Abstract：
BACKGROUND:The purpose of this study was to explore the effect of Wnt pathway on the inhibition of airway epithelial cells repair by glucocorticoid. MATERIALS AND METHODS:The expression of E-cadherin in asthma mice model was detected by immunocytochemistry. XAV939 was used to treat 16HBE, and the expressions of related genes were determined by western blotting and quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, migration and cell cycle were analyzed by methylthiazolyldiphenyl-tetrazolium bromide, wound healing and flow cytometry, respectively. RESULTS:In asthma mice model, the lung tissue was impaired. After dexamethasone treatment, the airway inflammation was relieved and the expression of E-cadherin was reduced. Dexamethasone increased the expressions of Wnt7b, LRP5, β-catenin and CyclinD1, inhibited cell viability and migration and arrested cell cycle, whereas XAV939 produced the opposite effects. In addition, XAV939 suppressed Wnt pathway that activated by dexamethasone. CONCLUSION:Glucocorticoid could inhibit cell proliferation and migration via regulating Wnt pathway to affect cell cycle, thus inhibiting the repair of airway epithelial after injury.
journal_name
Respir Physiol Neurobioljournal_title
Respiratory physiology & neurobiologyauthors
Yu Z,Jiang Y,Sun Cdoi
10.1016/j.resp.2019.103283subject
Has Abstractpub_date
2020-01-01 00:00:00pages
103283eissn
1569-9048issn
1878-1519pii
S1569-9048(19)30149-1journal_volume
271pub_type
杂志文章abstract：:A deficiency of surfactant proteins A and D has been proposed as a mechanism in airway remodeling, which is one characteristic of chronic obstructive pulmonary disease (COPD). We recently showed that in vitro nicotine exposure induces Wnt3a/β-catenin activation, which is a pathway that has also been implicated in alte...
journal_title：Respiratory physiology & neurobiology
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journal_title：Respiratory physiology & neurobiology
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abstract：:Angiotensin II (Ang II) has been demonstrated as a pro-inflammatory effect in acute lung injury, but studies of the effect of Ang II on the formation of pulmonary edema and alveolar filling remains unclear. Therefore, in this study the regulation of alveolar fluid clearance (AFC) and the expression of epithelial sodiu...
journal_title：Respiratory physiology & neurobiology
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journal_title：Respiratory physiology & neurobiology
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journal_title：Respiratory physiology & neurobiology
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
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更新日期：2016-01-01 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
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journal_title：Respiratory physiology & neurobiology
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journal_title：Respiratory physiology & neurobiology
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
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更新日期：2007-10-15 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2012.10.014
更新日期：2013-02-01 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2006.08.007
更新日期：2007-04-16 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
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更新日期：2013-10-01 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2013.05.019
更新日期：2013-11-01 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
doi：10.1016/j.resp.2009.11.006
更新日期：2010-01-31 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
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更新日期：2003-09-16 00:00:00
abstract：:These experiments were designed to determine the effects of EEG state on the response of rats to intermittent hypoxia and to test the hypotheses that short-term potentiation (STP) and ventilatory long term facilitation (vLTF) are state dependent; and that neurons with NMDA receptors in the dorso-ventral pontine respir...
journal_title：Respiratory physiology & neurobiology
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更新日期：2010-04-30 00:00:00
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doi：10.1016/s1569-9048(03)00027-2
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journal_title：Respiratory physiology & neurobiology
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更新日期：2014-03-01 00:00:00
abstract：:Although dyspnoea has been shown to attenuate pain, whether different forms of dyspnoea exert a similar inhibitory effect on pain has never been tested. We examined the effects of two different forms of dyspnoea, i.e., "air hunger" sensation (AIR HUNGER) and "work/effort" sensation (WORK/EFFORT), on pain induced by a ...
journal_title：Respiratory physiology & neurobiology
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doi：10.1016/j.resp.2011.05.013
更新日期：2011-08-15 00:00:00
abstract：:Bronchospasm may be part of the response to systemic anaphylaxis in humans. The anaphylactic shock has been characterized in allergic rats, but little data are available on the concurrent changes in airway-lung mechanics. The aim was to describe the respiratory resistance (Rrs) and reactance (Xrs) response to ovalbumi...
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更新日期：2013-10-01 00:00:00
abstract：:The aim of the present paper was to conduct a narrative review of the published norms of the "spirometric" lung-age (SLA). A literature search which covered the period 1970 to June 2017, was conducted using the Pubmed. The search strategy had used the following MeSH words: "Spirometry"[Majr]) AND "Aging"[Majr]. Six or...
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pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
abstract：:The avian embryo toward end-incubation combines gas exchange through the chorioallantoic membrane (CAM) and pulmonary ventilation (V˙E). The main experiments examined breathing activity during cold-hypometabolism. Chicken embryos close to hatching were prepared for simultaneous measurements of oxygen consumption ( [Fo...
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更新日期：2017-08-01 00:00:00
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更新日期：2010-04-15 00:00:00
abstract：:In previous calculations of how the O2 transport system limits .VO2(max), it was reasonably assumed that mitochondrial P(O2) (Pm(O2)) could be neglected (set to zero). However, in reality, Pm(O2) must exceed zero and the red cell to mitochondrion diffusion gradient may therefore be reduced, impairing diffusive transpo...
journal_title：Respiratory physiology & neurobiology
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更新日期：2013-12-01 00:00:00
abstract：BACKGROUND:Recently, surface EMG of parasternal intercostal muscle has been incorporated in the "ERS Statement of Respiratory Muscle Testing" as a clinical technique to monitor the neural respiratory drive (NRD). However, the anatomy of the parasternal muscle risks confounding EMG "crosstalk" activity from neighboring ...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章
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更新日期：2020-11-05 00:00:00
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journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2007.01.013
更新日期：2007-07-01 00:00:00
abstract：:Breathing movements require the coordinated recruitment of cranial and spinal motoneurons innervating muscles of the upper airway and ribcage. A significant part of respiratory motoneuron development and maturation occurs prenatally to support the generation of fetal breathing movements in utero and sustained breathin...
journal_title：Respiratory physiology & neurobiology
pub_type： 杂志文章,评审
doi：10.1016/j.resp.2005.03.017
更新日期：2005-11-15 00:00:00
abstract：:This investigation examined the effects of a six-week inspiratory resistance training (IRT) on metabolic health biomarkers, pulmonary function, and endurance in obese individuals. Twenty-eight obese adults (BMI > 27 kg/m2 Taiwan obesity criteria) were randomly assigned to either IRT (IRT; N = 16) or sham control (PLA;...
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pub_type： 杂志文章,随机对照试验
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更新日期：2020-02-01 00:00:00
