Abstract:
:We examined specific expression of protein kinase C (PK-C) isozymes in cultured human glial and neuronal cell lines, using type-specific monoclonal antibodies MC-1a, -2a, and -3a (Hidaka H. et al., J. Biol. Chem., 263 (1988) 4523-4526). Immunoblotting experiments revealed that a 80 kDa band of three kinds of glioblastoma cells (A-172, SK-MG-1, SK-MG-4) was stained with MC-3a, whereas that of neuroblastoma cells (SK-N-MC) reacted with MC-2a. Immunoenzymetric assay showed that glioblastoma cells (A-172, SK-MG-1, SK-MG-4) contained 127.6 +/- 14.4, 248.8 +/- and 148.5 +/- 35.8 ng/mg protein of type III. respectively, while neuroblastoma cells (SK-N-MC) contained 389.5 +/- 20.7 ng/mg protein of type II. These results suggest that PK-C isozymes may be specifically expressed, depending on types of central nervous system (CNS) tumor cells.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Shimosawa S,Hachiya T,Hagiwara M,Usuda N,Sugita K,Hidaka Hdoi
10.1016/0304-3940(90)90698-9subject
Has Abstractpub_date
1990-01-01 00:00:00pages
11-6issue
1-2eissn
0304-3940issn
1872-7972pii
0304-3940(90)90698-9journal_volume
108pub_type
杂志文章abstract::Wild-type neuronal calcium-binding protein VILIP (visinin-like protein), and a myristoylation mutant of VILIP which lacks the consensus sequence for N-terminal myristoylation, have been stably transfected in PC12 cells. Immunocytochemical studies of VILIP-transfected PC12 cells have revealed the wild-type VILIP is str...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00201-2
更新日期:1997-04-04 00:00:00
abstract::A broad spectrum caspase inhibitor reduces hypoxic ischemic brain injury. We hypothesized that a specific caspase-9 inhibitor would provide similar protection. Seven-day-old rat pups had the right carotid artery ligated, then were subjected to 2.5 h of 8% oxygen. Caspase-9 activity in the right cortex was measured enz...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00466-x
更新日期:2003-07-03 00:00:00
abstract::Inattention and impulsivity are the two most important indices for evaluations of ADHD. Currently, inattention and impulsivity were evaluated by clinical scales. The intelligent evaluation of the two indices using machine learning remains largely unexplored. This paper aimed to build regression modes for inattention a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2020.134874
更新日期:2020-04-17 00:00:00
abstract::The effect of acute phenylethanolamine-N-methyltransferase (PNMT) inhibition on rat brain catecholamine concentrations was studied. All PNMT inhibitors tested were effective in depleting hypothalamic and brain stem epinephrine. Norepinephrine concentrations were unaffected; however, dopamine levels were increased in a...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(80)90235-9
更新日期:1980-10-20 00:00:00
abstract::In experiments using a synaptosomal preparation from the striatum and nucleus accumbens, somatostatin caused a dose-dependent increase in dopamine synthesis. This increase was additive with that produced by 8-BrcAMP but not with that produced by the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA), and was bl...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90129-7
更新日期:1987-11-23 00:00:00
abstract::Motoneuron death induced by sciatic nerve transection in neonatal rats has been related to induction of the neuronal isoform of nitric oxide synthase (nNOS), a diaphorase of which one of the cofactors is nicotinamide adenine dinucleotide phosphate (NADPH). We transected the sciatic nerve of neonatal rats (P2) and exam...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01931-0
更新日期:2001-07-13 00:00:00
abstract::Effects of intrathecally (i.t.) administered R-phenylisopropyl adenosine (R-PIA), an adenosine A1 receptor agonist, on presumed pain behaviour were assessed in a rat model of chronic central pain. Spinal cord injury was induced photochemically via laser irradiation of the spinal cord after intravenous injection of ery...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00092-5
更新日期:1998-02-27 00:00:00
abstract:OBJECTIVE:The study aimed to explore the modification to cortical oscillations of Parkinson disease (PD) patients by subthalamic nucleus deep brain stimulation (STN DBS). METHODS:With Magnetoencephalogram (MEG) detection, we examined the changes in absolute power spectrum of cortical oscillations in the PD patients wi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.11.009
更新日期:2017-01-01 00:00:00
abstract::An antiserum to choline acetyltransferase, partially purified from bovine brain (anti-ChAT 1), when incubated with synaptosomes prepared from rat cerebral cortex in the presence of complement caused release of 10% of lactate dehydrogenase and 27% of ChAT. Sodium-dependent uptake of choline was totally abolished. Simil...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(86)90469-6
更新日期:1986-10-08 00:00:00
abstract::The transcriptional co-activator p300 plays an important role in regulating gene expression in a number of different cell types. We have shown that wild type (WT) Presenilin 1 (PS1) stimulates the transcriptional activity ability of CREB Binding Protein (CBP), a close homolog of p300, whereas the Alzheimer's disease (...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.11.036
更新日期:2007-02-14 00:00:00
abstract::Ablation of the frog's nucleus isthmi results in a visual scotoma contralateral to the lesion. Within the scotoma, animals do not respond to visually presented prey or threats. The locus of visual loss is related to the area of isthmal tissue ablated. With complete unilateral ablation, a frog displays no visually elic...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(85)80096-3
更新日期:1985-03-15 00:00:00
abstract::A neurogenic contribution to joint inflammation has been demonstrated in rat adjuvant arthritis, however as inflammatory mechanisms vary between species it is unclear whether these observations can be applied more generally. The aim of this study was to assess the neurogenic contribution to cellular infiltration and o...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01790-0
更新日期:2001-02-23 00:00:00
abstract::A novel preparation for the study of male and female sexual function in anesthetized, acutely spinalized rats is reported. In both sexes, the coitus reflex (the neuromuscular concomitants of sexual climax) could be elicited by mechanical stimulation of the distal urethra. It is concluded that the spinal sexual circuit...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(88)90042-0
更新日期:1988-12-05 00:00:00
abstract::Positron emission tomography (PET) during focal repetitive transcranial magnetic stimulation (rTMS) has emerged as a promising approach to study cortical connectivity in awake humans. However, the noise caused by the discharging magnetic coil might have confounding effects on the rTMS-related cortical activation patte...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(99)00454-1
更新日期:1999-07-23 00:00:00
abstract::Nerve growth factor (NGF) was injected in the brain of neonatal rats. In eleven of thirty-one rats that received 1 microgram of mouse NGF (2.5S form) on postnatal day 3, NGF receptor-like immunoreactivity was found in the preoptic area (POA) and hypothalamus until 12 days after birth. However, no immunoreactivity was ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(92)90924-v
更新日期:1992-04-27 00:00:00
abstract::Tinnitus is the perception of sound in the absence of any external auditory stimulus. Based on previous research we have proposed a framework which postulates that the reduction of ongoing inhibitory alpha activity in tinnitus subjects favors a synchronization of neurons in the gamma frequency range while in a resting...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2009.02.028
更新日期:2009-04-10 00:00:00
abstract::Evidence indicates that adaptive responses to systemic stress occur in retina, as indicated by the changes in sympathetic and sensory afferent neurotransmitters in the retina when under stressful stimulation, such as acute myocardial infarction. The aim of this study was to investigate the potential association of the...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.10.006
更新日期:2011-01-03 00:00:00
abstract::The present study tests the hypothesis that nitration is a potential mechanism of N-methyl-D-aspartate (NMDA) receptor modification, by assessing the effect of peroxynitrite in vitro on the glutamate and ion-channel sites of the NMDA receptor in the fetal guinea pig. Nitration of NMDA receptor subunits was confirmed b...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(00)01608-6
更新日期:2000-12-15 00:00:00
abstract::Non-photic stimuli, such as diurnal wheel running in rodents, phase shift the circadian clock and suppress the expression of Per1 in the suprachiasmatic nucleus (SCN). The goal of the present study was to directly decrease Per1 expression using antisense (AS) oligodeoxynucleotides to determine if such suppression prod...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.02.061
更新日期:2004-05-20 00:00:00
abstract::Immunohistochemical and immunoblot techniques were employed to examine the distribution and expression of GABA(B) receptors in the prefrontal cortex of postmortem subjects with schizophrenia and bipolar disorder. GABA(B)R1a/b immunoreactivity was observed in the neuronal soma and dendrites as well as in the neuropil i...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.04.025
更新日期:2005-08-05 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is produced both in the central nervous system (CNS) and the periphery. Effective in ameliorating neurodegeneration in several animal models of CNS disease, its promise as a therapeutic agent would be greatly enhanced if it readily crossed the blood-brain barrier (BBB...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00007-7
更新日期:2003-04-17 00:00:00
abstract::Parkinson's disease (PD) is characterized by bradykinesia, resting tremor, rigidity and postural instability as well as early symptoms. Previous studies that evaluated the association between H1/H2 MAPT haplotype and PD were mostly conducted in European populations in which the H1 haplotype was a reported risk factor ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2018.10.029
更新日期:2019-01-18 00:00:00
abstract::Sciatic axotomy in 2-day-old rats (P2) causes lumbar motoneuron loss, which could be associated with nitric oxide (NO) production. NO may be produced by three isoforms of synthase (NOS): neuronal (nNOS), endothelial (eNOS) and inducible (iNOS). We investigated NOS expression and NO synthesis in the lumbar enlargement ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.08.035
更新日期:2006-10-23 00:00:00
abstract::To test the hypothesis that the distribution of the pathology in variant Creutzfeldt-Jakob disease (vCJD) represents haematogenous spread of the disease, we studied the spatial correlation between the vacuolation, prion protein (PrP) deposits, and the blood vessel profiles in the cerebral cortex, hippocampus, dentate ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(03)00696-7
更新日期:2003-09-04 00:00:00
abstract::Rats with their intra-lingual taste nerves (the chorda tympani and the glossopharyngeal nerve) sectioned were trained to avoid drinking 0.5 M NaCl solution by associating NaCl drinking with subsequent LiCl poisoning. The acquired learned aversion in these rats was abolished after ablations of the neocortical area for ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(81)90235-4
更新日期:1981-07-02 00:00:00
abstract::Vesicular monoamine transporter 2 (VMAT2) is responsible for packing dopamine into vesicles, and reduces the effects of neurotoxins by sequestering them into vesicles. In this report, we tested the hypothesis that VMAT2 is associated with Lewy body (LB) formation by immunohistochemical staining of midbrain and cortica...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.11.068
更新日期:2006-04-03 00:00:00
abstract::The study investigated the effects of metabolic syndrome on urinary bladder nerve-growth factor (NGF) and p75(NTR) expression in fructose-fed obese rats. Age-matched male Wistar rats were divided into four groups; group I: normal control rats; group II: 6-week fructose-fed rats (FR); group III: 9-week FR; and group IV...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.06.008
更新日期:2008-08-15 00:00:00
abstract::myo-Inositol monophosphatase (E.C.3.1.3.25) catalyzes the hydrolysis of myo-inositol 1-phosphate in the presence of Mg2+ at a physiologic pH to form free myo-inositol, maintaining a supply that represents the precursor for inositol phospholipid second messenger signaling systems. In the present study the activity and ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(98)00209-2
更新日期:1998-04-10 00:00:00
abstract::In the rat, electrical stimulation of the cranial end of the cut hepatic branch of the vagus nerve modulated gastric contractions. When the gastric contraction rate reached approximately one per min, nerve stimulation caused total suppression. When the stomach was free of contraction, nerve stimulation initiated one. ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(85)90097-7
更新日期:1985-01-07 00:00:00
abstract::We recently showed that dopamine (DA) and serotonin (5-HT) exert anticonvulsant effects against limbic seizures in rats mediated by hippocampal D(2) and 5-HT(1A) receptor stimulation. For exogenously administered monoamines, anticonvulsant activity was only observed following 70--400% and 80--350% increases in baselin...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.07.049
更新日期:2005-12-16 00:00:00