Abstract:
:Thymidylate synthase (TS) is an essential enzyme for DNA biosynthesis that is an important target of fluoropyrimidine anti-cancer drugs such as 5-fluorouracil (5-FU), which is frequently used against human colon adenocarcinomas. Because levels of TS in such tumors may be predictive of chemotherapeutic response to 5-FU, it is desirable to have a method for TS quantitation simpler than the laborious conventional biochemical assays that currently exist. We therefore describe a rapid and simple immunoassay for quantitating human TS (in cell or tissue extracts) that employs a rabbit antibody to TS from bacteriophage T4 and an automated colorimetric readout. The sensitivity of the assay is demonstrated by its ability to show a different TS level in cytosols from each of 8 human colorectal carcinoma cell lines tested and a 2.5-fold difference between the highest and lowest values observed. Western immunoblot was used to show that the rabbit antibody to T4 TS cross-reacts very specifically with human TS enzyme. This indicates that some evolutionarily conserved (phage or bacterial) enzymes, not purified from human sources, may be useful as antigens in producing anti-sera for human diagnostic tests.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Weitz S,Wildrick D,Boman Bsubject
Has Abstractpub_date
1993-02-01 00:00:00pages
275-8issue
2eissn
1019-6439issn
1791-2423journal_volume
2pub_type
杂志文章abstract::The aim of the present review was to summarize the current knowledge of the involvement of nestin in breast cancer (BC) pathogenesis. Nestin is a member of the class VI family of intermediate filament proteins, originally identified as a marker of neural stem cells and subsequently demonstrated to be expressed in BC a...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2018.4441
更新日期:2018-08-01 00:00:00
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journal_title:International journal of oncology
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章,评审
doi:
更新日期:2003-03-01 00:00:00
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doi:
更新日期:2005-04-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 临床试验,杂志文章,随机对照试验
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