Abstract：
BACKGROUND:Aquaporin 5 (AQP5) is widely expressed in various organ and tissues. In light of the novel oncogenic properties of aquaporins (AQPs), here we investigated the effect of AQP5 knockdown by RNAi on transmembrane osmotic water permeability, cell migration potential and cell volume regulation ability. METHODS:AQP5 expression was inhibited by short hairpin RNA in SPC-A1 cells, a lung adenocarcinoma cell line. Cells loaded with a fluoroprobe (calcein-AM) were immersed in either isosmotic, hyperosmotic or hyposmotic solutions, and fluorescence intensity was recorded using confocal microscopy. These measurements were used to calculate osmotic water permeability coefficients (Pf) and to monitor regulated volume decrease (RVD). Tumor cell migration and invasion assays were performed in a modified Boyden chamber. Wound healing and colony forming ability were also tested. RESULTS:Although self-quenching was not found in SPC-A1 cells, we observed a linear relationship between fluorescence intensity and cell water volume, suggesting that this method is a sensitive and reproducible way to measure single-cell transmembrane water permeability. Cells in which the AQP5 gene was silenced showed a 49.4% decrease in osmotic water permeability, a 55.3% decrease in migration and a 28.4% decrease in invasion potential. In addition, RVD decreased remarkably with reduced osmotic water permeability. CONCLUSION:Our results suggest that AQP5, which mediates water permeability and thus regulates cell shape and volume, is a potentially important determinant in cell migration.
journal_name
Respir Physiol Neurobioljournal_title
Respiratory physiology & neurobiologyauthors
Chen Z,Zhang Z,Gu Y,Bai Cdoi
10.1016/j.resp.2011.02.001subject
Has Abstractpub_date
2011-05-31 00:00:00pages
110-7issue
3eissn
1569-9048issn
1878-1519pii
S1569-9048(11)00051-6journal_volume
176pub_type
杂志文章abstract：:The prefrontal cortex is one of the key areas of the central mechanism of cardiovascular and respiratory control. Disinhibition of the prelimbic medial prefrontal cortex elicits tachypnoeic responses in anesthetized rats (Hassan et al., J. Physiol. 591: 6069-6088, 2013). The current study examines the effects of inhib...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2014.07.009
更新日期：2014-12-01 00:00:00
abstract：:Newborn mammals exhibit a biphasic hypoxic ventilatory response (HVR) in which an initial increase in ventilation is followed by a decline back toward baseline levels. The magnitude of the secondary decline diminishes with postnatal age, but this transition occurs earlier in rat pups reared in moderate hyperoxia. This...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2020.103483
更新日期：2020-09-01 00:00:00
abstract：:Infants who are born premature can have persistent apnea beyond term gestation, reemergence of apnea associated with inflammation during infancy, increased risk of sudden unexplained death, and sleep disorder breathing during infancy and childhood. The autonomic nervous system, particularly the central neural networks...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2019.103337
更新日期：2020-03-01 00:00:00
abstract：:In response to the increase in oxygen tension at birth, the resistance pulmonary arteries dilate, while the ductus arteriosus constricts. Although modulated by the endothelium, these opposite responses are intrinsic to the vascular smooth muscle. While still controversial, it seems likely that during normoxia the prod...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/s1569-9048(02)00054-x
更新日期：2002-08-22 00:00:00
abstract：:Recurrent sleep apnea (RSA), mimicking chronic intermittent hypoxia (CIH), may trigger unique adaptations in oxygen sensing in the carotid body, and consequent cellular functions unlike the effects of sustained hypoxia (SH). As a mechanism, an augmented generation of reactive oxygen species (ROS) in CIH has been invok...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/s0034-5687(01)00343-7
更新日期：2002-06-01 00:00:00
abstract：:This study tested the hypothesis that, by increasing the volume available for tidal expansion (inspiratory capacity, IC), bi-level positive airway pressure (BiPAP™) would lead to greater beneficial effects on dyspnea and exercise intolerance in comorbid heart failure (HF)-chronic obstructive pulmonary disease (COPD) t...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2019.04.008
更新日期：2019-08-01 00:00:00
abstract：:Tyrosine kinase inhibitors (TKI), erlotinib and gefitinib are small molecule inhibitors which are used for the treatment of lung cancer. But, the development of drug resistance has been reported as one of the major setbacks in oncology. This study focused on the mechanisms leading to secondary resistance by assessing ...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2014.09.022
更新日期：2015-04-01 00:00:00
abstract：:Inorganic nitrate has been shown to reduce oxygen cost during exercise. Since the nitrate-nitrite-NO pathway is facilitated during hypoxia, we investigated the effects of dietary nitrate on oxygen consumption and cardiovascular responses during apnea. These variables were measured in two randomized, double-blind, plac...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.resp.2012.09.008
更新日期：2013-01-15 00:00:00
abstract：:The relationship among airway responsiveness, inflammation and remodelling in asthma is incompletely understood. To investigate potential mechanistic associations, allergen-induced asthma was studied in C57Bl/6 mice. Mice were sensitized and challenged with ovalbumin (OVA) using sub-acute (SA) or chronic (C) protocols...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2009.08.005
更新日期：2009-10-31 00:00:00
abstract：:To evaluate whether respiratory factors limit exercise capacity in patients with mild cystic fibrosis (CF) lung disease (mean FEV(1) = 76 +/- 7.7% predicted) we stressed the respiratory system of seven patients using added dead space (V(D)). Primary outcomes were exercise duration (Ex(dur)) and maximal oxygen uptake (...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2005.08.003
更新日期：2006-06-01 00:00:00
abstract：:Dyspnea on exertion (DOE) is a common symptom in obesity. We investigated whether aerobic exercise training without weight loss could reduce DOE. Twenty-two otherwise healthy obese women participated in a 12-week supervised aerobic exercise training program, exercising 30 min/day at 70-80% heart rate reserve, 4 days/w...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2015.11.004
更新日期：2016-01-15 00:00:00
abstract：:To assess the occurrence of tidal expiratory flow limitation (EFL) and/or dynamic pulmonary hyperinflation (DH) in chronic heart failure (CHF) during exercise 15 patients with stable systolic CHF, aged 69 ± 6yr, underwent pulmonary function testing and incremental cardio-pulmonary exercise testing. They subsequently p...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2013.06.017
更新日期：2013-10-01 00:00:00
abstract：:A computational fluid dynamics (CFD) modelling approach is used to study the unsteady respiratory airflow dynamics within a human lung. The three-dimensional asymmetric bifurcation model of the central airway based on the morphological data given by Horsfield et al. (J. Appl. Physiol. 67 (1971) 207) was used in the pr...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/s0034-5687(01)00337-1
更新日期：2002-04-01 00:00:00
abstract：:The Na+/H+ exchangers (NHEs) are a family of antiporters involved in the maintenance of neural steady-state intracellular pH. The NHE3 seems to be the predominant subtype in central chemosensitive cells. We aimed to analyze the effect of a selective NHE3 inhibition on the respiratory pattern in spontaneously breathing...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2008.12.011
更新日期：2009-02-28 00:00:00
abstract：:If, as postulated, the end of the steady state phase (φ2) of cardiovascular responses to apnoea corresponds to the physiological breaking point, then we may hypothesize that φ2 should become visible if exercise apnoeas are performed in pure oxygen. We tested this hypothesis on 9 professional divers by means of continu...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2018.05.003
更新日期：2018-09-01 00:00:00
abstract：:We previously found that maternal cigarette smoke (CS) exposure resulted in impairment of central chemoreception and oxidative stress and mitochondrial dysfunction of parafacial respiratory group (pFRG, a critical site for mammalian central chemoreception) in neonatal rats. The present work was carried out to identify...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2020.103383
更新日期：2020-03-01 00:00:00
abstract：:Theoretical data for the alveolar amplitude response technique (AART) (J. Appl. Physiol. 41 (1976) 419-424) for assessing lung function was simulated using a single path lung model. This model takes account of stratified inhomogeneities in gas concentrations within the respiratory tree. The data was inserted into prev...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/s1569-9048(03)00124-1
更新日期：2003-08-14 00:00:00
abstract：BACKGROUND:During the COVID-19 outbreak, a very high number of infected patients developed pneumonia and many of them complicated with acute respiratory distress syndrome. The optimal management of respiratory failure and the role of lung ultrasound imaging in the evaluation of efficacy of treatment are unknown. METHO...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2020.103489
更新日期：2020-09-01 00:00:00
abstract：:The comparatively low compliance of the mammalian lung results in an evolutionary dilemma: the origin and evolution of this bronchoalveolar lung into a high-performance gas-exchange organ results in a high work of breathing that cannot be achieved without the coupled evolution of a muscular diaphragm. However, despite...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2010.01.004
更新日期：2010-04-15 00:00:00
abstract：BACKGROUND:The purpose of this study was to explore the effect of Wnt pathway on the inhibition of airway epithelial cells repair by glucocorticoid. MATERIALS AND METHODS:The expression of E-cadherin in asthma mice model was detected by immunocytochemistry. XAV939 was used to treat 16HBE, and the expressions of relate...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2019.103283
更新日期：2020-01-01 00:00:00
abstract：:Loss of alveolar barrier function is important in the development of pulmonary edema, but quantitation of its integrity has been difficult in the intact lung. We report a new non-radioactive method to assess paracellular and transcellular permeability of alveolar barrier in buffer-perfused rabbit lungs. Changes in alv...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/s1569-9048(02)00150-7
更新日期：2002-10-23 00:00:00
abstract：:The lung surfactant dipalmitoylphosphatidylcholine (DPPC) most probably leaks into the blood, settling on the luminal aspect of blood vessels to create active hydrophobic spots (AHS). Nanobubbles are formed at these spots from dissolved gas. We hypothesized that when a large molecule in the blood comes into contact wi...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2020.103615
更新日期：2021-01-06 00:00:00
abstract：:The present studies evaluate whether the vagus nerves link the lungs' immune and neural systems by transmitting information through pulmonary nociceptors. Single unit activities from pulmonary nociceptors [C fiber receptors (CFRs) and high threshold Adelta fiber receptors (HTARs)] were recorded from the cervical vagus...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2006.11.005
更新日期：2007-05-14 00:00:00
abstract：:Anxiety disorders, particularly panic disorder (PD), are associated with respiratory abnormalities. PD consists of unexpected panic attacks (PA) with anxiety, fear and many autonomic and respiratory symptoms. There is a substantial body of literature demonstrating that stimulation of respiration is a common event in p...
journal_title：Respiratory physiology & neurobiology
pub_type： 社论,评审
doi：10.1016/j.resp.2008.07.011
更新日期：2009-05-30 00:00:00
abstract：:Breathing disturbances are a major challenge in Rett Syndrome (RTT). These disturbances are more pronounced during wakefulness; but irregular breathing occurs also during sleep. During the day patients can exhibit alternating bouts of hypoventilation and irregular hyperventilation. But there is significant individual ...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2013.06.022
更新日期：2013-11-01 00:00:00
abstract：:China had taken strict measures for pandemic 2009 H1N1 infection with enhanced surveillance and hospital isolation since April 2009. In Shenzhen, over 1200 confirmed cases of H1N1 infection were identified. Three young patients died of severe pneumonia. Among them, two boys developed neurological complications. Cytoki...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2010.11.004
更新日期：2011-01-31 00:00:00
abstract：:Preoperative prediction of a successful outcome following lung volume reduction surgery (LVRS) for emphysema is imperfect. One mechanism could be improvement in respiratory muscle function yet controversy exists regarding the magnitude and mechanism of such an improvement. Therefore, we measured diaphragm strength in ...
journal_title：Respiratory physiology & neurobiology
pub_type： 临床试验,杂志文章
doi：10.1016/j.resp.2005.03.010
更新日期：2006-02-28 00:00:00
abstract：:The neuropeptide relaxin-3 is expressed by the pontine nucleus incertus. Relaxin-3 and synthetic agonist peptides modulate arousal and cognitive processes via activation of the relaxin-family peptide 3 receptor (RXFP3). Despite the presence of RXFP3 in the nucleus of the solitary tract (NTS), the ability of RXFP3 to m...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2019.103310
更新日期：2020-01-01 00:00:00
abstract：:Expression of the transcription factor FOXP2 is linked to brain circuits that control motor function and speech. Investigation of FOXP2 protein expression in respiratory areas of the ponto-medullary brainstem of adult rat revealed distinct rostro-caudal expression gradients. A high density of FOXP2 immunoreactive nucl...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2018.01.014
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND:The aim of this study was to determine the relationship between body position, pulmonary function (PF) and bronchial blood flow (Q(aw)) in a group of heart failure (HF) and control subjects. METHODS:Thirty-six subjects were studied: 24 stable, ambulatory HF patients (HF: LVEF=27±6%, age=65±9 yr) and 12 age-...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2011.06.021
更新日期：2011-09-15 00:00:00
