Abstract:
:The function of neuronal networks relies on selective assembly of synaptic connections during development. We examined how synaptic specificity emerges in the pontocerebellar projection. Analysis of axon-target interactions with correlated light-electron microscopy revealed that developing pontine mossy fibers elaborate extensive cell-cell contacts and synaptic connections with Purkinje cells, an inappropriate target. Subsequently, mossy fiber-Purkinje cell connections are eliminated resulting in granule cell-specific mossy fiber connectivity as observed in mature cerebellar circuits. Formation of mossy fiber-Purkinje cell contacts is negatively regulated by Purkinje cell-derived BMP4. BMP4 limits mossy fiber growth in vitro and Purkinje cell-specific ablation of BMP4 in mice results in exuberant mossy fiber-Purkinje cell interactions. These findings demonstrate that synaptic specificity in the pontocerebellar projection is achieved through a stepwise mechanism that entails transient innervation of Purkinje cells, followed by synapse elimination. Moreover, this work establishes BMP4 as a retrograde signal that regulates the axon-target interactions during development.
journal_name
PLoS Bioljournal_title
PLoS biologyauthors
Kalinovsky A,Boukhtouche F,Blazeski R,Bornmann C,Suzuki N,Mason CA,Scheiffele Pdoi
10.1371/journal.pbio.1001013subject
Has Abstractpub_date
2011-02-08 00:00:00pages
e1001013issue
2eissn
1544-9173issn
1545-7885journal_volume
9pub_type
杂志文章相关文献
PLOS BIOLOGY文献大全abstract::During in vitro fertilisation (IVF), pharmacological activation of the murine X chromosome-encoded receptor proteins Toll-like receptor (TLR) 7 and TLR8 reportedly results in male-biased litters by selectively disrupting the motility of X-bearing sperm cells. Thus-in the context of agonist treatment during IVF-these r...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000663
更新日期:2020-03-23 00:00:00
abstract::DEAD-box proteins are ubiquitous regulators of RNA biology. While commonly dubbed "helicases," their activities also include duplex annealing, adenosine triphosphate (ATP)-dependent RNA binding, and RNA-protein complex remodeling. Rok1, an essential DEAD-box protein, and its cofactor Rrp5 are required for ribosome ass...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002480
更新日期:2016-06-09 00:00:00
abstract::The link between single-cell variation and population-level fate choices lacks a mechanistic explanation despite extensive observations of gene expression and epigenetic variation among individual cells. Here, we found that single human embryonic stem cells (hESCs) have different and biased differentiation potentials ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000453
更新日期:2019-09-26 00:00:00
abstract::In eukaryotes, the highly conserved U3 small nucleolar RNA (snoRNA) base-pairs to multiple sites in the pre-ribosomal RNA (pre-rRNA) to promote early cleavage and folding events. Binding of the U3 box A region to the pre-rRNA is mutually exclusive with folding of the central pseudoknot (CPK), a universally conserved r...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002083
更新日期:2015-02-24 00:00:00
abstract::Centrosomes comprise a pair of centrioles surrounded by an amorphous pericentriolar material (PCM). Here, we have performed a microscopy-based genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins required for centriole duplication and mitotic PCM recruitment. We analysed 92% of the Droso...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0060224
更新日期:2008-09-16 00:00:00
abstract::Intestinal epithelial cells exist within a complex environment that affects how they interpret and respond to stimuli. We have applied a multi-scale in vivo systems approach to understand how intestinal immune cells communicate with epithelial cells to regulate responses to inflammatory signals. Multivariate modeling ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001393
更新日期:2012-01-01 00:00:00
abstract::Morphogen gradients are established by the localized production and subsequent diffusion of signaling molecules. It is generally assumed that cell fates are induced only after morphogen profiles have reached their steady state. Yet, patterning processes during early development occur rapidly, and tissue patterning may...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0050046
更新日期:2007-02-01 00:00:00
abstract::The gene GAD2 encoding the glutamic acid decarboxylase enzyme (GAD65) is a positional candidate gene for obesity on Chromosome 10p11-12, a susceptibility locus for morbid obesity in four independent ethnic populations. GAD65 catalyzes the formation of gamma-aminobutyric acid (GABA), which interacts with neuropeptide Y...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0000068
更新日期:2003-12-01 00:00:00
abstract::Using DNA sequences 5' to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expre...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0050237
更新日期:2007-09-01 00:00:00
abstract::Creating artificial protein families affords new opportunities to explore the determinants of structure and biological function free from many of the constraints of natural selection. We have created an artificial family comprising 3,000 P450 heme proteins that correctly fold and incorporate a heme cofactor by recombi...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0040112
更新日期:2006-05-01 00:00:00
abstract::The mechanism by which a complex auditory scene is parsed into coherent objects depends on poorly understood interactions between task-driven and stimulus-driven attentional processes. We illuminate these interactions in a simultaneous behavioral-neurophysiological study in which we manipulate participants' attention ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1000129
更新日期:2009-06-01 00:00:00
abstract::Our current understanding of biology is heavily based on a small number of genetically tractable model organisms. Most eukaryotic phyla lack such experimental models, and this limits our ability to explore the molecular mechanisms that ultimately define their biology, ecology, and diversity. In particular, marine prot...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2006333
更新日期:2018-07-02 00:00:00
abstract::Previously it was shown that male mice, when they encounter female mice or their pheromones, emit ultrasonic vocalizations with frequencies ranging over 30-110 kHz. Here, we show that these vocalizations have the characteristics of song, consisting of several different syllable types, whose temporal sequencing include...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0030386
更新日期:2005-12-01 00:00:00
abstract::Hosts are likely to respond to parasitic infections by a combination of resistance (expulsion of pathogens) and tolerance (active mitigation of pathology). Of these strategies, the basis of tolerance in animal hosts is relatively poorly understood, with especially little known about how tolerance is manifested in natu...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001901
更新日期:2014-07-08 00:00:00
abstract::Morphological development of fungi and their combined production of secondary metabolites are both acting in defence and protection. These processes are mainly coordinated by velvet regulators, which contain a yet functionally and structurally uncharacterized velvet domain. Here we demonstrate that the velvet domain o...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001750
更新日期:2013-12-01 00:00:00
abstract::The most common form of human autosomal dominant hereditary spastic paraplegia (AD-HSP) is caused by mutations in the SPG4 (spastin) gene, which encodes an AAA ATPase closely related in sequence to the microtubule-severing protein Katanin. Patients with AD-HSP exhibit degeneration of the distal regions of the longest ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0020429
更新日期:2004-12-01 00:00:00
abstract::Metastatic dissemination employs both the blood and lymphatic vascular systems. Solid tumors dynamically remodel and generate both vessel types during cancer progression. Lymphatic vessel invasion and cancer cells in the tumor-draining lymph nodes (LNs) are prognostic markers for breast cancer metastasis and patient o...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.2005907
更新日期:2018-12-28 00:00:00
abstract::Blood-brain barrier (BBB) defects and cerebrovascular dysfunction contribute to amyloid-β (Aβ) brain accumulation and drive Alzheimer disease (AD) pathology. By regulating vascular functions and inflammation in the microvasculature, a disintegrin and metalloprotease with thrombospondin type I motif, member 13 (ADAMTS1...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000313
更新日期:2019-06-11 00:00:00
abstract::Chronic inflammation is now a well-known precursor for cancer development. Infectious prostatitis are the most common causes of prostate inflammation, but emerging evidence points the role of metabolic disorders as a potential source of cancer-related inflammation. Although the widely used treatment for prostate cance...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000948
更新日期:2020-12-07 00:00:00
abstract::A large repertoire of natural object manipulation tasks require precisely coupled symmetrical opposing forces by both hands on a single object. We asked how the lateralized brain handles this basic problem of spatial and temporal coordination. We show that the brain consistently appoints one of the hands as prime acto...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0040158
更新日期:2006-06-01 00:00:00
abstract::The illusion of apparent motion can be induced when visual stimuli are successively presented at different locations. It has been shown in previous studies that motion-sensitive regions in extrastriate cortex are relevant for the processing of apparent motion, but it is unclear whether primary visual cortex (V1) is al...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0030265
更新日期:2005-08-01 00:00:00
abstract::Cell proliferation and programmed cell death are closely controlled during animal development. Proliferative stimuli generally also induce apoptosis, and anti-apoptotic factors are required to allow net cell proliferation. Genetic studies in Drosophila have led to identification of a number of genes that control both ...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.0000035
更新日期:2003-11-01 00:00:00
abstract::Birds utilize a unique structure, called a syrinx, for the production of their vocalizations. The origins of the syrinx are not well understood. New work, utilizing first principles-based models, suggests that a key element in selection for the early syrinx might be the position of this vocal structure: although the l...
journal_title:PLoS biology
pub_type: 评论,杂志文章
doi:10.1371/journal.pbio.3000184
更新日期:2019-03-28 00:00:00
abstract::The ability to find and consume nutrient-rich diets for successful reproduction and survival is fundamental to animal life. Among the nutrients important for all animals are polyamines, a class of pungent smelling compounds required in numerous cellular and organismic processes. Polyamine deficiency or excess has detr...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1002454
更新日期:2016-05-04 00:00:00
abstract::The human gut harbours a large and genetically diverse population of symbiotic microbes that both feed and protect the host. Evolutionary theory, however, predicts that such genetic diversity can destabilise mutualistic partnerships. How then can the mutualism of the human microbiota be explained? Here we develop an i...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001424
更新日期:2012-01-01 00:00:00
abstract::Phenotypic heterogeneity can confer clonal groups of organisms with new functionality. A paradigmatic example is the bistable expression of virulence genes in Salmonella typhimurium, which leads to phenotypically virulent and phenotypically avirulent subpopulations. The two subpopulations have been shown to divide lab...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.1001928
更新日期:2014-08-19 00:00:00
abstract::Infants' cognitive development and learning rely profoundly on their interactions with other people. In the first year, infants become increasingly sensitive to others' gaze and use it to focus their own attention on relevant visual input. However, infants are not passive observers in early social interactions, and th...
journal_title:PLoS biology
pub_type: 评论,杂志文章
doi:10.1371/journal.pbio.3000055
更新日期:2018-12-13 00:00:00
abstract::Neo-sex chromosomes are found in many taxa, but the forces driving their emergence and spread are poorly understood. The female-specific neo-W chromosome of the African monarch (or queen) butterfly Danaus chrysippus presents an intriguing case study because it is restricted to a single 'contact zone' population, invol...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000610
更新日期:2020-02-27 00:00:00
abstract::What do "microbes" have to do with social equity? These microorganisms are integral to our health, that of our natural environment, and even the "health" of the environments we build. The loss, gain, and retention of microorganisms-their flow between humans and the environment-can greatly impact our health. It is well...
journal_title:PLoS biology
pub_type: 杂志文章
doi:10.1371/journal.pbio.3000536
更新日期:2019-11-26 00:00:00
abstract::Placebo response in the clinical trial setting is poorly understood and alleged to be driven by statistical confounds, and its biological underpinnings are questioned. Here we identified and validated that clinical placebo response is predictable from resting-state functional magnetic-resonance-imaging (fMRI) brain co...
journal_title:PLoS biology
pub_type: 杂志文章,随机对照试验
doi:10.1371/journal.pbio.1002570
更新日期:2016-10-27 00:00:00