An integrated statistical model for enhanced murine cardiomyocyte differentiation via optimized engagement of 3D extracellular matrices.

Abstract:

:The extracellular matrix (ECM) impacts stem cell differentiation, but identifying formulations supportive of differentiation is challenging in 3D models. Prior efforts involving combinatorial ECM arrays seemed intuitively advantageous. We propose an alternative that suggests reducing sample size and technological burden can be beneficial and accessible when coupled to design of experiments approaches. We predict optimized ECM formulations could augment differentiation of cardiomyocytes derived in vitro. We employed native chemical ligation to polymerize 3D poly (ethylene glycol) hydrogels under mild conditions while entrapping various combinations of ECM and murine induced pluripotent stem cells. Systematic optimization for cardiomyocyte differentiation yielded a predicted solution of 61%, 24%, and 15% of collagen type I, laminin-111, and fibronectin, respectively. This solution was confirmed by increased numbers of cardiac troponin T, α-myosin heavy chain and α-sarcomeric actinin-expressing cells relative to suboptimum solutions. Cardiomyocytes of composites exhibited connexin43 expression, appropriate contractile kinetics and intracellular calcium handling. Further, adding a modulator of adhesion, thrombospondin-1, abrogated cardiomyocyte differentiation. Thus, the integrated biomaterial platform statistically identified an ECM formulation best supportive of cardiomyocyte differentiation. In future, this formulation could be coupled with biochemical stimulation to improve functional maturation of cardiomyocytes derived in vitro or transplanted in vivo.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Jung JP,Hu D,Domian IJ,Ogle BM

doi

10.1038/srep18705

subject

Has Abstract

pub_date

2015-12-21 00:00:00

pages

18705

issn

2045-2322

pii

srep18705

journal_volume

5

pub_type

杂志文章
  • Acfs: accurate circRNA identification and quantification from RNA-Seq data.

    abstract::Circular RNAs (circRNAs) are a group of single-stranded RNAs in closed circular form. They are splicing-generated, widely expressed in various tissues and have functional implications in development and diseases. To facilitate genome-wide characterization of circRNAs using RNA-Seq data, we present a freely available s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38820

    authors: You X,Conrad TO

    更新日期:2016-12-08 00:00:00

  • A germline mutation in SRRM2, a splicing factor gene, is implicated in papillary thyroid carcinoma predisposition.

    abstract::Papillary thyroid carcinoma (PTC) displays strong but so far largely uncharacterized heritability. Here we studied genetic predisposition in a family with six affected individuals. We genotyped all available family members and conducted whole exome sequencing of blood DNA from two affected individuals. Haplotype analy...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10566

    authors: Tomsic J,He H,Akagi K,Liyanarachchi S,Pan Q,Bertani B,Nagy R,Symer DE,Blencowe BJ,de la Chapelle A

    更新日期:2015-07-02 00:00:00

  • Inactivation of HMGCL promotes proliferation and metastasis of nasopharyngeal carcinoma by suppressing oxidative stress.

    abstract::Altered metabolism is considered as a hallmark of cancer. Here we investigated expression of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) 2 lyase (HMGCL), an essential enzyme in ketogenesis, which produces ketone bodies by the breakdown of fatty acids to supply energy, in nasopharyngeal carcinoma (NPC). The express...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11025-2

    authors: Luo W,Qin L,Li B,Liao Z,Liang J,Xiao X,Xiao X,Mo Y,Huang G,Zhang Z,Zhou X,Li P

    更新日期:2017-09-20 00:00:00

  • Diversity of key players in the microbial ecosystems of the human body.

    abstract::Coexisting bacteria form various microbial communities in human body parts. In these ecosystems they interact in various ways and the properties of the interaction network can be related to the stability and functional diversity of the local bacterial community. In this study, we analyze the interaction network among ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep15920

    authors: Jordán F,Lauria M,Scotti M,Nguyen TP,Praveen P,Morine M,Priami C

    更新日期:2015-10-30 00:00:00

  • Gene expression profiles for low-dose exposure to diethyl phthalate in rodents and humans: a translational study with implications for breast carcinogenesis.

    abstract::Phthalates are commonly included as ingredients in personal care products such as cosmetics, shampoos and perfumes. Diethyl phthalate (DEP) has been found to be anti-androgenic and linked with adverse reproductive effects on males, but effects on females are poorly understood. We designed an integrative and translatio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-63904-w

    authors: Gopalakrishnan K,Aushev VN,Manservisi F,Falcioni L,Panzacchi S,Belpoggi F,Parada H Jr,Garbowski G,Hibshoosh H,Santella RM,Gammon MD,Teitelbaum SL,Chen J

    更新日期:2020-04-27 00:00:00

  • Massive gene loss in mistletoe (Viscum, Viscaceae) mitochondria.

    abstract::Parasitism is a successful survival strategy across all kingdoms and has evolved repeatedly in angiosperms. Parasitic plants obtain nutrients from other plants and some are agricultural pests. Obligate parasites, which cannot complete their lifecycle without a host, may lack functional photosystems (holoparasites), or...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17588

    authors: Petersen G,Cuenca A,Møller IM,Seberg O

    更新日期:2015-12-02 00:00:00

  • New Bicyclic Cembranoids from the South China Sea Soft Coral Sarcophyton trocheliophorum.

    abstract::Nine new bicyclic cembranoids, sarcophytrols M-U(1-9), were isolated from the South China Sea soft coral Sarcophyton trocheliophorum as minor components, along with one known related cembranoid 10. Their structures were elucidated by detailed spectroscopic analysis and chemical conversion. The chemical structures of t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep46584

    authors: Liang LF,Chen WT,Li XW,Wang HY,Guo YW

    更新日期:2017-04-21 00:00:00

  • Highly hydrophilic poly(vinylidene fluoride)/meso-titania hybrid mesoporous membrane for photocatalytic membrane reactor in water.

    abstract::The high hydrophobicity of poly(vinylidene fluoride) (PVDF) membrane remains an obstacle to be applied in some purification processes of water or wastewater. Herein, a highly hydrophilic hybrid mesoporous titania membrane composed of mesoporous anatase titania (meso-TiO2) materials inside the three-dimensional (3D) ma...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19148

    authors: Wang M,Yang G,Jin P,Tang H,Wang H,Chen Y

    更新日期:2016-01-12 00:00:00

  • Four-dimensional in vivo X-ray microscopy with projection-guided gating.

    abstract::Visualizing fast micrometer scale internal movements of small animals is a key challenge for functional anatomy, physiology and biomechanics. We combine phase contrast tomographic microscopy (down to 3.3 μm voxel size) with retrospective, projection-based gating (in the order of hundreds of microseconds) to improve th...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08727

    authors: Mokso R,Schwyn DA,Walker SM,Doube M,Wicklein M,Müller T,Stampanoni M,Taylor GK,Krapp HG

    更新日期:2015-03-12 00:00:00

  • Pleasantness and trigeminal sensations as salient dimensions in organizing the semantic and physiological spaces of odors.

    abstract::A major issue in human olfaction research is to characterize the main dimensions that organize the space of odors. The present study examines this question and shows that, beside pleasantness, trigeminal sensations, and particularly irritation, play an important role. These results were consistent along two different ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-26510-5

    authors: Licon CC,Manesse C,Dantec M,Fournel A,Bensafi M

    更新日期:2018-05-31 00:00:00

  • Applications and limitations of fitting of the operational model to determine relative efficacies of agonists.

    abstract::Proper determination of agonist efficacy is essential in the assessment of agonist selectivity and signalling bias. Agonist efficacy is a relative term that is dependent on the system in which it is measured, especially being dependent on receptor expression level. The operational model (OM) of functional receptor ago...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40993-w

    authors: Jakubík J,Randáková A,Rudajev V,Zimčík P,El-Fakahany EE,Doležal V

    更新日期:2019-03-15 00:00:00

  • Multiple climate cooling prior to Sturtian glaciations: evidence from chemical index of alteration of sediments in South China.

    abstract::Investigation of climatic conditions prior to the Sturtian glaciations is critical to understanding the trigger mechanism for the series of Neoproterozoic global glaciations. In this study, we report high-resolution chemical index of alteration (CIA) records in the sediments of South China prior to the Sturtian glacia...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep06868

    authors: Huang J,Feng L,Lu D,Zhang Q,Sun T,Chu X

    更新日期:2014-10-31 00:00:00

  • Molecular-trapping in Emulsion's Monolayer: A New Strategy for Production and Purification of Bioactive Saponins.

    abstract::Saponins from defatted root-extract of Securidaca longipedunculata were systematically entrapped in emulsion monolayer-barrier and finally recovered in pure form through demulsification. First, their molecules were dispersed in water to engineer a monomolecular film architecture, via self-assembly. Emulsifying with et...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15067-4

    authors: Obasi TC,Moldovan R,Toiu A,Braicu C,Bodoki E,Berindan-Neagoe I,Oniga I,Sandulescu R,Oprean R

    更新日期:2017-11-06 00:00:00

  • The Brain Metabolome of Male Rats across the Lifespan.

    abstract::Comprehensive and accurate characterization of brain metabolome is fundamental to brain science, but has been hindered by technical limitations. We profiled the brain metabolome in male Wistar rats at different ages (day 1 to week 111) using high-sensitivity and high-resolution mass spectrometry. Totally 380 metabolit...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24125

    authors: Zheng X,Chen T,Zhao A,Wang X,Xie G,Huang F,Liu J,Zhao Q,Wang S,Wang C,Zhou M,Panee J,He Z,Jia W

    更新日期:2016-04-11 00:00:00

  • Spatial accessibility of general inpatient care in Germany: an analysis of surgery, internal medicine and neurology.

    abstract::Improving spatial accessibility to hospitals is a major task for health care systems which can be facilitated using recent methodological improvements of spatial accessibility measures. We used the integrated floating catchment area (iFCA) method to analyze spatial accessibility of general inpatient care (internal med...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-76212-0

    authors: Bauer J,Klingelhöfer D,Maier W,Schwettmann L,Groneberg DA

    更新日期:2020-11-05 00:00:00

  • Formation of Magnetic Anisotropy by Lithography.

    abstract::Artificial interface anisotropy is demonstrated in alternating Co/Pt and Co/Pd stripe patterns, providing a means of forming magnetic anisotropy using lithography. In-plane hysteresis loops measured along two principal directions are explained in depth by two competing shape and interface anisotropies, thus confirming...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26709

    authors: Kim SN,Nam YJ,Kim YD,Choi JW,Lee H,Lim SH

    更新日期:2016-05-24 00:00:00

  • Investigating the Life Expectancy and Proteolytic Degradation of Engineered Skeletal Muscle Biological Machines.

    abstract::A combination of techniques from 3D printing, tissue engineering and biomaterials has yielded a new class of engineered biological robots that could be reliably controlled via applied signals. These machines are powered by a muscle strip composed of differentiated skeletal myofibers in a matrix of natural proteins, in...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03723-8

    authors: Cvetkovic C,Ferrall-Fairbanks MC,Ko E,Grant L,Kong H,Platt MO,Bashir R

    更新日期:2017-06-19 00:00:00

  • Polyester type polyHIPE scaffolds with an interconnected porous structure for cartilage regeneration.

    abstract::Development of artificial materials for the facilitation of cartilage regeneration remains an important challenge in orthopedic practice. Our study investigates the potential for neocartilage formation within a synthetic polyester scaffold based on the polymerization of high internal phase emulsions. The fabrication o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28695

    authors: Naranda J,Sušec M,Maver U,Gradišnik L,Gorenjak M,Vukasović A,Ivković A,Rupnik MS,Vogrin M,Krajnc P

    更新日期:2016-06-24 00:00:00

  • Bi-functional IgG-lysosomal enzyme fusion proteins for brain drug delivery.

    abstract::Most lysosomal storage disorders affect the central nervous system. However, lysosomal enzymes do not cross the blood-brain barrier (BBB), and intravenous enzyme infusion is not effective for the brain. Lysosomal enzymes can be re-engineered for BBB transport as IgG-enzyme fusion proteins, where the IgG domain is a mo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-55136-4

    authors: Boado RJ,Lu JZ,Hui EK,Lin H,Pardridge WM

    更新日期:2019-12-09 00:00:00

  • Campylobacter gastroenteritis in children in north-eastern Israel comparison with other common pathogens.

    abstract::Gastroenteritis is common among children. Campylobacter jejuni is one of the main causative bacterial pathogens, together with Shigella, Salmonella and invasive Escherichia coli. Campylobacteriosis is a zoonotic, usually self-limited disease that does not always require antibiotic treatment. In cases of protracted dia...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62744-y

    authors: Sakran W,Hexner-Erlichman Z,Spiegel R,Batheesh H,Halevy R,Koren A

    更新日期:2020-04-02 00:00:00

  • microRNA-352 regulates collateral vessel growth induced by elevated fluid shear stress in the rat hind limb.

    abstract::Although collateral vessel growth is distinctly enhanced by elevated fluid shear stress (FSS), the underlying regulatory mechanism of this process remains incompletely understood. Recent studies have shown that microRNAs (miRNAs) play a pivotal role in vascular development, homeostasis and a variety of diseases. There...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-06910-9

    authors: Guan Y,Cai B,Wu X,Peng S,Gan L,Huang D,Liu G,Dong L,Xiao L,Liu J,Zhang B,Cai WJ,Schaper J,Schaper W

    更新日期:2017-07-27 00:00:00

  • Cationic bactericidal peptide 1018 does not specifically target the stringent response alarmone (p)ppGpp.

    abstract::The bacterial stringent response is a key regulator of bacterial virulence, biofilm formation and antibiotic tolerance, and is a promising target for the development of new antibacterial compounds. The intracellular nucleotide (p)ppGpp acts as a messenger orchestrating the stringent response. A synthetic peptide 1018 ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep36549

    authors: Andresen L,Tenson T,Hauryliuk V

    更新日期:2016-11-07 00:00:00

  • Oxytocin administration suppresses hypothalamic activation in response to visual food cues.

    abstract::The aim of this study was to use functional neuroimaging to investigate whether oxytocin modulates the neural response to visual food cues in brain regions involved in the control of food intake. Twenty-four normal weight volunteers received intranasal oxytocin (24 IU) or placebo in a double-blind, randomized crossove...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-04600-0

    authors: van der Klaauw AA,Ziauddeen H,Keogh JM,Henning E,Dachi S,Fletcher PC,Farooqi IS

    更新日期:2017-06-27 00:00:00

  • Machine learning as an improved estimator for magnetization curve and spin gap.

    abstract::The magnetization process is a very important probe to study magnetic materials, particularly in search of spin-liquid states in quantum spin systems. Regrettably, however, progress of the theoretical analysis has been unsatisfactory, mostly because it is hard to obtain sufficient numerical data to support the theory....

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70389-0

    authors: Nakamura T

    更新日期:2020-08-26 00:00:00

  • HARDI-ZOOMit protocol improves specificity to microstructural changes in presymptomatic myelopathy.

    abstract::Diffusion magnetic resonance imaging (dMRI) proved promising in patients with non-myelopathic degenerative cervical cord compression (NMDCCC), i.e., without clinically manifested myelopathy. Aim of the study is to present a fast multi-shell HARDI-ZOOMit dMRI protocol and validate its usability to detect microstructura...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70297-3

    authors: Labounek R,Valošek J,Horák T,Svátková A,Bednařík P,Vojtíšek L,Horáková M,Nestrašil I,Lenglet C,Cohen-Adad J,Bednařík J,Hluštík P

    更新日期:2020-10-16 00:00:00

  • Experimental study on mandibular length and facial symmetry of low estrogen level and anterior disc displacement of temporomandibular joint.

    abstract::This study was aimed at elucidating the changes of mandible symmetry in a rabbit model with low estrogen levels induced by ovariectomy (OVX) combined with temporomandibular joint (TMJ) anterior disc displacement without reduction (ADDWoR). 32 growing rabbits were randomly allocated into 4 groups; OVX group, ADDWoR gro...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-34023-4

    authors: Jiao Z,Wang X,Zhang X,Yang C

    更新日期:2018-10-23 00:00:00

  • Fine-tuning the expression of target genes using a DDI2 promoter gene switch in budding yeast.

    abstract::Tuned gene expression is crucial to the proper growth and response to the environmental changes of an organism. To enable tunable gene expression as designed is desirable in both scientific research and industrial application. Here, we introduce a novel promoter switching method based on the DDI2 promoter (PDDI2) that...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49000-8

    authors: Wang Y,Zhang K,Li H,Xu X,Xue H,Wang P,Fu YV

    更新日期:2019-08-29 00:00:00

  • Retraction Note: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells.

    abstract::This paper has been retracted at the request of the authors. ...

    journal_title:Scientific reports

    pub_type: 撤回出版物

    doi:10.1038/s41598-018-31224-9

    authors: Wang W,Jia YL,Li YC,Jing CQ,Guo X,Shang XF,Zhao CP,Wang TY

    更新日期:2018-09-04 00:00:00

  • Association between Hepatitis B Virus Infection and Metabolic Syndrome in Southwest China: A Cross-sectional Study.

    abstract::The correlation between hepatitis B virus (HBV) infection and metabolic syndrome (MetS) remains to be clarified. In this study, we explored this association in a large population in Southwest China. This was a cross-sectional study, with pooled adult health data. Multivariate logistic regression analysis, controlling ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-62609-4

    authors: Yan LB,Liao J,Han N,Zhou LY,Wang XE,Wang YJ,Tang H

    更新日期:2020-04-21 00:00:00

  • Value of corneal epithelial and Bowman's layer vertical thickness profiles generated by UHR-OCT for sub-clinical keratoconus diagnosis.

    abstract::Ultra-high resolution optical coherence tomography (UHR-OCT) can image the corneal epithelium and Bowman's layer and measurement the thicknesses. The purpose of this study was to validate the diagnostic power of vertical thickness profiles of the corneal epithelium and Bowman's layer imaged by UHR-OCT in the diagnosis...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/srep31550

    authors: Xu Z,Jiang J,Yang C,Huang S,Peng M,Li W,Cui L,Wang J,Lu F,Shen M

    更新日期:2016-08-11 00:00:00