Abstract:
:Insulin stimulates glucose uptake through the membrane translocation of GLUT4 and GLUT1. Peroxisome proliferator-activated receptor γ (PPARγ) enhances insulin sensitivity. Here, we demonstrate that insulin stimulates GLUT4 and GLUT1 translocation, and glucose uptake, by activating the signaling pathway involving nicotinic acid adenine dinucleotide phosphate (NAADP), a calcium mobilizer, in adipocytes. We also demonstrate that PPARγ mediates insulin sensitization by enhancing NAADP production through upregulation of CD38, the only enzyme identified for NAADP synthesis. Insulin produced NAADP by both CD38-dependent and -independent pathways, whereas PPARγ produced NAADP by CD38-dependent pathway. Blocking the NAADP signaling pathway abrogated both insulin-stimulated and PPARγ-induced GLUT4 and GLUT1 translocation, thereby inhibiting glucose uptake. CD38 knockout partially inhibited insulin-stimulated glucose uptake. However, CD38 knockout completely blocked PPARγ-induced glucose uptake in adipocytes and PPARγ-mediated amelioration of glucose tolerance in diabetic mice. These results demonstrated that the NAADP signaling pathway is a critical molecular target for PPARγ-mediated insulin sensitization.
journal_name
Cell Repjournal_title
Cell reportsauthors
Song EK,Lee YR,Kim YR,Yeom JH,Yoo CH,Kim HK,Park HM,Kang HS,Kim JS,Kim UH,Han MKdoi
10.1016/j.celrep.2012.10.018subject
Has Abstractpub_date
2012-12-27 00:00:00pages
1607-19issue
6issn
2211-1247pii
S2211-1247(12)00373-7journal_volume
2pub_type
杂志文章相关文献
Cell Reports文献大全abstract::Exposure to low-dose irradiation causes transiently elevated expression of the long ncRNA PARTICLE (gene PARTICLE, promoter of MAT2A-antisense radiation-induced circulating lncRNA). PARTICLE affords both a cytosolic scaffold for the tumor suppressor methionine adenosyltransferase (MAT2A) and a nuclear genetic platform...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.03.043
更新日期:2015-04-21 00:00:00
abstract::DNA wrapping around histone octamers generates nucleosomes, the basic compaction unit of eukaryotic chromatin. Nucleosome stability is carefully tuned to maintain DNA accessibility in transcription, replication, and repair. Using freely orbiting magnetic tweezers, which measure the twist and length of single DNA molec...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.12.022
更新日期:2015-01-13 00:00:00
abstract::Viral persistence specifically inhibits CD4 Th1 responses and promotes Tfh immunity, but the mechanisms that suppress Th1 cells and the disease consequences of their loss are unclear. Here, we demonstrate that the loss of CD4 Th1 cells specifically leads to progressive CD8 T cell decline and dysfunction during viral p...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.08.065
更新日期:2016-09-20 00:00:00
abstract::The root stem cell niche, which is composed of four mitotically inactive quiescent center (QC) cells and the surrounding actively divided stem cells in Arabidopsis, is critical for growth and root development. Here, we demonstrate that the Arabidopsis prohibitin protein PHB3 is required for the maintenance of root ste...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.12.105
更新日期:2018-01-30 00:00:00
abstract::Antibodies secreted within the intestinal tract provide protection from the invasion of microbes into the host tissues. Germinal center (GC) formation in lymph nodes and spleen strictly requires SLAM-associated protein (SAP)-mediated T cell functions; however, it is not known whether this mechanism plays a similar rol...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.01.032
更新日期:2020-02-11 00:00:00
abstract::Recent rapid progress in the field of mechanobiology has been driven by novel emerging tools and methodologies and growing interest from different scientific disciplines. Specific progress has been made toward understanding how cell mechanics is linked to intracellular signaling and the regulation of gene expression i...
journal_title:Cell reports
pub_type: 杂志文章,评审
doi:10.1016/j.celrep.2019.08.075
更新日期:2019-10-01 00:00:00
abstract::Desensitization, signaling, and trafficking of G-protein-coupled receptors (GPCRs) are critically regulated by multifunctional adaptor proteins, β-arrestins (βarrs). The two isoforms of βarrs (βarr1 and 2) share a high degree of sequence and structural similarity; still, however, they often mediate distinct functional...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.08.053
更新日期:2019-09-24 00:00:00
abstract::Natural killer (NK) cells possess potent cytotoxic mechanisms that need to be tightly controlled. Here, we explored the regulation and function of GPR56/ADGRG1, an adhesion G protein-coupled receptor implicated in developmental processes and expressed distinctively in mature NK cells. Expression of GPR56 was triggered...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.04.053
更新日期:2016-05-24 00:00:00
abstract::Amphotericin, miconazole, and ciclopirox are antifungal agents from three different drug classes that can effectively kill planktonic yeast, yet their complete fungicidal mechanisms are not fully understood. Here, we employ a systems biology approach to identify a common oxidative-damage cellular death pathway trigger...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2012.12.021
更新日期:2013-02-21 00:00:00
abstract::In Drosophila, it is thought that peptidoglycan recognition proteins (PGRPs) SA and LC structurally discriminate between bacterial peptidoglycans with lysine (Lys) or diaminopimelic (DAP) acid, respectively, thus inducing differential antimicrobial transcription response. Here, we find that accessibility to PG at the ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.04.103
更新日期:2019-05-21 00:00:00
abstract::Effector, but not naïve, T cells are activated by toll-like receptor-2 (TLR2) stimulation, leading to cytokine production and proliferation. We found that the differential response is attributable to the lack of expression of the adaptor protein TIRAP in naive T cells. TIRAP expression is induced upon T-cell receptor ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.107911
更新日期:2020-07-21 00:00:00
abstract::Pathogens trigger T cells to express distinct sets of effector proteins. To better understand the molecular mechanisms that drive functional specification, we used high-resolution mass spectrometry and label-free protein quantification to measure proteomic differences between the seven largest circulating human CD8+ T...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.04.014
更新日期:2017-05-02 00:00:00
abstract::One critical event in reprogramming to pluripotency is erasure of the somatic transcriptional program of starting cells. Here, we present the proof of principle of a strategy for reprogramming to pluripotency facilitated by small molecules that interfere with the somatic transcriptional memory. We show that mild chemi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2016.08.060
更新日期:2016-09-20 00:00:00
abstract::Accumulation of toxic amyloid oligomers is a key feature in the pathogenesis of amyloid-related diseases. Formation of mature amyloid fibrils is one defense mechanism to neutralize toxic prefibrillar oligomers. This mechanism is notably influenced by apolipoprotein E variants. Cells that produce mature amyloid fibrils...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.08.057
更新日期:2015-10-06 00:00:00
abstract::S1PR1 signaling has been shown to restrain the number and function of regulatory T (Treg) cells in the periphery under physiological conditions and in colitis models, but its role in regulating tumor-associated T cells is unknown. Here, we show that S1PR1 signaling in T cells drives Treg accumulation in tumors, limits...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.02.016
更新日期:2014-03-27 00:00:00
abstract::The large number of mutations identified across all cancers represents an untapped reservoir of targets that can be useful for therapeutic targeting if highly selective, mutation-specific reagents are available. We report here our attempt to generate such reagents: monoclonal antibodies against the most common R175H, ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.11.112
更新日期:2018-01-02 00:00:00
abstract::Diverse cell types can be reprogrammed into pluripotent stem cells by ectopic expression of Oct4 (Pou5f1), Klf4, Sox3, and Myc. Many of these induced pluripotent stem cells (iPSCs) retain memory, in terms of DNA methylation and histone modifications (epigenetic memory), of their cellular origins, and this may bias sub...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.01.075
更新日期:2018-03-06 00:00:00
abstract::Learning critically depends on the ability to rapidly form and store non-overlapping representations of the external world. In line with their postulated role in episodic memory, hippocampal place cells can undergo a rapid reorganization of their firing fields upon contextual manipulations. To explore the mechanisms u...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.03.031
更新日期:2018-04-03 00:00:00
abstract::Hox genes are required for the development of the intestinal cecum, a major organ of plant-eating species. We have analyzed the transcriptional regulation of Hoxd genes in cecal buds and show that they are controlled by a series of enhancers located in a gene desert flanking the HoxD cluster. The start site of two opp...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2013.09.002
更新日期:2013-10-17 00:00:00
abstract::Linking the molecular effects of mutations to fitness is central to understanding evolutionary dynamics. Here, we establish a quantitative relation between the global effect of mutations on the E. coli proteome and bacterial fitness. We created E. coli strains with specific destabilizing mutations in the chromosomal f...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.03.051
更新日期:2015-04-28 00:00:00
abstract::Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates RB and functions as a collaborative nuclear oncogene. The serine threonine kinase Akt plays a pivotal role in the control of cellular metabolism, survival, and mitogenic signaling. Herein, Akt1-mediated phosphorylation of downstream substrate...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108151
更新日期:2020-09-15 00:00:00
abstract::Vitamin D (VD) is a known differentiating agent, but the role of VD receptor (VDR) is still incompletely described in acute myeloid leukemia (AML), whose treatment is based mostly on antimitotic chemotherapy. Here, we present an unexpected role of VDR in normal hematopoiesis and in leukemogenesis. Limited VDR expressi...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.12.055
更新日期:2020-01-21 00:00:00
abstract::Over 80% of pancreatic ductal adenocarcinoma (PDA) patients are diagnosed with non-resectable late-stage disease that lacks effective neoadjuvant therapies. Stereotactic body radiation therapy (SBRT) has shown promise as an emerging neoadjuvant approach for treating PDA, and here, we report that its combination with l...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2019.08.095
更新日期:2019-10-08 00:00:00
abstract::Pancreatitis is an inflammatory disease of the exocrine pancreas and ranks among the most common gastrointestinal disorders. Inflamed tissues frequently experience conditions of insufficient oxygen availability, or hypoxia. Here, we demonstrate that hypoxia and consequent stabilization of the hypoxia-inducible factor ...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.05.071
更新日期:2018-06-19 00:00:00
abstract::The SWI/SNF chromatin remodeling complex is highly conserved from yeast to human, and aberrant SWI/SNF complexes contribute to human disease. The Snf5/SMARCB1/INI1 subunit of SWI/SNF is a tumor suppressor frequently lost in pediatric rhabdoid cancers. We examined the effects of Snf5 loss on the composition, nucleosome...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2017.02.017
更新日期:2017-02-28 00:00:00
abstract::We examined hematopoietic protein kinase 1 (HPK1), whose reliance on scaffold versus kinase functions for negative immune cell regulation is poorly understood and critical to its assessment as a viable drug target. We identify kinase-dependent roles for HPK1 in CD8 T cells that restrict their anti-viral and anti-tumor...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2018.09.012
更新日期:2018-10-02 00:00:00
abstract::Bone morphogenic proteins (BMPs) are members of the transforming growth factor β (TGF-β) cytokine family promoting differentiation, homeostasis, and self-renewal of multiple tissues. We show that signaling through the bone morphogenic protein receptor 1α (BMPR1α) sustains expression of FOXP3 in Treg cells in periphera...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108219
更新日期:2020-10-06 00:00:00
abstract::We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated, short-duration and long-duration spaceflight. Previous studies have identified miRNAs that regulate rodent responses to spaceflight in low-Earth orbit, and we have confirm...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2020.108448
更新日期:2020-12-08 00:00:00
abstract::Trimethylation at histone H3K27 is central to the polycomb repression system. Juxtaposed to H3K27 is a widely conserved phosphorylatable serine residue (H3S28) whose function is unclear. To assess the importance of H3S28, we generated a Drosophila H3 histone mutant with a serine-to-alanine mutation at position 28. H3S...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2015.04.055
更新日期:2015-06-09 00:00:00
abstract::Elevated levels of the free amino acid L-tryptophan (L-Trp) trigger expression of the tryptophanase tnaCAB operon in E. coli. Activation depends on tryptophan-dependent ribosomal stalling during translation of the upstream TnaC peptide. Here, we present a cryoelectron microscopy (cryo-EM) reconstruction at 3.8 Å resol...
journal_title:Cell reports
pub_type: 杂志文章
doi:10.1016/j.celrep.2014.09.011
更新日期:2014-10-23 00:00:00