Abstract:
:Studies showed that certain cytotoxicity assays were not suitable for assessing nanoparticle (NP) toxicity. We evaluated a lactate dehydrogenase (LDH) assay for assessing copper (Cu-40, 40nm), silver (Ag-35, 35nm; Ag-40, 40nm), and titanium dioxide (TiO(2)-25, 25nm) NPs by examining their potential to inactivate LDH and interference with β-nicotinamide adenine dinucleotide (NADH), a substrate for the assay. We also performed a dissolution assay for some of the NPs. We found that the copper NPs, because of their high dissolution rate, could interfere with the LDH assay by inactivating LDH. Ag-35 could also inactivate LDH probably because of the carbon matrix used to cage the particles during synthesis. TiO(2)-25 NPs were found to adsorb LDH molecules. In conclusion, NP interference with the LDH assay depends on the type of NPs and the suitability of the assay for assessing NP toxicity should be examined case by case.
journal_name
Toxicologyjournal_title
Toxicologyauthors
Han X,Gelein R,Corson N,Wade-Mercer P,Jiang J,Biswas P,Finkelstein JN,Elder A,Oberdörster Gdoi
10.1016/j.tox.2011.06.011subject
Has Abstractpub_date
2011-09-05 00:00:00pages
99-104issue
1-3eissn
0300-483Xissn
1879-3185pii
S0300-483X(11)00237-Xjournal_volume
287pub_type
杂志文章相关文献
TOXICOLOGY文献大全abstract::Aerolysin is a cytolytic toxin which forms channels in the plasma membranes of eucaryotic cells. The protein is secreted by Aeromonas hydrophila as an inactive protoxin. Its stability and water solubility are conferred by its ability to dimerize. Maturation of the protein occurs through proteolytic removal of a C-term...
journal_title:Toxicology
pub_type: 杂志文章,评审
doi:10.1016/0300-483x(94)90152-x
更新日期:1994-02-28 00:00:00
abstract::The neurotoxicity and developmental effects of a widely applied insecticide (methomyl) was investigated by a multi-level approach (behavior and biometry, biochemical alterations and neurodegeneration) in Caenorhabditis elegans upon a short-term exposure (1 h) and a post-exposure period (48 h). The 1-h exposure to sub-...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2021.152684
更新日期:2021-01-25 00:00:00
abstract::The agricultural fungicide N-(3,5-dichlorophenyl)succinimide (NDPS) is an acute nephrotoxicant in rats. Our previous studies have strongly suggested that glucuronide conjugation of NDPS metabolites might be a bioactivation step mediating NDPS nephrotoxicity. In this study, effects of substrates and/or inhibitors of pr...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(98)00140-1
更新日期:1999-01-01 00:00:00
abstract::An embryotoxicity study on Ponceau 4 R was carried out in SPF Wistar rats. The substance was given by gavage from day 1 to 20 of the gestation period in doses of 0, 1000, 2000, and 4000 mg/kg bw per day, dissolved in distilled water. The foetuses were removed on day 21. No effect due to the treatment with the dye was ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(75)90117-1
更新日期:1975-11-01 00:00:00
abstract::The distribution and excretion of radioactivity from [14C]citrinin (3 mg/kg, i.v) was determined in male rats. At 0.5 h after administration maximum values of 14.7% and 5.6% of total radioactivity were observed in the liver and kidneys, respectively, and by 6 h decreased to 7.5% in the liver and 4.7% in the kidney. Pl...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(79)90075-1
更新日期:1979-03-01 00:00:00
abstract::Aromatase enzyme plays a central role in steroidogenesis by converting androgens to estrogens and has been proposed as an important molecular target for many environmental endocrine disrupters chemicals. In this study, we have screened 30 selected pesticides with known, unknown or supposed effects on aromatase activit...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.08.021
更新日期:2006-11-10 00:00:00
abstract::In 1975, in cooperation with 8 County Hygienic Stations, total mercury content in fish from rivers and lakes in Hungary was measured. The mercury content in fish muscle-tissue from 164 fish samples averaged 0.36 mg/kg. Average levels in different fish species ranged from 0.10 to 0.57 mg/kg. The amount of mercury in fi...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(77)90052-x
更新日期:1977-06-01 00:00:00
abstract::Daunomycin-induced cardiotoxicity has been regarded to be the result of oxygen-mediated lipid peroxidation of cell membranes. The aim of the present work was to evaluate the extent of lipid peroxidation in rat heart after administration of this anticancer drug and, further, to examine possible activation of some endog...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(98)00002-x
更新日期:1998-03-13 00:00:00
abstract::The Salmonella mutagenicity assay was utilized to compare hepatic S9 fractions derived from wild and laboratory reared woodchucks (Marmota monax). Two promutagens, 7,12-dimethylbenz[a]anthracence (DMBA) and 2-amino-fluorene (AF) were tested at 5 concentrations with the tester strains TA98 and TA100, against 2 levels o...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(88)90058-3
更新日期:1988-01-01 00:00:00
abstract::When Xenopus laevis embryos were treated with fenitrothion, a substantial proportion failed to survive to hatching only when the concentration approached saturation. However, after exposure to 10 ppm of fenitrothion, most were abnormal and did not survive past feeding stage. Different embryonic stages were not equally...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(81)90026-3
更新日期:1981-01-01 00:00:00
abstract::It is well known that selenium is highly toxic to several species of mammals. Here we report the potential neurotoxicity of diselenides, as measured by the manifestation of seizures. The modulation of various neurotransmitter systems potentially involved in seizure episodes and death was also evaluated. The results of...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(02)00423-7
更新日期:2003-02-01 00:00:00
abstract::We have previously shown that young guinea pigs repeatedly exposed to sidestream cigarette smoke (SS) develop decreased airway reactivity of the C-fiber system without changing reactivity to one of its neurotransmitters, substance P (SP). This study was designed to determine whether the decreased reactivity was due to...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(96)03403-8
更新日期:1996-09-02 00:00:00
abstract::It is well-known that organotellurium compounds can have antioxidant activity in vitro, but in vivo these compounds can be potentially toxic to rodents. Here we investigated the potential in vitro and ex vivo toxicity of a new beta-organochalcogenyl vinylphosphonate, the diethyl 2-phenyl-2 tellurophenyl vinylphosphona...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2006.04.027
更新日期:2006-07-05 00:00:00
abstract::The objective of this study was to further evaluate the developmental toxicity of tributyltin chloride (TBTCl) in rats. Pregnant rats were given TBTCl by gastric intubation at a dose of 25, 50 or 100 mg/kg on days 7-9, days 10-12 or days 13-15 of pregnancy. A significant increase in the incidence of post-implantation ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(94)02920-p
更新日期:1995-02-27 00:00:00
abstract::Ferrets were maintained for 12 months on different diets (A, meat and biscuit; B, all meat; C, meat and fish; D, high fibre) to ascertain the cause of spontaneous development of fatty liver. High hepatic triglyceride contents resulted on diets B = C > D; whereas ferrets on diet A (control) showed no accumulation of li...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(96)03351-3
更新日期:1996-08-16 00:00:00
abstract::Bisphenol A (BPA), one of the most prevalent chemicals for daily use, has been reported as a xenoestrogen to induce reproductive toxicity, but its mechanism is poorly understood. In the present study, we aimed to explore whether CaM-CaMKII-ERK1/2 signaling pathway was involved in BPA-induced Sertoli cells injury via t...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2014.06.001
更新日期:2014-10-03 00:00:00
abstract::The development of poisons information centers has in general complied with the actual needs and conditions in the society, and a pragmatic approach and limited funds have been characteristic for these units. To operate in a satisfying way a poisons information center is dependent on two cornerstones-a well educated a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2004.01.033
更新日期:2004-05-20 00:00:00
abstract::The aim of this study is to explore the potent neuroprotective effect of calpeptin (CP) on neuron damage induced by acrylamide (ACR) and its mechanism. Behavioural indicators such as hind limb splay, rota-rod performance, and gait analysis were assessed weekly to evaluate neurobehavioural changes after ACR and/or CP a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2018.03.003
更新日期:2018-05-01 00:00:00
abstract::4-Amino-2,6-dichlorophenol (ADCP) is a potent acute nephrotoxicant in vivo inducing prominent renal corticomedullary necrosis. In vitro, ADCP exposure increases lactate dehydrogenase (LDH) release from rat renal cortical slices at 0.05 mM or greater. The purpose of this study was to examine the ability of antioxidants...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2007.12.014
更新日期:2008-03-12 00:00:00
abstract::Paracetamol, in toxic doses, is associated with extensive liver damage. This represents one of the common causes of morbidity and mortality in drug poisoning cases. This study was undertaken to investigate the possible potentiation of the hepatoprotective action of N-acetylcysteine (NAC) by cimetidine (CMD), an inhibi...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(97)00069-3
更新日期:1997-09-05 00:00:00
abstract::Drug-induced liver injury (DILI) is a major safety concern in drug development and clinical drug therapy. The pathogenesis of DILI usually involves the participation of the parent drug or metabolites that either directly affect the cell biochemistry or elicit an immune response. However, in most cases the mechanisms a...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2010.05.011
更新日期:2010-09-10 00:00:00
abstract::Valproic acid (VPA) and the unsaturated metabolites, 2-ene VPA and (E)-2,(Z)-3'-diene VPA, demonstrated dose-dependent cytotoxicity in primary cultures of rat hepatocytes, as evaluated by lactate dehydrogenase (LDH) leakage. Cellular glutathione (GSH) was depleted by adding buthionine sulfoximine (BSO) to the culture ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(96)03352-5
更新日期:1996-08-01 00:00:00
abstract::Chemical carcinogens are generally classified as genotoxic or non-genotoxic. However, weak genotoxicity at high concentrations is sometimes observed and interpretation is often problematic. In addition, certain rodent carcinogens exert their effects at doses associated with cytotoxicity and compensatory hyperplasia ma...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(03)00058-1
更新日期:2003-05-03 00:00:00
abstract::A single oral dose of 50 mg/kg of [14C]TOCP was administered in corn oil to male rats. Three animals were sacrificed at each of 2, 6 and 12 h and 1, 2 and 5 days following dosing, and tissues and excreta were analyzed for 14C. Within 5 days, 63 and 36% of the dose were recovered in the urine and feces, respectively. I...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/0300-483x(90)90079-v
更新日期:1990-12-17 00:00:00
abstract::The scandal of melamine-contamination has not been quite blown out, since the toxicity of melamine continues to raise concerns for public health. It has been well known that fetus and infant periods play the most important roles in brain development, whereas little has been done on the harmful effects of melamine on t...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.08.011
更新日期:2011-11-18 00:00:00
abstract::Synthetic pyrethroids are used as insecticides in agriculture and a variety of household applications worldwide. Pyrethroids are widely distributed in all environmental compartments and the general populations are exposed to pyrethroids through various routes. Pyrethroids have been identified as endocrine-disrupting c...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2020.152460
更新日期:2020-05-30 00:00:00
abstract::The current Organisation for Economic Co-operation and Development (OECD) guidelines for evaluating organophosphorus-induced delayed neuropathy (OPIDN) require the observation of dosed animals over several days and the sacrifice of 48 hens. Adhering to these protocols in tests with enantiomers is difficult because lar...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2011.12.004
更新日期:2012-02-26 00:00:00
abstract::We examined the effect of repeated ozone exposure on the respiratory system in mice, sensitized with ovalbumin (OA). OA-sensitized mice and saline-treated control mice were exposed to 1.0 ppm ozone or clean air for 6 h daily and five days weekly for five weeks. Subsequently, these mice were exposed to 1.0 ppm ozone fo...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/j.tox.2004.06.047
更新日期:2004-11-01 00:00:00
abstract::In the present study, we demonstrated the cytotoxic effect of a dimethylarsenic compound in seaweed, (R)-(2',3'-dihydroxypropyl) 5- deoxy-5-dimethylarsinoyl-beta-D-riboside, namely arsenosugar (AsSug), on mammalian cells, murine macrophages, in comparison with that of an inorganic arsenical, arsenite, in vitro. More t...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(97)00101-7
更新日期:1997-10-19 00:00:00
abstract::Toxicity studies with drugs in animals are performed to establish the toxicity profile including the no-toxic-effect-level in the animals. One of the responses used in the search for effects is organ weight. Since organ weight is related to body weight of the animal, the analysis of organ weight has to be adjusted. A ...
journal_title:Toxicology
pub_type: 杂志文章
doi:10.1016/s0300-483x(99)00056-6
更新日期:1999-08-31 00:00:00