Abstract:
BACKGROUND:The actions of endogenous opioid peptides are mediated by 3 main classes of opioid receptors; mu (MOR), kappa (KOR), and delta (DOR). METHODS:We developed an absolute quantitative real-time reverse transcriptase PCR (AQ-rt-RT-PCR) assay to quantify MOR, DOR, and KOR mRNA in 22 human tissues. RESULTS:MOR mRNA was greatly enriched (12-20×10(6)copies/μg) in the cerebellum, nucleus accumbens, and caudate nucleus; moderate (6×10(6)copies/μg) in the dorsal root ganglion, spinal cord, and adrenal gland; low (2×10(4)copies/μg) in the pancreas and small intestine; and absent in the lung, spleen, kidney, heart, skeletal muscle, liver, and thymus. High levels (>8.8×10(6)copies/μg) of DOR mRNA were expressed in the brain and dorsal root ganglion; moderate (1.5×10(6)copies/μg) in the adrenal gland and pancreas; low (2×10(4)-6.5×10(5)copies/μg in the cerebellum, spinal cord, small intestine, skeletal muscle, thymus, lung, and kidney); and very low (3.8×10(3)copies/μg) in the heart. DOR mRNA was not detected in the spleen or liver. KOR mRNA was moderate (1×10(6)copies/μg) in brain regions and dorsal root ganglion, but low (1.6-7×10(5)copies/μg) in the cerebellum, temporal lobe and all other peripheral tissues. CONCLUSIONS:Our data demonstrate that the AQ-rt-RT-PCR is a highly reproducible and precise method to study the expression of opioid receptors in various tissues and under different disease conditions.
journal_name
Drug Alcohol Dependjournal_title
Drug and alcohol dependenceauthors
Peng J,Sarkar S,Chang SLdoi
10.1016/j.drugalcdep.2012.01.013subject
Has Abstractpub_date
2012-08-01 00:00:00pages
223-8issue
3eissn
0376-8716issn
1879-0046pii
S0376-8716(12)00030-0journal_volume
124pub_type
杂志文章abstract:BACKGROUND:Prominent addiction models posit that automatically activated approach/avoidance tendencies play a critical role in addiction. Nevertheless, only a limited number of studies have actually documented the relationship between relapse and automatically activated approach/avoidance tendencies. We compared automa...
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journal_title:Drug and alcohol dependence
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journal_title:Drug and alcohol dependence
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章,随机对照试验
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journal_title:Drug and alcohol dependence
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1999-12-01 00:00:00
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doi:10.1016/j.drugalcdep.2010.10.022
更新日期:2011-06-01 00:00:00
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journal_title:Drug and alcohol dependence
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doi:10.1016/s0376-8716(98)00022-2
更新日期:1998-04-01 00:00:00
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journal_title:Drug and alcohol dependence
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doi:10.1016/j.drugalcdep.2005.11.014
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章
doi:10.1016/0376-8716(79)90013-9
更新日期:1979-05-01 00:00:00
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章
doi:10.1016/j.drugalcdep.2018.04.032
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章,随机对照试验
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章
doi:10.1016/j.drugalcdep.2011.03.016
更新日期:2011-11-01 00:00:00
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journal_title:Drug and alcohol dependence
pub_type: 杂志文章,多中心研究
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更新日期:2017-06-01 00:00:00
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journal_title:Drug and alcohol dependence
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doi:10.1016/j.drugalcdep.2014.01.007
更新日期:2014-05-01 00:00:00