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Profiling the proteome of exhaled breath condensate in healthy smokers and COPD patients by LC-MS/MS.

Abstract：

:Three pools of exhaled breath condensate (EBC) from non-smokers plus healthy smokers (NS + HS, n = 45); chronic obstructive pulmonary disease (COPD) without emphysema (COPD, n = 15) and subjects with pulmonary emphysema associated with α(1)-antitrypsin deficiency (AATD, n = 23) were used for an exploratory proteomic study aimed at generating fingerprints of these groups that can be used in future pathophysiological and perhaps even clinical research. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was the platform applied for this hypothesis-free investigation. Analysis of pooled specimens resulted in the production of a "fingerprint" made of 44 proteins for NS/HS; 17 for COPD and 15 for the group of AATD subjects. Several inflammatory cytokines (IL-1α, IL-1β, IL-2; IL-12, α and β subunits, IL-15, interferon α and γ, tumor necrosis factor α); Type I and II cytokeratins; two SP-A isoforms; Calgranulin A and B and α1-antitrypsin were detected and validated through the use of surface enhanced laser-desorption ionization mass spectrometry (SELDI-MS) and/or by Western blot (WB) analysis. These results are the prelude of quantitative studies aimed at identifying which of these proteins hold promise as identifiers of differences that could distinguish healthy subjects from patients.

journal_name

Int J Mol Sci

journal_title

International journal of molecular sciences

authors

Fumagalli M,Ferrari F,Luisetti M,Stolk J,Hiemstra PS,Capuano D,Viglio S,Fregonese L,Cerveri I,Corana F,Tinelli C,Iadarola P

doi

10.3390/ijms131113894

subject

Has Abstract

pub_date

2012-10-29 00:00:00

pages

13894-910

issue

11

issn

1422-0067

pii

ijms131113894

journal_volume

13

pub_type

杂志文章

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