Abstract：
:Loss of alveolar barrier function is important in the development of pulmonary edema, but quantitation of its integrity has been difficult in the intact lung. We report a new non-radioactive method to assess paracellular and transcellular permeability of alveolar barrier in buffer-perfused rabbit lungs. Changes in alveolar barrier parameters were then correlated with different types of lung edema formation. The paracellular and transcellular barrier function was quantified by calculating the apparent epithelial permeability-surface area products (PS) for a fluorescent hydrophilic solute, FITC-dextran (FD-4), and a hydrophobic solute, rhodamine B, respectively. In control lungs, the apparent epithelial PS for FD-4 and rhodamine B were 0.7+/-0.05 x 10(-4) and 40.0+/-4.1 x 10(-4) ml/sec, respectively. The apparent epithelial PS of FD-4 could be increased 25-fold by inhibition of epithelial anion exchange with 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) without affecting the PS of rhodamine B. The apparent epithelial PS of FD-4 could be increased 6- and 1.7-fold by disrupting microtubules with nocodazole and colchicine respectively, but microtubule agents decreased PS for rhodamine B. A pattern similar was produced when ATP production in the lung was inhibited by 2-deoxyglucose or when oxidative injury was induced by ischemia-reperfusion. Neither DIDS nor nocodazole altered endothelial permeability to albumin. DIDS, but not nocodazole, increased transcapillary liquid filtration and calculated interstitial compliance of the lung during hydrostatic challenge. We conclude that epithelial permeability in the intact lung can be assessed using fluorescent solutes, and that increased permeation of hydrophilic solutes may enhance lung edema formation.
journal_name
Respir Physiol Neurobioljournal_title
Respiratory physiology & neurobiologyauthors
Huang YC,Piantadosi CAdoi
10.1016/s1569-9048(02)00150-7keywords:
subject
Has Abstractpub_date
2002-10-23 00:00:00pages
153-66issue
1-2eissn
1569-9048issn
1878-1519pii
S1569904802001507journal_volume
133pub_type
杂志文章abstract：:This article constitutes a review of the studies performed by the group of the late A. Guz and other authors on the subjects of lung reflexes and perceptions of respiration. The experimental data suggest that the lung inflation and deflation reflexes are present in man, mediated by large myelinated afferent nerve fibr...
journal_title：Respiratory physiology & neurobiology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.resp.2015.06.005
更新日期：2015-10-01 00:00:00
abstract：:Increased transdiaphragmatic pressure, reduced muscle blood flow, and increased duty cycle have all been associated with a reduction in the center frequency (CFdi) of the diaphragm's electrical activity (EAdi). However, the specific influence of diaphragm activation on CFdi is unknown. We evaluated whether increased d...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2004.11.006
更新日期：2005-03-01 00:00:00
abstract：:Dyspnoea, the perception of an unpleasant and/or uncomfortable sensation of breathlessness, offers several physiological, anatomical and teleological analogies with pain. Pain perception has been shown to exist in the newborn, suggesting that dyspnoea may also occur from birth onwards. The perception of breathlessness...
journal_title：Respiratory physiology & neurobiology
pub_type： 社论,评审
doi：10.1016/j.resp.2008.12.001
更新日期：2009-05-30 00:00:00
abstract：:Dyspnea on exertion (DOE) is a common symptom in obesity. We investigated whether aerobic exercise training without weight loss could reduce DOE. Twenty-two otherwise healthy obese women participated in a 12-week supervised aerobic exercise training program, exercising 30 min/day at 70-80% heart rate reserve, 4 days/w...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2015.11.004
更新日期：2016-01-15 00:00:00
abstract：:The objective was to compare the metabolic influence of varying work-to-rest ratios during upper body sprint interval training (SIT). Forty-two recreationally-trained men were randomized into a training group [10 s work - 2 min of rest (10:2) or 4 min of rest (10:4), or 30 s work - 4 min of rest (30:4)] or a control g...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.resp.2019.01.005
更新日期：2019-04-01 00:00:00
abstract：BACKGROUND:The inhalation of silicon dioxide (SiO2) particles causes silicosis, a stubborn pulmonary disease that is characterized by alveolar inflammation during the early stage. Soluble cytokine receptors (SCRs) play important roles in regulating inflammation by either attenuating or promoting cytokine signaling. How...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2017.08.015
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:The purpose of this study was to explore the effect of Wnt pathway on the inhibition of airway epithelial cells repair by glucocorticoid. MATERIALS AND METHODS:The expression of E-cadherin in asthma mice model was detected by immunocytochemistry. XAV939 was used to treat 16HBE, and the expressions of relate...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2019.103283
更新日期：2020-01-01 00:00:00
abstract：:Intermittent hypobaric hypoxia (IHH) exposure, accompanied or not with active recovery, can help to skeletal muscle repair. However, the erythropoietic response elicited can disturb blood rheology and thus alter the oxygen delivery to tissues. Male Sprague-Dawley rats were studied in two basal states: untrained and tr...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2013.12.011
更新日期：2014-02-01 00:00:00
abstract：:Hyperventilation is one way to cause activation on the electroencephalogram (EEG) to diagnose brain disorders. The hyperventilation is also known to affect on the delta power in EEG. This study divided the total delta wave into low, middle, and high bands corresponding to the wave frequency. The power in these three d...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2012.02.010
更新日期：2012-04-30 00:00:00
abstract：:The neonatal rodent serves as useful and appropriate model within which to study respiratory system development. Despite an extensive literature that documents respiratory control in vitro, in vivo studies have relied upon whole body plethysmography to determine measures of respiratory frequency and tidal volume. Howe...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2014.07.018
更新日期：2014-10-01 00:00:00
abstract：:Chronic exposure to sustained hyperoxia alters the development of the respiratory control system, but the respiratory effects of chronic intermittent hyperoxia have rarely been investigated. We exposed newborn rats to short, repeated bouts of 30% O2 or 60% O2 (5 bouts h(-1)) for 4-15 days and then assessed their hypox...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2015.09.015
更新日期：2016-01-01 00:00:00
abstract：:A computational fluid dynamics (CFD) modelling approach is used to study the unsteady respiratory airflow dynamics within a human lung. The three-dimensional asymmetric bifurcation model of the central airway based on the morphological data given by Horsfield et al. (J. Appl. Physiol. 67 (1971) 207) was used in the pr...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/s0034-5687(01)00337-1
更新日期：2002-04-01 00:00:00
abstract：:The respiratory system is a central part of voice production, but for phonation neither the underlying functional relations between diaphragm (DPH) and rib cage (RC), nor differences to normal breathing are yet understood. This study aims to compare respiratory dynamics in phonation and breathing via dynamic MRI of th...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2016.11.007
更新日期：2017-02-01 00:00:00
abstract：:Recent studies in vivo and in vitro suggest that both respiratory rhythmogenesis and its central chemosensory modulation arise from multiple, mechanistically and/or anatomically distinct networks whose outputs are similar. These observations are consistent with degeneracy, defined as the ability of structurally distin...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2010.04.013
更新日期：2010-06-30 00:00:00
abstract：:While a large surface area combined with short diffusion distances make fish gills well suited for gas exchange, these properties leads to costly water and ion fluxes and exposure to toxic substances and pathogens. Thus, gill morphology is likely to be a compromise between opposing demands. It has become clear that so...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2006.02.006
更新日期：2006-11-01 00:00:00
abstract：:Recently, we reported that dyspnea on exertion is strongly associated with an increased oxygen cost of breathing in otherwise healthy obese women; the mechanism of dyspnea on exertion in obese men is unknown. Obese men underwent measurements of body composition, fat distribution, pulmonary function, steady state and m...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2012.10.007
更新日期：2013-01-15 00:00:00
abstract：:Lung ventilation is a mechanical process in which the respiratory muscles are acting in concert to remove air in and out of the lungs. Any alteration in the performance of the respiratory muscle may reduce the effectiveness of ventilation. Thus, early diagnosis of their weakness is vital for treatment and rehabilitati...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2008.04.019
更新日期：2008-11-30 00:00:00
abstract：:Cardiogenic oscillations are small waves produced by heartbeats, which are superimposed on the pressure and flow signals at the airway opening. The aim of this study was to investigate the role of the two main factors believed to generate these oscillations: (1) contact between heart and lungs and (2) pulmonary blood ...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2009.04.026
更新日期：2009-07-31 00:00:00
abstract：:Evidence supports the importance of ovarian hormones as potential tools against sleep apneas in women. On one hand, progesterone is largely acknowledged as being a respiratory stimulant that reduces the frequency of apneas, but the underlying mechanisms remain poorly understood. Recent studies in mice showed that the ...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2017.01.013
更新日期：2017-05-01 00:00:00
abstract：:Basal respiration and respiratory reflex regulations are considerably different during the awake and sleep states. Tidal volume and respiratory frequency diminish during sleep, and hypoxic and hypercapnic ventilatory responses also decline during sleep. Reduced metabolic demand during sleep cannot completely explain t...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2008.03.011
更新日期：2008-12-10 00:00:00
abstract：:Antioxidant therapy attenuates pulmonary hypertension (PH). In the present study, we tested the antioxidant effects of fasudil against PH in rats. Monocrotaline (MCT)-induced and chronic hypoxia-induced PH models of rats were established, and the haemodynamic and pathomorphologic results of three different doses of fa...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2014.06.001
更新日期：2014-09-15 00:00:00
abstract：:Pulmonary mechanics [static (Est) and dynamic (Edyn) elastances, resistive (DeltaP1) and viscoelastic pressures (DeltaP2)], histology, and bronchoalveolar lavage fluid (BALF) from BALB/c mice were analysed 1, 24, 48 and 72 h after intravenous injection of saline or Bothrops jararaca crude venom [0.3 (V0.3) or 1 (V1) m...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2003.10.002
更新日期：2004-01-15 00:00:00
abstract：:Many lung and central nervous system disorders require robust and appropriate physiological responses to assure adequate breathing. Factors undermining the efficacy of ventilatory control will diminish the ability to compensate for pathology, threatening life itself. Although most of these same disorders are associate...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2011.06.017
更新日期：2011-09-30 00:00:00
abstract：:Chronic mountain sickness (CMS) is considered to be a loss of ventilatory acclimatization to high altitude (>2500m) resulting in marked arterial hypoxemia and polycythemia. This case-control study explores the possibility that sleep-disordered breathing (SDB) and associated oxidative stress contribute to the etiology ...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2013.01.016
更新日期：2013-04-01 00:00:00
abstract：:China had taken strict measures for pandemic 2009 H1N1 infection with enhanced surveillance and hospital isolation since April 2009. In Shenzhen, over 1200 confirmed cases of H1N1 infection were identified. Three young patients died of severe pneumonia. Among them, two boys developed neurological complications. Cytoki...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2010.11.004
更新日期：2011-01-31 00:00:00
abstract：:We compared by non-invasive technique the adaptive response of alveolar capillary network to edemagenic conditions (exercise and high altitude [HA, PIO2 107mmHg] in subjects with different resting sea level (SL) capillary blood volume (normalized to alveolar volume, Vc/Va): Group 1 (N=10, Vc/Va=16.1±6.8ml/L- mean±SD) ...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2013.08.021
更新日期：2014-01-01 00:00:00
abstract：:In animals, high-intensity unilateral stimulation of the phrenic nerve results in short-latency inhibition of phrenic and intercostal nerve activity bilaterally. This study provides the first demonstration in human subjects of a short-latency inhibitory response in the contralateral scalene, parasternal intercostal an...
journal_title：Respiratory physiology & neurobiology
pub_type： 临床试验,杂志文章
doi：10.1016/s1569-9048(03)00161-7
更新日期：2003-10-16 00:00:00
abstract：:Respiratory effects of intracarotid injection of gamma-amino-butyric acid (GABA) were investigated in two groups of rats. In the first group of 12 rats the effects of GABA were checked in the intact state, following bilateral vagotomy and GABA receptor blockade. The second group consisted of five initially vagotomized...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/s1569-9048(02)00021-6
更新日期：2002-08-01 00:00:00
abstract：:The present studies evaluate whether the vagus nerves link the lungs' immune and neural systems by transmitting information through pulmonary nociceptors. Single unit activities from pulmonary nociceptors [C fiber receptors (CFRs) and high threshold Adelta fiber receptors (HTARs)] were recorded from the cervical vagus...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2006.11.005
更新日期：2007-05-14 00:00:00
abstract：:Using in vitro newborn rat brainstem-spinal cord preparations with and without the parafacial respiratory group (pFRG), we examined the effects of the volatile anaesthetic sevoflurane on the respiratory rhythm oscillators of the pFRG and the preBötzinger complex (preBötC). Our study indicated that sevoflurane depresse...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2010.06.016
更新日期：2010-08-31 00:00:00
