Abstract:
:Individuals with congenital or acquired prolongation of the QT interval, or long QT syndrome (LQTS), are at risk of life-threatening ventricular arrhythmia. LQTS is commonly genetic in origin but can also be caused or exacerbated by environmental factors. A missense mutation in the L-type calcium channel Ca(V)1.2 leads to LQTS in patients with Timothy syndrome. To explore the effect of the Timothy syndrome mutation on the electrical activity and contraction of human cardiomyocytes, we reprogrammed human skin cells from Timothy syndrome patients to generate induced pluripotent stem cells, and differentiated these cells into cardiomyocytes. Electrophysiological recording and calcium (Ca(2+)) imaging studies of these cells revealed irregular contraction, excess Ca(2+) influx, prolonged action potentials, irregular electrical activity and abnormal calcium transients in ventricular-like cells. We found that roscovitine, a compound that increases the voltage-dependent inactivation of Ca(V)1.2 (refs 6-8), restored the electrical and Ca(2+) signalling properties of cardiomyocytes from Timothy syndrome patients. This study provides new opportunities for studying the molecular and cellular mechanisms of cardiac arrhythmias in humans, and provides a robust assay for developing new drugs to treat these diseases.
journal_name
Naturejournal_title
Natureauthors
Yazawa M,Hsueh B,Jia X,Pasca AM,Bernstein JA,Hallmayer J,Dolmetsch REdoi
10.1038/nature09855subject
Has Abstractpub_date
2011-03-10 00:00:00pages
230-4issue
7337eissn
0028-0836issn
1476-4687pii
nature09855journal_volume
471pub_type
杂志文章相关文献
NATURE文献大全abstract::At one time the synthetic chemist's last resort, reactions catalysed by transition metals are now the preferred method for synthesizing many types of organic molecule. A recent success in this type of catalysis is the discovery of reactions that form bonds between carbon and heteroatoms (such as nitrogen, oxygen, sulp...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/nature07369
更新日期:2008-09-18 00:00:00
abstract::Inherited genetic variation has a critical but as yet largely uncharacterized role in human disease. Here we report a public database of common variation in the human genome: more than one million single nucleotide polymorphisms (SNPs) for which accurate and complete genotypes have been obtained in 269 DNA samples fro...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04226
更新日期:2005-10-27 00:00:00
abstract::Direct dating of ductile shear zones and calculation of uplift/exhumation rates can be done using various radiometric dating techniques. But radiometric dating of shallow crustal faulting, which occurs in the crust's brittle regime, has remained difficult because the low temperatures typical of shallow crusted faults ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35084053
更新日期:2001-07-12 00:00:00
abstract::Nuclear pore complexes (NPCs) fuse the inner and outer membranes of the nuclear envelope. They comprise hundreds of nucleoporins (Nups) that assemble into multiple subcomplexes and form large central channels for nucleocytoplasmic exchange1,2. How this architecture facilitates messenger RNA export, NPC biogenesis and ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2670-5
更新日期:2020-10-01 00:00:00
abstract::Arid ecosystems, which occupy about 20% of the earth's terrestrial surface area, have been predicted to be one of the most responsive ecosystem types to elevated atmospheric CO2 and associated global climate change. Here we show, using free-air CO2 enrichment (FACE) technology in an intact Mojave Desert ecosystem, tha...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35040544
更新日期:2000-11-02 00:00:00
abstract::An ageing world population has fuelled interest in regenerative remedies that may stem declining organ function and maintain fitness. Unanswered is whether elimination of intrinsic instigators driving age-associated degeneration can reverse, as opposed to simply arrest, various afflictions of the aged. Such instigator...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09603
更新日期:2011-01-06 00:00:00
abstract::Coherent light sources can be used to manipulate the outcome of light-matter interactions by exploiting interference phenomena in the time and frequency domain. A powerful tool in this emerging field of 'quantum control' is the adaptive shaping of femtosecond laser pulses, resulting, for instance, in selective molecul...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35102037
更新日期:2001-11-01 00:00:00
abstract::Lymphocyte function-associated antigen-1 (LFA-1) is a heterodimer composed of an alpha and beta chain that is expressed on the surface of most leukocytes and is an essential molecule for adhesion reactions between cells participating in the immune response. A putative ligand for LFA-1 is the intercellular adhesion mol...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/333850a0
更新日期:1988-06-30 00:00:00
abstract::Corticosteroids as well as non-steroid anti-inflammatory drugs inhibit the prostaglandin-mediated vasodilatation accompanying lipolysis in subcutaneous fat. Whereas the non-steroids produce their effect by inhibition of prostaglandin synthesis, however, corticosteroids inhibit their release. This mechanism may be the ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/254308a0
更新日期:1975-03-27 00:00:00
abstract::During an immune response, hypermutation of immunoglobulin genes in B cells proliferating within germinal centres (GCs) generates variant antibodies that react with higher affinity against either foreign or self antigens. Several experiments suggest that self-reactive B cells may be censored at this stage of the immun...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/375334a0
更新日期:1995-05-25 00:00:00
abstract::The female germ line undergoes a unique sequence of differentiation processes that confers totipotency to the egg. The reconstitution of these events in vitro using pluripotent stem cells is a key achievement in reproductive biology and regenerative medicine. Here we report successful reconstitution in vitro of the en...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature20104
更新日期:2016-11-10 00:00:00
abstract::The disease non-insulin-dependent (type 2) diabetes mellitus (NIDDM) is characterized by abnormally high blood glucose resulting from a relative deficiency of insulin. It affects about 2% of the world's population and treatment of diabetes and its complications are an increasing health-care burden. Genetic factors are...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/384455a0
更新日期:1996-12-05 00:00:00
abstract::Non-impulsive seismic radiation or 'tremor' has long been observed at volcanoes and more recently around subduction zones. Although the number of observations of non-volcanic tremor is steadily increasing, the causative mechanism remains unclear. Some have attributed non-volcanic tremor to the movement of fluids, whil...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature06017
更新日期:2007-08-02 00:00:00
abstract::Cell-cell interactions are thought to play a crucial part in determining the developmental fate of vertebrate cells and regulating their subsequent differentiation. In the peripheral nervous system, for example, signals from neuronal axons determine whether or not some Schwann cells wrap their plasma membrane concentr...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/309367a0
更新日期:1984-05-24 00:00:00
abstract::Angiosperms developed floral nectaries that reward pollinating insects. Although nectar function and composition have been characterized, the mechanism of nectar secretion has remained unclear. Here we identify SWEET9 as a nectary-specific sugar transporter in three eudicot species: Arabidopsis thaliana, Brassica rapa...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13082
更新日期:2014-04-24 00:00:00
abstract::Processes such as the scattering of alpha particles ((4)He), the triple-alpha reaction, and alpha capture play a major role in stellar nucleosynthesis. In particular, alpha capture on carbon determines the ratio of carbon to oxygen during helium burning, and affects subsequent carbon, neon, oxygen, and silicon burning...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature16067
更新日期:2015-12-03 00:00:00
abstract::Certain cell fate decisions are specified by cell-cell interactions during the development of the nematode Caenorhabditis elegans. For example, in a wild-type hermaphrodite gonad, two cells, Z1.ppp and Z4.aaa, have the potential to become the anchor cell (AC). Intercellular communication establishes their fates and en...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/346197a0
更新日期:1990-07-12 00:00:00
abstract::Global efforts to mitigate climate change are guided by projections of future temperatures. But the eventual equilibrium global mean temperature associated with a given stabilization level of atmospheric greenhouse gas concentrations remains uncertain, complicating the setting of stabilization targets to avoid potenti...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature08019
更新日期:2009-04-30 00:00:00
abstract::Nanofabrication strategies are becoming increasingly expensive and equipment-intensive, and consequently less accessible to researchers. As an alternative, scanning probe lithography has become a popular means of preparing nanoscale structures, in part owing to its relatively low cost and high resolution, and a regist...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09697
更新日期:2011-01-27 00:00:00
abstract::Many-body systems in nature exhibit complexity and self-organization arising from seemingly simple laws. For example, the long-range Coulomb interaction between electrical charges has a simple form, yet is responsible for a plethora of bound states in matter, ranging from the hydrogen atom to complex biochemical struc...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature01676
更新日期:2003-06-12 00:00:00
abstract::A hallmark of RNA silencing is a class of approximately 22-nucleotide RNAs that are processed from double-stranded RNA precursors by Dicer. Accurate processing by Dicer is crucial for the functionality of microRNAs (miRNAs). The current model posits that Dicer selects cleavage sites by measuring a set distance from th...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10198
更新日期:2011-07-13 00:00:00
abstract::Changes in redox status are a conspicuous feature of immune responses in a variety of eukaryotes, but the associated signalling mechanisms are not well understood. In plants, attempted microbial infection triggers the rapid synthesis of nitric oxide and a parallel accumulation of reactive oxygen intermediates, the lat...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10427
更新日期:2011-10-13 00:00:00
abstract::Global conservation strategies commonly assume that different taxonomic groups show congruent geographical patterns of diversity, and that the distribution of extinction-prone species in one group can therefore act as a surrogate for vulnerable species in other groups when conservation decisions are being made. The va...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature05237
更新日期:2006-11-02 00:00:00
abstract::A new gene, termed klotho, has been identified that is involved in the suppression of several ageing phenotypes. A defect in klotho gene expression in the mouse results in a syndrome that resembles human ageing, including a short lifespan, infertility, arteriosclerosis, skin atrophy, osteoporosis and emphysema. The ge...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/36285
更新日期:1997-11-06 00:00:00
abstract::Clonal deletion in the thymus plays a major part in T-cell tolerance to self antigens. But the mechanism of negative selection, its fine specificity and the threshold of affinity and avidity remains unknown. We have now examined these aspects of negative selection with mice expressing a transgenic T-cell receptor with...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/351482a0
更新日期:1991-06-06 00:00:00
abstract::Little is known about metabolic regulation in stem cells and how this modulates tissue regeneration or tumour suppression. We studied the Lkb1 tumour suppressor and its substrate AMP-activated protein kinase (AMPK), kinases that coordinate metabolism with cell growth. Deletion of the Lkb1 (also called Stk11) gene in m...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09571
更新日期:2010-12-02 00:00:00
abstract::The recent discoveries of RNA interference and related RNA silencing pathways have revolutionized our understanding of gene regulation. RNA interference has been used as a research tool to control the expression of specific genes in numerous experimental organisms and has potential as a therapeutic strategy to reduce ...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/nature02872
更新日期:2004-09-16 00:00:00
abstract::Animal behaviour arises from computations in neuronal circuits, but our understanding of these computations has been frustrated by the lack of detailed synaptic connection maps, or connectomes. For example, despite intensive investigations over half a century, the neuronal implementation of local motion detection in t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12450
更新日期:2013-08-08 00:00:00
abstract::TMV RNA is not an efficient template for translation of the viral coat protein, in spite of containing nucleotide sequences coding for the protein. Efficient translation requires the prior synthesis within infected cells of a smaller RNA carrying only a portion of the information encoded in the whole genome. ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/260759a0
更新日期:1976-04-29 00:00:00
abstract:: ...
journal_title:Nature
pub_type: 新闻
doi:10.1038/d41586-019-00121-0
更新日期:2019-01-01 00:00:00