Abstract:
:Long-term S-antigen (S-Ag) specific T lymphocyte lines can induce experimental autoimmune uveoretinitis (EAU) when transferred into naive rats systemically or intravitreally. The uveitogenic lymphocyte line (ThS) stains positively for the ART 18 (IL-2 receptor) and W3/25 (T helper/inducer); negatively for OX6 (RT1B) and OX8 (T suppressor/cytotoxic cells). The inflammation induced by systemic or intravitreal transfer of the ThS line was studied with respect to the surface markers of the infiltrating cells, as well as the markers expressed by the resident ocular cells, by immunohistopathological techniques. On day 4 to 5 after systemic injection of the ThS line, rare T cells (W3/25+, ART 18+) and macrophages (OX42+, OX6+) surrounded by some resident cells that expressed MHC class II antigens were identified in the ciliary body and choroid. Shortly thereafter (10-20 hr) more macrophages, polymorphonuclear leukocytes, and T lymphocytes (W3/25+, then OX8+) appeared in the inflamed eye. The kinetics was similar to EAU induced by active immunization. The rats with severe disease expressed the MHC class II antigens on large numbers of resident cells in the eye. Intravitreally transferred ThS cells migrated to the retina within 24 hr of transfer. Infiltration of macrophages (OX6+, OX42+) and other T lymphocytes (OX6+, W3/25+ or OX8+), in conjunction with photoreceptor damage, were observed within the next 24-48 hr. The cells with the ThS markers disappeared from the eye on day 4-5 post transfer. These findings suggest that the ThS line can recognize the photoreceptor S-Ag in vivo.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Chan CC,Caspi RR,Roberge FG,Nussenblatt RBsubject
Has Abstractpub_date
1988-03-01 00:00:00pages
411-8issue
3eissn
0146-0404issn
1552-5783journal_volume
29pub_type
杂志文章abstract:PURPOSE:Cortical cataract in humans is associated with Ca2+ overload and protein loss, and although animal models of cataract have implicated Ca2+-activated proteases in this process, it remains to be determined whether the human lens responds in this manner to conditions of Ca2+ overload. The purpose of these experime...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract:PURPOSE:To determine the effects of overexpression of mutated BIGH3 in HeLa and human corneal epithelial (HCE) cells. METHODS:Six mutations known to be responsible for autosomal dominant corneal dystrophies linked to chromosome 5 were generated in a BIGH3 expression vector and transfected in HeLa and HCE cells. The ex...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-0661
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:Our study aimed to explore the heritability of retinal vascular caliber among Singapore Chinese families. METHODS:In the Strabismus, Amblyopia, and Refractive Error Study in Singaporean Chinese Preschoolers (STARS) family study conducted from 2008 to 2010, a total of 727 participants (304 parent-child pairs, 8...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-12418
更新日期:2013-08-19 00:00:00
abstract:Purpose:To evaluate the association between endothelial cell density (ECD) after Descemet's stripping automated endothelial keratoplasty (DSAEK) and preoperative cytokine levels in the aqueous humor (AqH). Methods:This prospective consecutive case series included 97 consecutive patients who underwent DSAEK (64 eyes) o...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.17-23049
更新日期:2018-02-01 00:00:00
abstract::The effect of systemic hypoxia on the maintained firing rate (MFR) of single retinal ganglion cells has been measured in the cat at a constant luminance level. Systemic hypoxia was produced by reducing the percentage of oxygen in the respiratory mixture under forced ventilation so that arterial PCO2 and pH were consta...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract:PURPOSE:To identify major gangliosides - the sialated glycolipids - of corneal epithelium; to determine which specific gangliosides, if any, are synthesized in a higher amount or are downregulated during corneal epithelial cell migration; and to determine what role, if any, they play in the modulation of corneal epithe...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1996-03-01 00:00:00
abstract:PURPOSE:Recoverin has been identified as a target autoantigen for antirecoverin antibodies found in the sera of some patients with cancer-associated retinopathy. The aim of this study was to investigate the role of antirecoverin antibodies in cancer-associated retinopathy. METHODS:Human, rat, and rabbit antirecoverin ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract:PURPOSE:To identify genes and molecular mechanisms associated with photoreceptor degeneration in a canine model of XLRP caused by an RPGR exon ORF15 microdeletion. Methods. Expression profiles of mutant and normal retinas were compared by using canine retinal custom cDNA microarrays. qRT-PCR, Western blot analysis, and...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.10-5443
更新日期:2010-11-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-13093
更新日期:2013-12-17 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-8763
更新日期:2012-01-10 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.19-26749
更新日期:2019-06-03 00:00:00
abstract:PURPOSE:RPE65 has been shown to be essential for the production of 11-cis retinal by the retinal pigment epithelium. Mutations in RPE65 are known to be associated with severe forms of early-onset retinal dystrophy. This project was designed to determine the amount of regenerable opsin in Rpe65-/- mice during developmen...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-0567
更新日期:2003-01-01 00:00:00
abstract:PURPOSE:To assess the diagnostic validity of a fully automated image analysis algorithm of in vivo confocal microscopy images in quantifying corneal subbasal nerves to diagnose diabetic neuropathy. METHODS:One hundred eighty-six patients with type 1 and type 2 diabetes mellitus (T1/T2DM) and 55 age-matched controls un...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.13-13787
更新日期:2014-04-03 00:00:00
abstract:PURPOSE:Previous sequence analyses of hemidesmosomal BP 180/collagen XVII cDNA from human skin and of a similar chicken corneal cDNA showed some similarities, but major differences as well. The authors examined whether, in one species, the same mRNA is present in cornea and skin. They also studied the developmental exp...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:Purpose:We assess if α retinal ganglion cells (αRGCs) and intrinsically photosensitive retinal ganglion cells (ipRGCs) survive in mouse models of glaucoma. Methods:Two microliters of N-methyl-D-aspartate (NMDA; 1 mM) or PBS were injected intraocularly 7 days before sacrifice. Immunohistochemical analyses of the retina...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.19-27145
更新日期:2019-09-03 00:00:00
abstract:PURPOSE:Recently, a segregation study in families with uveal and cutaneous melanoma identified 9q21 as a potential locus harboring a tumor-suppressor gene (TSG). One of the genes in this area, RASEF, was then analyzed as a candidate TSG, but lack of point mutations and copy number changes could not confirm this. In thi...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.07-1135
更新日期:2008-04-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.02-0980
更新日期:2003-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.11-7774
更新日期:2011-10-11 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,随机对照试验
doi:10.1167/iovs.15-17786
更新日期:2015-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:To investigate the nature and reversibility of biochemical and functional changes in the retina encountered over a single generation of dietary n-3 polyunsaturated fatty acid deficiency in guinea pigs. METHODS:Dunkin-Hartley guinea pigs were fed for 16 weeks after weaning with diets supplemented with safflower...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1999-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.61.12.12
更新日期:2020-10-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1987-05-01 00:00:00
abstract::Five immunosuppressive and anti-inflammatory agents were tested for their effects on development of experimental autoimmune uveoretinitis (EAU) and immune responses to S-antigen in rats immunized with this retinal antigen. When administered daily from day 0-14 after immunization, cyclosporine at 5-20 mg/Kg was nontoxi...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract:PURPOSE:Na,K-adenosine triphosphatase (ATPase) is essential for the regulation of cytoplasmic ion concentrations in lens cells. Earlier studies demonstrated that tyrosine phosphorylation by Lyn kinase, a Src-family member, inhibits Na,K-ATPase activity in porcine lens epithelium. In the present study, experiments were ...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:10.1167/iovs.04-0809
更新日期:2005-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
doi:10.1167/iovs.14-15523
更新日期:2014-12-18 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::The entoptic phenomenon by which one can observe leukocytes flowing in one's own parafoveal capillaries has been used to study the effect of changes in perfusion pressure on blood flow. Our measurements in humans with normal ocular fundi indicate that the retinal circulation of the parafovea is autoregulated in relati...
journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00