Abstract：
OBJECTIVE:To identify and characterize a novel sperm motility-stimulating protein (MSP) from caprine serum. DESIGN:Prospective experimental study. SETTING:Research laboratory. ANIMAL(S):Rabbits and male and female BALB/c mice. INTERVENTION(S):Protein purification by conventional methods and functional and immunological characterization. MAIN OUTCOME MEASURE(S):MSP was purified, purity was checked, and molecular weight was determined. Surface localization, tissue distribution, and IVF for contraceptive efficacy were studied. RESULT(S):MSP is a heat-stable 66-kDa monomeric novel protein. At 0.9 μM, it showed much higher forward motility and longer motility maintenance than other known activators. Studies with the motility analyzers CASA and SPERMA showed an increase in horizontal and vertical velocities, respectively. MSP action was cyclic AMP independent. Its occurrence was higher in testis, although blood was the richest source. MSP was localized throughout the cell surface of spermatozoa. Its antibody caused significant inhibition of sperm motility and inhibited fertilization up to 100% at 1:25 dilution, which showed its contraceptive efficacy. CONCLUSION(S):MSP has high efficacy to stimulate sperm forward motility and thus may be used in biomedical application in infertility clinics, animal breeding centers, poultries, and animal conservation centers.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Saha S,Das S,Bhoumik A,Ghosh P,Majumder GC,Dungdung SRdoi
10.1016/j.fertnstert.2013.03.012subject
Has Abstractpub_date
2013-07-01 00:00:00pages
269-79.e1-5issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(13)00413-5journal_volume
100pub_type
杂志文章abstract：OBJECTIVE:To investigate endometrial dating during the conception cycle. DESIGN:Endometrial biopsies of the last half of the luteal phase of conception cycles were dated based on urinary luteinizing hormone (LH) surges. SETTING:Endometrial samples were obtained from women attending the fertility clinic of the Departm...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：OBJECTIVE:To present a case report of a spontaneous, heterotopic, triplet conception of a patient without any risk factors. DESIGN:Case report. SETTING:Community hospital in Park Ridge, Illinois. PATIENT(S):One patient, 30-year-old multigravida, sent to the emergency room for evaluation of right lower quadrant pain ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.12.034
更新日期：2007-10-01 00:00:00
abstract：OBJECTIVE:To investigate the optimal protocol for cryopreservation of human embryos obtained from IVF. DESIGN:Prospective randomized study. SETTING:Consenting patients in an academic research environment. PATIENTS:Couples undergoing IVF. INTERVENTIONS:A cohort of 2,220 supernumerary multicellular embryos were obtai...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1995-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effects of a prolonged duration of gonadotropin-releasing hormone agonist (GnRH-a) in pituitary down-regulation for controlled ovarian hyperstimulation (COH) on the live-birth rate in nonendometriotic women undergoing in vitro fertilization and embryo transfer (IVF-ET). DESIGN:Retrospective c...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.03.030
更新日期：2014-07-01 00:00:00
abstract：:Tumor necrosis factor-alpha stimulates the secretion of vascular endothelial growth factor by cultured human oviductal epithelial cells and stromal fibroblasts. Tumor necrosis factor-alpha-stimulated vascular endothelial growth factor secretion by these cells may control oviductal fluid secretion by regulating vascula...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.05.082
更新日期：2007-01-01 00:00:00
abstract：OBJECTIVE:To study social and demographic differentiation of assisted reproduction technology (ART) use at the population level in the United States. DESIGN:Population-based study. SETTING:Not applicable. PATIENT(S):Women 15-49 years old in the American Community Survey and National Vital Statistics Birth Certificat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2019.07.1323
更新日期：2019-12-01 00:00:00
abstract：OBJECTIVE:To analyze the potential role of extraovarian factors such as endometrium in the reproductive outcome of obese patients. DESIGN:Retrospective study. SETTING:University-affiliated infertility clinic, between January 2001 and July 2005. PATIENT(S):Women undergoing a total of 2656 first ovum donation (OD) cyc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.11.162
更新日期：2007-08-01 00:00:00
abstract：:Ovarian hyperstimulation syndrome (OHSS) is one of the most serious, and potentially lethal, complications of controlled ovarian stimulation (COS). Induction of final oocyte maturation with a bolus of gonadotropin-releasing hormone (GnRH) agonist (GnRHa), instead of the criterion standard hCG, in patients undergoing o...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2015.02.004
更新日期：2015-04-01 00:00:00
abstract：:123 healthy Jewish women (18-22 years) were divided into OC (oral contraceptives) users (n=75), and controls (n=48). All women were on a similar diet. The OC users were divided into 3 groups (1, 2 and 3) based on the OC preparations they were taking. Venous blood samples were collected; serum copper levels were mea...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44366-9
更新日期：1979-11-01 00:00:00
abstract：OBJECTIVE:To explore the value of serum vascular endothelial growth factor (VEGF) concentrations during IVF cycles in predicting the risk of ovarian hyperstimulation syndrome (OHSS). DESIGN:Prospective study. SETTING:London Women's Clinic. PATIENT(S):One hundred seven women undergoing IVF. Mild OHSS developed in 10 ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00447-6
更新日期：1999-02-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to determine the positive predictive value (PPV) and negative predictive value (NPV) of FISH analysis and to determine which chromosomal abnormalities are most frequently confirmed. DESIGN:Prospective observational. SETTING:IVF laboratory. PATIENT(S):Two hundred forty-one embr...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.07.1337
更新日期：2008-10-01 00:00:00
abstract：:Hysteroscopic examination of the uterine cavity revealed that patients previously treated for intramural myoma(s) by uterine artery embolization had a significantly higher incidence of abnormal findings compared with patients treated by laparoscopic occlusion of uterine arteries (59.5% vs. 2.7%). In particular, there ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2010.12.014
更新日期：2011-05-01 00:00:00
abstract：:A uteroglobin-like molecule was detected with a sensitive radioimmunoassay for rabbit uteroglobin in the uterine washings of women. This antigen was detectable only in small amounts in the uterine washings obtained from women in the late luteal phase or proliferative phase, but was detected in large amounts in uterine...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)49399-4
更新日期：1986-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the association of an activating single-nucleotide polymorphism (SNP) at position -71 of the promoter of 17beta-hydroxysteroid dehydrogenase type 5 gene (-71A/G HSD17B5 SNP) and polycystic ovary syndrome (PCOS) in a well characterized cohort of caucasian PCOS women with biochemical hyperandrogenem...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.06.016
更新日期：2009-08-01 00:00:00
abstract：:Women who exercise heavily may develop secondary amenorrhea. Since the mechanism of so-called "runner's amenorrhea" has not been conclusively established, the authors examined the occurrence of amenorrhea in one of the most intensively exercising groups of female runners in the United States (average, 70 miles/week): ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:In a study of 29 cycles of IVF, ET was performed on day 5 after oocyte recovery when embryos had developed to the morula/blastocyst stage. Three preclinical pregnancies and three live births resulted (2 singleton and 1 twin), giving a viable PR per ET of 10%. It is concluded that while day 5 ET may well be important i...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54257-5
更新日期：1991-04-01 00:00:00
abstract：OBJECTIVE:To report a case of ovarian pregnancy following in vitro fertilization-embryo transfer (IVF-ET) treatment for which the cornual fistulae was the most probable explanation of the cause. DESIGN:Case report. SETTING:University department and assisted reproduction unit. PATIENT(S):A 29-year-old woman with prim...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.06.054
更新日期：2005-01-01 00:00:00
abstract：:We present a rare case of a congenital isolated missing segment of the fallopian tube, including hysterosalpingographic and laparoscopic images. We conclude that when this occurs without concomitant müllerian anomalies, the mechanism of development would not be expected to be associated with an increase in renal abnor...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.01.012
更新日期：2006-06-01 00:00:00
abstract：:Klemetti's birth registry study is put in context of other major studies. Summary advice is offered to practitioners as to what they should tell parents who conceive with assisted reproductive technology. ...
journal_title：Fertility and sterility
pub_type： 评论,信件
doi：10.1016/j.fertnstert.2005.04.067
更新日期：2005-11-01 00:00:00
abstract：:Thrombospondin-1 messenger RNA and protein levels in cultured human endometrial stromal cells (ESCs) treated with 17-beta estradiol (10 nM) were reduced by 47.6% (+/-6.5% SD; P < 0.05) and 49.0% (+/-8.6%; P < 0.05) compared with untreated cells, whereas thrombospondin-1mRNA and protein levels in ESCs treated with prog...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.06.060
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVES:To determine, in a rabbit model, whether peritoneal exposure to dermoid cyst material produces inflammation and adhesions above control levels and whether saline lavage reduces the degree of peritoneal reaction. DESIGN:A prospective, randomized, blinded, controlled study of adhesion formation. Thirty New Ze...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)58225-9
更新日期：1996-04-01 00:00:00
abstract：OBJECTIVE:To develop a consensus regarding the need for luteal phase support during assisted reproductive technology (ART), and to establish the optimal compound and route of administration for this purpose. DESIGN:Review of the published literature on luteal phase support. PATIENT(S):Women undergoing assisted reprod...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/s0015-0282(01)02961-2
更新日期：2002-02-01 00:00:00
abstract：OBJECTIVE:To compare the incidence of noncompliance measured objectively by a home use fertility monitor with the traditional self-reported incidence of compliance in a study of a new method of contraception. DESIGN:Prospective cohort study. SETTING:A large family planning clinic in Edinburgh. PATIENT(S):Thirty-two ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02899-0
更新日期：2001-12-01 00:00:00
abstract：OBJECTIVE:To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. DESIGN:Experimental prospective study. SETTING:University research laboratory and private in vitro fertilization (IVF) clinic. PATI...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2010.06.076
更新日期：2011-01-01 00:00:00
abstract：:A group of 216 vasovasostomized men were tested with the mixed antiglobulin reaction for immunoglobulin (Ig)G, IgA, and secretory IgA antisperm antibodies bound to the sperm membrane. Free antisperm antibodies in serum and seminal plasma were detected with the gelatin agglutination test and the tray agglutination test...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Whereas the dose of hMG is significantly correlated with both BMI and BW, commencing the more obese patient on higher hMG doses is not justified in view of the cancellation of cycles from over-responsiveness in the more obese patients started on higher hMG dosage regimens. If the more obese patient is slow to respond,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55276-5
更新日期：1992-09-01 00:00:00
abstract：OBJECTIVE:To describe a unique case of descending salpingitis and functioning endometrium in a noncavitated mullerian remnant in a patient with Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome. DESIGN:Case report. SETTING:Second Department of Obstetrics and Gynecology, University of Athens, Aretaieion Hospital, Athens,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.11.071
更新日期：2005-05-01 00:00:00
abstract：:Because spermatocyte meiotic error results in embryonic sex chromosomal aneuploidy, it is speculated that teratospermia correlates with increased embryonic sex chromosomal abnormalities. Our findings contradict this theory, suggesting that morphology does not correlate with sex chromosomal genotype. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.07.1006
更新日期：2010-02-01 00:00:00
abstract：:The result of the hormone concentration of one blood sample was used to determine the accuracy of predicting not only the hormone concentrations of a second and third sample drawn 15 minutes apart, but also the mean value of the three samples. Three blood specimens from 73 men involved in two previously reported studi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59662-9
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:To review the literature concerning the regulation of follicle growth, to describe a model for unifollicular ovulation based upon the information gleaned, and to discuss some clinical implications in reproductive endocrinology underscored by this expanding knowledge. DESIGN:Studies relating to follicular gro...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/s0015-0282(16)58077-7
更新日期：1996-02-01 00:00:00
