Abstract:
:Synthetic soluble Aβ oligomers are often used as a surrogate for biologic material in a number of model systems. We compared the activity of Aβ oligomers (synthetic and cell culture media derived) on the human SH-SY5Y neuroblastoma and C2C12 mouse myoblast cell lines in a novel, modified MTT assay. Separating oligomers from monomeric peptide by size exclusion chromatography produced effects at peptide concentrations approaching physiologic levels (10-100 nM). Purified oligomers, but not monomers or fibrils, elicited an increase of a detergent-insoluble form of MTT formazan within 2h as opposed to a control toxin (H(2)O(2)). This effect was comparable for biological and synthetic peptide in both cell types. Monomeric Aβ attenuated the effect of soluble oligomers. This study suggests that the activities of biological and synthetic oligomers are indistinguishable during early stages of Aβ oligomer-cell interaction.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Weidner AM,Housley M,Murphy MP,Levine H 3rddoi
10.1016/j.neulet.2011.03.082subject
Has Abstractpub_date
2011-06-15 00:00:00pages
1-5issue
1eissn
0304-3940issn
1872-7972pii
S0304-3940(11)00406-Xjournal_volume
497pub_type
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