Abstract：
:Aconitine (ACO) is a highly toxic diterpenoid alkaloid and known to exert the immunomodulatory action. However, whether it has any effects on ion currents in immune cells remains unknown. The effects of ACO and other related compounds on ion currents in Jurkat T-lymphocytes were investigated in this study. ACO suppressed the amplitude of delayed-rectifier K(+) current (I(K(DR))) in a time- and concentration-dependent manner. Margatoxin (100 nM), a specific blocker of K(V)1.3-encoded current, decreased the I(K(DR)) amplitude in these cells and the ACO-induced inhibition of I(K(DR)) was not reversed by 1-ethyl-2-benzimidazolinone (30 μM) or nicotine (10 μM). The IC(50) value for ACO-mediated inhibition of I(K(DR)) was 5.6 μM. ACO accelerated the inactivation of I(K(DR)) with no change in the activation rate of this current. Increasing the ACO concentration not only reduced the I(K(DR)) amplitude, but also accelerated the inactivation time course of the current. With the aid of minimal binding scheme, the inhibitory action of ACO on I(K(DR)) was estimated with a dissociation constant of 6.8 μM. ACO also shifted the inactivation curve of I(K(DR)) to a hyperpolarized potential with no change in the slope factor. Cumulative inactivation for I(K(DR)) was enhanced in the presence of ACO. In Jurkat cells incubated with amiloride (30 μM), the ACO-induced inhibition of I(K(DR)) remained unaltered. In RAW 264.7 murine macrophages, ACO did not modify the kinetics of I(K(DR)), although it suppressed I(K(DR)) amplitude. Taken together, these effects can significantly contribute to its action on functional activity of immune cells if similar results are found in vivo.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Wu SN,Chen BS,Lo YCdoi
10.1016/j.tox.2011.07.003subject
Has Abstractpub_date
2011-10-28 00:00:00pages
11-8issue
1eissn
0300-483Xissn
1879-3185pii
S0300-483X(11)00259-9journal_volume
289pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract：:For the first time, kinetic parameters of the effect of tacrine, an anti-cholinesterase inhibitor of therapeutic potential in Alzheimer's disease has been studied on human retinal acetyl-cholinesterase (AChE). Tacrine inhibited the AChE activity in a concentration dependent manner, the IC(50) being about 45 nM. The Mi...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(00)00177-3
更新日期：2000-05-19 00:00:00
abstract：:4-Bromo-2,5-dimethoxyphenethylamine (2C-B) is a psychoactive designer drug of abuse that is sold under the street names "Venus", "Bromo", "Erox", "XTC" or "Nexus". Concern has been raised because only little is known about its toxicity and metabolism in humans. In the present study we incubated 2C-B with human, monkey...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.07.004
更新日期：2005-01-05 00:00:00
abstract：:The development of poisons information centers has in general complied with the actual needs and conditions in the society, and a pragmatic approach and limited funds have been characteristic for these units. To operate in a satisfying way a poisons information center is dependent on two cornerstones-a well educated a...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2004.01.033
更新日期：2004-05-20 00:00:00
abstract：:Allyl acetate (AAC), allyl alcohol (AAL), and acrolein (ACR) are used in the manufacture of detergents, plastics, pharmaceuticals, and chemicals and as agricultural agents. A metabolic relationship exists between these chemicals in which allyl acetate is metabolized to allyl alcohol and subsequently to the highly reac...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2008.08.014
更新日期：2008-11-20 00:00:00
abstract：:When Xenopus laevis embryos were treated with fenitrothion, a substantial proportion failed to survive to hatching only when the concentration approached saturation. However, after exposure to 10 ppm of fenitrothion, most were abnormal and did not survive past feeding stage. Different embryonic stages were not equally...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(81)90026-3
更新日期：1981-01-01 00:00:00
abstract：:Many studies have demonstrated that polycyclic aromatic hydrocarbons adversely affect mature mammalian immune systems. However, little is known about the cellular mechanism(s) mediating this immunosuppression or the potential for these ubiquitous environmental chemicals to similarly compromise lymphocyte development (...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(92)90103-l
更新日期：1992-01-01 00:00:00
abstract：:Cell cycle aberration was immunohistochemically examined in relation to preneoplastic liver cell foci expressing glutathione S-transferase placental form (GST-P) at early stages of tumor-promotion in rats with thioacetamide (TAA), a hepatocarcinogen facilitating liver cell regeneration. Immunoexpression of p16(Ink4a) ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2012.05.018
更新日期：2012-09-28 00:00:00
abstract：:Indoor suspended particulate matter (SPM) consists of many different types of particles, the vast majority of which are less than 2.5 microm in diameter. An important question is how these particles, being inhalable, contribute to asthma and respiratory symptoms. One possibility is that these particles have an adjuvan...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(98)00079-1
更新日期：1998-08-21 00:00:00
abstract：:Clostridium difficile toxins A and B (TcdA and TcdB) belong to the class of large clostridial cytotoxins and inactivate by glucosylation some low molecular mass GTPases of the Rho-family (predominantly Rho, Rac and Cdc42), known as regulators of the actin cytoskeleton. TcdA and B also represent the main virulence fact...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2014.12.006
更新日期：2015-02-03 00:00:00
abstract：:Effects of methylmercury on the cell growth of mouse glioma in vitro were studied in relation to the microtubules as a possible target. The electron microscopic observation revealed that methylmercury added in culture medium specifically attacked microtubules in the cells at growth inhibitory concentration, while merc...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(80)90101-8
更新日期：1980-01-01 00:00:00
abstract：:The objective of this study was to observe whether 1.8 GHz microwaves (MW) (SAR, 3 W/kg) exposure can influence human lymphocyte DNA damage induced by ultraviolet ray C (UVC). The lymphocytes, which were from three young healthy donors, were exposed to 254 nm UVC at the doses of 0.25, 0.5, 0.75, 1.0, 1.5 and 2.0 J m(-...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2007.01.019
更新日期：2007-04-11 00:00:00
abstract：:Pharmacological evidence has accumulated showing that glucocorticoids and glucocorticoid receptor (GR) facilitate several responses to different drugs of abuse. Recent findings have attributed a prominent role to the mineralocorticoid receptor (MR) in modulating behavior during the addictive process. The purpose of th...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2014.10.002
更新日期：2014-12-04 00:00:00
abstract：:The objective of this study was to further evaluate the developmental toxicity of tributyltin chloride (TBTCl) in rats. Pregnant rats were given TBTCl by gastric intubation at a dose of 25, 50 or 100 mg/kg on days 7-9, days 10-12 or days 13-15 of pregnancy. A significant increase in the incidence of post-implantation ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(94)02920-p
更新日期：1995-02-27 00:00:00
abstract：:Double-stranded RNA-dependent protein kinase (PKR) is a component of signal transduction pathways mediating various stress signals including oxidative stress and endoplasmic reticulum (ER) stress and is suggested to be implicated in several neurodegenerative diseases. Cell death in neurodegenerative conditions has bee...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2019.02.012
更新日期：2019-04-15 00:00:00
abstract：:To evaluate the developmental toxicity of uranium, 5 groups of 20 pregnant Swiss mice were given by gavage daily doses of 0, 5, 10, 25 and 50 mg/kg of uranyl acetate dihydrate on gestational days 6-15. Cesarean sections were performed on all females on gestation day 18. Fetuses were examined for external, visceral, an...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(89)90181-9
更新日期：1989-04-01 00:00:00
abstract：:We studied the effect of O,S,S-trimethyl phosphorodithioate (OSS-Me) on macrophage function and the immune responses of both T- and B-cells using an in vitro system. The involvement of glutathione (GSH) in the bioactivation of OSS-Me to suppress such responses was investigated after either direct culture with spleen o...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(86)90155-1
更新日期：1986-04-01 00:00:00
abstract：OBJECTIVES:Infusion phlebitis is a common clinical problem associated with some antimicrobial agents. The pathomechanism of infusion phlebitis has not yet been elucidated, however, it has been proposed that chemical irritation of the endothelium leads to subsequent sterile inflammation with recruitment and migration of...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2005.09.014
更新日期：2006-01-20 00:00:00
abstract：:p-Chloro-m-cresol (PCMC) and p-chloro-m-xylenol (PCMX) are known to cause allergic contact dermatitis. For risk assessment of skin sensitizers, information on dose-response profiles in the induction and elicitation phases and cross-reactivity with analogous chemicals are important. In the non-radioactive local lymph-n...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(03)00161-6
更新日期：2003-08-28 00:00:00
abstract：:It has been suggested that the measurement of DNA-protein cross-links (DPCs) may be of value in the assessment of an individual's exposure to specific environmental insults. For a biomarker to be reliable, its results should be consistent and specific. In the present study, the precision and specificity of the K(+)-SD...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(96)03318-5
更新日期：1996-06-17 00:00:00
abstract：:Myopathy is among the well documented and the most disturbing adverse effects of statins. The underlying mechanism is still unknown. Mitochondrial dysfunction related to coenzyme Q10 decline is one of the proposed theories. The present study aimed to investigate the mechanism of atorvastatin-induced myopathy in rats. ...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2016.06.015
更新日期：2016-06-01 00:00:00
abstract：:The intravenous toxicity of phytic acid (inositol hexakisphosphate, IHP) has recently become of interest because of the potential for IHP incorporation into red blood cells to achieve a therapeutically useful shift in the hemoglobin-oxygen dissociation curve (Gersonde, K. and Nicolau, C., Blut, 39 (1979) 1). The obser...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(81)90022-6
更新日期：1981-01-01 00:00:00
abstract：:The effects of cadmium on some mitochondrial enzymes of kidney, testis and lung were investigated in male rats. Rats were injected intraperitoneally with 3 mg Cd/kg body weight and its effects were studied 24, 72 and 144 h later. Succinic dehydrogenase and cytochrome c oxidase activities were significantly inhibited i...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(83)90077-x
更新日期：1983-05-01 00:00:00
abstract：:The present study was designed to examine more fully the neurochemical and behavioral interactions that derive from continued lead and cadmium poisoning in pups, whose mothers were exposed via drinking water (300 mg/l of Pb and 10 mg/l of Cd) throughout pregnancy and lactation. At weaning, these metals produced an inc...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(02)00137-3
更新日期：2002-07-01 00:00:00
abstract：:Mitochondrial dysfunction with exposure to anthropogenic contaminants is a critical concern in biomedical and ecological health. Importantly, understanding the role of mitotoxicants in modulating organismal fitness and survival, particularly in the context of species' resilience to shifts in abiotic factors with clima...
journal_title：Toxicology
pub_type： 杂志文章,评审
doi：10.1016/j.tox.2017.07.015
更新日期：2017-11-01 00:00:00
abstract：:The effect of tert-butyl hydroperoxide (tBH) on the nucleotide level in isolated cardiomyocytes from adult rats was examined. Incubation of myocytes with the hydroperoxide resulted in loss of both ATP and NAD from mitochondria and cytosol. In contrast, only the cytosolic level of NADP was increased. The effect on myoc...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(95)03158-c
更新日期：1996-01-08 00:00:00
abstract：:Organochalcogens are important intermediates and useful reagents in organic synthesis, which can increase human exposure risk to these chemicals in the workplace. As well, there are a number of reported cases of acute toxicity following organochalcogen ingestion of vitamins and dietary supplements. Since, the erythroc...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(03)00250-6
更新日期：2003-09-30 00:00:00
abstract：:Gambogic acid (GA), a major active component of gamboge, exhibits potent anticancer activity in many kinds of cancer cells. However, the anticancer mechanism of GA is not clearly understood. Here we showed that GA could cause growth inhibition, induce the G0/G1 phase cell cycle arrest and apoptosis in human chronic my...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2009.04.059
更新日期：2009-08-03 00:00:00
abstract：:Daunomycin-induced cardiotoxicity has been regarded to be the result of oxygen-mediated lipid peroxidation of cell membranes. The aim of the present work was to evaluate the extent of lipid peroxidation in rat heart after administration of this anticancer drug and, further, to examine possible activation of some endog...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/s0300-483x(98)00002-x
更新日期：1998-03-13 00:00:00
abstract：:To investigate mechanisms of chemical-induced congenital heart defects (CHD) we have developed a rat model using dimethadione (DMO), the N-demethylated metabolite of the anticonvulsant, trimethadione (TMD). Dosing pregnant rats with 300mg/kg DMO every 12h from the evening of gestational day (GD) 8 until the morning of...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/j.tox.2014.10.014
更新日期：2014-12-04 00:00:00
abstract：:Cell death triggers the release into extracellular spaces of products of chromatin catabolism, particularly of DNA. A sensitive DNA assay has been used to investigate in the mouse whether the quantitation of plasma DNA may be used as an index of in vivo cytotoxicity. It has been found that toxic doses of bacterial lip...
journal_title：Toxicology
pub_type： 杂志文章
doi：10.1016/0300-483x(90)90178-j
更新日期：1990-04-30 00:00:00
