. Human DNA from two human-hamster hybrid cell lines was amplified by Alu-repeat primers (Alu PCR) and the products originating from the shared human chromosomal region were cloned. To achieve this, human sequences were ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aslanidis C,de Jong PJ

更新日期：1991-08-01 00:00:00

abstract：:Cultured fibroblast cells derived from a skin biopsy sample taken from normal human adult were exposed to a potent carcinogen, 4-nitroquinoline 1-oxide. Alterations of cell growth pattern such as higher density and piling up of cells were noticed in some fractions of cultures that were successively subcultured after n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kakunaga T

更新日期：1978-03-01 00:00:00

abstract：:Two monoclonal antibodies directed against the native beta 2 subunit of Escherichia coli tryptophan synthase [L-serine hydro-lyase (adding indoleglycerol-phosphate), EC 4.2.1.20], one recognizing the C-terminal F1 domain and the other the N-terminal F2 domain, were used to detect immunoreactive intermediates in the fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blond S,Goldberg M

更新日期：1987-03-01 00:00:00