Abstract:
PURPOSE:Two noninvasive delivery strategies for VEGF/PDGF receptor tyrosine kinase inhibitors (RTKI) were explored that exploited uveal retention as a means for establishing an ocular drug depot: a single oral "loading" dose and topical administration. METHODS:Melanin binding was confirmed by centrifugation and mass spectrometry. Ocular retention was examined in pigmented and albino rats. Ocular release kinetics were measured 3 to 28 days postdosing in pigmented rats. Microautoradiography was used to demonstrate retention of RTKI in the uveal tract. A uveal drug depot of pazopanib was created by a single oral dose prior to induction of laser choroidal neovascularization (CNV). Choroid/retinal pigmented epithelium (RPE) retention of a related RTKI with enhanced topical bioavailability, GW771806, was confirmed by bioanalytics, and its ability to regress CNV compared with pazopanib. RESULTS:Pazopanib and GW771806 directly bound melanin and were retained within the uveal tract of pigmented rats for weeks following a single oral dose. Pazopanib was undetectable systemically following a single oral administration prior to CNV induction, and reduced CNV as well as twice daily dosing. Topical ocular delivery of GW771806 at 5 mg/mL led to high choroidal/RPE exposure and significantly regressed CNV lesions; 2 mg/mL prevented lesion progression. CONCLUSIONS:Uveal retention of drugs such as pazopanib can be used to create a sustained-release depot. Topical GW771806 regressed CNV. These data indicate that topical or infrequent oral loading dose treatment with VEGF/PDGF RTKI retained in the choroid/RPE might allow noninvasive treatments for ocular neovascular disease.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Robbie SJ,Lundh von Leithner P,Ju M,Lange CA,King AG,Adamson P,Lee D,Sychterz C,Coffey P,Ng YS,Bainbridge JW,Shima DTdoi
10.1167/iovs.12-10169subject
Has Abstractpub_date
2013-02-27 00:00:00pages
1490-500issue
2eissn
0146-0404issn
1552-5783pii
iovs.12-10169journal_volume
54pub_type
杂志文章abstract:PURPOSE:This study explored the role of the left posterior parietal cortex (PPC) in saccades, vergence, and combined saccade-vergence movements by means of transcranial magnetic stimulation (TMS) in humans. METHODS:TMS was applied over the left PPC for 80, 90, or 100 ms after target onset. In a control experiment, TMS...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.03-1291
更新日期:2004-07-01 00:00:00
abstract:PURPOSE:To investigate the pharmacologic effect of tetramethylpyrazine (TMP) on human Tenon's fibroblasts (HTFs), the cells implicated in scarring after filtration surgery. METHODS:Transforming growth factor-β2 (TGF-β2) was used to stimulate a fibrotic phenotype in primary HTFs, and the influence of TMP on the fibroti...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.16-19529
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:To design a contrast sensitivity perimetry (CSP) protocol that decreases variability in glaucomatous defects while maintaining good sensitivity to glaucomatous loss. METHODS:Twenty patients with glaucoma and 20 control subjects were tested with a CSP protocol implemented on a monitor-based testing station. In ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.07-1205
更新日期:2008-07-01 00:00:00
abstract::An analysis of size, shape, vacuole and cell counts of Schlemm's canal was undertaken in 20 human eyes that had undergone conventional immersion fixation. A reduction in vacuole count with age was shown to be associated with a decreased canal cell count. The decrease in cell count with age was closely linked to an age...
journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1990-04-01 00:00:00
abstract:PURPOSE:To characterize the possible role of transducin Gtβγ-complex in modulating the signaling properties of photoactivated rhodopsin and its lifetime in rod disc membranes and intact rods. METHODS:Rhodopsin photolysis was studied using UV-visible spectroscopy and rapid scanning spectroscopy in the presence of hydro...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-9350
更新日期:2012-03-09 00:00:00
abstract:PURPOSE:As efforts to noninvasively measure intracranial pressure (ICP) increase, we thought it important to investigate the timing of retinal venous pulsation in relation to the intraocular and intracranial pressure pulses. METHODS:Neurosurgical patients undergoing continuous direct ICP monitoring had video recording...
journal_title:Investigative ophthalmology & visual science
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更新日期:2012-07-10 00:00:00
abstract:PURPOSE:To determine whether a new cyclic GMP analog, 2'-O-(4-benzoyl)benzoylguanosine 3',5' cyclic monophosphate (BB-cGMP), significantly reduces intraocular pressure in either normotensive or hypertensive eyes of rabbits. METHODS:Intraocular pressure of normal adult rabbits and/or rabbits with alpha-chymotrypsin-ind...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract:Purpose:This study quantified the thickness and depth of the lamina cribrosa (LC) and peripapillary scleral thickness in high-resolution three-dimensional (3D) fluorescent reconstructions of the optic nerve head (ONH) in eyes from donors of African (AD) and European descent (ED). Methods:A total of 64 eyes (45 ED, 19 ...
journal_title:Investigative ophthalmology & visual science
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doi:10.1167/iovs.17-21842
更新日期:2017-07-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2018-07-02 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2013-01-17 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2019-07-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.09-4874
更新日期:2010-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
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更新日期:2020-02-07 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract:PURPOSE:To examine the expression of matrix metalloproteinases (MMPs) and their inhibitors during the development of retinal neovascularization (NV) in a mouse model. METHODS:A well-characterized murine model of retinal NV was used to study the expression of specific MMPs (MMP-2, MMP-9, and MT1-MMP) and tissue inhibit...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Long-term S-antigen (S-Ag) specific T lymphocyte lines can induce experimental autoimmune uveoretinitis (EAU) when transferred into naive rats systemically or intravitreally. The uveitogenic lymphocyte line (ThS) stains positively for the ART 18 (IL-2 receptor) and W3/25 (T helper/inducer); negatively for OX6 (RT1B) a...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:To characterize the cellular expression patterns of antiangiogenic factors differentially regulated in the fetal human macula. METHODS:RNA was extracted from macular, nasal, and surround biopsies of three human fetal retinas at midgestation. Relative levels of expression of pigment epithelium-derived factor (P...
journal_title:Investigative ophthalmology & visual science
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更新日期:2010-08-01 00:00:00
abstract::Interactions between direction-selective mechanisms for motion were examined in strabismic and anisometropic amblyopia. Amblyopes were found to have a marked reduction in sensitivity to flicker or movement when the amblyopic eye was presented with a combination of two gratings drifting in opposite directions at the sa...
journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1980-09-01 00:00:00
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更新日期:2006-11-01 00:00:00
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更新日期:2008-07-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1997-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2013-06-12 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2011-07-15 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2013-06-06 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2006-06-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00