当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides).

Cloning, characterization, and expression analysis of a thioredoxin from orange-spotted grouper (Epinephelus coioides).

Abstract：

:Thioredoxins (TRXs) are a family of small, highly conserved proteins that are essential for the maintenance of cellular homeostasis. In this study, a thioredoxin gene was cloned from orange-spotted grouper, Epinephelus coioides (designated as Ec-TRX). The full-length cDNA of Ec-TRX was comprised of 767bp with a 327bp open reading frame that encodes a putative protein of 108 amino acids. Quantitative real-time PCR analysis revealed that the Ec-TRX mRNA was distributed abundantly in grouper, E. coioides skin and liver, and the expression in liver was up-regulated after viral challenge with Singapore grouper iridovirus (SGIV). Recombinant Ec-TRX (rEc-TRX) was expressed in Escherichia coli BL21 (DE3) and purified for mouse anti-Ec-TRX serum preparation. The rEc-TRX fusion protein was demonstrated to possess the expected redox activity in enzymatic analysis, and scavenge free radicals and protect supercoiled DNA from oxidative damage induced by a metal-ion catalyzed oxidation reaction. Subcellular localization revealed that Ec-TRX was distributed in both cytoplasm and nucleus. Overexpression of Ec-TRX in grouper spleen (GS) cells could promote the growth of GS cells and inhibit the replication of SGIV. These results suggest that Ec-TRX could function as an important antioxidant in a physiological context, and perhaps is involved in the responses to viral challenge.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Wei J,Guo M,Ji H,Yan Y,Ouyang Z,Huang X,Hang Y,Qin Q

doi

10.1016/j.dci.2012.04.011

subject

Has Abstract

pub_date

2012-09-01 00:00:00

pages

108-16

issue

1

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(12)00084-5

journal_volume

38

pub_type

杂志文章

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