Abstract:
:Leptin is considered an adipokine, however, cultured myocytes have also been found to release leptin. Therefore, as proof-of-concept we investigated if human skeletal muscle synthesized leptin by measuring leptin in skeletal muscle biopsies. Following this, we quantified human skeletal muscle and adipose tissue leptin release in vivo. We recruited 16 healthy male human participants. Catheters were inserted into the femoral artery and vein draining skeletal muscle, as well as an epigastric vein draining the abdominal subcutaneous adipose tissue. By combining the veno-arterial differences in plasma leptin with measurements of blood flow, leptin release from both tissues was quantified. To induce changes in leptin, the participants were infused with either saline or adrenaline in normo-physiological concentrations. The presence of leptin in skeletal muscle was confirmed by western blotting. Leptin was released from leg skeletal muscle (50.6 ± 12 ng min(-1)) and the pattern of release was different from subcutaneous adipose tissue. Moreover, during adrenaline infusion the leptin release from leg skeletal muscle was strongly suppressed (20.5 ± 7.9 ng min(-1), p<0.017), whereas the release from fat was unaltered. During saline infusion the adipose tissue release averaged 0.8 ± 0.3 ng min(-1) 100g tissue(-1) whereas skeletal muscle release was 0.5 ± 0.1 ng min(-1) 100g tissue(-1). In young healthy humans, skeletal muscle contribution to whole body leptin production could be substantial given the greater mass of muscle compared to fat. An understanding of the role that leptin plays in skeletal muscle metabolism may prove important in light of several late-phase trials with recombinant leptin as an anti-obesity drug.
journal_name
Cytokinejournal_title
Cytokineauthors
Wolsk E,Mygind H,Grøndahl TS,Pedersen BK,van Hall Gdoi
10.1016/j.cyto.2012.08.021subject
Has Abstractpub_date
2012-12-01 00:00:00pages
667-73issue
3eissn
1043-4666issn
1096-0023pii
S1043-4666(12)00662-Xjournal_volume
60pub_type
杂志文章相关文献
CYTOKINE文献大全abstract:BACKGROUND:Soy products contain several compounds with anti-inflammatory properties like genistein and daidzein which reported to act through different pathways. Present study conducted considering the inconsistent results and lack of any comprehensive review regarding randomized controlled trials which assess the effe...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155282
更新日期:2020-12-01 00:00:00
abstract::IFN-gamma signalling involves the Jak-STAT pathway. However, several hypothesis have been proposed where the receptor and its ligand itself took an active role within the cell. Using a quantitative immunogold approach, we found that both IFN-gamma and its receptor are rapidly internalized and translocated in the nucle...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1999.0606
更新日期:2000-06-01 00:00:00
abstract::Cytokine induction following stimulation by endotoxin (LPS) was investigated in human peripheral whole blood. Blood cells were suspended in autologous plasma diluted in basal medium (BM-plasma) or Compound Sodium Lactate Solution (CSL-plasma), or in autologous serum diluted in CSL. Induction of interleukin 1 beta, int...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(94)90020-5
更新日期:1994-05-01 00:00:00
abstract::Typically, ovarian cancer remains restricted to the peritoneal cavity. Because of this unique localization, the study of ovarian cancer is particularly suitable for immune analysis and for the development of immunotherapy. Here we report that peritoneal fluid from patients with ovarian or other intra-abdominal cancers...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(92)90082-3
更新日期:1992-09-01 00:00:00
abstract::Growth related oncogene protein-alpha (GRO-alpha) is a member of C-X-C chemokine and plays an important role in inflammatory responses. Expression of GRO gene family is regulated by a number of factors at both transcriptional and posttranscriptional levels. In the present study, we have addressed the possible regulati...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/s1043-4666(03)00271-0
更新日期:2003-11-07 00:00:00
abstract::We investigated inflammatory cytokine response in chronic depressed patients during abdominal surgery. Twenty-five major depressed patients (Group D) and twenty-five patients (Group C) as the control were studied. Plasma interleukin 6 (IL-6), interleukin 8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) concentrat...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2000.0802
更新日期:2001-01-21 00:00:00
abstract::We have studied the effects of rhTGF-beta2, dexamethanosome (dex) and 1,25(OH)2D3 on human bone marrow stromal (HBMS) cells in long-term culture. A fraction on HBMS expressed type I collagen (Col I) and osteopontin, and transient treatment (48 h) with dex increased the number of alkaline phosphatase (ALP) positive cel...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1997.0209
更新日期:1997-08-01 00:00:00
abstract::The present study was aimed at defining the activity of human and mouse monocyte chemotactic protein-1 (MCP-1) on homologous and heterologous mononuclear phagocytes. Human natural and recombinant MCP-1 and mouse natural MCP-1/JE were tested as chemoattractants on human blood monocytes and mouse peritoneal macrophages....
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/1043-4666(94)90004-3
更新日期:1994-01-01 00:00:00
abstract::In the atopic allergic individual, challenge with allergen elicits manifestations of both the humoral (IgE-mediated or early-phase reaction) and the cell-mediated immune response (late-phase reaction). Detailed study of late-phase cell-mediated events is confounded by the effects of the earlier IgE-mediated response w...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2004.07.001
更新日期:2004-10-21 00:00:00
abstract::The in vivo influence of recombinant human erythropoietin (rhEpo) and iron on human neutrophil (PMN) antimicrobial function was assessed. A total of 21 preterm infants were randomized to receive either 200 U/kg/other day of rHuEPO+12 mg/kg/day of iron (EPO+high Fe, seven infants) or 200 U/kg/other day of rhEPO+4 mg/kg...
journal_title:Cytokine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1006/cyto.1998.0393
更新日期:1999-01-01 00:00:00
abstract::These experiments were designed to investigate the effects of IL-17 upon the phenotype and function of human Natural Killer (NK) cells. Peripheral blood mononuclear cells from healthy subjects were cultured in the presence or absence of different combinations of IL-17s and changes in relative numbers and cell surface ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2013.02.005
更新日期:2013-04-01 00:00:00
abstract::Enteroviruses are associated with chronic inflammatory and autoimmune diseases in humans. In these conditions, the cytokine network is supposed to have an important role in inflammation and modulation of the (auto)immune response. In the present study, we demonstrate that coxsackie virus B4 and poliovirus type 1 induc...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2000.0786
更新日期:2000-12-01 00:00:00
abstract::Hepatitis C virus (HCV) chronic infection causes severe cellular immune dysfunction. Here, we investigated the production of Th17-associated cytokines by peripheral blood mononuclear cells (PBMCs) of untreated patients with HCV, patients presenting an early virologic response (EVR) after 12weeks of treatment with inte...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2017.09.015
更新日期:2018-02-01 00:00:00
abstract::In atherosclerotic plaques, macrophages (MAC) and smooth muscle cells (SMC) frequently reside in close proximity and resistin (Rs) and fractalkine (Fk) are present at increased levels, resistin being associated with CD68 macrophages and fractalkine predominantly associated with intimal SMC; however, their role in this...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2016.04.019
更新日期:2016-07-01 00:00:00
abstract::Azidothymidine (AZT)-induced anemia in mice can be reversed by the administration of IGF-IL-3 (fusion protein of insulin-like growth factor II (IGF II) and interleukin 3). Although interleukin 3 (IL-3) and erythropoietin (EPO) are known to act synergistically on hematopoietic cell proliferation in vitro, injection of ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.1029
更新日期:2002-04-07 00:00:00
abstract::Along with several other aspects of Chagas disease, the mechanisms responsible for the different clinical outcomes observed in chronic infected individuals have not yet been clarified. It is believed that the host immune response to the parasite plays an important role in the development of the pathology. Therefore, t...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2012.01.008
更新日期:2012-05-01 00:00:00
abstract::Muscle wasting is a decline in skeletal muscle mass and function that is associated with aging, obesity, and a spectrum of pathologies including cancer. Cancer-associated wasting not only reduces quality of life, but also directly impacts cancer mortality, chemotherapeutic efficacy, and surgical outcomes. There is an ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2017.11.006
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:Egypt has a high prevalence of hepatitis C virus (HCV) infection. Limitations of the current HCV treatment in children are low rate of sustained virological response, significant side effects and high expenses, making prediction of treatment response crucial. AIM:This study aimed to investigate association ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.04.005
更新日期:2015-10-01 00:00:00
abstract::Interleukin (IL)-6, a multifunctional cytokine, is widely used as an index for illnesses such as inflammatory and autoimmune disorders, coronary artery disease, neurological disease, and gestational problems. It is thus very important to be able to precisely quantify the level of IL-6 for disease diagnosis and any sub...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2010.04.004
更新日期:2010-07-01 00:00:00
abstract::Although physical therapy has been shown to be an effective method for treatment of rheumatoid arthritis, a thorough investigation on the impact of mechanical signals upon the complex cytokine network associated with pathogenesis has not yet been conducted. In the current study, our research group investigated the eff...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2010.03.015
更新日期:2010-07-01 00:00:00
abstract::Interleukin 1alpha (IL-1alpha), a pleiotropic cytokine with multiple anti-tumour activities, has been investigated in our laboratory for its potential to serve as an immunotherapeutic agent. In the present study, an attempt was made to direct IL-1alpha to macrophages, in order to induce their immunoregulatory activiti...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2000.0775
更新日期:2000-11-01 00:00:00
abstract:OBJECTIVE:Brain abscess develops in response to a parenchymal infection with pyogenic bacteria. Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) are the most crucial pro-inflammatory cytokines with both beneficial and destructive properties for the central nervous system. The present study evaluated the assoc...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.07.009
更新日期:2015-09-01 00:00:00
abstract::Encephalitic listeriosis in sheep is a life-threatening disease. However, little is known about the cytokine response and their predictive value in this disease. The aim of present study was to assess the prognostic significance of Tumor Necrosis Factor-α (TNF-α), Interleukin-12(p40) (IL-12 p40), Interleukin-6 (IL-6),...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.03.004
更新日期:2015-06-01 00:00:00
abstract::Increasing evidence shows that inflammation plays critical roles in the tumorigenesis of bladder cancer. Fibroblast growth factor 12 (FGF12), a kind of inflammatory cytokine, is located in the region of 3q28 that has been demonstrated to be a bladder cancer risk locus by genome wide association study (GWAS). In this s...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155294
更新日期:2020-12-01 00:00:00
abstract:INTRODUCTION:Resveratrol has been reported to alleviate inflammatory responses and oxidative stress in mesangial cells and in several types of renal injury in animal models. Previously, the active resveratrol derivatives from the roots of Vitis thunbergii Sieb. & Zucc. (Vitaceae) were shown to have significant anti-pla...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2018.10.008
更新日期:2019-01-01 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) is a molecule with plethora of functions such as regulation of immune response, hormone-like, enzymatic and chaperone-like activity. Further, MIF is a major participant in glucose homeostasis since it is an autocrine stimulator of insulin secretion. MIF absence in male knoc...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2014.05.013
更新日期:2014-09-01 00:00:00
abstract::Expression of GPCR fatty acid sensor/receptor genes in adipocytes is modulated by inflammatory mediators, particularly IL-1β. In this study we examined whether the IL-1 gene superfamily member, IL-33, also regulates expression of the fatty acid receptor genes in adipocytes. Human fat cells, differentiated from preadip...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2018.05.008
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:Three tear supplements were compared for their effects on the signs, symptoms and inflammatory status of subjects with dry eye disease. Assessments were made before and after both 2 and 4 weeks of treatment. METHODS:In this masked, randomized, 3-way crossover trial, eighteen dry eye subjects were recruited. At...
journal_title:Cytokine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.cyto.2018.02.009
更新日期:2018-05-01 00:00:00
abstract::Cell death and innate immunity are ancient evolutionary conserved processes that utilize a dazzling number of related molecular effectors and parallel signal transduction mechanisms. The investigation of the molecular mechanisms linking the sensing of a danger signal (pathogens or tissue damage) to the induction of an...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2008.07.015
更新日期:2008-09-01 00:00:00
abstract::Chemokines are small chemoattractant cytokines involved in normal and pathological immune processes. Although extensive nucleotide sequence data are available for human and murine chemokine cDNA sequences, very few data are currently available regarding rhesus macaque sequences. To increase our understanding of immune...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2002.0875
更新日期:2002-05-07 00:00:00