Abstract：
:Spatial organization of G-protein coupled receptors (GPCRs) into dimers and higher order oligomers has been demonstrated in vitro and in vivo. The pharmacological readout was shown to depend on the specific interfaces, but why particular regions of the GPCR structure are involved, and how ligand-determined states change them remains unknown. Here we show why protein-membrane hydrophobic matching is attained upon oligomerization at specific interfaces from an analysis of coarse-grained molecular dynamics simulations of the spontaneous diffusion-interaction of the prototypical beta2-adrenergic (β2AR) receptors in a POPC lipid bilayer. The energy penalty from mismatch is significantly reduced in the spontaneously emerging oligomeric arrays, making the spatial organization of the GPCRs dependent on the pattern of mismatch in the monomer. This mismatch pattern is very different for β2AR compared to the highly homologous and structurally similar β1AR, consonant with experimentally observed oligomerization patterns of β2AR and β1AR. The results provide a mechanistic understanding of the structural context of oligomerization.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Mondal S,Johnston JM,Wang H,Khelashvili G,Filizola M,Weinstein Hdoi
10.1038/srep02909subject
Has Abstractpub_date
2013-10-09 00:00:00pages
2909issn
2045-2322pii
srep02909journal_volume
3pub_type
杂志文章abstract：:Keloid is the abnormal wound healing puzzled by the aggressive growth and high recurrence rate due to its unrevealed key pathogenic mechanism. MicroRNAs contribute to a series of biological processes including epithelial-mesenchymal transition (EMT) and cells stemness involved in fibrotic disease. Here, using microRNA...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28281
更新日期：2016-09-06 00:00:00
abstract：:Studies have reported that neck and/or shoulder pain (NSP) was prevalent and a non-ignorable occupational health problem in healthcare providers. Considering data deficiency on NSP, we aimed to investigate the prevalence and sick leave absence of NSP among public hospital workers in Shandong, China and to explore the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69382-4
更新日期：2020-07-23 00:00:00
abstract：:A key to the success of chimeric antigen receptor (CAR) T-cell based therapies greatly rely on the capacity to identify and target antigens with expression restrained to tumor cells. Here we present a strategy to generate CAR T-cells that are only effective locally (tumor tissue), potentially also increasing the choic...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39833
更新日期：2017-01-20 00:00:00
abstract：:Printing techniques are becoming increasingly prevalent in modern manufacturing. However, its biggest drawback is the limit in printing resolution. In this paper, we present solvodynamic printing as a novel printing system which aims to improve print resolution by incorporating an additional immiscible carrier solvent...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47105-8
更新日期：2019-07-24 00:00:00
abstract：:Enhancement of endogenous neurogenesis after ischemic stroke may improve functional recovery. We previously demonstrated that medium B, which is a combination with epidermal growth factor (EGF) and fibronectin, can promote neural stem/progenitor cell (NSPC) proliferation and migration. Here, we showed that medium B pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63598-0
更新日期：2020-04-20 00:00:00
abstract：:Chronic inflammatory insults compromise immune cell responses and ultimately contribute to pathologic outcomes. Clinically, it has been suggested that bone debris and implant particles, such as polymethylmethacrylate (PMMA), which are persistently released following implant surgery evoke heightened immune, inflammator...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46934-x
更新日期：2019-07-18 00:00:00
abstract：:Modern Computed Tomography (CT) scanners are capable of acquiring contrast dynamics of the whole brain, adding functional to anatomical information. Soft tissue segmentation is important for subsequent applications such as tissue dependent perfusion analysis and automated detection and quantification of cerebral patho...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00239-z
更新日期：2017-03-09 00:00:00
abstract：:Glycyrrhetinic acid monoglucuronide (GAMG) is a great value-added and has considerable commercial interest due to its strong pharmacological activities and functional low-calorie sweetener. However GAMG is quite rare in natural plants, and it must be prepared from glycyrrhizin (GL) by hydrolysing one terminal glucuron...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10154-y
更新日期：2017-10-02 00:00:00
abstract：:Barley is a kind of cereal grass belonging to the family Poaceae. To examine viruses infecting winter barley in Korea, we carried out a comprehensive study of barley RNA viromes using next-generation sequencing (NGS). A total of 110 barley leaf samples from 17 geographical locations were collected. NGS followed by ext...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31671-4
更新日期：2018-09-05 00:00:00
abstract：:Pyramidal neurons in the rodent hippocampus exhibit spatial tuning during spatial navigation, and they are reactivated in specific temporal order during sharp-wave ripples observed in quiet wakefulness or slow wave sleep. However, analyzing representations of sleep-associated hippocampal ensemble spike activity remain...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32193
更新日期：2016-08-30 00:00:00
abstract：:Classification of histologic patterns in lung adenocarcinoma is critical for determining tumor grade and treatment for patients. However, this task is often challenging due to the heterogeneous nature of lung adenocarcinoma and the subjective criteria for evaluation. In this study, we propose a deep learning model tha...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40041-7
更新日期：2019-03-04 00:00:00
abstract：:Mitochondria play critical roles in meeting cellular energy demand, in cell death, and in reactive oxygen species (ROS) and stress signaling. Most Caenorhabditis elegans loss-of-function (lf) mutants in nuclear-encoded components of the respiratory chain are non-viable, emphasizing the importance of respiratory functi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29695
更新日期：2016-07-21 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-44437-3
更新日期：2019-06-05 00:00:00
abstract：:Many efforts have been made in the last 30 years to develop more relevant in vitro models to study genotoxic responses of drugs and environmental contaminants. While 2D HepaRG cells are one of the most promising models for liver toxicology, a switch to 3D cultures that integrate both in vivo architecture and cell-cell...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47114-7
更新日期：2019-07-22 00:00:00
abstract：:With the reduction of oil and gas reserves and the increase of mining difficulty in Northern China, the carbonate rocks in Southern North China Basin are becoming a significant exploration target for carbonate reservoirs. However, the development characteristics, formation stages, formation environments and mechanisms...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80878-x
更新日期：2021-01-12 00:00:00
abstract：:Human NAD-dependent isocitrate dehydrogenase catalyzes the decarboxylation of isocitrate (ICT) into α-ketoglutarate in the Krebs cycle. It exists as the α2βγ heterotetramer composed of the αβ and αγ heterodimers. Previously, we have demonstrated biochemically that the α2βγ heterotetramer and αγ heterodimer can be allo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40921
更新日期：2017-01-18 00:00:00
abstract：:The human ether-à-go-go related gene (hERG) channel is crucial for the cardiac action potential by contributing to the fast delayed-rectifier potassium current. Mutations in the hERG channel result in type 2 long QT syndrome (LQT2). The hERG channel contains a cyclic nucleotide-binding homology domain (CNBHD) and this...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23712
更新日期：2016-03-30 00:00:00
abstract：:Survivin is an independent prognostic factor for joint destruction in rheumatoid arthritis (RA). However, the expression and function of survivin in RA synoviocytes remain unclear. We certified the expression of survivin in RA synovial tissues and performed the experiment using RA fibroblast-like synoviocytes (RA-FLS)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09795
更新日期：2015-05-22 00:00:00
abstract：:Lower cervical dislocation with facet interlocking is one of the most drastic injuries to the cervical spine. The early reduction is thought critical in preventing progressive secondary spinal cord injury. The authors report a new surgical procedure in the management of lower cervical dislocation with facet interlocki...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37742-w
更新日期：2019-02-04 00:00:00
abstract：:Although intestinal trefoil factor (ITF) can alleviate the burn-induced intestinal mucosa injury, the underlying mechanisms remains elusive. In this study, we investigated if ITF alters glutamine transport on the brush border membrane vesicles (BBMVs) of the intestines in Sprague-Dawley rats inflicted with 30% TBSA an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21282-4
更新日期：2018-02-16 00:00:00
abstract：:Angiotensin II (AngII) promotes hypertension, atherogenesis, vascular aneurysm and impairs post-ischemic cardiac remodeling through concerted roles on vascular cells, monocytes and T lymphocytes. However, the role of AngII in B lymphocyte responses is largely unexplored. Here, we show that chronic B cell depletion (Ba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04438-6
更新日期：2017-06-23 00:00:00
abstract：:Large-area, pinhole-free CH3NH3PbI3 perovskite thin films were successfully fabricated on 5 cm × 5 cm flexible indium tin oxide coated polyethylene naphthalate (ITO-PEN) substrates through a sequential evaporation/spin-coating deposition method in this research. The influence of the rate-controlled evaporation of PbI2...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18970-y
更新日期：2018-01-11 00:00:00
abstract：:There are more than 2300 genes that are predominantly expressed in mouse testes. The role of hundreds of these genes has been studied in mouse spermatogenesis but still there are many genes whose function is unknown. Gene knockout (KO) strategy in mice is widely used for in vivo study of gene function. The present stu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23176-x
更新日期：2018-03-21 00:00:00
abstract：:The stress distribution around endosseous implants is an important determinant of the surgical success. However, no method developed so far to determine the implant stability is sensitive to the loading conditions of the bone-implant interface (BII). The objective of this study is to investigate whether a quantitative...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50665-4
更新日期：2019-10-02 00:00:00
abstract：:The central challenge in realizing non-volatile, E-field manipulation of magnetism lies in finding an energy efficient means to switch between the distinct magnetic states in a stable and reversible manner. In this work, we demonstrate using electrical polarization-induced charge screening to change the ground state o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07740
更新日期：2015-01-13 00:00:00
abstract：:The sensitivity and accuracy of the Risk/Injury/Failure/Loss/End-stage (RIFLE) versus acute kidney injury Network (AKIN) criteria for acute kidney injury (AKI) in critically ill patients remains uncertain. Therefore, we performed a systematic review and meta-analysis to investigate the incidence and prognostic value o...
journal_title：Scientific reports
pub_type： 杂志文章,meta分析
doi：10.1038/srep17917
更新日期：2015-12-07 00:00:00
abstract：:The Przewalski's gazelle (Procapra przewalskii) is one of the most endangered ungulates in the world, with fewer than 2,000 individuals surviving in nine habitat fragments on the Qinghai-Tibet Plateau and isolated by human settlements and infrastructure. In particular, the Qinghai-Tibet railway, which crosses the larg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18163-7
更新日期：2017-12-21 00:00:00
abstract：:Antimicrobial stewardship programmes (ASP) are aimed at optimising antimicrobial utilization. However, only few studies have focused on paediatric intensive care units (PICU), where inappropriate antibiotic use occurs frequently. We assessed the effect and safety of a once weekly paediatric infectious disease (PID) wa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65671-0
更新日期：2020-06-01 00:00:00
abstract：:A host of hormonal-metabolic alterations take place following exposure of cardiomyocytes to hypoxia and other noxious stimuli. Here, we demonstrate that exposure of cultured rat cardiomyocytes to lipopolysaccharide (LPS) resulted in upregulation (~1.5 fold) of oxidized low-density lipoprotein receptor-1 (LOX-1). There...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55694-7
更新日期：2019-12-17 00:00:00
abstract：:Rapid facial mimicry (RFM) is an automatic response, in which individuals mimic others' expressions. RFM, only demonstrated in humans and apes, is grounded in the automatic perception-action coupling of sensorimotor information occurring in the mirror neuron system. In humans, RFM seems to reflect the capacity of indi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01527
更新日期：2013-01-01 00:00:00
