Impaired migration and cell volume regulation in aquaporin 5-deficient SPC-A1 cells.


BACKGROUND:Aquaporin 5 (AQP5) is widely expressed in various organ and tissues. In light of the novel oncogenic properties of aquaporins (AQPs), here we investigated the effect of AQP5 knockdown by RNAi on transmembrane osmotic water permeability, cell migration potential and cell volume regulation ability. METHODS:AQP5 expression was inhibited by short hairpin RNA in SPC-A1 cells, a lung adenocarcinoma cell line. Cells loaded with a fluoroprobe (calcein-AM) were immersed in either isosmotic, hyperosmotic or hyposmotic solutions, and fluorescence intensity was recorded using confocal microscopy. These measurements were used to calculate osmotic water permeability coefficients (Pf) and to monitor regulated volume decrease (RVD). Tumor cell migration and invasion assays were performed in a modified Boyden chamber. Wound healing and colony forming ability were also tested. RESULTS:Although self-quenching was not found in SPC-A1 cells, we observed a linear relationship between fluorescence intensity and cell water volume, suggesting that this method is a sensitive and reproducible way to measure single-cell transmembrane water permeability. Cells in which the AQP5 gene was silenced showed a 49.4% decrease in osmotic water permeability, a 55.3% decrease in migration and a 28.4% decrease in invasion potential. In addition, RVD decreased remarkably with reduced osmotic water permeability. CONCLUSION:Our results suggest that AQP5, which mediates water permeability and thus regulates cell shape and volume, is a potentially important determinant in cell migration.


Chen Z,Zhang Z,Gu Y,Bai C




Has Abstract


2011-05-31 00:00:00














  • Effect of lung volume reduction surgery for emphysema on diaphragm function.

    abstract::Preoperative prediction of a successful outcome following lung volume reduction surgery (LVRS) for emphysema is imperfect. One mechanism could be improvement in respiratory muscle function yet controversy exists regarding the magnitude and mechanism of such an improvement. Therefore, we measured diaphragm strength in ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 临床试验,杂志文章


    authors: Hamnegård CH,Polkey MI,Thylen A,Nilsson F,Schersten H,Bake B

    更新日期:2006-02-28 00:00:00

  • Interaction of tobacco smoke exposure and ovalbumin-sensitization promotes goblet cell and submucosal gland metaplasia in guinea pigs.

    abstract::Exposure to irritants such as tobacco smoke (TS) causes acute airway inflammation. Chronic exposure may cause airway remodeling contributing to enhanced airway resistance. We hypothesize that combining airway sensitization and inhalation of irritants enhances the number of mucous producing cells beyond either agent al...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Pavuluri S,Hanus V,Bergren DR

    更新日期:2013-12-01 00:00:00

  • Effect of chronic heart failure in older rats on respiratory muscle and hindlimb blood flow during submaximal exercise.

    abstract::Submaximal exercise diaphragm blood flow (BF) is elevated in young chronic heart failure (CHF) rats, while it is unknown if this occurs in older animals. Respiratory and hindlimb muscle BFs (radiolabeled microspheres) were measured at rest and during submaximal exercise (20m/min, 5% grade) in older healthy (n=7) and C...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Smith JR,Hageman KS,Harms CA,Poole DC,Musch TI

    更新日期:2017-09-01 00:00:00

  • Postnatal development of eupneic ventilation and metabolism in rats chronically exposed to moderate hyperoxia.

    abstract::Newborn rats chronically exposed to moderate hyperoxia (60% O2) exhibit abnormal respiratory control, including decreased eupneic ventilation. To further characterize this plasticity and explore its proximate mechanisms, rats were exposed to either 21% O2 (Control) or 60% O2 (Hyperoxia) from birth until studied at 3-1...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Bavis RW,van Heerden ES,Brackett DG,Harmeling LH,Johnson SM,Blegen HJ,Logan S,Nguyen GN,Fallon SC

    更新日期:2014-07-01 00:00:00

  • Orexinergic modulation of breathing across vigilance states.

    abstract::Basal respiration and respiratory reflex regulations are considerably different during the awake and sleep states. Tidal volume and respiratory frequency diminish during sleep, and hypoxic and hypercapnic ventilatory responses also decline during sleep. Reduced metabolic demand during sleep cannot completely explain t...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审


    authors: Kuwaki T

    更新日期:2008-12-10 00:00:00

  • Nasal high-frequency oscillatory ventilation inhibits gastroesophageal reflux in the neonatal period.

    abstract::Nasal high-frequency oscillatory ventilation (nHFOV) in neonates is increasingly considered due to enhanced alveolar ventilation, absence of patient-ventilator asynchrony and lessened ventilator-induced lung injury. Although any type of non-invasive respiratory support can lead to gastric distension via esophageal air...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Cantin D,Djeddi D,Samson N,Nadeau C,Praud JP

    更新日期:2018-05-01 00:00:00

  • Medullary serotonin neurons and central CO2 chemoreception.

    abstract::Serotonergic (5-HT) neurons are putative central respiratory chemoreceptors, aiding in the brain's ability to detect arterial changes in PCO2 and implement appropriate ventilatory responses to maintain blood homeostasis. These neurons are in close proximity to large medullary arteries and are intrinsically chemosensit...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审


    authors: Corcoran AE,Hodges MR,Wu Y,Wang W,Wylie CJ,Deneris ES,Richerson GB

    更新日期:2009-08-31 00:00:00

  • Adenosine A2A receptors interact with GABAergic pathways to modulate respiration in neonatal piglets.

    abstract::GABA and adenosine contribute to respiratory inhibition in early postnatal life. In this study the adenosine A2A receptor agonist CGS21680 was used to evaluate adenosine receptor specificity and the interrelation of adenosine and GABA in the inhibition of inspiratory drive. In neonatal piglets (n = 10), CGS21680 was i...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Wilson CG,Martin RJ,Jaber M,Abu-Shaweesh J,Jafri A,Haxhiu MA,Zaidi S

    更新日期:2004-07-20 00:00:00

  • Effects of bi-level positive airway pressure on ventilatory and perceptual responses to exercise in comorbid heart failure-COPD.

    abstract::This study tested the hypothesis that, by increasing the volume available for tidal expansion (inspiratory capacity, IC), bi-level positive airway pressure (BiPAP™) would lead to greater beneficial effects on dyspnea and exercise intolerance in comorbid heart failure (HF)-chronic obstructive pulmonary disease (COPD) t...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Souza A,Sperandio PA,Mazzuco A,Alencar MCN,Arbex FF,Oliveira M,Medeiros W,Rocha A,Nery LE,O Donnell DE,Neder JA

    更新日期:2019-08-01 00:00:00

  • Site of inflammation influences site of hyperresponsiveness in experimental asthma.

    abstract:BACKGROUND:Our recently developed murine asthma model is capable of inducing airway-specific chronic inflammatory changes and remodeling, features of human asthma commonly missing in conventional animal models. OBJECTIVES:To validate this model by site-specific physiological evaluation of hyperresponsiveness. METHODS...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Collins RA,Sly PD,Turner DJ,Herbert C,Kumar RK

    更新日期:2003-12-16 00:00:00

  • Dynamic pulmonary hyperinflation occurs without expiratory flow limitation in chronic heart failure during exercise.

    abstract::To assess the occurrence of tidal expiratory flow limitation (EFL) and/or dynamic pulmonary hyperinflation (DH) in chronic heart failure (CHF) during exercise 15 patients with stable systolic CHF, aged 69 ± 6yr, underwent pulmonary function testing and incremental cardio-pulmonary exercise testing. They subsequently p...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Chiari S,Torregiani C,Boni E,Bassini S,Vizzardi E,Tantucci C

    更新日期:2013-10-01 00:00:00

  • SiO2-induced release of sVEGFRs from pulmonary macrophages.

    abstract:BACKGROUND:The inhalation of silicon dioxide (SiO2) particles causes silicosis, a stubborn pulmonary disease that is characterized by alveolar inflammation during the early stage. Soluble cytokine receptors (SCRs) play important roles in regulating inflammation by either attenuating or promoting cytokine signaling. How...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Chao J,Lv Y,Chen J,Wang J,Yao H

    更新日期:2018-01-01 00:00:00

  • An acute exposure to intermittent negative airway pressure elicits respiratory long-term facilitation in awake humans.

    abstract:BACKGROUND:A sustained elevation in respiratory drive following removal of the inducing stimulus is known as respiratory long-term facilitation (rLTF). We investigated whether an acute exposure to intermittent negative airway pressure (INAP) elicits rLTF in humans. METHOD:13 healthy males (20.9 ± 2.8 years) undertook ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Griffin HS,Al Humoud S,Benson JG,Cooper BG,Coomaraswamy K,Balanos GM

    更新日期:2019-09-01 00:00:00

  • Hypoxic incubation creates differential morphological effects during specific developmental critical windows in the embryo of the chicken (Gallus gallus).

    abstract::Hypoxia inhibits vertebrate development, but the magnitude and timing of organ-specific effects are poorly understood. Chick embryos were exposed continuously to hypoxia (15% O2) throughout Days 1-6, 6-12, 12-18 or Days 1-18 of development, followed by morphometric measurements of major organ systems. Early hypoxic ex...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Chan T,Burggren W

    更新日期:2005-02-15 00:00:00

  • Multiple neural circuits mediating airway sensations: Recent advances in the neurobiology of the urge-to-cough.

    abstract::The respiratory system is densely innervated by sensory neurons arising from the jugular (superior) and nodose (inferior) vagal ganglia. However, a distinction exists between jugular and nodose neurons as these ganglia developmentally originate from the neural crest and the epibranchial placodes, respectively. This di...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审


    authors: Driessen AK,Farrell MJ,Mazzone SB,McGovern AE

    更新日期:2016-06-01 00:00:00

  • Developmental trajectories, critical windows and phenotypic alteration during cardio-respiratory development.

    abstract::Embryo-environment interactions affecting cardio-respiratory development in vertebrates have been extensively studied, but an equally extensive conceptual framework for interpreting and interrelating these developmental events has lagged behind. In this review, we consider the conceptual constructs of "developmental p...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审


    authors: Burggren WW,Reyna KS

    更新日期:2011-08-31 00:00:00

  • Relaxin-3 receptor (RXFP3) activation in the nucleus of the solitary tract modulates respiratory rate and the arterial chemoreceptor reflex in rat.

    abstract::The neuropeptide relaxin-3 is expressed by the pontine nucleus incertus. Relaxin-3 and synthetic agonist peptides modulate arousal and cognitive processes via activation of the relaxin-family peptide 3 receptor (RXFP3). Despite the presence of RXFP3 in the nucleus of the solitary tract (NTS), the ability of RXFP3 to m...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Furuya WI,Dhingra RR,Gundlach AL,Hossain MA,Dutschmann M

    更新日期:2020-01-01 00:00:00

  • Respiratory responses to olfactory stimuli in Parkinson's disease.

    abstract::There are many reports of olfactory impairment in patients with Parkinson's disease (PD) and the impairment can be observed before the appearance of typical PD symptom. Accordingly, olfactory screening tests may predict disease onset and indicates a need for early treatment before classic signs of the disease. Olfacti...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Masaoka Y,Satoh H,Kawamura M,Homma I

    更新日期:2008-04-30 00:00:00

  • Respiratory response to systemic inhibition of the Na+/H+ exchanger type 3 in intact rats.

    abstract::The Na+/H+ exchangers (NHEs) are a family of antiporters involved in the maintenance of neural steady-state intracellular pH. The NHE3 seems to be the predominant subtype in central chemosensitive cells. We aimed to analyze the effect of a selective NHE3 inhibition on the respiratory pattern in spontaneously breathing...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Ribas-Salgueiro JL,Matarredona ER,Sarmiento M,Ribas J,Pásaro R

    更新日期:2009-02-28 00:00:00

  • Blood gases and cardiovascular shunt in the South American lungfish (Lepidosiren paradoxa) during normoxia and hyperoxia.

    abstract::The South American lungfish (Lepidosiren paradoxa) has an arterial P(O(2)) (Pa(O(2))) as high as 70-100 mmHg, corresponding to Hb-O(2) saturations from 90% to 95%, which indicates a moderate cardiovascular right to left (R-L) shunt. In hyperoxia (50% O(2)), we studied animals in: (1) aerated water combined with aerial...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Bassi M,Giusti H,da Silva GS,Amin-Naves J,Glass ML

    更新日期:2010-08-31 00:00:00

  • Influence of dietary nitrate supplementation on physiological and cognitive responses to incremental cycle exercise.

    abstract::Dietary inorganic nitrate supplementation causes physiological effects which may enhance exercise tolerance. However it is not known whether nitrate might alter cognitive function during exercise. In a double-blind, cross-over study, sixteen subjects ingested either nitrate-rich beetroot juice or a placebo and complet...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Thompson KG,Turner L,Prichard J,Dodd F,Kennedy DO,Haskell C,Blackwell JR,Jones AM

    更新日期:2014-03-01 00:00:00

  • Impact of short term forced oral breathing induced by nasal occlusion on respiratory function in mice.

    abstract::Inconsistent findings regarding the experimental nasal obstruction on respiratory functions in small animals have been reported. The purpose of this study was to investigate the impact of short term forced oral breathing on respiratory functions as well as the therapeutic implication of esophageal intubation in BALB/c...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Xie J,Xi Y,Zhang Q,Lai K,Zhong N

    更新日期:2015-01-01 00:00:00

  • Non-invasive CPAP in mild and moderate ARDS secondary to SARS-CoV-2.

    abstract:BACKGROUND:During the COVID-19 outbreak, a very high number of infected patients developed pneumonia and many of them complicated with acute respiratory distress syndrome. The optimal management of respiratory failure and the role of lung ultrasound imaging in the evaluation of efficacy of treatment are unknown. METHO...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Pagano A,Porta G,Bosso G,Allegorico E,Serra C,Dello Vicario F,Minerva V,Russo T,Altruda C,Arbo P,Mercurio V,Numis FG

    更新日期:2020-09-01 00:00:00

  • Reflex inhibition of human inspiratory muscles in response to contralateral phrenic nerve stimulation.

    abstract::In animals, high-intensity unilateral stimulation of the phrenic nerve results in short-latency inhibition of phrenic and intercostal nerve activity bilaterally. This study provides the first demonstration in human subjects of a short-latency inhibitory response in the contralateral scalene, parasternal intercostal an...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 临床试验,杂志文章


    authors: Butler JE,McKenzie DK,Gandevia SC

    更新日期:2003-10-16 00:00:00

  • Arousal and ventilatory responses to hypoxia in sleeping infants: effects of maternal smoking.

    abstract::Our aim was to determine whether maternal cigarette smoking affects arousal and ventilatory responses to hypoxia in infants. Infants born to non-smoking (NS, n = 15) and smoking mothers (SM, n= 9) were studied at 2-5 weeks, 2-3 and 5-6 months. Ventilatory responses to 15% O(2) were determined preceding arousal. At eac...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 临床试验,杂志文章


    authors: Parslow PM,Cranage SM,Adamson TM,Harding R,Horne RS

    更新日期:2004-04-20 00:00:00

  • Spinal vascular endothelial growth factor (VEGF) and erythropoietin (EPO) induced phrenic motor facilitation after repetitive acute intermittent hypoxia.

    abstract::Vascular endothelial growth factor (VEGF) and erythropoietin (EPO) exert neurotrophic and neuroprotective effects in the CNS. We recently demonstrated that VEGF, EPO and their receptors (VEGF-R2, EPO-R) are expressed in phrenic motor neurons, and that cervical spinal VEGF-R2 and EPO-R activation elicit long-lasting ph...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Dale EA,Mitchell GS

    更新日期:2013-02-01 00:00:00

  • Haematological acclimation and re-acclimation to hypoxia in the mouse.

    abstract::Haematological responses throughout 4 w of initial acclimation (IA) and three paradigms of re-acclimation (RA) to hypoxia (FI(O₂)) were examined in female mice. We hypothesised that (i) haematological responses would be increased during re-exposure, resulting in greater O₂-carrying capacity in RA compared to IA; and (...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: MacNutt MJ,Rupert JL,Sheel AW

    更新日期:2013-10-01 00:00:00

  • Posture primarily affects lung tissue distribution with minor effect on blood flow and ventilation.

    abstract::We used quantitative single photon emission computed tomography to estimate the proportion of the observed redistribution of blood flow and ventilation that is due to lung tissue shift with a change in posture. Seven healthy volunteers were studied awake, breathing spontaneously. Regional blood flow and ventilation we...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Petersson J,Rohdin M,Sánchez-Crespo A,Nyrén S,Jacobsson H,Larsson SA,Lindahl SG,Linnarsson D,Neradilek B,Polissar NL,Glenny RW,Mure M

    更新日期:2007-06-15 00:00:00

  • Effects of prolonged exercise to exhaustion on left-ventricular function and pulmonary gas exchange.

    abstract::The purpose of this study was to simultaneously examine left-ventricular (LV) function and pulmonary gas exchange during prolonged constant-rate cycling in an attempt to explain the exercise-induced impairment in gas exchange. Eleven competitive cyclists rode their racing bicycles on a computerized cycle trainer at 25...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Stickland MK,Anderson WD,Haykowsky MJ,Welsh RC,Petersen SR,Jones RL

    更新日期:2004-09-15 00:00:00

  • Airflow pattern complexity during resting breathing in patients with COPD: effect of airway obstruction.

    abstract::We investigated the influence of airway obstruction in the complexity of the airflow pattern in COPD and its use as a marker of disease activity. The sample entropy (SampEnV') and the variability (SDV') of the airflow pattern were measured in a group of 88 subjects with various levels of airway obstruction. Airway obs...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章


    authors: Dames KK,Lopes AJ,de Melo PL

    更新日期:2014-02-01 00:00:00