Abstract：
:Haploids and double haploids are important resources for studying recessive traits and have large impacts on crop breeding, but natural haploids are rare in animals. Mammalian haploids are restricted to germline cells and are occasionally found in tumours with massive chromosome loss. Recent success in establishing haploid embryonic stem (ES) cells in medaka fish and mice raised the possibility of using engineered mammalian haploid cells in genetic studies. However, the availability and functional characterization of mammalian haploid ES cells are still limited. Here we show that mouse androgenetic haploid ES (ahES) cell lines can be established by transferring sperm into an enucleated oocyte. The ahES cells maintain haploidy and stable growth over 30 passages, express pluripotent markers, possess the ability to differentiate into all three germ layers in vitro and in vivo, and contribute to germlines of chimaeras when injected into blastocysts. Although epigenetically distinct from sperm cells, the ahES cells can produce viable and fertile progenies after intracytoplasmic injection into mature oocytes. The oocyte-injection procedure can also produce viable transgenic mice from genetically engineered ahES cells. Our findings show the developmental pluripotency of androgenentic haploids and provide a new tool to quickly produce genetic models for recessive traits. They may also shed new light on assisted reproduction.
journal_name
Naturejournal_title
Natureauthors
Li W,Shuai L,Wan H,Dong M,Wang M,Sang L,Feng C,Luo GZ,Li T,Li X,Wang L,Zheng QY,Sheng C,Wu HJ,Liu Z,Liu L,Wang L,Wang XJ,Zhao XY,Zhou Qdoi
10.1038/nature11435subject
Has Abstractpub_date
2012-10-18 00:00:00pages
407-11issue
7420eissn
0028-0836issn
1476-4687pii
nature11435journal_volume
490pub_type
杂志文章相关文献
NATURE文献大全abstract：:Surface gravity is a basic stellar property, but it is difficult to measure accurately, with typical uncertainties of 25 to 50 per cent if measured spectroscopically and 90 to 150 per cent if measured photometrically. Asteroseismology measures gravity with an uncertainty of about 2 per cent but is restricted to relati...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12419
更新日期：2013-08-22 00:00:00
abstract：:Proviruses of avian leukosis virus (ALV) are located in the vicinity of a putative cellular oncogene (c-myc) in ALV-induced bursal lymphomas. Enhanced expression of c-myc occurs in association with proviruses found in any of three configurations: (I) on the 5' side ('upstream') of c-myc in the same transcriptional ori...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/295209a0
更新日期：1982-01-21 00:00:00
abstract：:Expansion of anthropogenic noise and night lighting across our planet1,2 is of increasing conservation concern3-6. Despite growing knowledge of physiological and behavioural responses to these stimuli from single-species and local-scale studies, whether these pollutants affect fitness is less clear, as is how and why ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2903-7
更新日期：2020-11-01 00:00:00
abstract：:DNA methylation has a profound impact on genome stability, transcription and development. Although enzymes that catalyse DNA methylation have been well characterized, those that are involved in methyl group removal have remained elusive, until recently. The transformative discovery that ten-eleven translocation (TET) ...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/nature12750
更新日期：2013-10-24 00:00:00
abstract：:A CENTRAL assumption about long-term potentiation in the hippocampus is that the two classes of glutamate-receptor ion channel, the N-methyl-D-aspartate (NMDA) and the kainate/quisqualate (non-NMDA) subtypes, are co-localized at individual excitatory synapses. This assumption is important because of the perceived inte...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/341230a0
更新日期：1989-09-21 00:00:00
abstract：:During fertilization, an egg and a sperm fuse to form a new embryo. Eggs develop from oocytes in a process called meiosis. Meiosis in human oocytes is highly error-prone, and defective eggs are the leading cause of pregnancy loss and several genetic disorders such as Down's syndrome. Which genes safeguard accurate pro...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature14568
更新日期：2015-08-13 00:00:00
abstract：:Antifreeze proteins provide fish with protection against the freezing effect of polar environments by binding to ice surfaces and inhibiting growth of ice crystals. We present the X-ray crystal structure at 1.5 A resolution of a lone alpha-helical antifreeze protein from winter flounder, which provides a detailed look...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/375427a0
更新日期：1995-06-01 00:00:00
abstract：:Periodic somite segmentation in vertebrate embryos is controlled by the 'segmentation clock', which consists of numerous cellular oscillators. Although the properties of a single oscillator, driven by a hairy negative-feedback loop, have been investigated, the system-level properties of the segmentation clock remain l...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature04861
更新日期：2006-06-08 00:00:00
abstract：:Organic photovoltaic cells are now approaching commercially viable efficiencies, particularly for applications that make use of their unique potential for flexibility and semitransparency1-3. However, their reliability remains a major concern, as even the most stable devices reported so far degrade within only a few y...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1544-1
更新日期：2019-09-01 00:00:00
abstract：:The interplay between magnetic fields and interacting particles can lead to exotic phases of matter that exhibit topological order and high degrees of spatial entanglement. Although these phases were discovered in a solid-state setting, recent innovations in systems of ultracold neutral atoms-uncharged atoms that do n...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature22811
更新日期：2017-06-21 00:00:00
abstract：:Chick embryo fibroblasts (CEF) infected with avian sarcoma virus become rapidly transformed as a result of expression of the viral src gene in the form of a single polypeptide of molecular weight 60,000 (pp60src) with protein kinase activity and suggested preferential association with the plasma membrane. Studies with...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/287241a0
更新日期：1980-09-18 00:00:00
abstract：:Photoinduced electron transfer (PET) is a phenomenon whereby the absorption of light by a chemical species provides an energetic driving force for an electron-transfer reaction1-4. This mechanism is relevant in many areas of chemistry, including the study of natural and artificial photosynthesis, photovoltaics and pho...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2131-1
更新日期：2020-04-01 00:00:00
abstract：:Major histocompatibility complex (MHC) class I and class II molecules bind immunogenic peptides and present them to lymphocytes bearing the alpha beta T-cell antigen receptor (TCR). An analogous antigen-presenting function also has been proposed for the non-MHC-encoded CD1 molecules, a family of non-polymorphic, beta ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/372691a0
更新日期：1994-12-15 00:00:00
abstract：:The carotid body type I cell contains amines and has features, both morphological and cytochemical, which indicate that it may also produce a peptide. Many regulatory peptides are now known to be present in both central and peripheral tissues. In the periphery these neuropeptides occur in both classical endocrine (APU...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/284269a0
更新日期：1980-03-20 00:00:00
abstract：: ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/d41586-018-07595-4
更新日期：2018-12-01 00:00:00
abstract：:The inner ear is capable of highly selective frequency discrimination. This is achieved not only by the travelling wave of the basilar membrane in the cochlear partition, but also by the active participation of nonlinear and vulnerable elements that enhance frequency selectivity. It has been shown that isolated mammal...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/342814a0
更新日期：1989-12-14 00:00:00
abstract：:A central aspect of neuronal function is how each nerve cell translated synaptic input into a sequence of action potentials that carry information along the axon, coded as spike frequency. When transduction from a graded depolarizing input to spikes is studied by injecting a depolarizing current, there is often a rema...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/336379a0
更新日期：1988-11-24 00:00:00
abstract：:Fluctuations in the counting rate of photons originating from uncorrelated point sources become, within the coherently illuminated area, slightly enhanced compared to a random sequence of classical particles. This phenomenon, known in astronomy as the Hanbury Brown-Twiss effect, is a consequence of quantum interferenc...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature00911
更新日期：2002-07-25 00:00:00
abstract：:Diffuse gliomas are the most common malignant brain tumours in adults and include glioblastomas and World Health Organization (WHO) grade II and grade III tumours (sometimes referred to as lower-grade gliomas). Genetic tumour profiling is used to classify disease and guide therapy1,2, but involves brain surgery for ti...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-0882-3
更新日期：2019-01-01 00:00:00
abstract：:Inositol-1,4,5-trisphosphate receptors (InsP(3)Rs) and ryanodine receptors (RyRs) are tetrameric intracellular Ca(2+) channels. In each of these receptor families, the pore, which is formed by carboxy-terminal transmembrane domains, is regulated by signals that are detected by large cytosolic structures. InsP(3)R gati...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10751
更新日期：2012-01-29 00:00:00
abstract：:DNA sequences have been analysed using models, such as an n-step Markov chain, that incorporate the possibility of short-range nucleotide correlations. We propose here a method for studying the stochastic properties of nucleotide sequences by constructing a 1:1 map of the nucleotide sequence onto a walk, which we term...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/356168a0
更新日期：1992-03-12 00:00:00
abstract：:The shape of the human erythrocyte depends on intracellular ATP content and echinocytic erythrocyte ghosts obtained in the presence of Mg-ATP acquire a diskocytic shape after incubation at 37 degrees C; however, agreement is lacking about the molecular basis of the shape changes. The suggestion that phosphorylation of...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/307376a0
更新日期：1984-01-26 00:00:00
abstract：:Several functionally important genetic elements (such as the TATA box, mRNA splice sequences, poly(A) addition signal) were first detected as short segments of unexplained sequence homology within non-coding regions of different genes. A short region of unknown sequence in the intron between the human J kappa and C ka...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/304447a0
更新日期：1983-08-04 00:00:00
abstract：:'Long' gamma-ray bursts (GRBs) are commonly accepted to originate in the explosion of particularly massive stars, which give rise to highly relativistic jets. Inhomogeneities in the expanding flow result in internal shock waves that are believed to produce the gamma-rays we see. As the jet travels further outward into...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature03934
更新日期：2005-08-18 00:00:00
abstract：:An emerging concept in the field of cancer biology is that a rare population of 'tumour stem cells' exists among the heterogeneous group of cells that constitute a tumour. This concept, best described with human leukaemia, indicates that stem cell function (whether normal or neoplastic) might be defined by a common se...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01572
更新日期：2003-05-15 00:00:00
abstract：:Analysis of the genes that control the early events of Drosophila embryogenesis is providing details of the molecular processes underlying the positional specification of cells. There are two distinct phases: the first precedes the cellularization of the blastoderm embryo and is associated with a cascade of interactio...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/335025a0
更新日期：1988-09-01 00:00:00
abstract：:Skeletal muscles consist of motor units which may differ considerably in contractile properties and types of usage. Some units participate mainly in relatively rare, quick movements and contract rapidly and are easily fatigued (type FF); others contribute to the maintenance of posture and hence contract slowly and are...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/314164a0
更新日期：1985-03-14 00:00:00
abstract：:Much interest has recently centred on the properties of peptides that modulate the excitability of nerve cells. Such compounds include the undecapeptide substance P, which is particularly well established as an excitatory neurotransmitter, and we examine here its effects on magnocellular cholinergic neurones taken fro...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/315498a0
更新日期：1985-06-06 00:00:00
abstract：:Intracellular Ca2+ signalling processes are fundamental to muscle contraction, neurotransmitter release, cell growth and apoptosis. Release of Ca2+ from the intracellular stores is supported by a series of ion channels in sarcoplasmic or endoplasmic reticulum (SR/ER). Among them, two isoforms of the trimeric intracell...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature19767
更新日期：2016-10-27 00:00:00
abstract：:Cell competition-the sensing and elimination of less fit 'loser' cells by neighbouring 'winner' cells-was first described in Drosophila. Although cell competition has been proposed as a selection mechanism to optimize tissue and organ development, its evolutionary generality remains unclear. Here, by using live imagin...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1199-y
更新日期：2019-05-01 00:00:00
