Lipid protein interactions in mitochondria. VIII. Effect of general anesthetics on the mobility of spin labels in lipid vesicles and mitochondrial membranes.

abstract：:The past 5 years has witnessed an advance in our understanding of alpha1G (Cav3.1), alpha1H (Cav3.2), and alpha11 (Car3.3), the pore-forming subunits of T-type or low-voltage-activated calcium channels (LVAs). LVAs differ in their localization and molecular, biophysical, and biochemical properties, but all conduct a t...

journal_title：Journal of bioenergetics and biomembranes

authors： Yunker AM,McEnery MW

更新日期：2003-12-01 00:00:00

abstract：:Calcium may function directly in several aspects of photosynthesis. It appears to modulate activity of the phosphatase enzymes in the carbon reduction cycle and also to regulate chloroplast NAD+ kinase activity through a calmodulin-like protein. Some evidence supports a calcium function in the water-splitting complex,...

journal_title：Journal of bioenergetics and biomembranes

authors： Brand JJ,Becker DW

更新日期：1984-08-01 00:00:00

abstract：:Traditionally, mitochondria have been viewed as the "powerhouse" of the cell, i.e., the site of the oxidative phosphorylation machinery involved in ATP production. Consequently, much of the research conducted on mitochondria over the past 4 decades has focused on elucidating both those molecular events involved in ATP...

journal_title：Journal of bioenergetics and biomembranes

authors： Pedersen PL

更新日期：1999-08-01 00:00:00

abstract：:Eukaryotic porins or VDACs are a class of transmembrane proteins mainly localized in the outer mitochondrial membrane, whose function is to allow the diffusion of metabolites between the cytosol and the mitochondrion. In Drosophila melanogaster, as in other organisms, a small family of genes encoding porins has been d...

journal_title：Journal of bioenergetics and biomembranes

authors： Specchia V,Guarino F,Messina A,Bozzetti MP,De Pinto V

更新日期：2008-06-01 00:00:00

abstract：:Many oxidoreductases are constructed from (a) local sites of strongly coupled substrate-redox cofactor partners participating in exchange of electron pairs, (b) electron pair/single electron transducing redox centers, and (c) nonadiabatic, long-distance, single-electron tunneling between weakly coupled redox centers. ...

journal_title：Journal of bioenergetics and biomembranes

authors： Moser CC,Page CC,Farid R,Dutton PL

更新日期：1995-06-01 00:00:00

abstract：:Relevant Ca(2+) pools and fluxes in H9c2 cells have been studied using fluorescent indicators and Ca(2+)-mobilizing agents. Vasopressin produced a cytoplasmic Ca(2+) peak with half-maximal effective concentration of 6 nM, whereas thapsigargin-induced Ca(2+) increase showed half-maximal effect at 3 nM. Depolarization o...

journal_title：Journal of bioenergetics and biomembranes

authors： Lax A,Soler F,Fernández-Belda F

更新日期：2005-08-01 00:00:00

abstract：:Thylakoids are highly protein-enriched membranes that harbor a number of multicomponent photosynthetic complexes. Similarly to other biological membranes the protein constituents are heterogeneously distributed laterally in the plane of the membrane, however the specific segregation into stacked (grana patches) and un...

journal_title：Journal of bioenergetics and biomembranes

authors： Petrova N,Todinova S,Paunov M,Kovács L,Taneva S,Krumova S

更新日期：2018-12-01 00:00:00

abstract：:The term "direct-coupled" is considered in the context of redox-linked proton translocation mechanisms, and the origins of this concept, its philosophical implications, applications, and contributions to the development of bioenergetics, are discussed. ...

journal_title：Journal of bioenergetics and biomembranes

authors： West IC

更新日期：1991-10-01 00:00:00

abstract：:Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused ...

journal_title：Journal of bioenergetics and biomembranes

authors： Benz R,Schmid A,Dihanich M

更新日期：1989-08-01 00:00:00

abstract：:Porin or voltage-dependent anion-selective channel (VDAC) is the main protein responsible for the high permeability of the outer mitochondrial membrane. The mitochondrial porin is mainly composed of sided beta-strands, in analogy with bacterial porin, whose structure has been resolved at 1.8 A resolution. In mitochond...

journal_title：Journal of bioenergetics and biomembranes

authors： De Pinto VD,Palmieri F

更新日期：1992-02-01 00:00:00

abstract：:It is proven that any model of localized protonmotive energy coupling that relies upon properties of a homogeneous surface phase must, when operated in the steady state, lead to bulk phase electrochemical potentials for protons that are as large as those required by the delocalized chemiosmotic theory. To obtain model...

journal_title：Journal of bioenergetics and biomembranes

authors： Nagle JF,Dilley RA

更新日期：1986-02-01 00:00:00

abstract：:Beef heart cytochrome c oxidase was reconstituted in asolectin liposomes containing the pH indicator fluorescein-phosphatidylethanolamine (FPE) by the cholate-dialysis procedure. The influence of FPE on the asolectin liposome size and of the removal of subunit III from the complex on its incorporation into liposomes w...

journal_title：Journal of bioenergetics and biomembranes

authors： Müller M,Azzi A

更新日期：1985-12-01 00:00:00

abstract：:The P515 absorbance change upon single-turnover light flashes has been studied in intact leaves and isolated chloroplasts from spinach. A comparative study of the effects of preillumination on the kinetics of the P515 response and on the activity of the chloroplast ATPase has been made. The slow component (reaction 2)...

journal_title：Journal of bioenergetics and biomembranes

authors： Peters RL,Bossen M,van Kooten O,Vredenberg WJ

更新日期：1983-12-01 00:00:00

abstract：:This introductory article and those that follow focus on the roles that mitochondria may have in cancer metastasis (spreading) that all too frequently leads to death of cancer patients. The history of cancer dates back in time to several thousand years BC and continues to this day. Although billions of dollars have be...

journal_title：Journal of bioenergetics and biomembranes

authors： Pedersen PL

更新日期：2012-12-01 00:00:00

abstract：:Proton transport-coupled unisite catalysis was measured with the H(+)-ATPase from chloroplasts. The reaction was measured in the ATP hydrolysis direction under deenergized conditions and in the ATP synthesis direction under energized conditions. The equilibrium constant of the enzyme does not change upon energization,...

journal_title：Journal of bioenergetics and biomembranes

authors： Gräber P,Labahn A

更新日期：1992-10-01 00:00:00

abstract：:The present state of the chemiosmotic concept is reviewed. Special attention is paid to (i) further progress in studies on the Na(+)-coupled energetics and (ii) paradoxical bioenergetic effects when protonic or sodium potentials are utilized outside the coupling membrane (TonB-mediated uphill transports across the out...

journal_title：Journal of bioenergetics and biomembranes

authors： Skulachev VP

更新日期：1994-12-01 00:00:00

abstract：:Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) has been recently identified as a novel inhibitor of multiple tumors; however, its role in glioblastoma (GBM) has not been investigated. This study aimed to evaluate whether LHPP exerts a potential tumor-inhibiting role in GBM. Compared with tha...

journal_title：Journal of bioenergetics and biomembranes

authors： Li C,Yang J,Wang W,Li R

更新日期：2021-02-01 00:00:00

abstract：:The electrolyte-pigment-metal system can be described as analogous to a photosensitive junction region. When the thickness of the pigment film is increased, the action spectra of the maximum short-circuit photo-current under continuous illumination differ from the absorption spectra both in direct illumination (pigmen...

journal_title：Journal of bioenergetics and biomembranes

authors： Villar JG

更新日期：1976-08-01 00:00:00

abstract：:ATP binding cassette (ABC) transporters comprise an extended protein family involved in the transport of a broad spectrum of solutes across membranes. They consist of a common architecture including two ATP-binding domains converting chemical energy into conformational changes and two transmembrane domains facilitatin...

journal_title：Journal of bioenergetics and biomembranes

authors： Nikles D,Tampé R

更新日期：2007-12-01 00:00:00

abstract：:A rapid loss of accumulated Ca2+ is produced by addition of H+ to isolated heart mitochondria. The H+-dependent Ca+ efflux requires that either (a) the NAD(P)H pool of the mitochondrion be oxidized, or (b) the endogenous adenine nucleotides be depleted. The loss of Ca2+ is accompanied by swelling and loss of endogenou...

journal_title：Journal of bioenergetics and biomembranes

authors： Jurkowitz MS,Brierley GP

更新日期：1982-12-01 00:00:00

abstract：:A great deal of progress has been made in understanding both the structure and the mechanism of F1-ATPase. The primary structure is now fully known for at least five species. Sequence comparison between chloroplast, photobacteria, aerobic bacteria, and mitochondrial representatives allow us to infer more general funct...

journal_title：Journal of bioenergetics and biomembranes

authors： Ysern X,Amzel LM,Pedersen PL

更新日期：1988-08-01 00:00:00

abstract：:Mitochondrial impairment has been collecting more and more attention as a contributing factor to the etiology of Parkinson's disease. Above all, the NADH-quinone oxidoreductase, complex I, of the respiratory chain seems to be most culpable. Complex I dysfunction is translated to an increased production of reactive oxy...

journal_title：Journal of bioenergetics and biomembranes

authors： Marella M,Seo BB,Yagi T,Matsuno-Yagi A

更新日期：2009-12-01 00:00:00

abstract：:The ATP synthase of the yeast Saccharomyces cerevisiae is composed of 20 different subunits whose primary structure is known. The organization of proteins that constitute the membranous domain is now under investigation. Cysteine insertions combined with the use of nonpermeant maleimide reagents and cross-linking reag...

journal_title：Journal of bioenergetics and biomembranes

authors： Velours J,Arselin G

更新日期：2000-08-01 00:00:00

abstract：:For the past seven years or so, much discussion and controversy in the field of human evolution has revolved around the application and interpretation of studies of human mitochondrial DNA variation, particularly the hypothesis that all mtDNA types in contemporary populations can be traced back to a single African anc...

journal_title：Journal of bioenergetics and biomembranes

authors： Stoneking M

更新日期：1994-06-01 00:00:00

abstract：:Signal peptidases, the endoproteases that remove the amino-terminal signal sequence from many secretory proteins, have been isolated from various sources. Seven signal peptidases have been purified, two from E. coli, two from mammalian sources, and three from mitochondrial matrix. The mitochondrial enzymes are soluble...

journal_title：Journal of bioenergetics and biomembranes

authors： Dev IK,Ray PH

更新日期：1990-06-01 00:00:00

abstract：:The induction of millisecond delayed fluorescence mediated by PS I-dependent proton pumping has been used as an indicator of the time course with which those protons equilibrate with sites on the oxygen-evolving enzyme complex (Bowes, J. M., and Crofts, A. R. (1978). Z. Naturforsch. 33C, 271-275). We found that the in...

journal_title：Journal of bioenergetics and biomembranes

authors： Theg SM,Belanger KM,Dilley RA

更新日期：1987-02-01 00:00:00

abstract：:Mitochondrial cytochrome c (horse), which is a very efficient electron donor to bacterial photosynthetic reaction centers in vitro, binds to the reaction center of Rhodospirillum rubrum with an approximate dissociation constant of 0.3-0.5 microM at pH 8.2 and low ionic strength. The binding site for the reaction cente...

journal_title：Journal of bioenergetics and biomembranes

authors： Bosshard HR,Snozzi M,Bachofen R

更新日期：1987-08-01 00:00:00

abstract：:Cytochrome c oxidase was purified from mitochondria of Euglena gracilis and separated into 15 different polypeptide subunits by polyacrylamide gel electrophoresis. All 15 subunits copurify through various purification procedures, and the subunit composition of the isolated enzyme is identical to that of the immunoprec...

journal_title：Journal of bioenergetics and biomembranes

authors： Brönstrup U,Hachtel W

更新日期：1989-06-01 00:00:00

abstract：:The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. For this purpose, a rapid and sensitive method was developed that e...

journal_title：Journal of bioenergetics and biomembranes

authors： Klepinin A,Ounpuu L,Guzun R,Chekulayev V,Timohhina N,Tepp K,Shevchuk I,Schlattner U,Kaambre T

更新日期：2016-10-01 00:00:00

abstract：:Crude membrane preparations of a rho0 mutant of Saccharomyces cerevisiae exhibit Mg2+-dependent ATPase activity. Over the optimal pH range, 5.0-6.75, the apparent Vmax of the enzyme equals 590 nmoles of ATP hydrolyzed per minute per milligram protein, with an apparent Km for ATP of 1.3 mM. ATP hydrolysis is insensitiv...

journal_title：Journal of bioenergetics and biomembranes

authors： McDonough JP,Jaynes PK,Mahler HR

更新日期：1980-08-01 00:00:00