Abstract：
:The present research examines the effects of 4-nonylphenol (4-NP) on mouse primary calvarial osteoblasts (COBs). Incubation of the cells with 4-NP at 10(-5)M and 10(-6)M striking decreased osteoblasts viability and phosphatidylserine (PS) exposure, measured by Annexin V, was greatly enhanced. In addition, an up-regulation of Bax/Bcl2 ratio with a drop in ΔΨm and an increase of cleaved caspase 9 and 3 was found, suggesting that the alkylphenol induced osteoblast death via the mitochondrial-dependent apoptotic pathway. Interestingly, treatment with 4-NP was also able to increase cleaved caspase 8 in parallel with the truncated active Bid (t-Bid) suggesting that 4-NP-mediated apoptosis depends on cross talk between the extrinsic and intrinsic pathways. It is of relevance, that the apoptotic effects of 4-NP overcame 17-β-Estradiol (17-β E(2)) induced-survival on osteoblasts. Also, the alkylphenol interfered with 17-β E(2) regulated estrogen receptors expression.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Sabbieti MG,Agas D,Palermo F,Mosconi G,Santoni G,Amantini C,Farfariello V,Marchetti Ldoi
10.1016/j.tox.2011.10.014subject
Has Abstractpub_date
2011-12-18 00:00:00pages
334-41issue
2-3eissn
0300-483Xissn
1879-3185pii
S0300-483X(11)00453-7journal_volume
290pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract：:Pentachlorophenol (PCP) is an inhibitor of phenol-sulfotransferases and has been used to ascertain the role of sulfation in toxicology. Recently, molybdate has been shown to inhibit the sulfation of various chemicals by decreasing hepatic concentrations of the cosubstrate, 3'-phosphoadenosine 5'-phosphosulfate (PAPS)....
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(00)00159-1
更新日期：2000-04-20 00:00:00
abstract：:Rats were treated with a single dose of thallium acetate (32 mg/kg i.p.) and the antidotal effect of D-penicillamine and prussian blue given alone or in combination was assessed by means of evaluation of the thallium-induced cerebellar histological lesions. After thallium poisoning (24 h), antidotes were administered ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)90129-5
更新日期：1994-03-25 00:00:00
abstract：:Tetrahydrocannabinol (THC), a psychoactive component of marijuana, is known to exert cytotoxicity in immune cells. In the present study, we examined the cytotoxicity of Δ⁸-THC in mouse macrophage J774-1 cells and a possible involvement of cannabinoid receptors and stress-responsive mitogen-activated protein kinases (M...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2013.10.007
更新日期：2013-12-15 00:00:00
abstract：:Because occupational hazards of small-scale industries were severe and the occupational health management lagged behind in Shanghai, we selected some districts as experimental units to explore a model of community management and service for the occupational health in small-scale industries for controlling occupational...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.01.019
更新日期：2004-05-20 00:00:00
abstract：:Isoniazid is a widely used drug for the treatment of tuberculosis, but hepatotoxicity is a major concern during treatment. Thiopronin contains an SH-group and is generally considered an antioxidant. The aim of the present study was to investigate the effects of thiopronin during liver injury and DNA damage induced by ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2009.08.006
更新日期：2009-10-29 00:00:00
abstract：:In several separate experiments neonatal rats were intubated daily with 9, 27 or 81 mg lead acetate/kg of body weight throughout their 3-week postnatal period of development. Based on average body weights, the total daily lead intake was 0.156, 0.454 or 1.384 mg lead per animal, respectively (in addition to normal lea...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(75)90115-8
更新日期：1975-11-01 00:00:00
abstract：:To evaluate hydroperoxide generation as a potential mechanism of cyanide neurotoxicity, mice were treated with KCN (7 mg/kg, subcutaneously (s.c.)) and the level of lipid peroxidation (expressed as conjugated dienes) was measured later in various organs. Brain showed elevated conjugated diene levels after cyanide but ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)90221-6
更新日期：1994-04-18 00:00:00
abstract：:Cytochrome P450 (CYP) 2A13 is mainly expressed in the respiratory system and has the ability to metabolize aflatoxin B(1) (AFB(1)). However, the role of CYP2A13-mediated AFB(1) metabolism and its consequences in human lung epithelial cell is not clear. Therefore, the objectives of this study were to investigate the si...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2012.06.010
更新日期：2012-10-28 00:00:00
abstract：:Experiments were designed to assess the mechanisms of diisopropyl fluorophosphate (DFP)-induced changes in thermoregulation of the rat. In one study, male rats of the Long-Evans strain were injected with DFP (s.c.) at doses ranging from 0 to 2.0 mg/kg while maintained at an ambient temperature (Ta) of 20--24 degrees C...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(91)90159-x
更新日期：1991-03-25 00:00:00
abstract：:Butachlor is a systemic herbicide widely applied on rice, tea, wheat, beans and other crops; however, it concurrently exerts toxic effects on beneficial organisms like earthworms, aquatic invertebrates and other non-target animals including humans. Owing to the associated risk to humans, this chloroacetanilide class o...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2012.07.014
更新日期：2012-12-08 00:00:00
abstract：:This study is aimed to investigate the inflammation and neurological dysfunction induced by tetrachloro-p-benzoquinone (TCBQ) through Toll-like receptor 4 (TLR4) signaling. We also investigated the protective role of melatonin as an antioxidant and anti-inflammatory agent. In vitro model was established by rat pheochr...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2017.02.015
更新日期：2017-04-15 00:00:00
abstract：:Nonylphenol (NP) has been identified at low levels in surface waters throughout North America. This industrial chemical is primarily used for the production of certain non-ionic surfactants, and has been reported to have weak estrogen-like activity. As estrogen has immunoregulatory properties and is crucial for normal...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2003.11.009
更新日期：2004-03-15 00:00:00
abstract：:An experimental approach was taken to the question of dose-response curves for chemical carcinogenesis, using DNA damage as a biomarker. Female rats were give 13 different doses of 1,2-dimethylhydrazine (from 1.4 to 135,000 micrograms/kg) and the subsequent hepatic DNA damage was determined by the alkaline elution tec...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(96)03478-6
更新日期：1996-12-02 00:00:00
abstract：:The effects of monosodium L-glutamate (MSG) on the brain of mice were examined by light-microscopy under the intended conditions of use of this material. Basal diet containing 5, 10 or 15% w/w MSG was fed to pregnant, lactating and weanling mice ad libitum, resulting in the ingestion of 14.0, 42.8 or 42.0 g/kg body wt...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(78)90014-8
更新日期：1978-04-01 00:00:00
abstract：:Many models have been developed to predict the combined effect of drugs and chemicals. Most models are classified into two additive models: independent action (IA) and concentration addition (CA). It is generally considered if the modes of action of chemicals are similar then the combined effect obeys CA; however, man...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2015.06.008
更新日期：2015-09-01 00:00:00
abstract：:It is reported that gambogic acid (GA), the main active compound of gamboge which is a dry resin extracted from Garcinia hanburyi tree, has potent antitumor activity both in vivo and in vitro. Activation of mitochondrial apoptotic pathway in cancer cells is one effective therapy for cancer treatment. In the present st...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2009.03.010
更新日期：2009-06-16 00:00:00
abstract：:The rodent Hershberger assay has been used predominantly by the pharmaceutical industry to evaluate androgenic and antiandrogenic chemicals for potential therapeutic use. However, this assay has not yet been formally validated and standardized for use in toxicology testing. There are many variations in the protocol us...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(01)00361-4
更新日期：2001-05-11 00:00:00
abstract：:To investigate the biological effects of terbium (Tb), male mice were intraperitoneally administered with TbCl3 at doses of 10, 50, or 250 mg Tb/kg. The Tb distribution in organs was determined after 18-20 h of injection by using a spectrofluorometer. The concentrations of Ca, Mg, Fe, and Zn in various organs were det...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(91)90181-y
更新日期：1991-02-11 00:00:00
abstract：:The question as to whether or not the hypotension observed as part of the effect of tricholorofluoromethane (FC11), dichlorofluoromethane (FC 12), dichlorotetrafluoroethane (FC 114) and methyl chloroform was due to a vasodepressor component of action, in addition to the previously documented depression in myocardial c...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(76)90048-2
更新日期：1976-03-01 00:00:00
abstract：:Dimethoate, azinphos-methyl, diazinon and pirimiphos-methyl, widely used organophosphorous insecticides, and benomyl, a benzimidazole fungicide, induce different cytotoxic effects on the human leukemia cell line HL-60. Among the insecticides tested, only azinphos and diazinon induced a dose-related inhibition of prote...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)90036-1
更新日期：1994-11-01 00:00:00
abstract：:p-Dichlorobenzene (p-DCB) is widely used as a moth repellent and a space deodorant. In mice pretreated with DL-buthionine sulfoximine (BSO; 2 mmol/kg or higher doses, i.p.), an inhibitor of glutathione (GSH) synthesis, administration of p-DCB (100-400 mg/kg, p.o.) resulted in dose-dependent hepatotoxicity as judged by...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)90028-0
更新日期：1994-11-01 00:00:00
abstract：:Measurement of lymphocyte proliferation to detect hypersensitivity to beryllium (Be-LPT) in vitro is done presently using a method based on tritiated thymidine incorporation. Although this method is sensitive it gives no information on cell viability or responding lymphocyte subsets. We have developed reliable and sim...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(99)00167-5
更新日期：2000-02-21 00:00:00
abstract：:Insulin and dexamethasone are potent inhibitors of apoptosis induced by transforming growth factor-beta1 (TGF-beta) in hepatoma cells. Using FTO-2B rat hepatoma cells, we determined whether the anti-apoptotic effects of these agents result from interference within or upstream of the TGF-beta-induced caspase cascade. A...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.06.020
更新日期：2004-11-15 00:00:00
abstract：:Life-threatening acute lung injury potentially occurs following high-level accidental exposures to phosgene gas. This situation was mirrored in rats exposed nose-only at 900-1000 mg phosgene/m(3)min. At this exposure level, previous studies on rats demonstrated sustained reflexively induced cardiopulmonary dysfunction...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2013.11.005
更新日期：2014-01-06 00:00:00
abstract：:Acute and subchronic toxicities of VRCTC-310, a combination product of crotoxin (CT) and cardiotoxin (CD), which has shown antitumor activity in vivo, have been studied in Beagle dogs. Single i.m. doses of 0.25, 0.5 and 1.0 mg/kg resulted in dose-dependent local muscular toxicity consisting of myofiber atrophy, inters...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)02995-7
更新日期：1995-05-05 00:00:00
abstract：:V79 Chinese hamster cells are being genetically engineered for stable expression of cytochromes P450 using a SV40 early promoter containing plasmid as the eukaryotic expression vector. V79 cells lack endogenous cytochrome P450 activity. Therefore, genetically engineered V79 cell lines are defined for the cytochrome P4...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/0300-483x(93)90063-x
更新日期：1993-10-05 00:00:00
abstract：:In living organisms both chemical agents and physical factors [16] may produce neurophysiological change that affects EEG activity. EEG signals are very suitable for non-invasive measurement of CNS reactions, but quite complicated equipment is necessary for measurement and analysis. We have implemented a system that p...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(88)90004-2
更新日期：1988-05-01 00:00:00
abstract：:Ochratoxin A (OTA) is a mycotoxin produced by several fungi which grow on human food source material. Consumption of OTA is almost unavoidable. The consumption leads to low but detectable amounts of OTA in human blood. Risk assessment of OTA is based on studies performed either in animals or cultured cells. So far, ma...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2006.12.008
更新日期：2007-03-22 00:00:00
abstract：:The promoting effects of a combined exposure to two pollutants (NO2, O3 or H2SO4-aerosol) at near ambient levels on lung tumorigenesis induced by N-bis(2-hydroxypropyl) nitrosamine (BHPN) were investigated in male Wistar rats. The rats were given a single intraperitoneal injection of BHPN (0.5 g per kg body wt.) at 6 ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(92)90137-4
更新日期：1992-09-01 00:00:00
abstract：:This review presents a brief and non-comprehensive overview of a representative sampling of some of the broad array of toxicology-related learning, tutorial and information resources now becoming widely available to educators, health professionals, students and the general public in digital media and/or via the Intern...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(00)00344-9
更新日期：2001-01-12 00:00:00
