Abstract:
:V79 Chinese hamster cells are being genetically engineered for stable expression of cytochromes P450 using a SV40 early promoter containing plasmid as the eukaryotic expression vector. V79 cells lack endogenous cytochrome P450 activity. Therefore, genetically engineered V79 cell lines are defined for the cytochrome P450 isoform acquired upon cDNA mediated gene transfer. V79 cells have a longstanding tradition as indicator cells for various biological endpoints, e.g. mutation, chromosomal aberration, cytotoxicity. The genetically engineered V79 cells combine these useful biological endpoints with metabolic competence. In this sense, V79 cell lines genetically engineered for stable expression of cytochromes P450 present newly developed tools for studying and understanding metabolism related problems in toxicology and pharmacology.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Doehmer Jdoi
10.1016/0300-483x(93)90063-xsubject
Has Abstractpub_date
1993-10-05 00:00:00pages
105-18issue
1-3eissn
0300-483Xissn
1879-3185pii
0300-483X(93)90063-Xjournal_volume
82pub_type
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