Hydroperoxide-induced nucleotide degradation and adenosine formation in isolated rat cardiomyocytes.


:The effect of tert-butyl hydroperoxide (tBH) on the nucleotide level in isolated cardiomyocytes from adult rats was examined. Incubation of myocytes with the hydroperoxide resulted in loss of both ATP and NAD from mitochondria and cytosol. In contrast, only the cytosolic level of NADP was increased. The effect on myocyte content of ADP and AMP was less pronounced. A significant increase in formation and release of adenosine also occurred in tBH-exposed cells. Treatment of cardiomyocytes with tBH resulted in a progressive decline of rod-shaped and viable cells, which was accompanied by an increase in numbers of hypercontracted and round myocytes in the incubation mixture. Electron microscopic examination showed an intense contraction of the cells. In summary our results demonstrate that plasma membrane damage and hypercontraction are early manifestations of tBH-induced injury to isolated cardiomyocytes. The depletion of ATP and formation of adenosine also suggest that degradation of adenine nucleotides may contribute to the cytotoxicity caused by tBH. Possible mechanisms involved in the ATP depletion are discussed.






Andersson BS,Vidal RF,Sundberg M,Rajs J,Sótonyi P




Has Abstract


1996-01-08 00:00:00














  • Metabolic pathways of 4-bromo-2,5-dimethoxyphenethylamine (2C-B): analysis of phase I metabolism with hepatocytes of six species including human.

    abstract::4-Bromo-2,5-dimethoxyphenethylamine (2C-B) is a psychoactive designer drug of abuse that is sold under the street names "Venus", "Bromo", "Erox", "XTC" or "Nexus". Concern has been raised because only little is known about its toxicity and metabolism in humans. In the present study we incubated 2C-B with human, monkey...


    pub_type: 杂志文章


    authors: Carmo H,Hengstler JG,de Boer D,Ringel M,Remião F,Carvalho F,Fernandes E,dos Reys LA,Oesch F,de Lourdes Bastos M

    更新日期:2005-01-05 00:00:00

  • Increased levels of hepatic metallothionein in rat and mouse after injection of acetaminophen.

    abstract::Induction of hepatic metallothionein (MT) by acetaminophen was characterized in the rat and mouse. Treatment of rats with the hepatotoxin resulted in increase of liver MT in a dose-dependent manner. MT concentration was elevated by 41%, 140% and 260% following acetaminophen injection at doses of 250, 500 and 1000 mg/k...


    pub_type: 杂志文章


    authors: Wormser U,Calp D

    更新日期:1988-12-30 00:00:00

  • Biological monitoring of butadiene exposure by measurement of haemoglobin adducts.

    abstract::The measurement of haemoglobin (Hb) adducts is an important tool for monitoring exposures to industrial chemicals such as ethylene, ethylene oxide and propylene oxide. The aim of the present study was to use this method to monitor occupational exposure to butadiene. The methodology was evaluated with Hb samples obtain...


    pub_type: 杂志文章


    authors: Richardson KA,Megens HJ,Webb JD,van Sittert NJ

    更新日期:1996-10-28 00:00:00

  • Zebrafish teratogenicity test with metabolic activation (mDarT): effects of phase I activation of acetaminophen on zebrafish Danio rerio embryos.

    abstract::The zebrafish Danio rerio embryo test with metabolic activation (mDarT) was developed to assess the teratogenic effects of proteratogens. In this study induced rat liver microsomes (RLM) were used as a mammalian metabolic activation system (MAS), since they contain various cytochrome P450 (CYP) isoforms at high concen...


    pub_type: 杂志文章


    authors: Weigt S,Huebler N,Braunbeck T,von Landenberg F,Broschard TH

    更新日期:2010-09-10 00:00:00

  • Internet resources for occupational and environmental health professionals.

    abstract::The Internet = s global reach offers new powerful tools to professionals in Occupational and Environmental Health (OEH). The World Wide Web includes extensive free and commercially available reference materials on toxicology, regulatory issues, environmental epidemiology and prevention programs. Much of this especiall...


    pub_type: 杂志文章,评审


    authors: Greenberg GN

    更新日期:2002-04-25 00:00:00

  • Selenium and lead: mutual detoxifying effects.

    abstract::Antagonistic toxic effects of selenium and lead were studied in growing rats. Chronic lead intoxication was produced by cutaneous application of lead naphthenate solution (80-200 mg Pb/kg body weight) for a period of 8 weeks and chronic selenium intoxication was induced by giving 5 ppm, 10 ppm and 15 ppm selenium in d...


    pub_type: 杂志文章


    authors: Rastogi SC,Clausen J,Srivastava KC

    更新日期:1976-11-01 00:00:00

  • Toxic effects of the antifertility agent gossypol in male rats.

    abstract::In the present investigation the influence of gossypol on the male sex function and toxicology in the rat have been studied. Gossypol was injected daily for 5 weeks to adult male rats of the Sprague-Dawley strain with 1 or 10 mg/kg body wt. The low dose of gossypol (1 mg/kg body wt.) did not have any effect on the fol...


    pub_type: 杂志文章


    authors: Gåfvels M,Wang J,Bergh A,Damber JE,Selstam G

    更新日期:1984-09-28 00:00:00

  • Effects of repeated ozone exposure on pulmonary function and bronchial responsiveness in mice sensitized with ovalbumin.

    abstract::We examined the effect of repeated ozone exposure on the respiratory system in mice, sensitized with ovalbumin (OA). OA-sensitized mice and saline-treated control mice were exposed to 1.0 ppm ozone or clean air for 6 h daily and five days weekly for five weeks. Subsequently, these mice were exposed to 1.0 ppm ozone fo...


    pub_type: 杂志文章


    authors: Funabashi H,Shima M,Kuwaki T,Hiroshima K,Kuriyama T

    更新日期:2004-11-01 00:00:00

  • Chromium(III) complexes inhibit transcription factors binding to DNA and associated gene expression.

    abstract::Role of chromium(III) in biological systems is controversial and no significant conclusions can be arrived with the available data. Chromium(VI) enters in to the cell readily and gets reduced to form stable chromium(III) complexes. Chromium(III) complexes react slowly and were considered non-toxic. Recent studies sugg...


    pub_type: 杂志文章


    authors: Raja NS,Nair BU

    更新日期:2008-09-29 00:00:00

  • Immunotoxicologic studies with CI-959, a novel benzothiophene cell activation inhibitor.

    abstract::CI-959 is an orally effective inhibitor of cellular activation in both in vitro and animal models. To assess the effects of CI-959 on immune function, male Fischer 344 rats were evaluated for splenic T- and B-lymphocyte populations, antibody-forming cell response to sheep red blood cells (sRBC), concanavalin A and pok...


    pub_type: 杂志文章


    authors: Bleavins MR,de la Iglesia FA,McCay JA,White KL Jr,Munson AE

    更新日期:1995-04-12 00:00:00

  • Different levels of changes induced by the insecticide lindane in cultured C-6 glioma cells.

    abstract::The effects of lindane on alpha-aminoisobutyric acid and uridine uptake were studied in the rat line C-6 of glial origin. Different levels of modifications could be distinguished by use of 2 modes of investigation. One study, carried out within minutes of contact with lindane, has shown that the inhibition of alpha-am...


    pub_type: 杂志文章


    authors: Roux F,Treich I,Fournier E

    更新日期:1980-01-01 00:00:00

  • Acute toxicity of methyl isocyanate, administered subcutaneously in rabbits: changes in physiological, clinico-chemical and histological parameters.

    abstract::Subcutaneous administration of methyl isocyanate (MIC) in 0.5 LD50 and 1 LD50 doses in female rabbits resulted in significant changes in physiological, clinico-chemical and histological parameters. There was a fall in arterial blood pressure and cardioacceleration in both the 0.5 LD50 and 1 LD50 groups, while the resp...


    pub_type: 杂志文章


    authors: Jeevarathinam K,Selvamurthy W,Ray US,Mukhopadhyay S,Thakur L

    更新日期:1988-10-01 00:00:00

  • Oxidative stress and cytotoxicity of 4-(2-thienyl)butyric acid in isolated rat renal proximal tubular and distal tubular cells.

    abstract::Relative cytotoxicity and mechanism of action of thiophenes were investigated in suspensions of freshly isolated renal proximal tubular (PT) and distal tubular (DT) cells using 4-(2-thienyl)butyric acid (TByA) as a model compound. TByA produced significantly greater time- and concentration-dependent cytotoxicity, as a...


    pub_type: 杂志文章


    authors: Lash LH,Tokarz JJ

    更新日期:1995-12-10 00:00:00

  • Deltamethrin-induced oxidative damage and biochemical alterations in rat and its attenuation by Vitamin E.

    abstract::Deltamethrin is a synthetic pyrethroid insecticide used worldwide in agriculture, home pest control, protection of foodstuff and disease vector control. The objective of this study was to investigate the propensity of deltamethrin to induce oxidative stress and changes in biochemical parameters and enzyme activities i...


    pub_type: 杂志文章


    authors: Yousef MI,Awad TI,Mohamed EH

    更新日期:2006-10-29 00:00:00

  • UV-induced skin damage.

    abstract::Solar radiation induces acute and chronic reactions in human and animal skin. Chronic repeated exposures are the primary cause of benign and malignant skin tumors, including malignant melanoma. Among types of solar radiation, ultraviolet B (290-320 nm) radiation is highly mutagenic and carcinogenic in animal experimen...


    pub_type: 杂志文章,评审


    authors: Ichihashi M,Ueda M,Budiyanto A,Bito T,Oka M,Fukunaga M,Tsuru K,Horikawa T

    更新日期:2003-07-15 00:00:00

  • Does methamphetamine affect bone metabolism?

    abstract::There is a close relationship between the central nervous system activity and bone metabolism. Therefore, methamphetamine (METH), which stimulates the central nervous system, is expected to affect bone turnover. The aim of this study was to investigate the role of METH in bone metabolism. Mice were divided into 3 grou...


    pub_type: 杂志文章


    authors: Tomita M,Katsuyama H,Watanabe Y,Okuyama T,Fushimi S,Ishikawa T,Nata M,Miyamoto O

    更新日期:2014-05-07 00:00:00

  • In vivo hydroquinone exposure impairs allergic lung inflammation in rats.

    abstract::Hydroquinone (HQ) is naturally found in the diet, drugs, as an environmental contaminant and endogenously generated after benzene exposure. Considering that HQ alters the immune system and its several source of exposures in the environment, we hypothesized that prolonged exposure of HQ could affect the course of an im...


    pub_type: 杂志文章


    authors: Macedo SM,Vaz SC,Lourenço EL,de Sousa Mda G,Ligeiro-Oliveira AP,Ferreira JM Jr,Almeida SR,de Lima WT,Farsky SH

    更新日期:2007-11-20 00:00:00

  • Buthionine sulfoximine (BSO) and N-(3,5-dichlorophenyl)succinimide nephrotoxicity: temporal aspects of BSO administration and BSO effects on renal transport systems.

    abstract::The agricultural fungicide, N-(3,5-dichlorophenyl)succinimide (NDPS) induces acute polyuric renal failure which is attenuated by pretreatment with the glutathione depletors, diethyl maleate or buthionine sulfoximine (BSO). In the present study, the temporal aspects of BSO attenuation of NDPS nephrotoxicity were invest...


    pub_type: 杂志文章


    authors: Rankin GO,Beers KW,Teets VJ,Nicoll DW,Anestis DK

    更新日期:1997-02-28 00:00:00

  • Mechanism of the neurotoxicity of 1-methyl-4-phenylpyridinium (MPP+), the toxic bioactivation product of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).

    abstract::It is widely believed that the nigrostriatal toxicity of MPTP is due to its oxidation by brain monoamine oxidase first to MPDP+, and eventually to MPP+. Following uptake by the synaptic dopamine reuptake system, it is concentrated in the matrix of striatal mitochondria by an energy-dependent carrier, energized by the ...


    pub_type: 杂志文章,评审


    authors: Singer TP,Ramsay RR,McKeown K,Trevor A,Castagnoli NE Jr

    更新日期:1988-04-01 00:00:00

  • A rat nasal epithelial model for predicting upper respiratory tract toxicity: in vivo-in vitro correlations.

    abstract::An in vitro model of the rat nasal cavity has been used to compare the responses of nasal tissues in vitro, using loss of intracellular ATP and potassium as indices of toxicity, with the pathological changes occurring following in vivo exposure to four test compounds. Turbinates were incubated in vitro with the test c...


    pub_type: 杂志文章


    authors: Kilgour JD,Simpson SA,Alexander DJ,Reed CJ

    更新日期:2000-04-07 00:00:00

  • Liquid chromatography/electrospray ionization tandem mass spectroscopy (LC/ESI MS/MS) analysis of 1,2-epoxybutene adducts of purine deoxynucleosides.

    abstract::Calf thymus DNA was reacted with 1,2-epoxybutene (BDO) in phosphate buffered saline at 37 degrees C for 12 h. DNA was ethanol precipitated and hydrolyzed with 1 M HCl to release DNA bases. Adenine (A)-BDO and guanine (Gua)-BDO adducts were analyzed in the ethanol supernatant and DNA hydrolysate using LC/ESI MS/MS. Rea...


    pub_type: 杂志文章


    authors: Kambouris SJ,Chaudhary AK,Blair IA

    更新日期:1996-10-28 00:00:00

  • Absorption, distribution, excretion and metabolism of a single oral dose of [14C]tri-o-cresyl phosphate (TOCP) in the male rat.

    abstract::A single oral dose of 50 mg/kg of [14C]TOCP was administered in corn oil to male rats. Three animals were sacrificed at each of 2, 6 and 12 h and 1, 2 and 5 days following dosing, and tissues and excreta were analyzed for 14C. Within 5 days, 63 and 36% of the dose were recovered in the urine and feces, respectively. I...


    pub_type: 杂志文章


    authors: Abou-Donia MB,Nomeir AA,Bower JH,Makkawy HA

    更新日期:1990-12-17 00:00:00

  • Carcinogenicity of 7H-dibenzo[c,g]carbazole, dibenz[a,j]acridine and benzo[a]pyrene in mouse skin and liver following topical application.

    abstract::N-Heterocyclic aromatics are by-products of incomplete combustion of organic material. The overall objective of this study was to determine the relative carcinogenic potencies of 7H-dibenzo[c,g]carbazole (DBC) and dibenz[a,j]acridine (DBA) in a bioassay of complete carcinogenicity on mouse skin in a sensitive strain (...


    pub_type: 杂志文章


    authors: Warshawsky D,Barkley W,Miller ML,LaDow K,Andringa A

    更新日期:1994-11-11 00:00:00

  • Bilirubin enhances neuronal excitability by increasing glutamatergic transmission in the rat lateral superior olive.

    abstract::Hyperbilirubinemia is one of the most common clinical phenomena observed in human newborns. To achieve effective therapeutic treatment, numerous studies have been done to determine the molecular mechanisms of bilirubin-induced neuronal excitotoxicity. However, there is no conclusive evidence for the involvement of glu...


    pub_type: 杂志文章


    authors: Li CY,Shi HB,Song NY,Yin SK

    更新日期:2011-06-18 00:00:00

  • Selective endothelin a (ETA) receptor antagonist (BQ-123) reduces both myocardial infarct size and oxidant injury.

    abstract:OBJECTIVE:Endothelins (ET) can be considered stress-responsive regulators working in paracrine and autocrine fashion. It has been suggested that elevated levels of ET may be responsible for the low coronary re-flow phenomena. Ischemia-reperfusion (I/R) was shown to stimulate ET release in rat heart; however, the mechan...


    pub_type: 杂志文章


    authors: Ozdemir R,Parlakpinar H,Polat A,Colak C,Ermis N,Acet A

    更新日期:2006-02-15 00:00:00

  • Differential effects of D- and L-isomers of triiodothyronine on pituitary TSH secretion and peripheral deiodinase activity in the rat.

    abstract::The effects of D- and L-T3 were compared in male SK & F Wistar rats to define overall effects on the 'pituitary-thyroid-liver axis' at high doses. After in vivo administration of L-T3 (up to 1 mg/kg orally, or up to 0.1 mg/kg subcutaneously) serum TSH and T4 were decreased in a dose-related manner. Similarly, followin...


    pub_type: 杂志文章


    authors: Jones CA,Brown CG,Dickens TA,Atterwill CK

    更新日期:1988-03-01 00:00:00

  • Carcinogenesis by N-nitrosohexamethyleneimine in NZO inbred mice.

    abstract::The carcinogenic response of NZO/BlGd inbred mice of both sexes to oral N-nitrosohexamethyleneimine (NHEX) was investigated at 2 differing dose rates, but equal cumulative doses. Mice received 52 mg NHEX (2 mg/g body wt) in drinking water over either an 8-week period (200 mg/l) or a 32-week period (50 mg/l), and were ...


    pub_type: 杂志文章


    authors: Goodall CM,Lijinsky W

    更新日期:1984-12-01 00:00:00

  • Bone metabolism and oxidative stress in postmenopausal rats with iron overload.

    abstract::Osteoporosis is associated with many etiological causes such as nutrition, cytokines, hormones, and aging. Recently, reactive oxygen species (ROS) are considered to be responsible for the aging process and osteoporosis. We investigated the relationship between ROS and bone metabolism in young female and postmenopausal...


    pub_type: 杂志文章


    authors: Isomura H,Fujie K,Shibata K,Inoue N,Iizuka T,Takebe G,Takahashi K,Nishihira J,Izumi H,Sakamoto W

    更新日期:2004-04-15 00:00:00

  • The intracellular redox stress caused by hexavalent chromium is selective for proteins that have key roles in cell survival and thiol redox control.

    abstract::Hexavalent chromium [Cr(VI)] compounds (e.g. chromates) are strong oxidants that readily enter cells where they are reduced to reactive Cr intermediates that can directly oxidize some cell components and can promote the generation of reactive oxygen and nitrogen species. Inhalation is a major route of exposure which d...


    pub_type: 杂志文章


    authors: Myers JM,Antholine WE,Myers CR

    更新日期:2011-03-15 00:00:00

  • Interaction of air pollutants and pulmonary allergic responses in experimental animals.

    abstract::Asthma, which is primarily an allergic type of respiratory disease, has increased in the U.S. and Europe by 30% over the last decade. Air pollution may play a role in this rise, since during episodes of smog, hospital admissions due to asthma increase. Ambient air quality has generally improved since the Clean Air Act...


    pub_type: 杂志文章,评审


    authors: Gilmour MI

    更新日期:1995-12-28 00:00:00