Downregulation of survivin expression and enhanced chemosensitivity of MCF-7 cells to adriamycin by PDMAE/survivin shRNA complex nanoparticles.


:Gene silencing mediated by RNA interference (RNAi) presents a promising strategy for gene therapy. The aim of this work is to evaluate a new gene delivery system for downregulation of survivin expression and enhanced chemosensitivity of MCF-7 cells to adriamycin (ADR). A new cationic poly(2-dimethylaminoethylamine/2-(2-aminoethyoxy)ethoxy)phosphazene (PDMAE) with multiple amino groups was synthesized through Michael addition for survivin shRNA (shSur) delivery in MCF-7 cells. PDMAE51/shSur complex nanoparticles with the size of 190nm and zeta potential of +15mV achieved maximal suppression of survivin, even superior to PEI25K or poly(2-(2-aminoethyoxy)ethoxy)phosphazene (PAEP) based complex nanoparticles. The significant downregulation of survivin expression was achieved by PDMAE51/shSur nanoparticles. The nuclear localization by confocal laser scanning microscopy (CLSM) and apparent apoptosis peak of cell cycle in MCF-7 cells were observed when transfected by PDMAE51/shSur nanoparticles The combined use of PDMAE51/shSur and ADR enhanced the sensitivity of MCF-7 cells to ADR at a larger extent than that of PEI or PAEP based complex nanoparticles. These results suggested that PDMAE51 could be potential as an efficient and safe gene carrier in RNAi therapeutics and tumor chemotherapy.


Int J Pharm


Yang Y,Gao Y,Chen L,Huang Y,Li Y




Has Abstract


2011-02-28 00:00:00














  • Chitosan oligosaccharide enhances binding of nanostructured lipid carriers to ocular mucins: Effect on ocular disposition.

    abstract::The objective of the study was to assess the effect of enhanced mucoadhesion of a cationic mucoadhesive nanostructured lipid carrier (NLC) on its ocular disposition after topical administration. The NLC was made mucoadhesive by surface coating with chitosan oligosaccharide (COS), a low molecular weight derivate of chi...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Pai RV,Vavia PR

    更新日期:2020-03-15 00:00:00

  • A combo-strategy to improve brain delivery of antiepileptic drugs: Focus on BCRP and intranasal administration.

    abstract::The breast cancer resistance protein (BCRP) is an efflux transporter expressed at the apical surface of human brain endothelial cells of the blood-brain barrier (BBB). It was proposed as one of the transporters responsible for the development of drug resistance to several central nervous system (CNS) drugs, including ...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Gonçalves J,Silva S,Gouveia F,Bicker J,Falcão A,Alves G,Fortuna A

    更新日期:2021-01-25 00:00:00

  • Lubrication properties of potential alternative lubricants, glycerin fatty acid esters, to magnesium stearate.

    abstract::To study the usefulness of glycerin fatty acid ester Poem TR-FB (TR-FB) and Poem TR-HB (TR-HB) as lubricants, pressure transmission ratio, ejection force, disintegration time, and tensile strength were measured at different concentrations and mixing times for granules and tablets. When each lubricant was mixed at 0.1-...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Uchimoto T,Iwao Y,Ikegami Y,Murata T,Sonobe T,Miyagishima A,Itai S

    更新日期:2010-02-15 00:00:00

  • Targeting colon cancer cells using PEGylated liposomes modified with a fibronectin-mimetic peptide.

    abstract::Integrin alpha(5)beta(1) is expressed on several types of cancer cells, including colon cancer, and plays an important role in tumor growth and metastasis. The ability to target the integrin alpha(5)beta(1) using an appropriate drug delivery nano-vector can significantly help in inhibiting tumor growth, reducing tumor...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Garg A,Tisdale AW,Haidari E,Kokkoli E

    更新日期:2009-01-21 00:00:00

  • Novel fenofibric acid-loaded controlled release pellet bioequivalent to choline fenofibrate-loaded commercial product in beagle dogs.

    abstract::The objective of this study was to develop a novel fenofibric acid-loaded controlled release pellet showing enhanced, or equivalent to, bioavailability compared with two commercially available products containing fenofibrate or choline fenofibrate. The effect of solubilizing agents on drug solubility and the impact of...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Kim KS,Jin SG,Mustapha O,Yousaf AM,Kim DW,Kim YH,Kim JO,Yong CS,Woo JS,Choi HG

    更新日期:2015-07-25 00:00:00

  • Efficient incorporation and protection of lansoprazole in cyclodextrin metal-organic frameworks.

    abstract::Lansoprazole (LPZ) is an acid pump inhibitor, which readily degrades upon acidic or basic conditions and under heating. We investigated here LPZ stability upon incorporation in particles made of cyclodextrin metal-organic frameworks (CD-MOFs). LPZ loaded CD-MOFs were successfully synthesized, reaching high LPZ payload...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Li X,Porcino M,Martineau-Corcos C,Guo T,Xiong T,Zhu W,Patriarche G,Péchoux C,Perronne B,Hassan A,Kümmerle R,Michelet A,Zehnacker-Rentien A,Zhang J,Gref R

    更新日期:2020-07-30 00:00:00

  • Effect of the surface free energy of materials on the lamination tendency of bilayer tablets.

    abstract::Dosage forms with fixed dose combinations of drugs is a frequent and advantageous mode of administration, but their production involves a number of technological problems. Numerous interactions in a homogeneous vehicle may be avoided through the use of layered tablets. The mechanical properties of these dosage forms d...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Papós K,Kása P Jr,Ilič I,Blatnik-Urek S,Regdon G Jr,Srčič S,Pintye-Hódi K,Sovány T

    更新日期:2015-12-30 00:00:00

  • An investigation into interactions between polyacrylic polymers and a non-ionic surfactant: an emulsion preformulation study.

    abstract::The aim of this study was to investigate possible interactions between a polymeric emulsifier and a non-ionic surfactant, with a view of achieving better understanding of emulsion stabilisation mechanisms. The polymeric emulsifier used was acrylates/C10-30 alkyl acrylate crosspolymer (Pemulen TR-2(R)), while Polyoxyet...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Simovic S,Tamburic S,Milic-Askrabic J,Rajic D

    更新日期:1999-07-20 00:00:00

  • Role of residual solvents in the formation of volatile compounds after radiosterilisation of cefotaxime.

    abstract::Radiation sterilisation is a promising method to sterilise pharmaceutical products. However, this process is accompanied by a modification of odour due to volatile compounds formation. The origin of malodorous compounds produced during solid cefotaxime radiosterilisation has been investigated and several mechanisms ar...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Barbarin N,Rollmann B,Tilquin B

    更新日期:1999-02-15 00:00:00

  • Complexation of cationic-neutral block polyelectrolyte with insulin and in vitro release studies.

    abstract::Insulin (INS) was incorporated into complexes with the block polyelectrolyte quaternized poly[3,5-bis(dimethylaminomethylene)hydroxystyrene]-b-poly(ethylene oxide) (QNPHOSEO), which is a cationic-neutral block polyelectrolyte. Light scattering techniques are used in order to examine the size, the size distribution and...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Pippa N,Karayianni M,Pispas S,Demetzos C

    更新日期:2015-08-01 00:00:00

  • A novel method for encapsulation of poorly water-soluble drugs: precipitation in polyelectrolyte multilayer shells.

    abstract::A novel method to include poorly water-soluble substances into the polyelectrolyte capsules of defined size, stability, composition and affinity properties is proposed. Encapsulation explores the polarity gradient across the capsule wall. Capsules creation makes use of electrostatic interaction and can involve many su...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Radtchenko I,Sukhorukov G,Möhwald H

    更新日期:2002-08-21 00:00:00

  • 3D printing of tablets using inkjet with UV photoinitiation.

    abstract::Additive manufacturing (AM) offers significant potential benefits in the field of drug delivery and pharmaceutical/medical device manufacture. Of AM processes, 3D inkjet printing enables precise deposition of a formulation, whilst offering the potential for significant scale up or scale out as a manufacturing platform...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Clark EA,Alexander MR,Irvine DJ,Roberts CJ,Wallace MJ,Sharpe S,Yoo J,Hague RJM,Tuck CJ,Wildman RD

    更新日期:2017-08-30 00:00:00

  • Squalene-based nanoparticles for the targeting of atherosclerotic lesions.

    abstract::Native low-density lipoproteins (LDL) naturally accumulate at atherosclerotic lesions and are thought to be among the main drivers of atherosclerosis progression. Numerous nanoparticular systems making use of recombinant lipoproteins have been developed for targeting atherosclerotic plaque. These innovative formulatio...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Brusini R,Dormont F,Cailleau C,Nicolas V,Peramo A,Varna M,Couvreur P

    更新日期:2020-05-15 00:00:00

  • Mucoadhesive zein/beta-cyclodextrin nanoparticles for the buccal delivery of curcumin.

    abstract::Herein, we have developed sprayable dispersions of mucoadhesive nanoparticles (NPs) made of zein, a hydrophobic plant-based protein, for the buccal delivery of curcumin (CUR), a poorly water-soluble polyphenol. NPs were prepared by the liquid-liquid dispersion method using an antisolvent water solution without or with...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Esposito D,Conte C,d'Angelo I,Miro A,Ungaro F,Quaglia F

    更新日期:2020-08-30 00:00:00

  • Mechanical particle coating using ethylcellulose nanoparticle agglomerates for preparing controlled release fine particles; effect of coating temperature on coating performance.

    abstract::This study describes the effect of coating temperature on the performance of mechanical particle coating using ethylcellulose, which was done to produce controlled-release particles (diameters less than 100 μm) with different release rates. First, theophylline crystals were spheronized using a mechanical powder proces...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Kondo K,Ando C,Niwa T

    更新日期:2019-01-10 00:00:00

  • Development of self-emulsifying drug delivery systems (SEDDS) for ciprofloxacin with improved mucus permeating properties.

    abstract::The aim of this study was to develop a self-emulsifying drug delivery system (SEDDS) containing the fluoroquinolone antibiotic ciprofloxacin (CIP) exhibiting highly mucus permeating properties and antimicrobial activity in in vitro models. Various SEDDS formulations were developed and evaluated regarding droplet size,...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Zaichik S,Steinbring C,Menzel C,Knabl L,Orth-Höller D,Ellemunter H,Niedermayr K,Bernkop-Schnürch A

    更新日期:2018-08-25 00:00:00

  • A chitosan hydrogel-based cancer drug delivery system exhibits synergistic antitumor effects by combining with a vaccinia viral vaccine.

    abstract::Cancer treatment combining chemotherapy and immunotherapy has been vigorously exploited to further improve cancer therapeutic efficacy. This study investigated a new chemoimmunotherapy approach utilizing hydrogel as a local anti-cancer drug delivery system. Chitosan hydrogel containing doxorubicin (CH-DOX) and vaccini...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Han HD,Song CK,Park YS,Noh KH,Kim JH,Hwang T,Kim TW,Shin BC

    更新日期:2008-02-28 00:00:00

  • Human erythrocytes are affected in vitro by flavonoids of Aristotelia chilensis (Maqui) leaves.

    abstract::Aristotelia chilensis (Mol.) Stuntz (A. chilensis), also known as maqui, is a plant of the Elaeocarpaceae family that grows in central and southern Chile as well as southwestern Argentina. Infusions of its leaves have long been used in the traditional native herbal medicine to treat different ailments. Phytochemical s...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Suwalsky M,Vargas P,Avello M,Villena F,Sotomayor CP

    更新日期:2008-11-03 00:00:00

  • pH-sensitive and mucoadhesive thiolated Eudragit-coated chitosan microspheres.

    abstract::The aim of this study was using Eudragit-cysteine conjugate to coat on chitosan microspheres (CMs) for developing an oral protein drug delivery system, having mucoadhesive and pH-sensitive property. Bovine serum albumin (BSA) as a protein model drug was loaded in thiolated Eudragit-coated CMs (TECMs) to study the rele...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Quan JS,Jiang HL,Kim EM,Jeong HJ,Choi YJ,Guo DD,Yoo MK,Lee HG,Cho CS

    更新日期:2008-07-09 00:00:00

  • A high throughput platform for understanding the influence of excipients on physical and chemical stability.

    abstract::The present study puts forward a miniaturized high-throughput platform to understand influence of excipient selection and processing on the stability of a given drug compound. Four model drugs (sodium naproxen, theophylline, amlodipine besylate and nitrofurantoin) and ten different excipients were selected. Binary phy...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Raijada D,Cornett C,Rantanen J

    更新日期:2013-08-30 00:00:00

  • In vitro and in vivo evaluation of mucoadhesive microspheres consisting of dextran derivatives and cellulose acetate butyrate.

    abstract::The objective of this study was to evaluate mucoadhesive properties and gastrointestinal transit of microspheres made of oppositely charged dextran derivatives and cellulose acetate butyrate (CAB). The microspheres were prepared by emulsion solvent evaporation method. A reference microsphere was made of lactose instea...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Miyazaki Y,Ogihara K,Yakou S,Nagai T,Takayama K

    更新日期:2003-06-04 00:00:00

  • Chitosomes as drug delivery systems for C-phycocyanin: preparation and characterization.

    abstract::The aim of this work was to investigate chitosomes, i.e. liposomes coated by a polyelectrolyte complex between chitosan (CH) and xantan gum (XG), as potential delivery system for oral administration of the protein C-phycocyanin. To this purpose several CH-XG-microcomplexes were prepared in aqueous lactic acid at diffe...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Manconi M,Mura S,Manca ML,Fadda AM,Dolz M,Hernandez MJ,Casanovas A,Díez-Sales O

    更新日期:2010-06-15 00:00:00

  • Highly stable polyglutamate derivatives/siRNA polyplex efficiently down-relegate survivin expression and augment the efficacy of cisplatin.

    abstract::RNA interfere (RNAi)-based technology holds great promise in cancer treatment. The use of small interfering RNA (siRNA), however, is hampered by its low delivery efficiency in vivo when they are diluted in blood biofluids and in the presence of serum and salt. In this study, we developed the polyglutamate derivative p...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Wang Z,Zou H,Wang Z,Wu J,Xia Z,Feng M

    更新日期:2016-05-30 00:00:00

  • Gene expression in an intact ex-vivo skin tissue model following percutaneous delivery of cationic liposome-plasmid DNA complexes.

    abstract::The skin represents an attractive site for the localised gene therapy of dermatological pathologies and as a potential antigen bioreactor following transdermal delivery. Potential also exists for the gene therapy of skin as a cosmetic intervention. The most exploited non-viral gene delivery system involves the complex...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Birchall JC,Marichal C,Campbell L,Alwan A,Hadgraft J,Gumbleton M

    更新日期:2000-03-20 00:00:00

  • Characterization and biological properties of NanoCUR formulation and its effect on major human cytochrome P450 enzymes.

    abstract::Curcumin (CUR) has been formulated into a host of nano-sized formulations in a bid to improve its in vivo solubility, stability and bioavailability. The aim of this study was to investigate whether the encapsulation of CUR in nanocarriers would impede its biological interactivity, specifically its potential anti-cance...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Shamsi S,Chen Y,Lim LY

    更新日期:2015-11-10 00:00:00

  • Search for technological reasons to develop a capsule or a tablet formulation with respect to wettability and dissolution.

    abstract::Proquazone, a poorly wettable compound, was used as a model drug in the search for reasons to develop a capsule or tablet formulation. The capsules were filled with proquazone as active ingredient, with lactose monohydrate (200 mesh) as filler and with magnesium stearate as lubricant. The tablet was made out of a gran...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: von Orelli J,Leuenberger H

    更新日期:2004-12-09 00:00:00

  • Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (A). Mechanisms of interaction.

    abstract::This paper examines a perspective on the use of newly engineered nanomaterials as effective and safe carriers of genes for the therapy of cancer. Three different groups of cationic dendrimers (PAMAM, phosphorus and carbosilane) were complexed with anticancer siRNA and their biophysical properties of the dendriplexes a...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Ionov M,Lazniewska J,Dzmitruk V,Halets I,Loznikova S,Novopashina D,Apartsin E,Krasheninina O,Venyaminova A,Milowska K,Nowacka O,Gomez-Ramirez R,de la Mata FJ,Majoral JP,Shcharbin D,Bryszewska M

    更新日期:2015-05-15 00:00:00

  • Downscaling of the tableting process: Feasibility of miniaturized forced feeders on a high-speed rotary tablet press.

    abstract::With the current transformation of the pharmaceutical industry towards continuous manufacturing, there is an inherent need to embrace this concept already during the early stages of drug formulation. Therefore, this research paper investigated the feasibility of using miniaturized forced feeders on a high-speed rotary...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Grymonpré W,Blahova Prudilova B,Vanhoorne V,Van Snick B,Detobel F,Remon JP,De Beer T,Vervaet C

    更新日期:2018-10-25 00:00:00

  • Formulation and cytotoxicity of doxorubicin nanoparticles carried by dry powder aerosol particles.

    abstract::Regional drug delivery via dry powder inhalers offers many advantages in the management of pharmaceutical compounds for the prevention and treatment of respiratory diseases. In the present study, doxorubicin (DOX)-loaded nanoparticles were incorporated as colloidal drug delivery system into inhalable carrier particles...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Azarmi S,Tao X,Chen H,Wang Z,Finlay WH,Löbenberg R,Roa WH

    更新日期:2006-08-17 00:00:00

  • Skin penetration and deposition of carboxyfluorescein and temoporfin from different lipid vesicular systems: In vitro study with finite and infinite dosage application.

    abstract::The aim of the present research is to evaluate the influence of different lipid vesicular systems as well as the effect of application mode on skin penetration and deposition behaviors of carboxyfluorescein (hydrophilic model drug) and temoporfin (lipophilic model drug). All of the lipid vesicular systems, including c...

    journal_title:International journal of pharmaceutics

    pub_type: 杂志文章


    authors: Chen M,Liu X,Fahr A

    更新日期:2011-04-15 00:00:00