Abstract：
:As antibody-based diagnosis and therapy grow at an increased pace, there is a need for methods which rapidly and accurately determine antibody-antigen interactions. Here, we report a method for the multiplex determination of antibody epitopes using bacterial cell-surface display. A protein-fragment library with 10(7) cell clones, covering 60 clinically-relevant protein targets, was created and characterized with massively parallel sequencing. Using this multi-target fragment library we determined simultaneously epitopes of commercial monoclonal and polyclonal antibodies targeting PSMA, EGFR, and VEGF. Off-target binding was observed for one of the antibodies, which demonstrates the methods ability to reveal cross-reactivity. We exemplify the detection of structural epitopes by mapping the therapeutic antibody Avastin. Based on our findings we suggest this method to be suitable for mapping linear and structural epitopes of monoclonal and polyclonal antibodies in a multiplex fashion and could find applicability in serum profiling as well as other protein-protein interaction studies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Hudson EP,Uhlen M,Rockberg Jdoi
10.1038/srep00706subject
Has Abstractpub_date
2012-01-01 00:00:00pages
706issn
2045-2322journal_volume
2pub_type
杂志文章abstract：:Polar bears are ice-associated marine mammals that are known to swim and dive, yet their aquatic behaviour is poorly documented. Reductions in Arctic sea ice are clearly a major threat to this species, but understanding polar bears' potential behavioural plasticity with respect to the ongoing changes requires knowledg...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-27947-4
更新日期：2018-06-26 00:00:00
abstract：:Liquid foams are classified into a dry foam and a wet foam, empirically judging from the liquid fraction or the shape of the gas bubbles. It is known that physical properties such as elasticity and diffusion are different between the dry foam and the wet foam. Nevertheless, definitions of those states have been vague ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37506
更新日期：2016-11-22 00:00:00
abstract：:Almost 150 years after the first identification of Neandertal skeletal material, the cognitive and symbolic abilities of these populations remain a subject of intense debate. We present 99 new Neandertal remains from the Troisième caverne of Goyet (Belgium) dated to 40,500-45,500 calBP. The remains were identified thr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29005
更新日期：2016-07-06 00:00:00
abstract：:Large bowel preparation may cause a substantial change in the gut microbiota and metabolites. Here, we included a bowel prep group and a no-procedure control group and evaluated the effects of bowel prep on the stability of the gut microbiome and metabolome as well as on recovery. Gut microbiota and metabolome composi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40182-9
更新日期：2019-03-11 00:00:00
abstract：:Molecular alignment underpins optical, mechanical, and thermal properties of materials, however, its direct measurement from volumes with micrometer dimensions is not accessible, especially, for structurally complex bio-materials. How the molecular alignment is linked to extraordinary properties of silk and its amorph...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07502-3
更新日期：2017-08-07 00:00:00
abstract：:The non-enzymatic browning, namely Maillard reaction is commonly invoked to account for abiotic chemical transformations of organic matter. Here we report a new reaction pathway via the Maillard reaction to systematically synthesize a series of nitrogen-doped carbon dots (C-dots) with superhigh quantum yield (QY) and ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03564
更新日期：2014-01-06 00:00:00
abstract：:Recently, slow earthquakes (slow EQ) have received much attention relative to understanding the mechanisms underlying large earthquakes and to detecting their precursors. Low-frequency earthquakes (LFE) are a specific type of slow EQ. In the present paper, we reveal the relevance of LFEs to the 11 March 2011 Great Toh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45765-0
更新日期：2019-06-27 00:00:00
abstract：:To develop a clinic-relevant protocol for systemic up-regulation of NFE2-related factor 2 (Nrf2) to prevent diabetic cardiomyopathy (DCM), male db/db and age-matched wild-type (WT) mice were given sulforaphane (SFN, an Nrf2 activator) and its natural source, broccoli sprout extract (BSE) by gavage every other day for ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30252
更新日期：2016-07-26 00:00:00
abstract：:Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complet...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25030
更新日期：2016-04-29 00:00:00
abstract：:Many drugs commonly prescribed during pregnancy lack a fetal safety recommendation - called FDA 'category C' drugs. This study aims to classify these drugs into harmful and safe categories using knowledge gained from chemoinformatics (i.e., pharmacological similarity with drugs of known fetal effect) and empirical dat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12943-x
更新日期：2017-10-09 00:00:00
abstract：:Abnormal expression of microRNAs (miRNAs) contributes to glioma initiation. However, the expression of miRNAs in tumour tissue or blood of spinal cord glioma (SCG) patients, particularly in high-grade spinal gliomas (Grade IV) known as glioblastoma (GBM), remains largely unknown. In this study we aimed to determine di...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-42364-x
更新日期：2019-06-24 00:00:00
abstract：:Using in vivo second harmonic generation (SHG) and third harmonic generation (THG) microscopies, we tracked the course of collagen remodeling over time in the same melanoma microenvironment within an individual mouse. The corresponding structural and morphological changes were quantitatively analyzed without labeling ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08879
更新日期：2015-03-09 00:00:00
abstract：:The extracellular matrix (ECM) impacts stem cell differentiation, but identifying formulations supportive of differentiation is challenging in 3D models. Prior efforts involving combinatorial ECM arrays seemed intuitively advantageous. We propose an alternative that suggests reducing sample size and technological burd...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18705
更新日期：2015-12-21 00:00:00
abstract：:Platelet Derived Growth Factor Receptor (PDGFR) signaling is a central mitogenic pathway in development, as well as tissue repair and homeostasis. The rules governing the binding of PDGF ligand to the receptor to produce activation and downstream signaling have been well defined over the last several decades. In cultu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75774-3
更新日期：2020-11-09 00:00:00
abstract：:The ability to extract the direction of the other's gaze allows us to shift our attention to an object of interest to the other and to establish joint attention. By mapping one's own intentions on the object of joint attention, humans develop a Theory of (the other's) Mind (TOM), a functional sequence possibly disrupt...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51783-9
更新日期：2019-10-25 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-69680-x
更新日期：2020-08-27 00:00:00
abstract：:Objectives were to evaluate the role of canonical WNT signaling in development of the preimplantation embryo. Signaling was activated with 2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine (AMBMP) and inhibited with Dickkopf-related protein 1 (DKK1). Treatment of bovine embryos with AMBMP at da...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01266
更新日期：2013-01-01 00:00:00
abstract：:Saturn rings are most beautiful and dynamic places in the solar system, consisting of ice particles in a constant battle between the gravitational forces of Saturn and its many moons. Fan, spiral, propellers, moonlets and streamer-channels observed by CASSINI in the F-ring have been attributed to encounters by Prometh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01276
更新日期：2013-01-01 00:00:00
abstract：:Sex-based variations in the immune response to the influenza vaccines was reported, however, the genetic basis responsible for the sex variations in the immune response toward the influenza vaccines remains unclear. Here, the genes responsible for sex-specific responses after vaccination with trivalent inactivated inf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31999-x
更新日期：2018-09-10 00:00:00
abstract：:The physiological importance of CD151 tetraspanin is known from somatic cells and its outside-in signalling through integrins was described. In male germ cells, two tetraspanins, CD9 and CD81, are involved in sperm-egg membrane fusion, and similarly to integrins, they occupy characteristic regions. We report here on a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61334-2
更新日期：2020-03-09 00:00:00
abstract：:The inactivation of pathogens in liquids has broad applications, ranging from water disinfection to food pasteurization. However, common cell inactivation methods (e.g., chlorination, ultraviolet radiation and thermal treatment) have significant drawbacks such as carcinogenic byproduct formation, energy intensiveness ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-34027-0
更新日期：2018-10-25 00:00:00
abstract：:This study aims to identify a panel of blood-cell neuroplasticity-related genes expressed following environmental enrichment stimulation (EE). The Drug detection (DD) training course was an excellent model for the study of EE in the working dog. This research is divided into two experimental trials. In the First Trial...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43402-4
更新日期：2019-05-06 00:00:00
abstract：:Cardiac resynchronization therapy (CRT) threw lights on heart failure treatment, however, parts of patients showed nonresponse to CRT. Unfortunately, it lacks effective parameters to predict CRT non-response. In present study, we try to seek effective electro-echocardiographic predictors on CRT non-response. This is a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44009
更新日期：2017-03-10 00:00:00
abstract：:Angelica gigas Nakai (AGN) is an oriental traditional medicine to treat anemia, dysmenorrhea, and migraine. However, its anti-lymphoma effect is yet to be tested. Here, we demonstrated that AGN and its major component decursin target Myc to suppress lymphomagenesis in vitro and in vivo. AGN inhibited cell viability in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28619-z
更新日期：2018-07-12 00:00:00
abstract：:In this study, nanowires/nanowalls were generated on a silicon wafer through a chemical etching method. Octadecyltrichlorosilane (OTS) was deposited onto the nanowire/nanowall surfaces to alter their hydrophobicity. The hydrophobic characteristics of the surfaces were further modified via a 1.5-μm-thick layer of n-oct...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38678
更新日期：2016-12-09 00:00:00
abstract：:Grazing and burrowing organisms usually homogenise microalgal mats that form on benthic sediments of many aquatic ecosystems. In the absence of this disruption, microalgal mats can accrete laminated deposits (stromatolites). Stromatolites are rare in modern coastal ecosystems, but persist at locations where metazoans ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42614
更新日期：2017-02-16 00:00:00
abstract：:Food and flavour pairing are commonly used as an empirically based phenomenology by chefs and food innovators for creating delicious dishes. However, there is little if any science behind the pairing systems used, and it appears that pairing is determined by food culture and tradition rather than by chemical food comp...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77107-w
更新日期：2020-11-18 00:00:00
abstract：:Foaming negatively affects anaerobic digestion of food waste (FW). To identify the causes of foaming, reactor performance and microbial community dynamics were investigated in three mesophilic digesters treating FW. The digesters were operated under different modes, and foaming was induced with several methods. Prolif...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14258-3
更新日期：2017-10-20 00:00:00
abstract：:SLC30A2 encodes a zinc (Zn) transporter (ZnT2) that imports Zn into vesicles in highly-specialized secretory cells. Numerous mutations and non-synonymous variants in ZnT2 have been reported in humans and in breastfeeding women; ZnT2 variants are associated with abnormally low milk Zn levels and can lead to severe infa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21505-8
更新日期：2018-02-23 00:00:00
abstract：:SRY, a sex-determining gene, induces testis development in chromosomally female (XX) individuals. However, mouse XX Sertoli cells carrying Sry (XX/Sry Sertoli cells) are incapable of fully supporting germ cell development, even when the karyotype of the germ cells is XY. While it has therefore been assumed that XX/Sry...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep41912
更新日期：2017-02-02 00:00:00
