Abstract：
:Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases, metabolic syndrome and with mortality risk. Indeed, changes in blood levels of BDNF may reflect those of its tissue of origin and/or promote pathological dysfunctions. The measurement of BDNF amount in plasma or in serum has been characterized with particular attention in the impact of different anti-coagulants, clotting duration, temperature (≤21 °C) and delay in blood sample centrifugation as well as in stability of storage. However, the influences of normothermic conditions (37 °C) and of clotting duration on BDNF levels in human serum have not been investigated yet. Here, we showed that time and temperature during serum preparation could be taken into consideration to assess the association and/or impact of BDNF levels in the occurrence of pathological conditions.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Amadio P,Sandrini L,Ieraci A,Tremoli E,Barbieri SSdoi
10.3390/ijms18091987subject
Has Abstractpub_date
2017-09-15 00:00:00issue
9issn
1422-0067pii
ijms18091987journal_volume
18pub_type
杂志文章abstract：:Homologous recombination repair (HRR), non-homologous end-joining (NHEJ) and alternative NHEJ are major pathways that are utilized by cells for processing DNA double strand breaks (DNA-DSBs); their function plays an important role in the radiation resistance of tumor cells. Conflicting data exist regarding the role of...
journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-04-03 00:00:00
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更新日期：2014-11-14 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2008-01-01 00:00:00
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journal_title：International journal of molecular sciences
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doi：10.3390/ijms19092649
更新日期：2018-09-06 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-11-03 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2020-06-22 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-01-16 00:00:00
