Abstract：
:The defining characteristic of obesity is increased adipose tissue (AT) mass following chronic positive energy supply. AT mass is determined by adipocyte number and size, which reflect proliferation and differentiation of preadipocytes and hypertrophy of pre-existing adipocytes. The molecular pathways governing AT expansion are incompletely defined. We previously reported that FGF-1 primes proliferating primary human preadipocytes (phPA), thereby increasing adipogenesis. Here we examined whether FGF-1's adipogenic actions were due to modulation of other FGFs. Treatment of phPA with FGF-1 reduced FGF-2 mRNA/protein by 80%. To examine a putative functional role we performed siRNA knockdown studies. Following FGF-2 knockdown preadipocyte proliferation was decreased and expression of adipogenic genes (PPARγ, G3PDH and adiponectin) was increased at day 1 of differentiation. These results suggest that changes in endogenous FGF-2 levels contribute to FGF-1's early adipogenic effects and highlight the complexity of the paracrine interplay between FGFs within human AT.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Hutley LJ,Newell FS,Kim YH,Luo X,Widberg CH,Shurety W,Prins JB,Whitehead JPdoi
10.1016/j.mce.2011.04.012subject
Has Abstractpub_date
2011-06-06 00:00:00pages
165-71issue
1-2eissn
0303-7207issn
1872-8057pii
S0303-7207(11)00210-3journal_volume
339pub_type
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