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Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro.

Abstract：

:The petroleum ether extract of neem oil and its four fractions separated by column chromatography was diluted at different concentrations with liquid paraffin. The acaricidal bioassay was conducted using a dipping method. The results indicated that the median lethal concentration (LC50) of the petroleum ether extract (at the concentration of 500.0ml/l) was 70.9ml/l, 24h after treatment. At concentrations of 500.0, 250.0, 125.0, 62.5 and 31.2ml/l, the median lethal times (LT50) of the petroleum ether extract were 8.7, 8.8, 10.8, 11.5 and 13.1h, respectively. Thin-layer chromatography (TLC) showed that the petroleum ether extract of neem oil separated into four fractions (F1-F4). Acaricidal activity of 68.3% and 100.0% in the F2 and F4 was confirmed. These results suggest that petroleum ether extracts of neem oil and its four fractions possess useful acaricidal activity in vitro.

journal_name

Exp Parasitol

journal_title

Experimental parasitology

authors

Deng Y,Shi D,Yin Z,Guo J,Jia R,Xu J,Song X,Lv C,Fan Q,Liang X,Shi F,Ye G,Zhang W

doi

10.1016/j.exppara.2012.02.007

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

475-7

issue

4

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(12)00045-8

journal_volume

130

pub_type

杂志文章

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