Abstract：
:Specific labelling of target cell surfaces using antibody-conjugated paramagnetic nanobeads is essential for efficient magnetic cell separation. However, studies examining parameters determining the kinetics of bead-cell binding are scarce. The present study determines the binding rates for specific and unspecific binding of 150 nm paramagnetic nanobeads to highly purified target and non-target cells. Beads bound to cells were enumerated spectrophotometrically. Results show that the initial bead-cell binding rate and saturation levels depend on initial bead concentration and fit curves of the form A(1 - exp(-kt)). Unspecific binding within conventional experimental time-spans (up to 60 min) was not detectable photometrically. For CD3-positive cells, the probability of specific binding was found to be around 80 times larger than that of unspecific binding.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Waseem S,Allen MA,Schreier S,Udomsangpetch R,Bhakdi SCdoi
10.3390/ijms15058821subject
Has Abstractpub_date
2014-05-19 00:00:00pages
8821-34issue
5issn
1422-0067pii
ijms15058821journal_volume
15pub_type
杂志文章abstract：:Innate lymphoid cells (ILC) are important players of early immune defenses in situations like lymphoid organogenesis or in case of immune response to inflammation, infection and cancer. Th1 and Th2 antagonism is crucial for the regulation of immune responses, however mechanisms are still unclear for ILC functions. ILC...
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