An oxygen sensitive self-decision making engineered CAR T-cell.

Abstract:

:A key to the success of chimeric antigen receptor (CAR) T-cell based therapies greatly rely on the capacity to identify and target antigens with expression restrained to tumor cells. Here we present a strategy to generate CAR T-cells that are only effective locally (tumor tissue), potentially also increasing the choice of targetable antigens. By fusing an oxygen sensitive subdomain of HIF1α to a CAR scaffold, we generated CAR T-cells that are responsive to a hypoxic environment, a hallmark of certain tumors. Along with the development of oxygen-sensitive CAR T-cells, this work also provides a basic framework to use a multi-chain CAR as a platform to create the next generation of smarter self-decision making CAR T-cells.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Juillerat A,Marechal A,Filhol JM,Valogne Y,Valton J,Duclert A,Duchateau P,Poirot L

doi

10.1038/srep39833

subject

Has Abstract

pub_date

2017-01-20 00:00:00

pages

39833

issn

2045-2322

pii

srep39833

journal_volume

7

pub_type

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