当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Depletion of cellular polyamines, spermidine and spermine, causes a total arrest in translation and growth in mammalian cells.

Depletion of cellular polyamines, spermidine and spermine, causes a total arrest in translation and growth in mammalian cells.

Abstract：

:The polyamines, putrescine, spermidine, and spermine, are essential polycations, intimately involved in the regulation of cellular proliferation. Although polyamines exert dynamic effects on the conformation of nucleic acids and macromolecular synthesis in vitro, their specific functions in vivo are poorly understood. We investigated the cellular function of polyamines by overexpression of a key catabolic enzyme, spermidine/spermine N(1)-acetyltransferase 1 (SAT1) in mammalian cells. Transient cotransfection of HeLa cells with GFP and SAT1 vectors suppressed GFP protein expression without lowering its mRNA level, an indication that the block in GFP expression was not at transcription, but at translation. Fluorescence single-cell imaging also revealed specific inhibition of endogenous protein synthesis in the SAT1 overexpressing cells, without any inhibition of synthesis of DNA or RNA. Overexpression of SAT1 using a SAT1 adenovirus led to rapid depletion of cellular spermidine and spermine, total inhibition of protein synthesis, and growth arrest within 24 h. The SAT1 effect is most likely due to depletion of spermidine and spermine, because stable polyamine analogs that are not substrates for SAT1 restored GFP and endogenous protein synthesis. Loss of polysomes with increased 80S monosomes in the polyamine-depleted cells suggests a direct role for polyamines in translation initiation. Our data provide strong evidence for a primary function of polyamines, spermidine and spermine, in translation in mammalian cells.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Mandal S,Mandal A,Johansson HE,Orjalo AV,Park MH

doi

10.1073/pnas.1219002110

subject

Has Abstract

pub_date

2013-02-05 00:00:00

pages

2169-74

issue

6

eissn

0027-8424

issn

1091-6490

pii

1219002110

journal_volume

110

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Crystal structures of Mmm1 and Mdm12-Mmm1 reveal mechanistic insight into phospholipid trafficking at ER-mitochondria contact sites.

    abstract：:The endoplasmic reticulum (ER)-mitochondria encounter structure (ERMES) comprises mitochondrial distribution and morphology 12 (Mdm12), maintenance of mitochondrial morphology 1 (Mmm1), Mdm34, and Mdm10 and mediates physical membrane contact sites and nonvesicular lipid trafficking between the ER and mitochondria in y...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1715592114

    authors： Jeong H,Park J,Jun Y,Lee C

    更新日期：2017-11-07 00:00:00

  • Hypersensitive site 6 of the Th2 locus control region is essential for Th2 cytokine expression.

    abstract：:The T helper type 2 (Th2) cytokine genes Il4, Il5, and Il13 are contained within a 140-kb region of mouse chromosome 11 and their expression is controlled by a locus control region (LCR) embedded within this locus. The LCR is composed of a number of DNase I-hypersensitive sites (HSs), which are believed to encompass t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1304720110

    authors： Williams A,Lee GR,Spilianakis CG,Hwang SS,Eisenbarth SC,Flavell RA

    更新日期：2013-04-23 00:00:00

  • Related cell-surface antigens expressed with positional specificity in Drosophila imaginal discs.

    abstract：:We have isolated three classes of monoclonal antibodies against Drosophila cell-surface antigens that are expressed with positional specificity in imaginal discs. Comparison of immunofluorescence patterns with the wing-disc fate map reveals that expression of the antigens is not directly related to the specific type o...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.23.7485

    authors： Brower DL,Wilcox M,Piovant M,Smith RJ,Reger LA

    更新日期：1984-12-01 00:00:00

  • Three-dimensional scroll waves organize Dictyostelium slugs.

    abstract：:To test the hypothesis that periodic signals and chemotaxis direct later morphogenesis in Dictyostelium discoideum, we investigated cell behavior and cell movement in slugs. Trails of neutral red-stained prestalk and anterior-like cells were recorded by high-resolution digital image processing. Neutral red-stained ant...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.14.6433

    authors： Siegert F,Weijer CJ

    更新日期：1992-07-15 00:00:00

  • Organization of four mouse lambda light chain immunoglobulin genes.

    abstract：:We have cloned four lambda light chain constant region (C) genes from mouse embryo DNA. Each carries its own joining (J) segment approximately 1.3 kilobases to its 5' side. The four C genes occur in two clusters, 5' J3C3J1C13' and 5' J2C2J4C43', with C4 being a new C lambda gene. We have also shown that V lambda 1 is ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.6.3765

    authors： Blomberg B,Traunecker A,Eisen H,Tonegawa S

    更新日期：1981-06-01 00:00:00

  • Failure of the opiate antagonist naloxone to modify hypnotic analgesia.

    abstract：:Hypnotic analgesia in some respects resembles opiate analgesia. We tested the hypothesis that some features of hypnotic analgesia are mediated through neuronal pathways activating specific opiate receptors in brain. The opiate antagonist naloxone had no effect on hypnotic analgesia in three subjects. Thus, the hypothe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.6.2041

    authors： Goldstein A,Hilgard ER

    更新日期：1975-06-01 00:00:00

  • The age-specific force of natural selection and biodemographic walls of death.

    abstract：:W. D. Hamilton's celebrated formula for the age-specific force of natural selection furnishes predictions for senescent mortality due to mutation accumulation, at the price of reliance on a linear approximation. Applying to Hamilton's setting the full nonlinear demographic model for mutation accumulation recently deve...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1306656110

    authors： Wachter KW,Evans SN,Steinsaltz D

    更新日期：2013-06-18 00:00:00

  • Cooperative interactions between the interleukin 2 receptor alpha and beta chains alter the interleukin 2-binding affinity of the receptor subunits.

    abstract：:The interleukin 2 (IL-2) receptor (IL-2R) is a multisubunit receptor that includes three major IL-2 binding subunits, the IL-2R alpha, beta, and gamma chains. We have detected and analyzed cooperative interactions between the IL-2R alpha and beta chains (IL-2R alpha and IL-2R beta, respectively) in COS cells transfect...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.8.3344

    authors： Roessler E,Grant A,Ju G,Tsudo M,Sugamura K,Waldmann TA

    更新日期：1994-04-12 00:00:00

  • Nuclear dynamics in a fungal chimera.

    abstract：:A fungal colony is a syncytium composed of a branched and interconnected network of cells. Chimerism endows colonies with increased virulence and ability to exploit nutritionally complex substrates. Moreover, chimera formation may be a driver for diversification at the species level by allowing lateral gene transfer b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1220842110

    authors： Roper M,Simonin A,Hickey PC,Leeder A,Glass NL

    更新日期：2013-08-06 00:00:00

  • Localization of virus-specific and group-specific epitopes of plant potyviruses by systematic immunochemical analysis of overlapping peptide fragments.

    abstract：:Virus-specific or group-specific antibody probes to potyviruses can be produced by targeting the immune response to the virus-specific, N-terminal region of the capsid protein (29-95 amino acids depending on the virus) or to the conserved core region (216 amino acids) of the capsid protein, respectively. Immunochemica...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.21.8192

    authors： Shukla DD,Tribbick G,Mason TJ,Hewish DR,Geysen HM,Ward CW

    更新日期：1989-11-01 00:00:00

  • Leptin as a modulator of sweet taste sensitivities in mice.

    abstract：:Leptin acts as a potent inhibitory factor against obesity by regulating energy expenditure, food intake, and adiposity. The obese diabetic db/db mouse, which has defects in leptin receptor, displays enhanced neural responses and elevated behavioral preference to sweet stimuli. Here, we show the effects of leptin on th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.190066697

    authors： Kawai K,Sugimoto K,Nakashima K,Miura H,Ninomiya Y

    更新日期：2000-09-26 00:00:00

  • Mild mitochondrial uncoupling impacts cellular aging in human muscles in vivo.

    abstract：:Faster aging is predicted in more active tissues and animals because of greater reactive oxygen species generation. Yet age-related cell loss is greater in less active cell types, such as type II muscle fibers. Mitochondrial uncoupling has been proposed as a mechanism that reduces reactive oxygen species production an...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0610131104

    authors： Amara CE,Shankland EG,Jubrias SA,Marcinek DJ,Kushmerick MJ,Conley KE

    更新日期：2007-01-16 00:00:00

  • Genetic analysis of a bacterial genetic exchange element: the gene transfer agent of Rhodobacter capsulatus.

    abstract：:An unusual system of genetic exchange exists in the purple nonsulfur bacterium Rhodobacter capsulatus. DNA transmission is mediated by a small bacteriophage-like particle called the gene transfer agent (GTA) that transfers random 4.5-kb segments of the producing cell's genome to recipient cells, where allelic replacem...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.2.859

    authors： Lang AS,Beatty JT

    更新日期：2000-01-18 00:00:00

  • Listener evaluations of new and Old Italian violins.

    abstract：:Old Italian violins are routinely credited with playing qualities supposedly unobtainable in new instruments. These qualities include the ability to project their sound more effectively in a concert hall-despite seeming relatively quiet under the ear of the player-compared with new violins. Although researchers have l...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1619443114

    authors： Fritz C,Curtin J,Poitevineau J,Tao FC

    更新日期：2017-05-23 00:00:00

  • Human herpesvirus-6 entry into the central nervous system through the olfactory pathway.

    abstract：:Viruses have been implicated in the development of neurodegenerative diseases, such as Alzheimer's, Parkinson's, and multiple sclerosis. Human herpesvirus-6 (HHV-6) is a neurotropic virus that has been associated with a wide variety of neurologic disorders, including encephalitis, mesial temporal lobe epilepsy, and mu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1105143108

    authors： Harberts E,Yao K,Wohler JE,Maric D,Ohayon J,Henkin R,Jacobson S

    更新日期：2011-08-16 00:00:00

  • Short amino acid stretches can mediate amyloid formation in globular proteins: the Src homology 3 (SH3) case.

    abstract：:Protein misfolding and deposition underlie an increasing number of debilitating human disorders. We have shown that model proteins unrelated to disease, such as the Src homology 3 (SH3) domain of the p58alpha subunit of bovine phosphatidyl-inositol-3'-kinase (PI3-SH3), can be converted in vitro into assemblies with st...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0308249101

    authors： Ventura S,Zurdo J,Narayanan S,Parreño M,Mangues R,Reif B,Chiti F,Giannoni E,Dobson CM,Aviles FX,Serrano L

    更新日期：2004-05-11 00:00:00

  • Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells.

    abstract：:T cells interacting with antigen-presenting cells (APCs) form an "immunological synapse" (IS), a bull's-eye pattern composed of a central supramolecular activation cluster enriched with T cell receptors (TCRs) surrounded by a ring of adhesion molecules (a peripheral supramolecular activation cluster). The mechanism re...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710258105

    authors： Kaizuka Y,Douglass AD,Varma R,Dustin ML,Vale RD

    更新日期：2007-12-18 00:00:00

  • Assembly and mechanism of a group II ECF transporter.

    abstract：:Energy-coupling factor (ECF) transporters are a recently discovered family of primary active transporters for micronutrients and vitamins, such as biotin, thiamine, and riboflavin. Found exclusively in archaea and bacteria, including the human pathogens Listeria, Streptococcus, and Staphylococcus, ECF transporters may...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1217361110

    authors： Karpowich NK,Wang DN

    更新日期：2013-02-12 00:00:00

  • Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein.

    abstract：:A hybrid bacteriophage, P420, was constructed in vitro; it contains part of bacteriophage P4 and a 3.6-kilobase derivative of plasmid ColE1. In Escherichia coli, the plasmid-phage hybrid can exist as a stable plasmid or can be packaged into infective bacteriophage particles. Replication of P420, directed by the ColE1 ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.5.2200

    authors： Kahn M,Helinski DR

    更新日期：1978-05-01 00:00:00

  • Resting state of the human proton channel dimer in a lipid bilayer.

    abstract：:The voltage-gated proton channel Hv1 plays a critical role in the fast proton translocation that underlies a wide range of physiological functions, including the phagocytic respiratory burst, sperm motility, apoptosis, and metastatic cancer. Both voltage activation and proton conduction are carried out by a voltage-se...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1515043112

    authors： Li Q,Shen R,Treger JS,Wanderling SS,Milewski W,Siwowska K,Bezanilla F,Perozo E

    更新日期：2015-11-03 00:00:00

  • Functional map and domain structure of MET, the product of the c-met protooncogene and receptor for hepatocyte growth factor/scatter factor.

    abstract：:Little is known about the large ectodomain of MET, the product of the c-met protooncogene and receptor for hepatocyte growth factor/scatter factor (HGF/SF). Here, we establish by deletion mutagenesis that the HGF/SF and heparin-binding sites of MET are contained within a large N-terminal domain spanning the alpha-chai...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2034936100

    authors： Gherardi E,Youles ME,Miguel RN,Blundell TL,Iamele L,Gough J,Bandyopadhyay A,Hartmann G,Butler PJ

    更新日期：2003-10-14 00:00:00

  • Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ.

    abstract：:We demonstrate that individual cells infected with and expressing a recombinant retrovirus carrying the Escherichia coli beta-galactosidase gene (lacZ) can be viably stained, analyzed, sorted, and cloned by fluorescence-activated cell sorting based on the levels of lacZ expressed. To accomplish this we have devised a ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.8.2603

    authors： Nolan GP,Fiering S,Nicolas JF,Herzenberg LA

    更新日期：1988-04-01 00:00:00

  • Kinetic isotope effects in Ras-catalyzed GTP hydrolysis: evidence for a loose transition state.

    abstract：:A remote labeling method has been developed to determine (18)O kinetic isotope effects (KIEs) in Ras-catalyzed GTP hydrolysis. Substrate mixtures consist of (13)C-depleted GTP and [(18)O,(13)C]GTP that contains (18)O at phosphoryl positions of mechanistic interest and (13)C at all carbon positions of the guanosine moi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0401675101

    authors： Du X,Black GE,Lecchi P,Abramson FP,Sprang SR

    更新日期：2004-06-15 00:00:00

  • Structural origins of adenine-tract bending.

    abstract：:DNA sequences containing short adenine tracts are intrinsically curved and play a role in transcriptional regulation. Despite many high-resolution NMR and x-ray studies, the origins of curvature remain disputed. Long-range restraints provided by 85 residual dipolar couplings were measured for a DNA decamer containing ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0437877100

    authors： Barbic A,Zimmer DP,Crothers DM

    更新日期：2003-03-04 00:00:00

  • Purification of a terminal uridylyltransferase that acts as host factor in the in vitro poliovirus replicase reaction.

    abstract：:Poliovirus RNA polymerase requires a host factor to initiate RNA synthesis in vitro. The host factor was previously purified to near homogeneity from HeLa cells but was not assigned an enzymatic activity. This report describes the purification of a terminal uridylyltransferase that can act as host factor. By all crite...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.2.221

    authors： Andrews NC,Baltimore D

    更新日期：1986-01-01 00:00:00

  • A role for the actin-bundling protein L-plastin in the regulation of leukocyte integrin function.

    abstract：:Regulation of leukocyte integrin avidity is a crucial aspect of inflammation and immunity. The actin cytoskeleton has an important role in the regulation of integrin function, but the cytoskeletal proteins involved are largely unknown. Because inflammatory stimuli that activate integrin-mediated adhesion in human poly...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.16.9331

    authors： Jones SL,Wang J,Turck CW,Brown EJ

    更新日期：1998-08-04 00:00:00

  • Tunicamycin blocks neuritogenesis and glucosamine labeling of gangliosides in developing cerebral neuron cultures.

    abstract：:Fetal cerebral neurons at the initiation of active neurite outgrowth in culture incorporate 4-fold more [3H]glucosamine into glycoproteins than into the cellular lipid fraction. After 8 days or longer, when a well-developed fiber network is apparent, lipids are labeled more extensively than the glycoproteins. Labeling...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.18.5638

    authors： Yavin E,Richter-Landsberg C,Duksin D,Yavin Z

    更新日期：1984-09-01 00:00:00

  • The crystal structure of human interferon beta at 2.2-A resolution.

    abstract：:Type I interferons (IFNs) are helical cytokines that have diverse biological activities despite the fact that they appear to interact with the same receptor system. To achieve a better understanding of the structural basis for the different activities of alpha and beta IFNs, we have determined the crystal structure of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.22.11813

    authors： Karpusas M,Nolte M,Benton CB,Meier W,Lipscomb WN,Goelz S

    更新日期：1997-10-28 00:00:00

  • Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide.

    abstract：:Escherichia coli lipopolysaccharide (LPS)-induced nitrate biosynthesis was studied in LPS-sensitive C3H/He and LPS-resistant C3H/HeJ mice. Intraperitoneal injection of 15 micrograms of LPS led to a temporary 5- to 6-fold increase in blood nitrate concentration in the C3H/He strain. Levels of nitrate excreted in the ur...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.22.7738

    authors： Stuehr DJ,Marletta MA

    更新日期：1985-11-01 00:00:00

  • Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence.

    abstract：:We studied the coupled binding and folding of alpha-synuclein, an intrinsically disordered protein linked with Parkinson's disease. Using single-molecule fluorescence resonance energy transfer and correlation methods, we directly probed protein membrane association, structural distributions, and dynamics. Results reve...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0809232106

    authors： Ferreon AC,Gambin Y,Lemke EA,Deniz AA

    更新日期：2009-04-07 00:00:00