Abstract:
:Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.
journal_name
Naturejournal_title
Natureauthors
Graveley BR,Brooks AN,Carlson JW,Duff MO,Landolin JM,Yang L,Artieri CG,van Baren MJ,Boley N,Booth BW,Brown JB,Cherbas L,Davis CA,Dobin A,Li R,Lin W,Malone JH,Mattiuzzo NR,Miller D,Sturgill D,Tuch BB,Zaleski C,doi
10.1038/nature09715subject
Has Abstractpub_date
2011-03-24 00:00:00pages
473-9issue
7339eissn
0028-0836issn
1476-4687pii
nature09715journal_volume
471pub_type
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